{"id":"https://openalex.org/W200085130","doi":null,"title":"A new and improved \"quick-hot Gram-chromotrope\" technique that differentially stains microsporidian spores in clinical samples, including paraffin-embedded tissue sections.","display_name":"A new and improved \"quick-hot Gram-chromotrope\" technique that differentially stains microsporidian spores in clinical samples, including paraffin-embedded tissue sections.","publication_year":1997,"publication_date":"1997-08-01","ids":{"openalex":"https://openalex.org/W200085130","mag":"200085130","pmid":"https://pubmed.ncbi.nlm.nih.gov/9278620"},"language":"en","primary_location":{"id":"pmid:9278620","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9278620","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of pathology & laboratory medicine","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076114542","display_name":"H\u00e9rcules Moura","orcid":"https://orcid.org/0000-0001-7672-0942","observed_orcids":["https://orcid.org/0000-0001-7672-0942"]},"institutions":[{"id":"https://openalex.org/I1289490764","display_name":"Centers for Disease Control and Prevention","ror":"https://ror.org/042twtr12","country_code":"US","type":"government","lineage":["https://openalex.org/I1289490764","https://openalex.org/I1299022934"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H Moura","raw_affiliation_strings":["Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341-3724, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341-3724, USA","institution_ids":["https://openalex.org/I1289490764"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040276233","display_name":"David Asa Schwartz","orcid":"https://orcid.org/0000-0002-7486-8545","observed_orcids":["https://orcid.org/0000-0002-7486-8545"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D A Schwartz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018644477","display_name":"Fernando Jorge Bornay-Llinares","orcid":"https://orcid.org/0000-0002-1846-1943","observed_orcids":["https://orcid.org/0000-0002-1846-1943"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"F Bornay-Llinares","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104273426","display_name":"Fernando Campos Sodr\u00e9","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"F C Sodr\u00e9","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113938996","display_name":"S Wallace","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Wallace","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111816256","display_name":"GOVINDA S. VISVESVARA","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G S Visvesvara","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.2071,"has_fulltext":false,"cited_by_count":83,"citation_normalized_percentile":{"value":0.93730516,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"121","issue":"8","first_page":"888","last_page":"93"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11086","display_name":"Parasitic Infections and Diagnostics","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11086","display_name":"Parasitic Infections and Diagnostics","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13921","display_name":"Biological Stains and Phytochemicals","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microsporidia","display_name":"microsporidia","score":0.8700000047683716},{"id":"https://openalex.org/keywords/crystal-violet","display_name":"crystal violet","score":0.5370000004768372},{"id":"https://openalex.org/keywords/iodine","display_name":"iodine","score":0.8730000257492065},{"id":"https://openalex.org/keywords/chromotrope-2r","display_name":"chromotrope 2R","score":0.9419999718666077},{"id":"https://openalex.org/keywords/fast-green","display_name":"fast green","score":0.9570000171661377},{"id":"https://openalex.org/keywords/phosphotungstic-acid","display_name":"phosphotungstic acid","score":0.9950000047683716},{"id":"https://openalex.org/keywords/paraffin-sections","display_name":"paraffin sections","score":0.75},{"id":"https://openalex.org/keywords/feces","display_name":"feces","score":0.3190000057220459},{"id":"https://openalex.org/keywords/bronchoalveolar-lavage","display_name":"bronchoalveolar lavage","score":0.9760000109672546},{"id":"https://openalex.org/keywords/rapid-staining-technique","display_name":"rapid staining technique","score":0.4020000100135803}],"concepts":[{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.793830156326294},{"id":"https://openalex.org/C205501099","wikidata":"https://www.wikidata.org/wiki/Q188990","display_name":"Gram staining","level":3,"score":0.712807834148407},{"id":"https://openalex.org/C2781294515","wikidata":"https://www.wikidata.org/wiki/Q2733470","display_name":"Stain","level":3,"score":0.6344791650772095},{"id":"https://openalex.org/C2776020440","wikidata":"https://www.wikidata.org/wiki/Q420705","display_name":"Crystal violet","level":2,"score":0.5345999598503113},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46123266220092773},{"id":"https://openalex.org/C174618031","wikidata":"https://www.wikidata.org/wiki/Q177332","display_name":"Spore","level":2,"score":0.4544064402580261},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.4155261516571045},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.35690009593963623},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1821882724761963},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001826","descriptor_name":"Body Fluids","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":false},{"descriptor_ui":"D005243","descriptor_name":"Feces","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":false},{"descriptor_ui":"D005840","descriptor_name":"Gentian Violet","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007411","descriptor_name":"Intestinal Diseases, Parasitic","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D010619","descriptor_name":"Phenazines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013170","descriptor_name":"Spores","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016612","descriptor_name":"Paraffin Embedding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016816","descriptor_name":"Microsporida","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D016816","descriptor_name":"Microsporida","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D016816","descriptor_name":"Microsporida","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016881","descriptor_name":"Microsporidiosis","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:9278620","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9278620","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of pathology & laboratory medicine","raw_type":"Journal Article"},{"id":"mag:200085130","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9278620","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4000000059604645,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1995619061","https://openalex.org/W1503005203","https://openalex.org/W2015783760","https://openalex.org/W2413900019","https://openalex.org/W2189527634","https://openalex.org/W7100508889","https://openalex.org/W1829823590","https://openalex.org/W2417817837","https://openalex.org/W2158811536","https://openalex.org/W29634841"],"abstract_inverted_index":{"OBJECTIVE:":[0],"This":[1,127],"report":[2],"describes":[3],"a":[4,68,112],"new":[5,103,128],"and":[6,26,35,60,93,101,116,133],"improved":[7],"\"quick-hot":[8],"Gram-chromotrope\"":[9],"staining":[10,119],"technique":[11,129],"that":[12],"detects":[13],"microsporidian":[14,105],"spores":[15,106],"in":[16,33,47,63,142],"clinical":[17,143],"specimens,":[18],"such":[19],"as":[20,30,32],"stool,":[21],"urine,":[22],"saliva,":[23],"nasopharyngeal":[24],"fluid,":[25],"bronchoalveolar":[27],"lavage":[28],"samples,":[29],"well":[31],"formalin-fixed":[34],"paraffin-embedded":[36],"tissue":[37],"sections.":[38],"DESIGN:":[39],"In":[40],"this":[41,99],"procedure,":[42],"the":[43,102,117],"samples":[44],"are":[45,107],"stained":[46,108],"heated":[48],"(50":[49],"degrees":[50,54,75,79],"C":[51,76],"to":[52,73,77,123],"55":[53,78],"C)":[55],"solutions":[56],"of":[57,86],"crystal":[58],"violet":[59,110],"iodine":[61],"used":[62],"Gram's":[64],"stain,":[65],"followed":[66],"by":[67],"modified":[69,82],"chromotrope":[70,87],"solution":[71],"(heated":[72],"50":[74],"C).":[80],"The":[81],"stain":[83,100],"is":[84,121,130],"composed":[85],"2R":[88],"(1%),":[89],"fast":[90],"green":[91,114],"(0.15%),":[92],"phosphotungstic":[94],"acid":[95],"(0.25%).":[96],"RESULTS:":[97],"With":[98],"protocol,":[104],"dark":[109],"against":[111],"pale":[113],"background,":[115],"total":[118],"time":[120],"shortened":[122],"5":[124],"minutes.":[125],"CONCLUSIONS:":[126],"fast,":[131],"reliable,":[132],"simple.":[134],"It":[135],"can":[136],"be":[137],"easily":[138],"adapted":[139],"for":[140],"use":[141],"laboratories.":[144]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
