{"id":"https://openalex.org/W2002064053","doi":"https://doi.org/10.1021/bp970138u","title":"Production and Purification of Two Recombinant Proteins from Transgenic Corn","display_name":"Production and Purification of Two Recombinant Proteins from Transgenic Corn","publication_year":1998,"publication_date":"1998-02-06","ids":{"openalex":"https://openalex.org/W2002064053","doi":"https://doi.org/10.1021/bp970138u","mag":"2002064053","pmid":"https://pubmed.ncbi.nlm.nih.gov/9496680"},"language":"en","primary_location":{"id":"doi:10.1021/bp970138u","doi":"https://doi.org/10.1021/bp970138u","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bp970138u","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039936196","display_name":"Ann R. Kusnadi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A.R. Kusnadi","raw_affiliation_strings":["Department of Food Science and Human Nutrition, Iowa State University, Ames 50011, USA","Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Human Nutrition, Iowa State University, Ames 50011, USA","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011","institution_ids":["https://openalex.org/I173911158"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071742925","display_name":"Elizabeth E. Hood","orcid":"https://orcid.org/0000-0001-8517-2618","observed_orcids":["https://orcid.org/0000-0001-8517-2618"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E.E. Hood","raw_affiliation_strings":["ProdiGene, Inc., College Station, Texas 77845"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ProdiGene, Inc., College Station, Texas 77845","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062912509","display_name":"Derrick R. Witcher","orcid":"https://orcid.org/0000-0002-2248-0143","observed_orcids":["https://orcid.org/0000-0002-2248-0143"]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D.R. Witcher","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Johnston, Iowa 50131"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Johnston, Iowa 50131","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003338788","display_name":"John A. Howard","orcid":"https://orcid.org/0000-0002-5974-5416","observed_orcids":["https://orcid.org/0000-0002-5974-5416"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J.A. Howard","raw_affiliation_strings":["ProdiGene, Inc., College Station, Texas 77845"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ProdiGene, Inc., College Station, Texas 77845","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000145435","display_name":"Z\u0306ivko L. Nikolov","orcid":"https://orcid.org/0000-0002-4000-9697","observed_orcids":["https://orcid.org/0000-0002-4000-9697"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Z.L. Nikolov","raw_affiliation_strings":["Center for Crops Utilization Research, Iowa State University, Ames, Iowa 50011","Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa 50011","Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Crops Utilization Research, Iowa State University, Ames, Iowa 50011","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa 50011","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011","institution_ids":["https://openalex.org/I173911158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5000145435"],"corresponding_institution_ids":["https://openalex.org/I173911158"],"apc_list":{"value":4160,"currency":"USD","value_usd":4160},"apc_paid":null,"fwci":6.5415,"has_fulltext":false,"cited_by_count":143,"citation_normalized_percentile":{"value":0.98035067,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"14","issue":"1","first_page":"149","last_page":"155"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transgenic-maize","display_name":"transgenic maize","score":0.8799999952316284},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8759999871253967},{"id":"https://openalex.org/keywords/beta-glucuronidase","display_name":"beta-glucuronidase","score":0.9070000052452087},{"id":"https://openalex.org/keywords/avidin","display_name":"avidin","score":0.9869999885559082},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.6629999876022339},{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.503000020980835},{"id":"https://openalex.org/keywords/storage-stability","display_name":"storage stability","score":0.5910000205039978},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.7730000019073486},{"id":"https://openalex.org/keywords/ubiquitin-promoter","display_name":"ubiquitin promoter","score":0.7200000286102295},{"id":"https://openalex.org/keywords/ion-exchange-chromatography","display_name":"ion-exchange chromatography","score":0.7820000052452087},{"id":"https://openalex.org/keywords/hydrophobic-interaction-chromatography","display_name":"hydrophobic interaction chromatography","score":0.9800000190734863},{"id":"https://openalex.org/keywords/size-exclusion-chromatography","display_name":"size exclusion