{"id":"https://openalex.org/W2002127274","doi":"https://doi.org/10.1128/aem.06382-11","title":"Metabolic Engineering of Clostridium acetobutylicum ATCC 824 for Isopropanol-Butanol-Ethanol Fermentation","display_name":"Metabolic Engineering of Clostridium acetobutylicum ATCC 824 for Isopropanol-Butanol-Ethanol Fermentation","publication_year":2011,"publication_date":"2011-12-31","ids":{"openalex":"https://openalex.org/W2002127274","doi":"https://doi.org/10.1128/aem.06382-11","mag":"2002127274","pmid":"https://pubmed.ncbi.nlm.nih.gov/22210214","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3294493"},"language":"en","primary_location":{"id":"doi:10.1128/aem.06382-11","doi":"https://doi.org/10.1128/aem.06382-11","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.06382-11","pdf_url":"https://aem.asm.org/content/aem/78/5/1416.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://aem.asm.org/content/aem/78/5/1416.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5062180454","display_name":"Joungmin Lee","orcid":"https://orcid.org/0000-0002-3722-3756","observed_orcids":["https://orcid.org/0000-0002-3722-3756"]},"institutions":[{"id":"https://openalex.org/I157485424","display_name":"Korea Advanced Institute of Science and Technology","ror":"https://ror.org/05apxxy63","country_code":"KR","type":"education","lineage":["https://openalex.org/I157485424"]},{"id":"https://openalex.org/I4210110126","display_name":"Intelligent Synthetic Biology Center","ror":"https://ror.org/023dqar74","country_code":"KR","type":"other","lineage":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Joungmin Lee","raw_affiliation_strings":["Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea","institution_ids":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008435545","display_name":"Yu\u2010Sin Jang","orcid":"https://orcid.org/0000-0003-2044-0479","observed_orcids":["https://orcid.org/0000-0003-2044-0479"]},"institutions":[{"id":"https://openalex.org/I157485424","display_name":"Korea Advanced Institute of Science and Technology","ror":"https://ror.org/05apxxy63","country_code":"KR","type":"education","lineage":["https://openalex.org/I157485424"]},{"id":"https://openalex.org/I4210110126","display_name":"Intelligent Synthetic Biology Center","ror":"https://ror.org/023dqar74","country_code":"KR","type":"other","lineage":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Yu-Sin Jang","raw_affiliation_strings":["BioFuelChem, Daejeon, Republic of Korea","Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BioFuelChem, Daejeon, Republic of Korea","institution_ids":[]},{"raw_affiliation_string":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea","institution_ids":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109419959","display_name":"Sung Jun Choi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157485424","display_name":"Korea Advanced Institute of Science and Technology","ror":"https://ror.org/05apxxy63","country_code":"KR","type":"education","lineage":["https://openalex.org/I157485424"]},{"id":"https://openalex.org/I4210110126","display_name":"Intelligent Synthetic Biology Center","ror":"https://ror.org/023dqar74","country_code":"KR","type":"other","lineage":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Sung Jun Choi","raw_affiliation_strings":["Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea","institution_ids":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042061973","display_name":"Jung Ae Im","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157485424","display_name":"Korea Advanced Institute of Science and Technology","ror":"https://ror.org/05apxxy63","country_code":"KR","type":"education","lineage":["https://openalex.org/I157485424"]},{"id":"https://openalex.org/I4210110126","display_name":"Intelligent Synthetic Biology Center","ror":"https://ror.org/023dqar74","country_code":"KR","type":"other","lineage":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jung Ae Im","raw_affiliation_strings":["Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea","institution_ids":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102800927","display_name":"Hyohak Song","orcid":"https://orcid.org/0000-0001-7376-6355","observed_orcids":["https://orcid.org/0000-0001-7376-6355"]},"institutions":[{"id":"https://openalex.org/I4210089444","display_name":"GS Caltex (South Korea)","ror":"https://ror.org/00bvkj141","country_code":"KR","type":"company","lineage":["https://openalex.org/I4210089444"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Hyohak Song","raw_affiliation_strings":["GS Caltex Corporation Value Creation Center, Daejeon, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GS Caltex Corporation Value Creation Center, Daejeon, Republic of