{"id":"https://openalex.org/W2002185589","doi":"https://doi.org/10.1128/jvi.78.24.13708-13716.2004","title":"Identification of Residues in the Dengue Virus Type 2 NS2B Cofactor That Are Critical for NS3 Protease Activation","display_name":"Identification of Residues in the Dengue Virus Type 2 NS2B Cofactor That Are Critical for NS3 Protease Activation","publication_year":2004,"publication_date":"2004-11-24","ids":{"openalex":"https://openalex.org/W2002185589","doi":"https://doi.org/10.1128/jvi.78.24.13708-13716.2004","mag":"2002185589","pmid":"https://pubmed.ncbi.nlm.nih.gov/15564480","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/533897"},"language":"en","primary_location":{"id":"doi:10.1128/jvi.78.24.13708-13716.2004","doi":"https://doi.org/10.1128/jvi.78.24.13708-13716.2004","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.78.24.13708-13716.2004","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC533897","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056897035","display_name":"Pornwaratt Niyomrattanakit","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25399158","display_name":"Mahidol University","ror":"https://ror.org/01znkr924","country_code":"TH","type":"education","lineage":["https://openalex.org/I25399158"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Pornwaratt Niyomrattanakit","raw_affiliation_strings":["Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand","Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Phutthamonthon 4 Rd., Nakornpathom 73170, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand","institution_ids":["https://openalex.org/I25399158"]},{"raw_affiliation_string":"Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Phutthamonthon 4 Rd., Nakornpathom 73170, Thailand","institution_ids":["https://openalex.org/I25399158"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064300122","display_name":"Pakorn Winoyanuwattikun","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25399158","display_name":"Mahidol University","ror":"https://ror.org/01znkr924","country_code":"TH","type":"education","lineage":["https://openalex.org/I25399158"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Pakorn Winoyanuwattikun","raw_affiliation_strings":["Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand","institution_ids":["https://openalex.org/I25399158"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083925458","display_name":"Santad Chanprapaph","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25399158","display_name":"Mahidol University","ror":"https://ror.org/01znkr924","country_code":"TH","type":"education","lineage":["https://openalex.org/I25399158"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Santad Chanprapaph","raw_affiliation_strings":["Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand","institution_ids":["https://openalex.org/I25399158"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047765846","display_name":"Chanan Angsuthanasombat","orcid":"https://orcid.org/0000-0001-7891-9007","observed_orcids":["https://orcid.org/0000-0001-7891-9007"]},"institutions":[{"id":"https://openalex.org/I25399158","display_name":"Mahidol University","ror":"https://ror.org/01znkr924","country_code":"TH","type":"education","lineage":["https://openalex.org/I25399158"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Chanan Angsuthanasombat","raw_affiliation_strings":["Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand","institution_ids":["https://openalex.org/I25399158"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036381505","display_name":"Sakol Panyim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25399158","display_name":"Mahidol University","ror":"https://ror.org/01znkr924","country_code":"TH","type":"education","lineage":["https://openalex.org/I25399158"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Sakol Panyim","raw_affiliation_strings":["Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand","institution_ids":["https://openalex.org/I25399158"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030649539","display_name":"Gerd Katzenmeier","orcid":"https://orcid.org/0000-0002-8229-3710","observed_orcids":["https://orcid.org/0000-0002-8229-3710"]},"institutions":[{"id":"https://openalex.org/I25399158","display_name":"Mahidol University","ror":"https://ror.org/01znkr924","country_code":"TH","type":"education","lineage":["https://openalex.org/I25399158"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Gerd