{"id":"https://openalex.org/W2001373252","doi":"https://doi.org/10.1006/jmbi.1996.0489","title":"Enzymatic C5-Cytosine Methylation of DNA: Mechanistic Implications of New Crystal Structures forHhaI Methyltransferase-DNA-AdoHcy Complexes","display_name":"Enzymatic C5-Cytosine Methylation of DNA: Mechanistic Implications of New Crystal Structures forHhaI Methyltransferase-DNA-AdoHcy Complexes","publication_year":1996,"publication_date":"1996-09-01","ids":{"openalex":"https://openalex.org/W2001373252","doi":"https://doi.org/10.1006/jmbi.1996.0489","mag":"2001373252","pmid":"https://pubmed.ncbi.nlm.nih.gov/8800212"},"language":"en","primary_location":{"id":"doi:10.1006/jmbi.1996.0489","doi":"https://doi.org/10.1006/jmbi.1996.0489","is_oa":false,"landing_page_url":"https://doi.org/10.1006/jmbi.1996.0489","pdf_url":null,"source":{"id":"https://openalex.org/S46058472","display_name":"Journal of Molecular Biology","issn_l":"0022-2836","issn":["0022-2836","1089-8638"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Molecular Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086582044","display_name":"Margaret O\u2019Gara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]},{"id":"https://openalex.org/I2750212522","display_name":"Cold Spring Harbor Laboratory","ror":"https://ror.org/02qz8b764","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I2750212522"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Margaret O'Gara","raw_affiliation_strings":["W.M. Keck Structural Biology Laboratory Cold Spring Harbor Laboratory, NY 11724, USA","New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA","W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W.M. Keck Structural Biology Laboratory Cold Spring Harbor Laboratory, NY 11724, USA","institution_ids":["https://openalex.org/I2750212522"]},{"raw_affiliation_string":"New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA","institution_ids":["https://openalex.org/I2750212522"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019769228","display_name":"Saulius Klima\u0161auskas","orcid":"https://orcid.org/0000-0002-1395-2030","observed_orcids":["https://orcid.org/0000-0002-1395-2030"]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]},{"id":"https://openalex.org/I2750212522","display_name":"Cold Spring Harbor Laboratory","ror":"https://ror.org/02qz8b764","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I2750212522"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Saulius Klima\u015dauskas","raw_affiliation_strings":["New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA","W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA","institution_ids":["https://openalex.org/I2750212522"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091034547","display_name":"Richard J. Roberts","orcid":"https://orcid.org/0000-0002-4348-0169","observed_orcids":["https://orcid.org/0000-0002-4348-0169"]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]},{"id":"https://openalex.org/I2750212522","display_name":"Cold Spring Harbor Laboratory","ror":"https://ror.org/02qz8b764","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I2750212522"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard J. Roberts","raw_affiliation_strings":["New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA","W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA","institution_ids":["https://openalex.org/I2750212522"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028895511","display_name":"Xiaodong Cheng","orcid":"https://orcid.org/0000-0002-6967-6362","observed_orcids":["https://orcid.org/0000-0002-6967-6362","https://orcid.org/0000-0003-0702-8201"]},"institutions":[{"id":"https://openalex.org/I115020962","display_name":"New England Biolabs (United States)","ror":"https://ror.org/04ywg3445","country_code":"US","type":"company","lineage":["https://openalex.org/I115020962"]},{"id":"https://openalex.org/I2750212522","display_name":"Cold Spring Harbor Laboratory","ror":"https://ror.org/02qz8b764","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I2750212522"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Xiaodong Cheng","raw_affiliation_strings":["New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA","W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA","institution_ids":["https://openalex.org/I115020962"]},{"raw_affiliation_string":"W. M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA","institution_ids":["https://openalex.org/I2750212522"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5028895511"],"corresponding_institution_ids":["https://openalex.org/I115020962","https://openalex.org/I2750212522"],"apc_list":{"value":4730,"currency":"USD","value_usd":4730},"apc_paid":null,"fwci":6.1165,"has_fulltext":false,"cited_by_count":166,"citation_normalized_percentile":{"value":0.97021812,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"261","issue":"5","first_page":"634","last_page":"645"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.5824999809265137,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.5824999809265137,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.2037000060081482,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.031599998474121094,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-methylation","display_name":"DNA