Korea","institution_ids":["https://openalex.org/I4210089444"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050237530","display_name":"Jung Hee Cho","orcid":"https://orcid.org/0000-0002-6147-7473","observed_orcids":["https://orcid.org/0000-0002-6147-7473"]},"institutions":[{"id":"https://openalex.org/I4210089444","display_name":"GS Caltex (South Korea)","ror":"https://ror.org/00bvkj141","country_code":"KR","type":"company","lineage":["https://openalex.org/I4210089444"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jung Hee Cho","raw_affiliation_strings":["GS Caltex Corporation Value Creation Center, Daejeon, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GS Caltex Corporation Value Creation Center, Daejeon, Republic of Korea","institution_ids":["https://openalex.org/I4210089444"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019324918","display_name":"Do Young Seung","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210089444","display_name":"GS Caltex (South Korea)","ror":"https://ror.org/00bvkj141","country_code":"KR","type":"company","lineage":["https://openalex.org/I4210089444"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Do Young Seung","raw_affiliation_strings":["GS Caltex Corporation Value Creation Center, Daejeon, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GS Caltex Corporation Value Creation Center, Daejeon, Republic of Korea","institution_ids":["https://openalex.org/I4210089444"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112775515","display_name":"Eleftherios Terry Papoutsakis","orcid":"https://orcid.org/0000-0002-1077-1277","observed_orcids":["https://orcid.org/0000-0002-1077-1277"]},"institutions":[{"id":"https://openalex.org/I4405261003","display_name":"Delaware Biotechnology Institute","ror":"https://ror.org/04m1ydt32","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405261003","https://openalex.org/I86501945"]},{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"E. Terry Papoutsakis","raw_affiliation_strings":["Department of Chemical Engineering and Delaware Biotechnology Institute, University of Delaware, Newark, Deleware, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering and Delaware Biotechnology Institute, University of Delaware, Newark, Deleware, USA","institution_ids":["https://openalex.org/I4405261003","https://openalex.org/I86501945"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038858646","display_name":"George N. Bennett","orcid":"https://orcid.org/0000-0003-1408-8915","observed_orcids":["https://orcid.org/0000-0003-1408-8915"]},"institutions":[{"id":"https://openalex.org/I74775410","display_name":"Rice University","ror":"https://ror.org/008zs3103","country_code":"US","type":"education","lineage":["https://openalex.org/I74775410"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"George N. Bennett","raw_affiliation_strings":["Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA","institution_ids":["https://openalex.org/I74775410"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100616675","display_name":"Sang Yup Lee","orcid":"https://orcid.org/0000-0003-0599-3091","observed_orcids":["https://orcid.org/0000-0003-0599-3091"]},"institutions":[{"id":"https://openalex.org/I157485424","display_name":"Korea Advanced Institute of Science and Technology","ror":"https://ror.org/05apxxy63","country_code":"KR","type":"education","lineage":["https://openalex.org/I157485424"]},{"id":"https://openalex.org/I4210110126","display_name":"Intelligent Synthetic Biology Center","ror":"https://ror.org/023dqar74","country_code":"KR","type":"other","lineage":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Sang Yup Lee","raw_affiliation_strings":["Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea","institution_ids":["https://openalex.org/I157485424","https://openalex.org/I4210110126"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":6,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":6.2057,"has_fulltext":false,"cited_by_count":248,"citation_normalized_percentile":{"value":0.97876085,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"78","issue":"5","first_page":"1416","last_page":"1423"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.00559999980032444,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T14150","display_name":"Biochemical and biochemical processes","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/clostridium-acetobutylicum","display_name":"Clostridium acetobutylicum","score":0.9919999837875366},{"id":"https://openalex.org/keywords/alcohol-dehydrogenase","display_name":"alcohol dehydrogenase","score":0.7919999957084656},{"id":"https://openalex.org/keywords/clostridium-beijerinckii","display_name":"Clostridium beijerinckii","score":0.8569999933242798},{"id":"https://openalex.org/keywords/butyrate-kinase","display_name":"butyrate