{"id":"https://openalex.org/W2000979138","doi":"https://doi.org/10.1002/1522-2683(200101)22:1<18::aid-elps18>3.0.co;2-7","title":"Contribution of blotting techniques to the study of rapeseeds (Brassica napus L.) lipases","display_name":"Contribution of blotting techniques to the study of rapeseeds (Brassica napus L.) lipases","publication_year":2001,"publication_date":"2001-01-01","ids":{"openalex":"https://openalex.org/W2000979138","doi":"https://doi.org/10.1002/1522-2683(200101)22:1<18::aid-elps18>3.0.co;2-7","mag":"2000979138","pmid":"https://pubmed.ncbi.nlm.nih.gov/11197170"},"language":"en","primary_location":{"id":"doi:10.1002/1522-2683(200101)22:1<18::aid-elps18>3.0.co;2-7","doi":"https://doi.org/10.1002/1522-2683(200101)22:1<18::aid-elps18>3.0.co;2-7","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1522-2683(200101)22:1<18::aid-elps18>3.0.co;2-7","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028085404","display_name":"Hatem Belguith","orcid":"https://orcid.org/0000-0002-5601-3834","observed_orcids":["https://orcid.org/0000-0002-5601-3834"]},"institutions":[{"id":"https://openalex.org/I108714496","display_name":"Tunis University","ror":"https://ror.org/02q1spa57","country_code":"TN","type":"education","lineage":["https://openalex.org/I108714496"]}],"countries":["TN"],"is_corresponding":false,"raw_author_name":"Hatem Belguith","raw_affiliation_strings":["Laboratoire de Biochimie des prot\u00e9ines, Facult\u00e9 des Sciences de Tunis, Tunis, Tunisia","Laboratoire de Biochimie des prot\u00e9ines"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie des prot\u00e9ines, Facult\u00e9 des Sciences de Tunis, Tunis, Tunisia","institution_ids":["https://openalex.org/I108714496"]},{"raw_affiliation_string":"Laboratoire de Biochimie des prot\u00e9ines","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036866393","display_name":"Houssein Khodjet\u2010El\u2010Khil","orcid":"https://orcid.org/0000-0003-1020-8094","observed_orcids":["https://orcid.org/0000-0003-1020-8094"]},"institutions":[{"id":"https://openalex.org/I108714496","display_name":"Tunis University","ror":"https://ror.org/02q1spa57","country_code":"TN","type":"education","lineage":["https://openalex.org/I108714496"]}],"countries":["TN"],"is_corresponding":false,"raw_author_name":"Houssein Khodjet El Khil","raw_affiliation_strings":["Laboratoire d'Immuno-Ge\u0301ne\u0301tique, Faculte\u0301 des Sciences de Tunis, Tunis, Tunisia","Laboratoire d'Immuno\u2010G\u00e9n\u00e9tique, Facult\u00e9 des Sciences de Tunis, Tunis, Tunisia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire d'Immuno-Ge\u0301ne\u0301tique, Faculte\u0301 des Sciences de Tunis, Tunis, Tunisia","institution_ids":["https://openalex.org/I108714496"]},{"raw_affiliation_string":"Laboratoire d'Immuno\u2010G\u00e9n\u00e9tique, Facult\u00e9 des Sciences de Tunis, Tunis, Tunisia","institution_ids":["https://openalex.org/I108714496"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053426522","display_name":"Sami Fattouch","orcid":"https://orcid.org/0000-0001-7317-8895","observed_orcids":["https://orcid.org/0000-0001-7317-8895"]},"institutions":[{"id":"https://openalex.org/I108714496","display_name":"Tunis University","ror":"https://ror.org/02q1spa57","country_code":"TN","type":"education","lineage":["https://openalex.org/I108714496"]}],"countries":["TN"],"is_corresponding":false,"raw_author_name":"Sami Fattouch","raw_affiliation_strings":["Laboratoire d'Immuno-Ge\u0301ne\u0301tique, Faculte\u0301 des Sciences de Tunis, Tunis, Tunisia","Laboratoire d'Immuno\u2010G\u00e9n\u00e9tique, Facult\u00e9 des Sciences de Tunis, Tunis, Tunisia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire d'Immuno-Ge\u0301ne\u0301tique, Faculte\u0301 des Sciences de Tunis, Tunis, Tunisia","institution_ids":["https://openalex.org/I108714496"]},{"raw_affiliation_string":"Laboratoire d'Immuno\u2010G\u00e9n\u00e9tique, Facult\u00e9 des Sciences de Tunis, Tunis, Tunisia","institution_ids":["https://openalex.org/I108714496"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076951034","display_name":"T Jridi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Toufik Jridi","raw_affiliation_strings":["Laboratoire de Biochimie des prote\u0301ines","Laboratoire de Biochimie des prot\u00e9ines"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie des prote\u0301ines","institution_ids":[]},{"raw_affiliation_string":"Laboratoire de Biochimie des prot\u00e9ines","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108527106","display_name":"Jeannette