{"id":"https://openalex.org/W2001237138","doi":"https://doi.org/10.1016/j.ab.2008.11.018","title":"Development and application of a differential method for reliable metabolome analysis in Escherichia coli","display_name":"Development and application of a differential method for reliable metabolome analysis in Escherichia coli","publication_year":2008,"publication_date":"2008-11-26","ids":{"openalex":"https://openalex.org/W2001237138","doi":"https://doi.org/10.1016/j.ab.2008.11.018","mag":"2001237138","pmid":"https://pubmed.ncbi.nlm.nih.gov/19084496"},"language":"en","primary_location":{"id":"doi:10.1016/j.ab.2008.11.018","doi":"https://doi.org/10.1016/j.ab.2008.11.018","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.ab.2008.11.018","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://biblio.ugent.be/publication/673446/file/876499.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091757000","display_name":"Hilal Taymaz\u2010Nikerel","orcid":"https://orcid.org/0000-0002-5523-263X","observed_orcids":["https://orcid.org/0000-0002-5523-263X"]},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Hilal Taymaz-Nikerel","raw_affiliation_strings":["Department of Biotechnology, Delft University of Technology, BC Delft, The Netherlands","Department of Biotechnology, Delft University of Technology, BC, Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, BC Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]},{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, BC, Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026523298","display_name":"Marjan De Mey","orcid":"https://orcid.org/0000-0002-8134-9436","observed_orcids":["https://orcid.org/0000-0002-8134-9436"]},"institutions":[{"id":"https://openalex.org/I32597200","display_name":"Ghent University","ror":"https://ror.org/00cv9y106","country_code":"BE","type":"education","lineage":["https://openalex.org/I32597200"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Marjan de Mey","raw_affiliation_strings":["Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, B-9000 Ghent, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, B-9000 Ghent, Belgium","institution_ids":["https://openalex.org/I32597200"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055939409","display_name":"Cor Ras","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Cor Ras","raw_affiliation_strings":["Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017592884","display_name":"Angela ten Pierick","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Angela ten Pierick","raw_affiliation_strings":["Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035527719","display_name":"Reza M. Seifar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Reza M. Seifar","raw_affiliation_strings":["Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047069259","display_name":"Jan C. van Dam","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Jan C. van Dam","raw_affiliation_strings":["Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090214575","display_name":"Joseph J. Heijnen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Joseph J. Heijnen","raw_affiliation_strings":["Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015782458","display_name":"Walter M. van Gulik","orcid":"https://orcid.org/0000-0002-2626-4288","observed_orcids":["https://orcid.org/0000-0002-2626-4288"]},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"Walter M. van Gulik","raw_affiliation_strings":["Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5015782458"],"corresponding_institution_ids":["https://openalex.org/I98358874"],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":4.6012,"has_fulltext":true,"cited_by_count":148,"citation_normalized_percentile":{"value":0.95726763,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":100},"biblio":{"volume":"386","issue":"1","first_page":"9","last_page":"19"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.8130000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.8130000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.17579999566078186,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/extracellular-metabolites","display_name":"extracellular metabolites","score":0.949999988079071},{"id":"https://openalex.org/keywords/intracellular-metabolites","display_name":"intracellular metabolites","score":0.7829999923706055},{"id":"https://openalex.org/keywords/chemostat-culture","display_name":"chemostat culture","score":0.5950000286102295},{"id":"https://openalex.org/keywords/amino-acids","display_name":"amino acids","score":0.609000027179718}],"concepts":[{"id":"https://openalex.org/C135870905","wikidata":"https://www.wikidata.org/wiki/Q903667","display_name":"Metabolome","level":3,"score":0.9226110577583313},{"id":"https://openalex.org/C125111812","wikidata":"https://www.wikidata.org/wiki/Q902311","display_name":"Chemostat","level":3,"score":0.7884745597839355},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.7096652388572693},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.6613378524780273},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6189790368080139},{"id":"https://openalex.org/C121745418","wikidata":"https://www.wikidata.org/wiki/Q585536","display_name":"Quenching (fluorescence)","level":3,"score":0.6184735298156738},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5865750908851624},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4125255346298218},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3913125991821289},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38649410009384155},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.2812795042991638},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.11567145586013794},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005374","descriptor_name":"Filtration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005374","descriptor_name":"Filtration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005374","descriptor_name":"Filtration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008722","descriptor_name":"Methods","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008722","descriptor_name":"Methods","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008722","descriptor_name":"Methods","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012107","descriptor_name":"Research Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012107","descriptor_name":"Research Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012107","descriptor_name":"Research Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055442","descriptor_name":"Metabolome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D055442","descriptor_name":"Metabolome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D055442","descriptor_name":"Metabolome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.ab.2008.11.018","doi":"https://doi.org/10.1016/j.ab.2008.11.018","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.ab.2008.11.018","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:19084496","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19084496","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Evaluation Study"},{"id":"pmh:oai:archive.ugent.be:673446","doi":null,"is_oa":true,"landing_page_url":"https://biblio.ugent.be/publication/673446","pdf_url":"https://biblio.ugent.be/publication/673446/file/876499.pdf","source":{"id":"https://openalex.org/S4306400478","display_name":"Ghent University Academic Bibliography (Ghent University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32597200","host_organization_name":"Ghent University","host_organization_lineage":["https://openalex.org/I32597200"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ISSN: 0003-2697","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"pmh:oai:archive.ugent.be:673446","doi":null,"is_oa":true,"landing_page_url":"https://biblio.ugent.be/publication/673446","pdf_url":"https://biblio.ugent.be/publication/673446/file/876499.pdf","source":{"id":"https://openalex.org/S4306400478","display_name":"Ghent University Academic Bibliography (Ghent University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32597200","host_organization_name":"Ghent University","host_organization_lineage":["https://openalex.org/I32597200"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ISSN: 0003-2697","raw_type":"info:eu-repo/semantics/article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Clean water and sanitation","id":"https://metadata.un.org/sdg/6","score":0.5199999809265137}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320327336","display_name":"Vlaamse regering","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2001237138.pdf","grobid_xml":"https://content.openalex.org/works/W2001237138.grobid-xml"},"referenced_works_count":57,"referenced_works":["https://openalex.org/W17356407","https://openalex.org/W653553412","https://openalex.org/W1499814836","https://openalex.org/W1582646265","https://openalex.org/W1797657318","https://openalex.org/W1966645006","https://openalex.org/W1967437164","https://openalex.org/W1974927365","https://openalex.org/W1975204049","https://openalex.org/W1975211610","https://openalex.org/W1976353541","https://openalex.org/W1988537291","https://openalex.org/W1990520393","https://openalex.org/W1994683277","https://openalex.org/W1997493743","https://openalex.org/W1997696539","https://openalex.org/W1998034857","https://openalex.org/W2003240435","https://openalex.org/W2007378834","https://openalex.org/W2009200103","https://openalex.org/W2011668869","https://openalex.org/W2016140326","https://openalex.org/W2023216808","https://openalex.org/W2026481834","https://openalex.org/W2028400128","https://openalex.org/W2033570693","https://openalex.org/W2037928747","https://openalex.org/W2050858598","https://openalex.org/W2053339983","https://openalex.org/W2054744694","https://openalex.org/W2073253360","https://openalex.org/W2079652596","https://openalex.org/W2086934275","https://openalex.org/W2092504964","https://openalex.org/W2101442503","https://openalex.org/W2102113984","https://openalex.org/W2112258048","https://openalex.org/W2119711811","https://openalex.org/W2119726180","https://openalex.org/W2123725317","https://openalex.org/W2124804155","https://openalex.org/W2126095268","https://openalex.org/W2128256405","https://openalex.org/W2133505559","https://openalex.org/W2135809413","https://openalex.org/W2136568972","https://openalex.org/W2143893946","https://openalex.org/W2152873168","https://openalex.org/W2155151971","https://openalex.org/W2155250997","https://openalex.org/W2159687660","https://openalex.org/W2163388517","https://openalex.org/W2166866734","https://openalex.org/W2169559175","https://openalex.org/W2170576942","https://openalex.org/W2221566904","https://openalex.org/W4235920957"],"related_works":["https://openalex.org/W1996644818","https://openalex.org/W1965468642","https://openalex.org/W2093500216","https://openalex.org/W2053588008","https://openalex.org/W2916376502","https://openalex.org/W2260452057","https://openalex.org/W3101161779","https://openalex.org/W423371831","https://openalex.org/W2777729175","https://openalex.org/W4295779760"],"abstract_inverted_index":null,"counts_by_year":[{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":12},{"year":2016,"cited_by_count":13},{"year":2015,"cited_by_count":17},{"year":2014,"cited_by_count":15},{"year":2013,"cited_by_count":15},{"year":2012,"cited_by_count":12}],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2025-10-10T00:00:00"}
