{"id":"https://openalex.org/W2000888410","doi":"https://doi.org/10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g","title":"Recombinant protein production driven by the tryptophan promoter is tightly controlled in ICONE 200, a new genetically engineeredE. coli mutant","display_name":"Recombinant protein production driven by the tryptophan promoter is tightly controlled in ICONE 200, a new genetically engineeredE. coli mutant","publication_year":2000,"publication_date":"2000-01-01","ids":{"openalex":"https://openalex.org/W2000888410","doi":"https://doi.org/10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g","mag":"2000888410","pmid":"https://pubmed.ncbi.nlm.nih.gov/10862673"},"language":"en","primary_location":{"id":"doi:10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g","doi":"https://doi.org/10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012137402","display_name":"Laurent Chevalet","orcid":"https://orcid.org/0009-0003-5802-7353","observed_orcids":["https://orcid.org/0009-0003-5802-7353"]},"institutions":[{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"L. Chevalet","raw_affiliation_strings":["Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France. laurent.chevalet@pierre-fabre.com","Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","fax: +33 4 50 35 35 90","telephone: +33 4 50 35 35 18"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France. laurent.chevalet@pierre-fabre.com","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"fax: +33 4 50 35 35 90","institution_ids":[]},{"raw_affiliation_string":"telephone: +33 4 50 35 35 18","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"A. Robert","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"A. Robert","raw_affiliation_strings":["Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France; telephone: +33 4 50 35 35 18; fax: +33 4 50 35 35 90","Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","fax: +33 4 50 35 35 90","telephone: +33 4 50 35 35 18"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France; telephone: +33 4 50 35 35 18; fax: +33 4 50 35 35 90","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"fax: +33 4 50 35 35 90","institution_ids":[]},{"raw_affiliation_string":"telephone: +33 4 50 35 35 18","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053181303","display_name":"Franck Gueneau","orcid":"https://orcid.org/0000-0003-0054-6575","observed_orcids":["https://orcid.org/0000-0003-0054-6575"]},"institutions":[{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"F. Gueneau","raw_affiliation_strings":["Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France; telephone: +33 4 50 35 35 18; fax: +33 4 50 35 35 90","Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","fax: +33 4 50 35 35 90","telephone: +33 4 50 35 35 18"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France; telephone: +33 4 50 35 35 18; fax: +33 4 50 35 35 90","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"fax: +33 4 50 35 35 90","institution_ids":[]},{"raw_affiliation_string":"telephone: +33 4 50 35 35 18","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109908396","display_name":"Jean\u2010Yves Bonnefoy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"J.-Y. Bonnefoy","raw_affiliation_strings":["Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France; telephone: +33 4 50 35 35 18; fax: +33 4 50 35 35 90","Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","fax: +33 4 50 35 35 90","telephone: +33 4 50 35 35 18"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France; telephone: +33 4 50 35 35 18; fax: +33 4 50 35 35 90","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"fax: +33 4 50 35 35 90","institution_ids":[]},{"raw_affiliation_string":"telephone: +33 4 50 35 35 18","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5052561639","display_name":"Thien Ngoc Nguyen","orcid":"https://orcid.org/0000-0003-4575-0422","observed_orcids":["https://orcid.org/0000-0003-4575-0422"]},"institutions":[{"id":"https://openalex.org/I4210135355","display_name":"Center of Immunology Pierre Fabre","ror":"https://ror.org/03gd99e09","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210135355","https://openalex.org/I4210143394"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"T. Nguyen","raw_affiliation_strings":["Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France; telephone: +33 4 50 35 35 18; fax: +33 4 50 35 35 90","Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","fax: +33 4 50 35 35 90","telephone: +33 4 50 35 35 18"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France; telephone: +33 4 50 35 35 18; fax: +33 4 50 35 35 90","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"Centre d'Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint-Julien en Genevois, France","institution_ids":["https://openalex.org/I4210135355"]},{"raw_affiliation_string":"fax: +33 4 50 35 35 90","institution_ids":[]},{"raw_affiliation_string":"telephone: +33 4 50 35 35 18","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5012137402"],"corresponding_institution_ids":["https://openalex.org/I4210135355"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":0.319,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.55372549,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"69","issue":"4","first_page":"351","last_page":"358"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.7235999703407288,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.7235999703407288,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.2590000033378601,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.005799999926239252,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tryptophan-operon","display_name":"tryptophan operon","score":0.828000009059906},{"id":"https://openalex.org/keywords/trpr","display_name":"TrpR","score":0.671999990940094},{"id":"https://openalex.org/keywords/tnaa","display_name":"tnaA","score":0.843999981880188},{"id":"https://openalex.org/keywords/tryptophanase","display_name":"tryptophanase","score":0.9079999923706055},{"id":"https://openalex.org/keywords/inducible-gene-expression","display_name":"inducible gene expression","score":0.5699999928474426},{"id":"https://openalex.org/keywords/hiv-1-protease","display_name":"HIV-1 