kinase","score":0.6140000224113464},{"id":"https://openalex.org/keywords/biofuels","display_name":"biofuels","score":0.49799999594688416},{"id":"https://openalex.org/keywords/gas-stripping","display_name":"gas stripping","score":0.7080000042915344},{"id":"https://openalex.org/keywords/solvent-removal","display_name":"solvent removal","score":0.5199999809265137}],"concepts":[{"id":"https://openalex.org/C2776928978","wikidata":"https://www.wikidata.org/wiki/Q148692","display_name":"Clostridium acetobutylicum","level":4,"score":0.9809893369674683},{"id":"https://openalex.org/C2776645570","wikidata":"https://www.wikidata.org/wiki/Q3681018","display_name":"Clostridium beijerinckii","level":4,"score":0.8419616222381592},{"id":"https://openalex.org/C2777713963","wikidata":"https://www.wikidata.org/wiki/Q663902","display_name":"Butanol","level":3,"score":0.839533269405365},{"id":"https://openalex.org/C2781213060","wikidata":"https://www.wikidata.org/wiki/Q49546","display_name":"Acetone","level":2,"score":0.7415199279785156},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6914584636688232},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.6887955665588379},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.6521621942520142},{"id":"https://openalex.org/C2777344606","wikidata":"https://www.wikidata.org/wiki/Q410754","display_name":"Alcohol dehydrogenase","level":3,"score":0.6352500319480896},{"id":"https://openalex.org/C2780161600","wikidata":"https://www.wikidata.org/wiki/Q153","display_name":"Ethanol","level":2,"score":0.5549312233924866},{"id":"https://openalex.org/C2781092032","wikidata":"https://www.wikidata.org/wiki/Q327663","display_name":"Clostridium","level":3,"score":0.4851582646369934},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.46163439750671387},{"id":"https://openalex.org/C168170006","wikidata":"https://www.wikidata.org/wiki/Q864699","display_name":"Bioreactor","level":2,"score":0.45520734786987305},{"id":"https://openalex.org/C53991642","wikidata":"https://www.wikidata.org/wiki/Q128991","display_name":"Biofuel","level":2,"score":0.4324271082878113},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.18133440613746643},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.17548370361328125},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.13936135172843933},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1010991632938385},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.09555211663246155},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000096","descriptor_name":"Acetone","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000426","descriptor_name":"Alcohol Dehydrogenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000426","descriptor_name":"Alcohol Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000440","descriptor_name":"Butanols","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019840","descriptor_name":"2-Propanol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D046969","descriptor_name":"Clostridium acetobutylicum","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D046969","descriptor_name":"Clostridium acetobutylicum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D046969","descriptor_name":"Clostridium acetobutylicum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D046970","descriptor_name":"Clostridium beijerinckii","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D046970","descriptor_name":"Clostridium beijerinckii","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D056804","descriptor_name":"Biofuels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1128/aem.06382-11","doi":"https://doi.org/10.1128/aem.06382-11","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.06382-11","pdf_url":"https://aem.asm.org/content/aem/78/5/1416.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2431966","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3294493","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3294493","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3294493","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"},{"id":"pmid:22210214","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22210214","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1128/aem.06382-11","doi":"https://doi.org/10.1128/aem.06382-11","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.06382-11","pdf_url":"https://aem.asm.org/content/aem/78/5/1416.