{"id":"https://openalex.org/W2002442479","doi":"https://doi.org/10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.0.co;2-t","title":"Osmolyte-induced changes in protein conformational equilibria","display_name":"Osmolyte-induced changes in protein conformational equilibria","publication_year":2000,"publication_date":"2000-04-05","ids":{"openalex":"https://openalex.org/W2002442479","doi":"https://doi.org/10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.0.co;2-t","mag":"2002442479","pmid":"https://pubmed.ncbi.nlm.nih.gov/10685050"},"language":"en","primary_location":{"id":"doi:10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.0.co;2-t","doi":"https://doi.org/10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.0.co;2-t","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.0.co;2-t","pdf_url":null,"source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061553021","display_name":"Aleister J. Saunders","orcid":"https://orcid.org/0000-0002-7298-7823","observed_orcids":["https://orcid.org/0000-0002-7298-7823"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aleister J. Saunders","raw_affiliation_strings":["Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA","Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053562506","display_name":"Paula R. Davis-Searles","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paula R. Davis-Searles","raw_affiliation_strings":["Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043332053","display_name":"Devon L. Allen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Devon L. Allen","raw_affiliation_strings":["Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076371120","display_name":"Gary J. Pielak","orcid":"https://orcid.org/0000-0001-6307-542X","observed_orcids":["https://orcid.org/0000-0001-6307-542X"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gary J. Pielak","raw_affiliation_strings":["Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599","Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5032779622","display_name":"Dorothy A. Erie","orcid":"https://orcid.org/0000-0003-0363-4693","observed_orcids":["https://orcid.org/0000-0003-0363-4693"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Dorothy A. Erie","raw_affiliation_strings":["Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599","institution_ids":["https://openalex.org/I114027177"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5032779622"],"corresponding_institution_ids":["https://openalex.org/I114027177"],"apc_list":{"value":4150,"currency":"USD","value_usd":4150},"apc_paid":null,"fwci":2.6062,"has_fulltext":false,"cited_by_count":154,"citation_normalized_percentile":{"value":0.89950164,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":99},"biblio":{"volume":"53","issue":"4","first_page":"293","last_page":"293"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/osmolytes","display_name":"osmolytes","score":0.9850000143051147},{"id":"https://openalex.org/keywords/yeast-cytochrome-c","display_name":"yeast cytochrome c","score":0.8370000123977661},{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.5730000138282776},{"id":"https://openalex.org/keywords/excluded-volume","display_name":"excluded volume","score":0.8740000128746033},{"id":"https://openalex.org/keywords/steric-repulsion","display_name":"steric repulsion","score":0.8450000286102295},{"id":"https://openalex.org/keywords/acid-denaturation","display_name":"acid denaturation","score":0.7870000004768372},{"id":"https://openalex.org/keywords/thermal-denaturation","display_name":"thermal denaturation","score":0.9900000095367432},{"id":"https://openalex.org/keywords/scaled-particle-theory","display_name":"scaled particle theory","score":0.7860000133514404},{"id":"https://openalex.org/keywords/protein-hydration","display_name":"protein hydration","score":0.30399999022483826}],"concepts":[{"id":"https://openalex.org/C127160389","wikidata":"https://www.wikidata.org/wiki/Q415608","display_name":"Osmolyte","level":2,"score":0.9640597105026245},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8684995174407959},{"id":"https://openalex.org/C201194858","wikidata":"https://www.wikidata.org/wiki/Q898238","display_name":"Steric effects","level":2,"score":0.762994647026062},{"id":"https://openalex.org/C2988375501","wikidata":"https://www.wikidata.org/wiki/Q847556","display_name":"Protein stability","level":2,"score":0.6509670615196228},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.4039548635482788},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.35499846935272217},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.20218122005462646},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15944617986679077},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000665","descriptor_name":"Ampholyte Mixtures","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D003574","descriptor_name":"Cytochrome c Group","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012996","descriptor_name":"Solutions","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014867","descriptor_name":"Water","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.0.co;2-t","doi":"https://doi.org/10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.0.co;2-t","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.0.co;2-t","pdf_url":null,"source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},{"id":"doi:10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.3.co;2-k","doi":"https://doi.org/10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.3.co;2-k","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1097-0282(20000405)53:4<293::aid-bip2>3.3.co;2-k","pdf_url":null,"source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},{"id":"pmid:10685050","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10685050","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.210.9504","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.210.9504","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.chem.unc.edu/people/faculty/erie/group/publications/biop2000.