{"id":"https://openalex.org/W2000837396","doi":"https://doi.org/10.1089/107999099313622","title":"Intracellular Analysis of Interleukin-2 Induction Provides Direct Evidence at the Single Cell Level of Differential Coactivation Requirements for CD45RA+ and CD45RO+ T Cell Subsets","display_name":"Intracellular Analysis of Interleukin-2 Induction Provides Direct Evidence at the Single Cell Level of Differential Coactivation Requirements for CD45RA+ and CD45RO+ T Cell Subsets","publication_year":1999,"publication_date":"1999-07-01","ids":{"openalex":"https://openalex.org/W2000837396","doi":"https://doi.org/10.1089/107999099313622","mag":"2000837396","pmid":"https://pubmed.ncbi.nlm.nih.gov/10454348"},"language":"en","primary_location":{"id":"doi:10.1089/107999099313622","doi":"https://doi.org/10.1089/107999099313622","is_oa":false,"landing_page_url":"https://doi.org/10.1089/107999099313622","pdf_url":null,"source":{"id":"https://openalex.org/S109620152","display_name":"Journal of Interferon & Cytokine Research","issn_l":"1079-9907","issn":["1079-9907","1557-7465"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Interferon & Cytokine Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075469665","display_name":"Darren G. Woodside","orcid":"https://orcid.org/0000-0002-8521-6593","observed_orcids":["https://orcid.org/0000-0002-8521-6593"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]},{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Darren G. Woodside","raw_affiliation_strings":["University of Texas M.D. Anderson Cancer Center, Houston 77030, USA","Scripps Research Institute#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Texas M.D. Anderson Cancer Center, Houston 77030, USA","institution_ids":["https://openalex.org/I1343551460"]},{"raw_affiliation_string":"Scripps Research Institute#TAB#","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009822850","display_name":"Dorothy A. Long","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I919571938","display_name":"The University of Texas Health Science Center at Houston","ror":"https://ror.org/03gds6c39","country_code":"US","type":"education","lineage":["https://openalex.org/I919571938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dorothy A. Long","raw_affiliation_strings":["","University of Texas Health Science Center at Houston"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]},{"raw_affiliation_string":"University of Texas Health Science Center at Houston","institution_ids":["https://openalex.org/I919571938"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5042878383","display_name":"Bradley W. McIntyre","orcid":"https://orcid.org/0000-0002-1042-4064","observed_orcids":["https://orcid.org/0000-0002-1042-4064"]},"institutions":[{"id":"https://openalex.org/I919571938","display_name":"The University of Texas Health Science Center at Houston","ror":"https://ror.org/03gds6c39","country_code":"US","type":"education","lineage":["https://openalex.org/I919571938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bradley W. Mcintyre","raw_affiliation_strings":["","University of Texas Health Science Center at Houston"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]},{"raw_affiliation_string":"University of Texas Health Science Center at Houston","institution_ids":["https://openalex.org/I919571938"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3421,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.54652288,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"19","issue":"7","first_page":"769","last_page":"779"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10469","display_name":"Psoriasis: Treatment and Pathogenesis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/interleukin-2","display_name":"interleukin-2","score":0.8889999985694885},{"id":"https://openalex.org/keywords/cd45ra-naive-t-cells","display_name":"CD45RA+ naive T cells","score":0.9110000133514404},{"id":"https://openalex.org/keywords/cd45ro-t-cells","display_name":"CD45RO+ memory T cells","score":1.0},{"id":"https://openalex.org/keywords/cd28-costimulation","display_name":"CD28 costimulation","score":0.9259999990463257},{"id":"https://openalex.org/keywords/integrins","display_name":"integrins","score":0.7149999737739563},{"id":"https://openalex.org/keywords/cd26","display_name":"CD26","score":0.8930000066757202},{"id":"https://openalex.org/keywords/cd43","display_name":"CD43","score":0.9359999895095825},{"id":"https://openalex.org/keywords/cd44","display_name":"CD44","score":0.9990000128746033},{"id":"https://openalex.org/keywords/tcr-cd3-complex","display_name":"TCR/CD3 