protease","score":0.996999979019165},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8289999961853027}],"concepts":[{"id":"https://openalex.org/C158448853","wikidata":"https://www.wikidata.org/wiki/Q425218","display_name":"Repressor","level":4,"score":0.7458645105361938},{"id":"https://openalex.org/C203075996","wikidata":"https://www.wikidata.org/wiki/Q139677","display_name":"Operon","level":4,"score":0.7335325479507446},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6405945420265198},{"id":"https://openalex.org/C2776706248","wikidata":"https://www.wikidata.org/wiki/Q181003","display_name":"Tryptophan","level":3,"score":0.6109488606452942},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6022294759750366},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5366268754005432},{"id":"https://openalex.org/C96601446","wikidata":"https://www.wikidata.org/wiki/Q287043","display_name":"trp operon","level":5,"score":0.5142797827720642},{"id":"https://openalex.org/C114847915","wikidata":"https://www.wikidata.org/wiki/Q16948788","display_name":"Ribosomal binding site","level":5,"score":0.5027155876159668},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.49953126907348633},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.453274130821228},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.4507938027381897},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.4456791579723358},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.4386696517467499},{"id":"https://openalex.org/C2778526059","wikidata":"https://www.wikidata.org/wiki/Q3693596","display_name":"Chorismate mutase","level":4,"score":0.41004183888435364},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3679054379463196},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.33759647607803345},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.15827468037605286},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.15377578139305115},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.09394785761833191},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.07298001646995544}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D012097","descriptor_name":"Repressor Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012097","descriptor_name":"Repressor Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015964","descriptor_name":"Gene Expression Regulation, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016333","descriptor_name":"HIV Protease","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D016333","descriptor_name":"HIV Protease","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017361","descriptor_name":"Viral Nonstructural Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D017361","descriptor_name":"Viral Nonstructural Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g","doi":"https://doi.org/10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1097-0290(20000820)69:4<351::aid-bit1>3.0.co;2-g","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:10862673","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10862673","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W25080383","https://openalex.org/W328595349","https://openalex.org/W1423590398","https://openalex.org/W1490719520","https://openalex.org/W1493909852","https://openalex.org/W1499790908","https://openalex.org/W1531494225","https://openalex.org/W1572116252","https://openalex.org/W1576795553","https://openalex.org/W1583486230","https://openalex.org/W1753916721","https://openalex.org/W1895819679","https://openalex.org/W1910590028","https://openalex.org/W1913059693","https://openalex.org/W1970634829","https://openalex.org/W1987032620","https://openalex.org/W1994088766","https://openalex.org/W2003250816","https://openalex.org/W2017429344","https://openalex.org/W2018146412","https://openalex.org/W2038894392","https://openalex.org/W2039107470","https://openalex.org/W2059733029","https://openalex.org/W2068624029","https://openalex.org/W2070308600","https://openalex.org/W2078283172","https://openalex.org/W2083613414","https://openalex.org/W2089019538","https://openalex.org/W2107205153","https://openalex.org/W2137490166","https://openalex.org/W2157217176","https://openalex.org/W2160084442","https://openalex.org/W2206701392","https://openalex.org/W2314225431","https://openalex.org/W3184653731"],"related_works":["https://openalex.org/W2147309731","https://openalex.org/W2595118077","https://openalex.org/W2019790468","https://openalex.org/W2359733817","https://openalex.org/W2806065713","https://openalex.org/W1983955790","https://openalex.org/W496513183","https://openalex.org/W3184653731","https://openalex.org/W2385643264","https://openalex.org/W2393464438"],"abstract_inverted_index":{"Batch":[0],"processes":[1],"for":[2,87,204],"recombinant":[3,38],"gene":[4,103],"expression":[5,36,48,138],"in":[6,40,62,117,142,152],"prokaryotic":[7],"systems":[8],"should":[9],"optimally":[10],"comprise":[11],"a":[12,21,53,72,172,191,202],"growth":[13],"phase":[14,23],"with":[15,100],"minimal":[16],"promoter":[17,28],"activity":[18],"followed":[19],"by":[20],"short":[22],"favoring":[24],"expression.":[25],"The":[26,43],"strong":[27],"of":[29,37,59,65,94,107,111,123,145,175],"the":[30,57,63,96,101,105,132,143,163,168],"tryptophan":[31,146,159],"operon":[32],"(Ptrp)":[33],"gives":[34],"high-level":[35],"proteins":[39,176,179],"E.":[41,74,154],"coli.":[42],"inefficiency":[44],"to":[45,119,128,170],"control":[46],"basal":[47],"before":[49],"induction":[50],"is":[51,190],"however":[52],"major":[54],"obstacle":[55],"towards":[56],"use":[58],"Ptrp,":[60],"especially":[61],"case":[64],"toxic":[66,178],"proteins.":[67],"To":[68],"circumvent":[69],"this":[70],"problem,":[71],"novel":[73],"coli":[75],"strain":[76],"has":[77,167],"been":[78],"generated.":[79],"This":[80],"mutant,":[81],"named":[82],"ICONE":[83,112,165,188],"200":[84,113,166,189],"(Improved":[85],"Cell":[86],"Over":[88],"and":[89,147,184,197,201],"Non-leaky":[90],"Expression),":[91],"underwent":[92],"replacement":[93],"tnaA,":[95],"tryptophanase":[97,134],"encoding":[98,104],"gene,":[99],"trpR":[102,130],"aporepressor":[106],"Ptrp.":[108],"Detailed":[109],"analysis":[110],"showed":[114],"that":[115],"tryptophan,":[116],"addition":[118],"its":[120],"natural":[121],"role":[122],"Ptrp":[124],"co-repressor,":[125],"was":[126,139,148,160],"able":[127],"induce":[129],"through":[131],"tryptophan-inducible":[133],"promoter/operator.":[135],"Consequently,":[136],"Ptrp-dependent":[137],"efficiently":[140],"repressed":[141],"presence":[144],"turned":[149],"on,":[150],"as":[151,156,158,181],"wild-type":[153],"coli,":[155],"soon":[157],"exhausted":[161],"from":[162],"medium.":[164],"capacity":[169],"express":[171],"wide":[173],"range":[174],"including":[177],"such":[180],"HIV-1":[182],"protease":[183],"poliovirus":[185],"2B":[186],"protein.":[187],"new":[192],"host":[193],"carrying":[194],"stable,":[195],"targeted,":[196],"marker-free":[198],"genetic":[199],"modifications":[200],"candidate":[203],"large-scale":[205],"applications.":[206]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
