{"id":"https://openalex.org/W2002172128","doi":"https://doi.org/10.1002/bit.260280912","title":"Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells","display_name":"Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells","publication_year":1986,"publication_date":"1986-09-01","ids":{"openalex":"https://openalex.org/W2002172128","doi":"https://doi.org/10.1002/bit.260280912","mag":"2002172128","pmid":"https://pubmed.ncbi.nlm.nih.gov/18561227"},"language":"en","primary_location":{"id":"doi:10.1002/bit.260280912","doi":"https://doi.org/10.1002/bit.260280912","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260280912","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040158970","display_name":"Michael W. Glacken","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. W. Glacken","raw_affiliation_strings":["Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084523215","display_name":"Robert J. Fleischaker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. J. Fleischaker","raw_affiliation_strings":["Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5042953895","display_name":"Anthony J. Sinskey","orcid":"https://orcid.org/0000-0002-1015-1270","observed_orcids":["https://orcid.org/0000-0002-1015-1270","https://orcid.org/0000-0001-9433-4324"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A. J. Sinskey","raw_affiliation_strings":["Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139","institution_ids":["https://openalex.org/I63966007"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":2.3262,"has_fulltext":false,"cited_by_count":354,"citation_normalized_percentile":{"value":0.89359886,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"28","issue":"9","first_page":"1376","last_page":"1389"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10864","display_name":"Fluid Dynamics and Mixing","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/1507","display_name":"Fluid Flow and Transfer Processes"},"field":{"id":"https://openalex.org/fields/15","display_name":"Chemical Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mammalian-cell-culture","display_name":"mammalian cell culture","score":0.8930000066757202},{"id":"https://openalex.org/keywords/lactic-acid","display_name":"lactic acid","score":0.8679999709129333},{"id":"https://openalex.org/keywords/ammonium","display_name":"ammonium","score":0.8149999976158142},{"id":"https://openalex.org/keywords/glycemic-control","display_name":"glycemic control","score":0.5879999995231628},{"id":"https://openalex.org/keywords/oxygen-uptake-rate","display_name":"oxygen uptake rate","score":0.9319999814033508},{"id":"https://openalex.org/keywords/product-yield","display_name":"product yield","score":0.6729999780654907},{"id":"https://openalex.org/keywords/process-control","display_name":"process control","score":0.5509999990463257}],"concepts":[{"id":"https://openalex.org/C2779349466","wikidata":"https://www.wikidata.org/wiki/Q181619","display_name":"Glutamine","level":3,"score":0.6864010095596313},{"id":"https://openalex.org/C2775920511","wikidata":"https://www.wikidata.org/wiki/Q161249","display_name":"Lactic acid","level":3,"score":0.5865908265113831},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.5844728946685791},{"id":"https://openalex.org/C2779870022","wikidata":"https://www.wikidata.org/wiki/Q190901","display_name":"Ammonium","level":2,"score":0.5729433298110962},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.56829833984375},{"id":"https://openalex.org/C2780434155","wikidata":"https://www.wikidata.org/wiki/Q7247767","display_name":"Product inhibition","level":4,"score":0.5438840985298157},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5172048807144165},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4890924096107483},{"id":"https://openalex.org/C10146269","wikidata":"https://www.wikidata.org/wiki/Q185557","display_name":"Excretion","level":2,"score":0.48026061058044434},{"id":"https://openalex.org/C206139338","wikidata":"https://www.wikidata.org/wiki/Q192355","display_name":"Raw material","level":2,"score":0.4538976848125458},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.3427874445915222},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2864171266555786},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.19673967361450195},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.17779207229614258},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.12883919477462769},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.11188119649887085},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1028738021850586},{"id":"https://openalex.org/C150757390","wikidata":"https://www.wikidata.org/wiki/Q1985205","display_name":"Non-competitive inhibition","level":3,"score":0.0},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1002/bit.260280912","doi":"https://doi.org/10.1002/bit.260280912","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260280912","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:18561227","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18561227","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W608432245","https://openalex.org/W1483212110","https://openalex.org/W1491802973","https://openalex.org/W1615014443","https://openalex.org/W1978212802","https://openalex.org/W1987958868","https://openalex.org/W1999866286","https://openalex.org/W2004314923","https://openalex.org/W2011885754","https://openalex.org/W2044553926","https://openalex.org/W2061313324","https://openalex.org/W2072174634","https://openalex.org/W2080712703","https://openalex.org/W2094958788","https://openalex.org/W2121829896","https://openalex.org/W2141172423","https://openalex.org/W2330686900","https://openalex.org/W2332584636","https://openalex.org/W2334439047","https://openalex.org/W2478299533","https://openalex.org/W3215619572"],"related_works":["https://openalex.org/W2018727351","https://openalex.org/W2214113939","https://openalex.org/W1270599943","https://openalex.org/W2041746790","https://openalex.org/W195474552","https://openalex.org/W2792637043","https://openalex.org/W1898008112","https://openalex.org/W2052355054","https://openalex.org/W2093999909","https://openalex.org/W1529091265"],"abstract_inverted_index":{"Mammalian":[0],"cells":[1,85],"grown":[2],"in":[3,11,45,150],"culture":[4,25],"excrete":[5],"lactic":[6,80,134],"acid":[7,81,135],"and":[8,17,64,69,82,91,100,132],"ammonium":[9,83,99],"ions":[10],"quantities":[12],"that":[13],"may":[14],"limit":[15],"growth":[16],"reduce":[18],"product":[19,33,65],"synthesis.":[20],"Frequent":[21],"replenishment":[22,43],"of":[23,48,58,79,89,113,141],"the":[24,51,62,77,114,123,128,133,142],"medium":[26,42],"is":[27,145],"often":[28],"necessary":[29],"to":[30,155],"prevent":[31],"waste":[32,59],"accumulation":[34],"which":[35],"could":[36],"inhibit":[37],"cell":[38,63,115,143],"growth.":[39],"Since":[40],"increased":[41,46],"results":[44],"usage":[47],"animal":[49],"serum,":[50,68],"most":[52],"expensive":[53],"raw":[54],"material,":[55],"excessive":[56],"production":[57,72,78,125,136],"products":[60],"lowers":[61],"yield":[66],"on":[67],"hence":[70],"increases":[71],"costs.":[73],"Strategies":[74],"for":[75,109],"reducing":[76],"bymammalian":[84],"via":[86,159],"controlled":[87],"addition":[88],"glucose":[90],"glutamine":[92,101],"will":[93,103],"be":[94,104,156],"demonstrated.":[95],"Mathematical":[96],"relations":[97],"coupling":[98],"kinetics":[102],"described.":[105],"Additionally,":[106],"a":[107],"method":[108,120],"automatic":[110],"on-line":[111],"estimation":[112,140],"concentration":[116,144],"was":[117],"developed.":[118],"This":[119],"involves":[121],"calculating":[122],"ATP":[124],"rate":[126,131],"from":[127],"oxygen":[129],"uptake":[130],"rate.":[137],"Automatic":[138],"online":[139],"critical":[146],"if":[147],"nutrient":[148],"levels":[149],"large-scale":[151],"mammaliancell":[152],"cultures":[153],"are":[154],"accurately":[157],"maintained":[158],"process":[160],"control.":[161]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":10},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":9}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}