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8485000133514404,"id":"https://metadata.un.org/sdg/7","display_name":"Affordable and clean energy"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320323505","display_name":"Universidad de La Frontera","ror":"https://ror.org/04v0snf24"},{"id":"https://openalex.org/F4320324161","display_name":"Korea Advanced Institute of Science and Technology","ror":"https://ror.org/05apxxy63"}],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2002127274.pdf"},"referenced_works_count":46,"referenced_works":["https://openalex.org/W1529159985","https://openalex.org/W1580150127","https://openalex.org/W1603925270","https://openalex.org/W1786035617","https://openalex.org/W1813168975","https://openalex.org/W1939556909","https://openalex.org/W1963804065","https://openalex.org/W1964987246","https://openalex.org/W1968362149","https://openalex.org/W1968438822","https://openalex.org/W1995716143","https://openalex.org/W2005612744","https://openalex.org/W2009188183","https://openalex.org/W2010985753","https://openalex.org/W2012660430","https://openalex.org/W2016894508","https://openalex.org/W2026062081","https://openalex.org/W2044536618","https://openalex.org/W2044952215","https://openalex.org/W2061510626","https://openalex.org/W2071749681","https://openalex.org/W2078782582","https://openalex.org/W2089691795","https://openalex.org/W2098508815","https://openalex.org/W2101025278","https://openalex.org/W2105639755","https://openalex.org/W2106986625","https://openalex.org/W2110528780","https://openalex.org/W2116963720","https://openalex.org/W2128981014","https://openalex.org/W2131207988","https://openalex.org/W2131812615","https://openalex.org/W2134972743","https://openalex.org/W2136343510","https://openalex.org/W2141783351","https://openalex.org/W2144229671","https://openalex.org/W2150088285","https://openalex.org/W2152485488","https://openalex.org/W2152560831","https://openalex.org/W2160944601","https://openalex.org/W2163257220","https://openalex.org/W2168935258","https://openalex.org/W2321292365","https://openalex.org/W4232147926","https://openalex.org/W4246052834","https://openalex.org/W4300672472"],"related_works":["https://openalex.org/W2116154244","https://openalex.org/W2005815224","https://openalex.org/W645360052","https://openalex.org/W2138638690","https://openalex.org/W2788145516","https://openalex.org/W2396148777","https://openalex.org/W3028389229","https://openalex.org/W4392337274","https://openalex.org/W7143937485","https://openalex.org/W2789932790"],"abstract_inverted_index":{"Clostridium":[0,101],"acetobutylicum":[1,79,109],"naturally":[2],"produces":[3],"acetone":[4,12,42,52,120],"as":[5,7,16,63,84],"well":[6],"butanol":[8],"and":[9,72,127,158,188],"ethanol.":[10],"Since":[11,58],"cannot":[13],"be":[14,23,61,81,163,177,196],"used":[15,62,83],"a":[17,64,85,95,118,154],"biofuel,":[18],"its":[19],"production":[20,88],"needs":[21],"to":[22,37,53,129,142],"minimized":[24],"or":[25,29,39],"suppressed":[26],"by":[27,55,76,91,179,185],"cell":[28],"bioreactor":[30],"engineering.":[31,57],"Thus,":[32],"there":[33],"have":[34],"been":[35],"attempts":[36],"disrupt":[38],"inactivate":[40],"the":[41,67,92,114,131,143,148,192],"formation":[43],"pathway.":[44],"Here":[45],"we":[46],"present":[47],"another":[48],"approach,":[49],"namely,":[50],"converting":[51],"isopropanol":[54,59,134],"metabolic":[56],"can":[60,80],"fuel":[65],"additive,":[66],"mixture":[68,194],"of":[69,94,160,172,183,191],"isopropanol,":[70],"butanol,":[71],"ethanol":[73],"(IBE)":[74],"produced":[75,169,197],"engineered":[77],"C.":[78,108],"directly":[82],"biofuel.":[86],"IBE":[87,161,193],"is":[89],"achieved":[90],"expression":[93],"primary/secondary":[96],"alcohol":[97,116],"dehydrogenase":[98],"gene":[99,150],"from":[100],"beijerinckii":[102],"NRRL":[103],"B-593":[104],"(i.e.,":[105],"adh(B-593))":[106],"in":[107,147,180,198],"ATCC":[110],"824.":[111],"To":[112],"increase":[113,130],"total":[115,173],"titer,":[117],"synthetic":[119],"operon":[121],"(act":[122],"operon;":[123],"adc-ctfA-ctfB)":[124],"was":[125,140],"constructed":[126],"expressed":[128],"flux":[132],"toward":[133],"formation.":[135],"When":[136],"this":[137],"engineering":[138],"strategy":[139],"applied":[141],"PJC4BK":[144],"strain":[145,168],"lacking":[146],"buk":[149],"(encoding":[151],"butyrate":[152],"kinase),":[153],"significantly":[155],"higher":[156],"titer":[157],"yield":[159],"could":[162,176,195],"achieved.":[164],"The":[165],"resulting":[166],"PJC4BK(pIPA3-Cm2)":[167],"20.4":[170],"g/liter":[171,190],"alcohol.":[174],"Fermentation":[175],"prolonged":[178],"situ":[181],"removal":[182],"solvents":[184],"gas":[186],"stripping,":[187],"35.6":[189],"45":[199],"h.":[200]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":9},{"year":2022,"cited_by_count":17},{"year":2021,"cited_by_count":13},{"year":2020,"cited_by_count":16},{"year":2019,"cited_by_count":22},{"year":2018,"cited_by_count":21},{"year":2017,"cited_by_count":29},{"year":2016,"cited_by_count":27},{"year":2015,"cited_by_count":21},{"year":2014,"cited_by_count":27},{"year":2013,"cited_by_count":18},{"year":2012,"cited_by_count":13}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}