{"id":"https://openalex.org/W2000698860","doi":"https://doi.org/10.47371/mycosci.myc42479","title":"Purification and characterization of an extracellular acid trehalase from Lentinula edodes","display_name":"Purification and characterization of an extracellular acid trehalase from Lentinula edodes","publication_year":2001,"publication_date":"2001-01-01","ids":{"openalex":"https://openalex.org/W2000698860","doi":"https://doi.org/10.47371/mycosci.myc42479","mag":"2000698860"},"language":"en","primary_location":{"id":"doi:10.47371/mycosci.myc42479","doi":"https://doi.org/10.47371/mycosci.myc42479","is_oa":false,"landing_page_url":"https://doi.org/10.47371/mycosci.myc42479","pdf_url":null,"source":{"id":"https://openalex.org/S85891262","display_name":"Mycoscience","issn_l":"1340-3540","issn":["1340-3540","1618-2545"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mycoscience","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019264033","display_name":"Mika Murata","orcid":"https://orcid.org/0000-0001-8155-0335","observed_orcids":["https://orcid.org/0000-0001-8155-0335"]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Mika Murata","raw_affiliation_strings":["Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","institution_ids":["https://openalex.org/I916559398"]},{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102735642","display_name":"Masaru Nagai","orcid":"https://orcid.org/0000-0002-1373-7322","observed_orcids":["https://orcid.org/0000-0002-1373-7322"]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Masaru Nagai","raw_affiliation_strings":["Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","institution_ids":["https://openalex.org/I916559398"]},{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076400522","display_name":"Makoto Takao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Makoto Takao","raw_affiliation_strings":["Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","institution_ids":["https://openalex.org/I916559398"]},{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006300612","display_name":"Akira Suzuki","orcid":"https://orcid.org/0000-0002-5950-8808","observed_orcids":["https://orcid.org/0000-0002-5950-8808"]},"institutions":[{"id":"https://openalex.org/I159385669","display_name":"Chiba University","ror":"https://ror.org/01hjzeq58","country_code":"JP","type":"education","lineage":["https://openalex.org/I159385669"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Akira Suzuki","raw_affiliation_strings":["Faculty of Education, Chiba University, 1\u201333 Yayoicho, Inageku, Chiba 263-8522, Japan","Faculty of Education, Chiba University, Chiba, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Education, Chiba University, 1\u201333 Yayoicho, Inageku, Chiba 263-8522, Japan","institution_ids":["https://openalex.org/I159385669"]},{"raw_affiliation_string":"Faculty of Education, Chiba University, Chiba, Japan","institution_ids":["https://openalex.org/I159385669"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108405694","display_name":"Takuo Sakai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takuo Sakai","raw_affiliation_strings":["Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","institution_ids":["https://openalex.org/I916559398"]},{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan","institution_ids":["https://openalex.org/I916559398"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5074242588","display_name":"Takao Terashita","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I916559398","display_name":"Kindai University","ror":"https://ror.org/05kt9ap64","country_code":"JP","type":"education","lineage":["https://openalex.org/I916559398"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takao Terashita","raw_affiliation_strings":["Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, 3327-204 Nakamachi, Nara 631-8505, Japan","institution_ids":["https://openalex.org/I916559398"]},{"raw_affiliation_string":"Laboratory of Food Microbiology, Faculty of Agriculture, Kin-ki University, Nara, Japan","institution_ids":["https://openalex.org/I916559398"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":3000},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"42","issue":"5","first_page":"479","last_page":"482"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9628999829292297,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9628999829292297,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11085","display_name":"Fungal Biology and Applications","score":0.012400000356137753,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.006099999882280827,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trehalase","display_name":"trehalase","score":0.8209999799728394},{"id":"https://openalex.org/keywords/lentinula-edodes","display_name":"shiitake mushroom","score":0.8859999775886536},{"id":"https://openalex.org/keywords/acid-trehalase","display_name":"acid trehalase","score":0.859000027179718},{"id":"https://openalex.org/keywords/extracellular-enzymes","display_name":"extracellular