methylation","score":0.828000009059906},{"id":"https://openalex.org/keywords/s-adenosylhomocysteine","display_name":"S-adenosylhomocysteine","score":0.968999981880188},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.597000002861023},{"id":"https://openalex.org/keywords/reaction-mechanism","display_name":"reaction mechanism","score":0.6549999713897705},{"id":"https://openalex.org/keywords/active-site","display_name":"active site","score":0.6589999794960022},{"id":"https://openalex.org/keywords/s-adenosylmethionine","display_name":"S-adenosylmethionine","score":0.796999990940094},{"id":"https://openalex.org/keywords/2-deoxycytidine","display_name":"2'-deoxycytidine","score":0.6949999928474426}],"concepts":[{"id":"https://openalex.org/C2776023528","wikidata":"https://www.wikidata.org/wiki/Q178425","display_name":"Cytosine","level":3,"score":0.7652701139450073},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7067307233810425},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6045895218849182},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5702410936355591},{"id":"https://openalex.org/C33288867","wikidata":"https://www.wikidata.org/wiki/Q518328","display_name":"Methylation","level":3,"score":0.5209559202194214},{"id":"https://openalex.org/C91965660","wikidata":"https://www.wikidata.org/wiki/Q415875","display_name":"Methyltransferase","level":4,"score":0.5075885057449341},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.4549337327480316},{"id":"https://openalex.org/C2778565081","wikidata":"https://www.wikidata.org/wiki/Q4407","display_name":"Methyl group","level":3,"score":0.4509453773498535},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.42589038610458374},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28138458728790283},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2502734363079071},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C2780263894","wikidata":"https://www.wikidata.org/wiki/Q335268","display_name":"Alkyl","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003596","descriptor_name":"Cytosine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003596","descriptor_name":"Cytosine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008715","descriptor_name":"Methionine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D008715","descriptor_name":"Methionine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008715","descriptor_name":"Methionine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011522","descriptor_name":"Protons","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012435","descriptor_name":"S-Adenosylhomocysteine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015257","descriptor_name":"DNA-Cytosine Methylases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015257","descriptor_name":"DNA-Cytosine Methylases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019175","descriptor_name":"DNA Methylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1006/jmbi.1996.0489","doi":"https://doi.org/10.1006/jmbi.1996.0489","is_oa":false,"landing_page_url":"https://doi.org/10.1006/jmbi.1996.0489","pdf_url":null,"source":{"id":"https://openalex.org/S46058472","display_name":"Journal of Molecular Biology","issn_l":"0022-2836","issn":["0022-2836","1089-8638"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Molecular Biology","raw_type":"journal-article"},{"id":"pmid:8800212","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8800212","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of molecular biology","raw_type":"Comparative Study"},{"id":"pmh:oai:repository.cshl.edu:30096","doi":null,"is_oa":false,"landing_page_url":"https://repository.cshl.edu/id/eprint/30096/","pdf_url":null,"source":{"id":"https://openalex.org/S4306402288","display_name":"Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Paper"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1906145986","display_name":"STRUCTURAL STUDIES OF METHYLTRANSFERASE/DNA COMPLEXES","funder_award_id":"F32GM017052","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6187331283","display_name":null,"funder_award_id":"ZT32CA09311-16","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G8931618847","display_name":"M5 CYTOSINE METHYLATION","funder_award_id":"R01GM046127","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306084","display_name":"U.S. Department of Energy","ror":"https://ror.org/01bj3aw27"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320338289","display_name":"Brookhaven National Laboratory","ror":"https://ror.org/02ex6cf31"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2077890483","https://openalex.org/W2313496082","https://openalex.org/W2006419303","https://openalex.org/W1585338920","https://openalex.org/W2000168874","https://openalex.org/W2085386155","https://openalex.org/W1994162276","https://openalex.org/W1986446731","https://openalex.org/W1997946940","https://openalex.org/W1991663087"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}