complex","score":0.8989999890327454},{"id":"https://openalex.org/keywords/t-cell-proliferation","display_name":"T cell proliferation","score":0.7870000004768372},{"id":"https://openalex.org/keywords/peripheral-blood-t-cells","display_name":"peripheral blood T cells","score":0.7160000205039978}],"concepts":[{"id":"https://openalex.org/C148125776","wikidata":"https://www.wikidata.org/wiki/Q1622348","display_name":"CD28","level":4,"score":0.8288053274154663},{"id":"https://openalex.org/C79484868","wikidata":"https://www.wikidata.org/wiki/Q5971673","display_name":"IL-2 receptor","level":4,"score":0.6692638993263245},{"id":"https://openalex.org/C2775982045","wikidata":"https://www.wikidata.org/wiki/Q6959842","display_name":"Naive T cell","level":5,"score":0.6577282547950745},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.6554843783378601},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5527252554893494},{"id":"https://openalex.org/C2777371288","wikidata":"https://www.wikidata.org/wiki/Q410130","display_name":"Interleukin 2","level":3,"score":0.48565638065338135},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.46937990188598633},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4458179175853729},{"id":"https://openalex.org/C2908885563","wikidata":"https://www.wikidata.org/wiki/Q410130","display_name":"Aldesleukin","level":4,"score":0.43435797095298767},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.4334942698478699},{"id":"https://openalex.org/C2778690821","wikidata":"https://www.wikidata.org/wiki/Q212354","display_name":"Cytokine","level":2,"score":0.4230918288230896},{"id":"https://openalex.org/C2777367678","wikidata":"https://www.wikidata.org/wiki/Q2304808","display_name":"Memory T cell","level":4,"score":0.4223366379737854},{"id":"https://openalex.org/C19317047","wikidata":"https://www.wikidata.org/wiki/Q412037","display_name":"T-cell receptor","level":4,"score":0.41687777638435364},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.41524431109428406},{"id":"https://openalex.org/C2780060146","wikidata":"https://www.wikidata.org/wiki/Q637488","display_name":"Coactivation","level":3,"score":0.4104151725769043},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36955082416534424},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3506656289100647},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.15847691893577576},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14859461784362793},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1274106800556183},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.06572455167770386},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0},{"id":"https://openalex.org/C2777515770","wikidata":"https://www.wikidata.org/wiki/Q507369","display_name":"Electromyography","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007156","descriptor_name":"Immunologic Memory","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007376","descriptor_name":"Interleukin-2","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D008213","descriptor_name":"Lymphocyte Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012016","descriptor_name":"Reference Values","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012995","descriptor_name":"Solubility","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016176","descriptor_name":"T-Lymphocyte Subsets","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D017493","descriptor_name":"Leukocyte Common Antigens","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1089/107999099313622","doi":"https://doi.org/10.1089/107999099313622","is_oa":false,"landing_page_url":"https://doi.org/10.1089/107999099313622","pdf_url":null,"source":{"id":"https://openalex.org/S109620152","display_name":"Journal of Interferon & Cytokine Research","issn_l":"1079-9907","issn":["1079-9907","1557-7465"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Interferon & Cytokine Research","raw_type":"journal-article"},{"id":"pmid:10454348","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10454348","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G247789771","display_name":"ADHESION MOLECULES IN HUMAN LEUKEMIA AND