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G506281390","display_name":null,"funder_award_id":"GM-54136","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5272088139","display_name":null,"funder_award_id":"GM42501","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":78,"referenced_works":["https://openalex.org/W194476900","https://openalex.org/W354005885","https://openalex.org/W409520597","https://openalex.org/W411383580","https://openalex.org/W1487327920","https://openalex.org/W1513861898","https://openalex.org/W1524646116","https://openalex.org/W1534882817","https://openalex.org/W1553590608","https://openalex.org/W1560189074","https://openalex.org/W1562907760","https://openalex.org/W1572204111","https://openalex.org/W1605936672","https://openalex.org/W1827848509","https://openalex.org/W1969939157","https://openalex.org/W1976179026","https://openalex.org/W1979134948","https://openalex.org/W1979675274","https://openalex.org/W1980028982","https://openalex.org/W1981449259","https://openalex.org/W1986115510","https://openalex.org/W1988377264","https://openalex.org/W1990759970","https://openalex.org/W1993327079","https://openalex.org/W2000943591","https://openalex.org/W2006588066","https://openalex.org/W2009847304","https://openalex.org/W2009857351","https://openalex.org/W2011494651","https://openalex.org/W2015176397","https://openalex.org/W2023159932","https://openalex.org/W2024457804","https://openalex.org/W2027886127","https://openalex.org/W2029779460","https://openalex.org/W2029794817","https://openalex.org/W2031215694","https://openalex.org/W2036507354","https://openalex.org/W2037161130","https://openalex.org/W2037506305","https://openalex.org/W2043619056","https://openalex.org/W2044962560","https://openalex.org/W2045514727","https://openalex.org/W2045589999","https://openalex.org/W2046411745","https://openalex.org/W2053328000","https://openalex.org/W2054053393","https://openalex.org/W2054869657","https://openalex.org/W2055050070","https://openalex.org/W2056525187","https://openalex.org/W2057774703","https://openalex.org/W2063659650","https://openalex.org/W2067509872","https://openalex.org/W2068683917","https://openalex.org/W2069141366","https://openalex.org/W2069690875","https://openalex.org/W2070783701","https://openalex.org/W2080596545","https://openalex.org/W2083288715","https://openalex.org/W2083405070","https://openalex.org/W2084156728","https://openalex.org/W2096795069","https://openalex.org/W2098875323","https://openalex.org/W2109211487","https://openalex.org/W2116716969","https://openalex.org/W2118864235","https://openalex.org/W2120938951","https://openalex.org/W2124610014","https://openalex.org/W2128901141","https://openalex.org/W2132905138","https://openalex.org/W2142401829","https://openalex.org/W2146731001","https://openalex.org/W2155444369","https://openalex.org/W2160822610","https://openalex.org/W2165360511","https://openalex.org/W2177731497","https://openalex.org/W2345115707","https://openalex.org/W4241292222","https://openalex.org/W4242320431"],"related_works":["https://openalex.org/W3102746904","https://openalex.org/W2529051063","https://openalex.org/W2092147180","https://openalex.org/W2162651138","https://openalex.org/W2615024349","https://openalex.org/W4403741279","https://openalex.org/W2313496101","https://openalex.org/W2029522676","https://openalex.org/W2077215811","https://openalex.org/W4224278874"],"abstract_inverted_index":{"Examining":[0],"solute-induced":[1,27,230],"changes":[2,40,179,231],"in":[3,17,41,180,232],"protein":[4,19,218],"conformational":[5,233],"equilibria":[6],"is":[7,210],"a":[8,101],"long-standing":[9],"method":[10],"for":[11,38,117],"probing":[12],"the":[13,22,26,42,45,77,84,97,105,110,114,118,141,151,161,174,192,199,202,221],"role":[14],"of":[15,44,50,57,83,191,201,207,214,229],"water":[16],"maintaining":[18],"stability.":[20],"Interpreting":[21],"molecular":[23],"details":[24],"governing":[25],"effects,":[28],"however,":[29],"remains":[30],"controversial.":[31],"We":[32,133,167],"present":[33],"experimental":[34,165],"and":[35,47,72,86,129,148,155,182,195,223],"theoretical":[36],"data":[37,215],"osmolyte-induced":[39,217],"stabilities":[43,82],"A":[46,85,98,193],"N":[48,73,87,106,203],"states":[49,88],"yeast":[51],"iso-1-ferricytochrome":[52],"c.":[53],"Using":[54],"polyol":[55],"osmolytes":[56,63,95],"increasing":[58],"size,":[59],"we":[60,112,149],"observe":[61],"that":[62,169],"alone":[64],"induce":[65],"A-state":[66],"formation":[67,75],"from":[68,76,122,130,145,153,220],"acid-denatured":[69],"cytochrome":[70],"c":[71],"state":[74,99,194],"thermally":[78],"denatured":[79],"protein.":[80],"The":[81,205],"increase":[89],"linearly":[90],"with":[91,178,226],"osmolyte":[92],"concentration.":[93],"Interestingly,":[94],"stabilize":[96],"to":[100,139,164,189,198],"greater":[102],"degree":[103],"than":[104],"state.":[107,204],"To":[108],"interpret":[109],"data,":[111],"divide":[113],"free":[115,142],"energy":[116,143],"reaction":[119],"into":[120],"contributions":[121,144,152,188,197],"nonspecific":[123],"steric":[124,146],"repulsions":[125],"(excluded":[126],"volume":[127,171],"effects)":[128],"binding":[131,157,184],"interactions.":[132],"use":[134],"scaled":[135],"particle":[136],"theory":[137],"(SPT)":[138],"estimate":[140,150],"repulsions,":[147],"water-protein":[154,181],"osmolyte-protein":[156],"interactions":[158,185],"by":[159,212,224],"comparing":[160],"SPT":[162],"calculations":[163],"data.":[166],"conclude":[168],"excluded":[170],"effects":[172],"are":[173],"primary":[175],"stabilizing":[176],"force,":[177],"solute-protein":[183],"making":[186],"favorable":[187],"stability":[190,200],"unfavorable":[196],"validity":[206],"our":[208],"interpretation":[209],"strengthened":[211],"analysis":[213],"on":[216],"stabilization":[219],"literature,":[222],"comparison":[225],"other":[227],"analyses":[228],"equilibria.":[234]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":7},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":9}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}