Ben Hamida","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I108714496","display_name":"Tunis University","ror":"https://ror.org/02q1spa57","country_code":"TN","type":"education","lineage":["https://openalex.org/I108714496"]}],"countries":["TN"],"is_corresponding":true,"raw_author_name":"Jeannette Ben Hamida","raw_affiliation_strings":["Laboratoire de Biochimie des prote\u0301ines","Facult\u00e9 des Sciences de Tunis, D\u00e9partement de Biologie, Campus universitaire, 1060 Tunis, Tunisia, Fax: +216(1)\u2010871 666"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie des prote\u0301ines","institution_ids":[]},{"raw_affiliation_string":"Facult\u00e9 des Sciences de Tunis, D\u00e9partement de Biologie, Campus universitaire, 1060 Tunis, Tunisia, Fax: +216(1)\u2010871 666","institution_ids":["https://openalex.org/I108714496"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5108527106"],"corresponding_institution_ids":["https://openalex.org/I108714496"],"apc_list":{"value":3940,"currency":"USD","value_usd":3940},"apc_paid":null,"fwci":0.1201,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.31891755,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":95},"biblio":{"volume":"22","issue":"1","first_page":"18","last_page":"22"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brassica-napus","display_name":"Brassica napus","score":0.8510000109672546},{"id":"https://openalex.org/keywords/plant-lipases","display_name":"plant lipases","score":0.8610000014305115},{"id":"https://openalex.org/keywords/porcine-pancreatic-lipase","display_name":"porcine pancreatic lipase","score":0.8519999980926514},{"id":"https://openalex.org/keywords/immunological-cross-reactivity","display_name":"immunological cross-reactivity","score":0.7730000019073486},{"id":"https://openalex.org/keywords/cotyledons","display_name":"cotyledons","score":0.746999979019165},{"id":"https://openalex.org/keywords/seedling-germination","display_name":"seedling germination","score":0.7049999833106995},{"id":"https://openalex.org/keywords/western-blot","display_name":"Western blot","score":0.6890000104904175},{"id":"https://openalex.org/keywords/enzyme-linked-immunosorbent-assay","display_name":"enzyme-linked immunosorbent assay","score":0.8199999928474426},{"id":"https://openalex.org/keywords/dot-blot-hybridization","display_name":"dot blot","score":0.7739999890327454},{"id":"https://openalex.org/keywords/immunoprecipitation","display_name":"immunoprecipitation","score":0.8970000147819519},{"id":"https://openalex.org/keywords/catalytic-residues","display_name":"catalytic residues","score":0.625},{"id":"https://openalex.org/keywords/epitopes","display_name":"antigenic determinants","score":0.42800000309944153}],"concepts":[{"id":"https://openalex.org/C2779726014","wikidata":"https://www.wikidata.org/wiki/Q177932","display_name":"Rapeseed","level":2,"score":0.8726217746734619},{"id":"https://openalex.org/C2779697368","wikidata":"https://www.wikidata.org/wiki/Q380967","display_name":"Lipase","level":3,"score":0.8549503087997437},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.8032859563827515},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6612964272499084},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.6588731408119202},{"id":"https://openalex.org/C71829478","wikidata":"https://www.wikidata.org/wiki/Q901305","display_name":"Immunoprecipitation","level":3,"score":0.640659749507904},{"id":"https://openalex.org/C175106660","wikidata":"https://www.wikidata.org/wiki/Q424693","display_name":"Pancreatic lipase","level":4,"score":0.6211556196212769},{"id":"https://openalex.org/C132946820","wikidata":"https://www.wikidata.org/wiki/Q2509459","display_name":"Differential centrifugation","level":2,"score":0.600939154624939},{"id":"https://openalex.org/C2779142602","wikidata":"https://www.wikidata.org/wiki/Q1760689","display_name":"Cross-reactivity","level":4,"score":0.5465289950370789},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5433983206748962},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5119270086288452},{"id":"https://openalex.org/C2780786110","wikidata":"https://www.wikidata.org/wiki/Q7839871","display_name":"Triacylglycerol