LYMPHOMA","funder_award_id":"5r01ca062596-07","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G799044023","display_name":null,"funder_award_id":"CA62596","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":54,"referenced_works":["https://openalex.org/W101029459","https://openalex.org/W1469469324","https://openalex.org/W1485596855","https://openalex.org/W1492908069","https://openalex.org/W1503486947","https://openalex.org/W1513713966","https://openalex.org/W1516808533","https://openalex.org/W1523829475","https://openalex.org/W1533770469","https://openalex.org/W1543510138","https://openalex.org/W1562057469","https://openalex.org/W1565814966","https://openalex.org/W1569428681","https://openalex.org/W1571284682","https://openalex.org/W1578883193","https://openalex.org/W1598532102","https://openalex.org/W1599302006","https://openalex.org/W1879927001","https://openalex.org/W1912833239","https://openalex.org/W1968092964","https://openalex.org/W1969931271","https://openalex.org/W1978212737","https://openalex.org/W1992635200","https://openalex.org/W1999481925","https://openalex.org/W2008106258","https://openalex.org/W2019047500","https://openalex.org/W2028146382","https://openalex.org/W2043326389","https://openalex.org/W2044592750","https://openalex.org/W2048073154","https://openalex.org/W2060901912","https://openalex.org/W2062058886","https://openalex.org/W2065051915","https://openalex.org/W2072782448","https://openalex.org/W2075772275","https://openalex.org/W2081936911","https://openalex.org/W2105807629","https://openalex.org/W2108336145","https://openalex.org/W2109161906","https://openalex.org/W2109830819","https://openalex.org/W2112625426","https://openalex.org/W2116195930","https://openalex.org/W2133811759","https://openalex.org/W2139229993","https://openalex.org/W2142351871","https://openalex.org/W2148517786","https://openalex.org/W2155782151","https://openalex.org/W2162856391","https://openalex.org/W2169318446","https://openalex.org/W2172383460","https://openalex.org/W2172960056","https://openalex.org/W2994404002","https://openalex.org/W3094779018","https://openalex.org/W4248034314"],"related_works":["https://openalex.org/W2146646284","https://openalex.org/W7099574596","https://openalex.org/W1969931271","https://openalex.org/W2037672703","https://openalex.org/W1933255801","https://openalex.org/W2414710475","https://openalex.org/W2091391876","https://openalex.org/W2025730147","https://openalex.org/W1522355179","https://openalex.org/W4313305990"],"abstract_inverted_index":{"Through":[0],"measurements":[1],"of":[2,38,76,99,107,131,147,164,183,189,199],"intracellular":[3],"cytokine":[4],"production,":[5],"evidence":[6,156],"is":[7,170],"provided":[8],"at":[9],"the":[10,53,96,105,108,128,139,148],"single":[11,159],"cell":[12,17,28,31,142,160,203],"level":[13,161],"that":[14,87,162],"triggering":[15],"different":[16,149,200],"surface":[18,204],"molecules":[19,40],"preferentially":[20,56],"activates":[21],"discrete":[22],"human":[23,165],"peripheral":[24],"blood":[25],"(PB)":[26],"T":[27,30,60,141,168,192,202],"subsets.":[29],"costimulation":[32,180],"due":[33,88,126],"to":[34,89,127,178],"cross-linking":[35],"a":[36,158,197],"variety":[37,198],"individual":[39],"(beta1,":[41],"beta2,":[42],"and":[43,71],"beta7":[44],"integrins,":[45],"CD26,":[46],"CD43,":[47],"or":[48,91],"CD44),":[49],"in":[50,68,104,124,174],"conjunction":[51],"with":[52],"CD3/TCR":[54],"complex,":[55],"activated":[57,144],"CD45RO+":[58,70,190],"PB":[59,201],"lymphocytes.":[61],"CD28,":[62],"however,":[63],"costimulated":[64],"interleukin-2":[65],"(IL-2)":[66],"production":[67],"both":[69],"CD45RA+":[72,166],"subpopulations.":[73],"The":[74,112,152],"amount":[75,98],"soluble":[77,100],"IL-2":[78,101,110,132,184],"produced":[79,102],"by":[80,196],"CD28":[81,179],"coactivation":[82,188],"was":[83,103,123],"15-30-fold":[84],"higher":[85],"than":[86],"integrin":[90],"CD26-dependent":[92],"coactivation,":[93],"although":[94],"even":[95],"lowest":[97],"range":[106],"high-affinity":[109],"receptor.":[111],"overall":[113],"proliferative":[114],"responses":[115],"were":[116],"similar":[117],"among":[118],"all":[119],"costimulatory":[120,150],"settings.":[121],"This":[122],"part":[125],"uniform":[129],"upregulation":[130],"receptor-alpha":[133],"(IL-2R":[134],"alpha)":[135],"(CD25)":[136],"expression":[137],"on":[138,157],"entire":[140],"population":[143],"under":[145],"each":[146],"conditions.":[151],"data":[153],"provide":[154],"direct":[155],"activation":[163],"(naive)":[167],"cells":[169],"stringently":[171],"controlled":[172],"and,":[173],"these":[175],"studies,":[176],"limited":[177],"for":[181],"induction":[182],"production.":[185],"In":[186],"contrast,":[187],"(memory)":[191],"lymphocytes":[193],"can":[194],"proceed":[195],"molecules.":[205]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}