chromatography","score":0.9409999847412109},{"id":"https://openalex.org/keywords/protein-extraction","display_name":"protein extraction","score":0.3490000069141388}],"concepts":[{"id":"https://openalex.org/C176406525","wikidata":"https://www.wikidata.org/wiki/Q24775166","display_name":"Avidin","level":3,"score":0.8969500660896301},{"id":"https://openalex.org/C154615088","wikidata":"https://www.wikidata.org/wiki/Q1363424","display_name":"Genetically modified maize","level":5,"score":0.6022809147834778},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5961312651634216},{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.5484333038330078},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44103601574897766},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4334263503551483},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.42780381441116333},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.37437739968299866},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.35372182726860046},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.34602105617523193},{"id":"https://openalex.org/C204628709","wikidata":"https://www.wikidata.org/wiki/Q864854","display_name":"Biotinylation","level":2,"score":0.22859176993370056},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1345280110836029}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001360","descriptor_name":"Avidin","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D001360","descriptor_name":"Avidin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003313","descriptor_name":"Zea mays","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004355","descriptor_name":"Drug Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005966","descriptor_name":"Glucuronidase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D005966","descriptor_name":"Glucuronidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bp970138u","doi":"https://doi.org/10.1021/bp970138u","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bp970138u","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},{"id":"pmid:9496680","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9496680","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology progress","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.9208999872207642}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W207232023","https://openalex.org/W348514121","https://openalex.org/W2029101274","https://openalex.org/W3171286318","https://openalex.org/W2007981797","https://openalex.org/W2026865316","https://openalex.org/W1972897935","https://openalex.org/W2030785044","https://openalex.org/W7097693592","https://openalex.org/W2016366290"],"abstract_inverted_index":{"This":[0],"study":[1],"reports":[2],"the":[3,31,57,67,74,126,148,196],"production,":[4],"purification,":[5],"and":[6,14,24,33,43,50,60,101,123,153,181,189],"characterization":[7],"of":[8,41,52,73,115,151,176,186,195],"recombinant":[9],"Escherichia":[10],"coli":[11],"beta-glucuronidase":[12],"(GUS)":[13],"chicken":[15],"egg-white":[16],"avidin":[17,23,42,59,88,124,152,188],"from":[18,155],"transgenic":[19,83],"corn":[20,46],"seed.":[21],"The":[22,38,77,117,140],"gus":[25],"genes":[26],"were":[27,48,129,191],"stably":[28],"integrated":[29],"in":[30,45,125,161,172],"genome":[32],"expressed":[34],"over":[35],"seven":[36],"generations.":[37],"accumulation":[39,62],"levels":[40],"GUS":[44,61,86,122,154,169,190],"kernel":[47],"5.7%":[49],"0.7%":[51],"extractable":[53],"protein,":[54],"respectively.":[55],"Within":[56],"kernel,":[58],"was":[63,159,170],"mainly":[64],"localized":[65],"to":[66,108,137,193],"germ,":[68],"indicating":[69],"possible":[70],"tissue":[71],"preference":[72],"ubiquitin":[75],"promoter.":[76],"storage-stability":[78],"studies":[79],"demonstrated":[80],"that":[81,121],"processed":[82],"seed":[84],"containing":[85],"or":[87],"can":[89],"be":[90],"stored":[91],"at":[92,97,102,131],"10":[93],"degrees":[94,104,133],"C":[95,105,134],"for":[96,106,135],"least":[98],"3":[99],"months":[100],"25":[103],"up":[107,136],"2":[109],"weeks":[110],"without":[111],"a":[112],"significant":[113],"loss":[114],"activity.":[116],"heat-stability":[118],"experiments":[119],"indicated":[120],"whole":[127],"kernels":[128],"stable":[130],"50":[132],"1":[138],"week.":[139],"buffer":[141],"composition":[142],"also":[143],"had":[144],"an":[145],"affect":[146],"on":[147],"aqueous":[149],"extraction":[150],"ground":[156],"kernels.":[157],"Avidin":[158],"purified":[160,171,187],"one":[162],"step":[163],"by":[164],"using":[165],"2-iminobiotin":[166],"agarose,":[167],"whereas":[168],"four":[173],"steps":[174],"consisting":[175],"adsorption,":[177],"ion-exchange,":[178],"hydrophobic":[179],"interaction,":[180],"size-exclusion":[182],"chromatography.":[183],"Biochemical":[184],"properties":[185],"similar":[192],"those":[194],"respective":[197],"native":[198],"proteins.":[199]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