lipase","level":4,"score":0.508602499961853},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.47151079773902893},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.444664865732193},{"id":"https://openalex.org/C127947577","wikidata":"https://www.wikidata.org/wiki/Q3682656","display_name":"Colipase","level":5,"score":0.4220309555530548},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4215855598449707},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42149531841278076},{"id":"https://openalex.org/C2776747608","wikidata":"https://www.wikidata.org/wiki/Q58677","display_name":"Brassica","level":2,"score":0.41400572657585144},{"id":"https://openalex.org/C3020064264","wikidata":"https://www.wikidata.org/wiki/Q1760689","display_name":"Cross reactions","level":3,"score":0.14968544244766235},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.12726423144340515},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.08871433138847351},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.06221005320549011},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001937","descriptor_name":"Brassica","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008049","descriptor_name":"Lipase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012639","descriptor_name":"Seeds","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014280","descriptor_name":"Triglycerides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D018548","descriptor_name":"Cotyledon","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/1522-2683(200101)22:1<18::aid-elps18>3.0.co;2-7","doi":"https://doi.org/10.1002/1522-2683(200101)22:1<18::aid-elps18>3.0.co;2-7","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1522-2683(200101)22:1<18::aid-elps18>3.0.co;2-7","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},{"id":"pmid:11197170","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11197170","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1508885706","https://openalex.org/W1797616957","https://openalex.org/W1845386550","https://openalex.org/W1977832025","https://openalex.org/W1992249402","https://openalex.org/W2006632715","https://openalex.org/W2008252401","https://openalex.org/W2014258129","https://openalex.org/W2052607964","https://openalex.org/W2058623892","https://openalex.org/W2072812418","https://openalex.org/W2086829136","https://openalex.org/W2100837269","https://openalex.org/W2127963615","https://openalex.org/W2128635872","https://openalex.org/W2409415618","https://openalex.org/W4210252797"],"related_works":["https://openalex.org/W4248343177","https://openalex.org/W285836431","https://openalex.org/W2084073157","https://openalex.org/W2104688733","https://openalex.org/W2125572287","https://openalex.org/W2284913090","https://openalex.org/W2058623892","https://openalex.org/W2511025645","https://openalex.org/W2096185619","https://openalex.org/W2482181004"],"abstract_inverted_index":{"A":[0],"recent":[1],"advance":[2],"in":[3,43,60,66,150,162],"the":[4,61,67,77,80,85,91,106,147,154],"study":[5,152],"of":[6,20,76,87,124],"plant":[7],"lipases":[8,19,59],"involving":[9],"immunological":[10],"techniques":[11],"is":[12,46,110],"presented.":[13],"In":[14],"an":[15,29],"attempt":[16],"to":[17,27,90,116,139],"characterize":[18],"cotyledons":[21],"from":[22,146],"germinating":[23],"rapeseed":[24,62,81,100],"seedlings":[25],"and":[26,52,65,101,166],"investigate":[28],"eventual":[30],"cross-reactivity":[31,98],"with":[32,58,79,120],"animal":[33],"lipases,":[34],"we":[35],"have":[36],"prepared":[37],"anti-porcine":[38],"pancreatic":[39,93,103],"lipase":[40,129,157],"antibodies":[41,56,88,113,136],"raised":[42],"rabbit.":[44],"It":[45],"shown":[47],"by":[48,72],"enzyme-linked":[49],"immunosorbent":[50],"assay":[51],"dot-blotting":[53],"that":[54,112,135,153],"these":[55],"react":[57],"crude":[63,82],"extract":[64,83],"different":[68,156],"cellular":[69],"fractions":[70],"obtained":[71,149],"differential":[73],"centrifugation.":[74],"Preincubation":[75],"antiserum":[78],"affects":[84],"amount":[86],"binding":[89],"porcine":[92,102],"lipase.":[94,104],"We":[95,144],"demonstrate":[96],"immunochemical":[97],"between":[99],"Using":[105],"immunoblotting":[107],"procedure,":[108],"it":[109],"found":[111],"bind":[114],"specifically":[115],"a":[117,121],"single":[118],"polypeptide":[119],"molecular":[122],"mass":[123],"about":[125],"55":[126],"kDa.":[127],"Rapeseed":[128],"activity":[130],"decreased":[131],"after":[132],"immunoprecipitation":[133],"suggesting":[134],"were":[137],"bound":[138],"some":[140],"catalytic":[141],"site":[142],"residues.":[143],"conclude":[145],"data":[148],"this":[151],"two":[155],"species":[158],"present":[159],"close":[160],"similarities":[161],"amino":[163],"acid":[164],"sequence":[165],"antigen":[167],"characteristics.":[168]},"counts_by_year":[{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}