Katzenmeier","raw_affiliation_strings":["Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Nakornpathom, Thailand","institution_ids":["https://openalex.org/I25399158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I25399158"],"apc_list":null,"apc_paid":null,"fwci":5.7384,"has_fulltext":false,"cited_by_count":114,"citation_normalized_percentile":{"value":0.95543924,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"78","issue":"24","first_page":"13708","last_page":"13716"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dengue-virus-type-2","display_name":"dengue virus type 2","score":0.996999979019165},{"id":"https://openalex.org/keywords/ns3-serine-protease","display_name":"NS3 serine protease","score":0.9440000057220459},{"id":"https://openalex.org/keywords/protease-activation","display_name":"protease activation","score":0.8489999771118164},{"id":"https://openalex.org/keywords/autoproteolytic-cleavage","display_name":"autoproteolytic cleavage","score":0.8759999871253967},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.843999981880188},{"id":"https://openalex.org/keywords/antiviral-drug-targets","display_name":"antiviral drug targets","score":0.7020000219345093},{"id":"https://openalex.org/keywords/polyprotein-processing","display_name":"polyprotein processing","score":0.6660000085830688}],"concepts":[{"id":"https://openalex.org/C132379496","wikidata":"https://www.wikidata.org/wiki/Q83141645","display_name":"NS3","level":4,"score":0.9518340229988098},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.8019571900367737},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7143714427947998},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6270865201950073},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5540828704833984},{"id":"https://openalex.org/C83100098","wikidata":"https://www.wikidata.org/wiki/Q106297070","display_name":"NS2-3 protease","level":4,"score":0.538870632648468},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.5342527627944946},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.4661475419998169},{"id":"https://openalex.org/C82553315","wikidata":"https://www.wikidata.org/wiki/Q14905312","display_name":"MASP1","level":5,"score":0.4439130425453186},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.44016560912132263},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.38864272832870483},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.2973976135253906},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000085242","descriptor_name":"Viral Proteases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000409","descriptor_name":"Alanine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003716","descriptor_name":"Dengue Virus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D003716","descriptor_name":"Dengue Virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003716","descriptor_name":"Dengue Virus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017361","descriptor_name":"Viral Nonstructural Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017361","descriptor_name":"Viral Nonstructural Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017361","descriptor_name":"Viral Nonstructural Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D020365","descriptor_name":"RNA Helicases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D043583","descriptor_name":"Nucleoside-Triphosphatase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053487","descriptor_name":"DEAD-box RNA Helicases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/jvi.78.24.13708-13716.2004","doi":"https://doi.org/10.1128/jvi.78.24.13708-13716.2004","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.78.24.13708-13716.2004","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:142397","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC533897","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:15564480","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15564480","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:533897","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC533897/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:142397","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC533897","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322614","display_name":"Thailand Research Fund","ror":"https://ror.org/03gd1bh37"},{"id":"https://openalex.org/F4320327548","display_name":"Royal Golden Jubilee (RGJ) Ph.D. Programme","ror":"https://ror.org/05fp08r12"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":52,"referenced_works":["https://openalex.org/W103443620","https://openalex.org/W1564935170","https://openalex.org/W1570836961","https://openalex.org/W1579199588","https://openalex.org/W1582754056","https://openalex.org/W1595419937","https://openalex.org/W1622437624","https://openalex.org/W1793827589","https://openalex.org/W1826433850","https://openalex.org/W1865324159","https://openalex.org/W1933943651","https://openalex.org/W1975085160","https://openalex.org/W1978035722","https://openalex.org/W1991352994","https://openalex.org/W2012172587","https://openalex.org/W2012761715","https://openalex.org/W2018750823","https://openalex.org/W2025638022","https://openalex.org/W2030439521","https://openalex.org/W2032676385","https://openalex.org/W2040552041","https://openalex.org/W2045211613","https://openalex.org/W2057065474","https://openalex.org/W2060148547","https://openalex.org/W2062725625","https://openalex.org/W2064230725","https://openalex.org/W2065283382","https://openalex.org/W2072083315","https://openalex.org/W2074188269","https://openalex.org/W2075582324","https://openalex.org/W2078463580","https://openalex.org/W2078798933","https://openalex.org/W2079949399","https://openalex.org/W2091937293","https://openalex.org/W2099955730","https://openalex.org/W2113638843","https://openalex.org/W2117933797","https://openalex.org/W2123114899","https://openalex.org/W2124000798","https://openalex.org/W2125525079","https://openalex.org/W2131112023","https://openalex.org/W2136446600","https://openalex.org/W2142821747","https://openalex.org/W2145469333","https://openalex.org/W2149459759","https://openalex.org/W2149590832","https://openalex.org/W2150029541","https://openalex.org/W2155606687","https://openalex.org/W2156280454","https://openalex.org/W2163412375","https://openalex.org/W2166556525","https://openalex.org/W2184478842"],"related_works":["https://openalex.org/W2145704611","https://openalex.org/W2065758508","https://openalex.org/W4200428449","https://openalex.org/W1968207680","https://openalex.org/W2560700961","https://openalex.org/W2079949399","https://openalex.org/W1793827589","https://openalex.org/W2030439521","https://openalex.org/W2181356980","https://openalex.org/W2471532407"],"abstract_inverted_index":{"Proteolytic":[0],"processing":[1],"of":[2,29,35,50,71,117,123,138,148,188,204,222,237],"the":[3,14,27,36,44,51,54,72,86,121,124,142,149,152,159,162,173,176,189,198,205,230,234,238],"dengue":[4,239],"virus":[5,240],"polyprotein":[6],"is":[7],"mediated":[8],"by":[9,53,89,131,146],"host":[10],"cell":[11],"proteases":[12],"and":[13,98,106,145,165,175,227],"virus-encoded":[15],"NS2B-NS3":[16],"two-component":[17],"protease.":[18,243],"The":[19,32,115],"NS3":[20,37,78,241],"protease":[21,38,79,126],"represents":[22],"an":[23,109],"attractive":[24],"target":[25],"for":[26,48,197],"development":[28],"antiviral":[30],"inhibitors.":[31],"three-dimensional":[33],"structure":[34],"domain":[39],"has":[40],"been":[41,58],"determined,":[42],"but":[43],"structural":[45,235],"determinants":[46],"necessary":[47],"activation":[49,236],"enzyme":[52,150],"NS2B":[55,87,101,231],"cofactor":[56,88,232],"have":[57],"characterized":[59],"only":[60],"to":[61,158,181,202],"a":[62,67,194,219],"limited":[63],"extent.":[64],"To":[65],"test":[66],"possible":[68],"functional":[69],"role":[70],"recently":[73],"proposed":[74],"Phix(3)Phi":[75],"motif":[76],"in":[77,100,107,129,172,229,233],"activation,":[80],"we":[81],"targeted":[82],"six":[83],"residues":[84,224],"within":[85],"site-specific":[90],"mutagenesis.":[91],"Residues":[92],"Trp62,":[93,225],"Ser71,":[94],"Leu75,":[95,226],"Ile77,":[96],"Thr78,":[97],"Ile79":[99,228],"were":[102,127],"replaced":[103],"with":[104,151],"alanine,":[105],"addition,":[108],"L75A/I79A":[110,177],"double":[111],"mutant":[112],"was":[113],"generated.":[114],"effects":[116],"these":[118],"mutations":[119],"on":[120],"activity":[122],"NS2B(H)-NS3pro":[125],"analyzed":[128],"vitro":[130],"sodium":[132],"dodecyl":[133],"sulfate-polyacrylamide":[134],"gel":[135],"electrophoresis":[136],"analysis":[137],"autoproteolytic":[139],"cleavage":[140],"at":[141],"NS2B/NS3":[143],"site":[144],"assay":[147],"fluorogenic":[153],"peptide":[154,200],"substrate":[155],"GRR-AMC.":[156],"Compared":[157],"wild":[160,206],"type,":[161,207],"L75A,":[163],"I77A,":[164],"I79A":[166],"mutants":[167,178,210],"demonstrated":[168],"inefficient":[169],"autoproteolysis,":[170],"whereas":[171],"W62A":[174],"self-cleavage":[179],"appeared":[180],"be":[182],"almost":[183],"completely":[184],"abolished.":[185],"With":[186],"exception":[187],"S71A":[190],"mutant,":[191],"which":[192],"had":[193],"k(cat)/K(m)":[195],"value":[196],"GRR-AMC":[199],"similar":[201],"that":[203],"all":[208],"other":[209],"exhibited":[211],"drastically":[212],"reduced":[213],"k(cat)":[214],"values.":[215],"These":[216],"results":[217],"indicate":[218],"pivotal":[220],"function":[221],"conserved":[223],"serine":[242]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":9},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":8},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":9},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}
