{"id":"https://openalex.org/W2001710064","doi":"https://doi.org/10.1104/pp.68.5.1027","title":"Transfer of Liposome-Sequestering Plasmid DNA into Daucus carota Protoplasts","display_name":"Transfer of Liposome-Sequestering Plasmid DNA into Daucus carota Protoplasts","publication_year":1981,"publication_date":"1981-11-01","ids":{"openalex":"https://openalex.org/W2001710064","doi":"https://doi.org/10.1104/pp.68.5.1027","mag":"2001710064","pmid":"https://pubmed.ncbi.nlm.nih.gov/16662045","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/426039"},"language":"en","primary_location":{"id":"doi:10.1104/pp.68.5.1027","doi":"https://doi.org/10.1104/pp.68.5.1027","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.68.5.1027","pdf_url":"https://academic.oup.com/plphys/article-pdf/68/5/1027/35593152/plphys_v68_5_1027.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/plphys/article-pdf/68/5/1027/35593152/plphys_v68_5_1027.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111838112","display_name":"Hirofumi Uchimiya","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hirofumi Uchimiya","raw_affiliation_strings":["Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki-ken 305, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki-ken 305, Japan","institution_ids":["https://openalex.org/I146399215"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5080837462","display_name":"Hiroshi Harada","orcid":"https://orcid.org/0000-0001-5558-0581","observed_orcids":["https://orcid.org/0000-0001-5558-0581"]},"institutions":[{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroshi Harada","raw_affiliation_strings":["Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki-ken 305, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki-ken 305, Japan","institution_ids":["https://openalex.org/I146399215"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I146399215"],"apc_list":{"value":3851,"currency":"USD","value_usd":3851},"apc_paid":null,"fwci":8.0664,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.97660424,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":91,"max":95},"biblio":{"volume":"68","issue":"5","first_page":"1027","last_page":"1030"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9595000147819519,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9595000147819519,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.032099999487400055,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protoplast-transformation","display_name":"protoplast transformation","score":0.6990000009536743},{"id":"https://openalex.org/keywords/carrot","display_name":"carrot","score":0.9359999895095825},{"id":"https://openalex.org/keywords/pbr322","display_name":"pBR322","score":0.8050000071525574},{"id":"https://openalex.org/keywords/dna-uptake","display_name":"DNA uptake","score":0.628000020980835},{"id":"https://openalex.org/keywords/dna-degradation","display_name":"DNA degradation","score":0.5569999814033508},{"id":"https://openalex.org/keywords/dna-circularization","display_name":"DNA circularization","score":0.31700000166893005}],"concepts":[{"id":"https://openalex.org/C2778842764","wikidata":"https://www.wikidata.org/wiki/Q30056","display_name":"Daucus carota","level":2,"score":0.9834758639335632},{"id":"https://openalex.org/C173749725","wikidata":"https://www.wikidata.org/wiki/Q1126500","display_name":"Protoplast","level":2,"score":0.8119906783103943},{"id":"https://openalex.org/C185154212","wikidata":"https://www.wikidata.org/wiki/Q410454","display_name":"Liposome","level":2,"score":0.5928875803947449},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.5002374649047852},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.48619407415390015},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.46252402663230896},{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.4400690197944641},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42649397253990173},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41630157828330994},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2168060541152954},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18020087480545044}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1104/pp.68.5.1027","doi":"https://doi.org/10.1104/pp.68.5.1027","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.68.5.1027","pdf_url":"https://academic.oup.com/plphys/article-pdf/68/5/1027/35593152/plphys_v68_5_1027.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},{"id":"pmid:16662045","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16662045","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant physiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:426039","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC426039/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"},{"id":"mag:2184871399","doi":null,"is_oa":false,"landing_page_url":"http://www.plantphysiol.org/content/68/5/1027.full.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"mag:2528365687","doi":null,"is_oa":false,"landing_page_url":"https://www.jstor.org/stable/4267042","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.1104/pp.68.5.1027","doi":"https://doi.org/10.1104/pp.68.5.1027","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.68.5.1027","pdf_url":"https://academic.oup.com/plphys/article-pdf/68/5/1027/35593152/plphys_v68_5_1027.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2001710064.pdf","grobid_xml":"https://content.openalex.org/works/W2001710064.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W947965736","https://openalex.org/W962953999","https://openalex.org/W1968181272","https://openalex.org/W1969857197","https://openalex.org/W1988395837","https://openalex.org/W1992261494","https://openalex.org/W2014571604","https://openalex.org/W2016764060","https://openalex.org/W2023689998","https://openalex.org/W2030252157","https://openalex.org/W2044569715","https://openalex.org/W2048522988","https://openalex.org/W2050210628","https://openalex.org/W2053621231","https://openalex.org/W2055155206","https://openalex.org/W2072558942","https://openalex.org/W2072847056","https://openalex.org/W2083665908","https://openalex.org/W2089566170","https://openalex.org/W2395723962","https://openalex.org/W2741720911","https://openalex.org/W4296765338"],"related_works":["https://openalex.org/W2528365687","https://openalex.org/W2184871399","https://openalex.org/W2053304492","https://openalex.org/W2013175868","https://openalex.org/W2020145613","https://openalex.org/W1992261494","https://openalex.org/W2057620935","https://openalex.org/W7095634025","https://openalex.org/W2419462826","https://openalex.org/W944006495"],"abstract_inverted_index":{"Reverse-phase":[0],"evaporation":[1],"lipid":[2],"vesicles":[3],"(REV)":[4],"liposomes,":[5],"consisting":[6],"of":[7,19,44,57,67,85,92,119],"phosphatidyl":[8],"choline":[9],"and":[10,117],"stearylamine":[11],"in":[12,29,61,79,106],"1:3":[13],"molar":[14,52,64],"ratio,":[15],"encapsulated":[16],"approximately":[17],"30%":[18],"exogenously":[20],"supplied":[21],"recombinant":[22],"DNA":[23,27,45,69,87,114,123],"vector,":[24],"pBR322.":[25],"The":[26],"sequestered":[28],"REV":[30],"liposomes":[31,47],"was":[32,39,70,77,101,124],"highly":[33],"tolerant":[34],"to":[35],"DNase.A":[36],"two-step":[37],"procedure":[38],"developed,":[40],"which":[41],"involves":[42],"encapsulation":[43],"with":[46,59,103],"using":[48],"one-tenth":[49],"phosphate-buffered":[50],"saline-0.5":[51],"mannitol,":[53],"followed":[54],"by":[55,97],"incubation":[56],"liposome-DNA":[58],"protoplasts":[60],"phosphate":[62],"buffer-0.5":[63],"mannitol.About":[65],"11%":[66],"liposome-encapsulated":[68],"transferred":[71],"into":[72],"protoplasts,":[73,90],"whereas":[74],"6%":[75],"uptake":[76],"observed":[78],"the":[80,93],"control.":[81],"Although":[82],"some":[83],"degradation":[84],"incorporated":[86],"occurred":[88],"inside":[89],"50%":[91],"total":[94],"radioactivity":[95],"resolved":[96],"0.8%":[98],"agarose":[99],"gel":[100],"associated":[102],"pBR322":[104],"forms":[105],"5-hour":[107],"incubation.":[108],"After":[109],"20-hour":[110],"incubation,":[111],"open":[112],"circular":[113,122],"disappeared":[115],"completely":[116],"maintenance":[118],"covalently":[120],"closed":[121],"confirmed.":[125]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}