enzymes","score":0.8939999938011169},{"id":"https://openalex.org/keywords/trehalose","display_name":"trehalose","score":0.7310000061988831},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.8370000123977661},{"id":"https://openalex.org/keywords/enzyme-characterization","display_name":"enzyme characterization","score":0.8029999732971191},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.5460000038146973},{"id":"https://openalex.org/keywords/ph-stability","display_name":"pH stability","score":0.6729999780654907},{"id":"https://openalex.org/keywords/fungal-culture-filtrate","display_name":"fungal culture filtrate","score":0.5059999823570251}],"concepts":[{"id":"https://openalex.org/C2776236347","wikidata":"https://www.wikidata.org/wiki/Q1303903","display_name":"Lentinula","level":3,"score":0.954023003578186},{"id":"https://openalex.org/C2779315325","wikidata":"https://www.wikidata.org/wiki/Q410630","display_name":"Trehalase","level":3,"score":0.9498851299285889},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8361105918884277},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.5463980436325073},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4830268323421478},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.4689369201660156},{"id":"https://openalex.org/C2992763725","wikidata":"https://www.wikidata.org/wiki/Q99974","display_name":"Mycorrhizal fungi","level":3,"score":0.45846566557884216},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3609852194786072},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.25116366147994995},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.0663628876209259},{"id":"https://openalex.org/C180032290","wikidata":"https://www.wikidata.org/wiki/Q1664296","display_name":"Inoculation","level":2,"score":0.0},{"id":"https://openalex.org/C2776474821","wikidata":"https://www.wikidata.org/wiki/Q83093","display_name":"Mushroom","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.47371/mycosci.myc42479","doi":"https://doi.org/10.47371/mycosci.myc42479","is_oa":false,"landing_page_url":"https://doi.org/10.47371/mycosci.myc42479","pdf_url":null,"source":{"id":"https://openalex.org/S85891262","display_name":"Mycoscience","issn_l":"1340-3540","issn":["1340-3540","1618-2545"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mycoscience","raw_type":"journal-article"},{"id":"doi:10.1007/bf02464344","doi":"https://doi.org/10.1007/bf02464344","is_oa":false,"landing_page_url":"https://doi.org/10.1007/bf02464344","pdf_url":null,"source":{"id":"https://openalex.org/S85891262","display_name":"Mycoscience","issn_l":"1340-3540","issn":["1340-3540","1618-2545"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mycoscience","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W197242203","https://openalex.org/W346714302","https://openalex.org/W1533043270","https://openalex.org/W1568028271","https://openalex.org/W1775749144","https://openalex.org/W1978255313","https://openalex.org/W1985070028","https://openalex.org/W1989607812","https://openalex.org/W1991300428","https://openalex.org/W2039924783","https://openalex.org/W2048946374","https://openalex.org/W2058155957","https://openalex.org/W2071252308","https://openalex.org/W2072440127","https://openalex.org/W2093229425","https://openalex.org/W2100837269","https://openalex.org/W2153965302","https://openalex.org/W2444617614","https://openalex.org/W3155682722","https://openalex.org/W4231121696"],"related_works":["https://openalex.org/W2054900203","https://openalex.org/W1484231629","https://openalex.org/W2039924783","https://openalex.org/W2058155957","https://openalex.org/W7215108923","https://openalex.org/W2382737293","https://openalex.org/W2156636217","https://openalex.org/W2095539791","https://openalex.org/W2275480276","https://openalex.org/W122997310"],"abstract_inverted_index":{"Trehalase":[0],"from":[1],"the":[2,29],"culture":[3],"filtrate":[4],"of":[5,37,71,79],"Lentinula":[6],"edodes":[7],"was":[8,35,61,90],"purified":[9],"and":[10,20,26,41,48,55,66,89],"characterized.":[11],"Molecular":[12],"masses":[13],"were":[14,50],"estimated":[15],"to":[16,64],"be":[17],"158":[18],"kDa":[19,22],"79-91":[21],"by":[23],"gel":[24],"filtration":[25],"SDS-PAGE":[27],"under":[28],"reduced":[30],"condition,":[31],"respectively.":[32,58],"The":[33,45,59],"enzyme":[34,60],"composed":[36],"two":[38],"identical":[39],"subunits":[40],"contained":[42],"carbohydrate":[43],"molecules.":[44],"optimum":[46],"temperature":[47],"pH":[49,56,70],"obtained":[51],"at":[52,73],"around":[53],"40\u00b0C":[54,65],"5.0,":[57],"stable":[62],"up":[63],"in":[67],"a":[68],"range":[69],"4-10":[72],"30\u00b0C":[74],"It":[75],"cleaved":[76],"a-1,1":[77],"linkages":[78],"trehalose,":[80],"but":[81],"not":[82],"\u03b1-1,4,":[83],"\u03b1-1,6":[84],"or":[85],"\u03b2-1,4":[86],"glycosyl":[87],"linkages,":[88],"defined":[91],"as":[92],"an":[93],"acid":[94],"trehalase.":[95]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}