{"id":"https://openalex.org/W2000630129","doi":"https://doi.org/10.3109/00498259509046663","title":"Determination of P4501A2 activity in human liver microsomes using [3-14C-methyl]caffeine","display_name":"Determination of P4501A2 activity in human liver microsomes using [3-14C-methyl]caffeine","publication_year":1995,"publication_date":"1995-01-01","ids":{"openalex":"https://openalex.org/W2000630129","doi":"https://doi.org/10.3109/00498259509046663","mag":"2000630129","pmid":"https://pubmed.ncbi.nlm.nih.gov/8553685"},"language":"en","primary_location":{"id":"doi:10.3109/00498259509046663","doi":"https://doi.org/10.3109/00498259509046663","is_oa":false,"landing_page_url":"https://doi.org/10.3109/00498259509046663","pdf_url":null,"source":{"id":"https://openalex.org/S48760969","display_name":"Xenobiotica","issn_l":"0049-8254","issn":["0049-8254","1366-5928"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Xenobiotica","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108216286","display_name":"J. C. Bloomer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J. C. Bloomer","raw_affiliation_strings":["Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, Frythe, Welwyn, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, Frythe, Welwyn, UK","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111977379","display_name":"S. E. Clarke","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S. E. Clarke","raw_affiliation_strings":["Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, The Frythe, Welwyn, AL6 9AR, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, The Frythe, Welwyn, AL6 9AR, UK","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111628263","display_name":"Richard J. Chenery","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R. J. Chenery","raw_affiliation_strings":["Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, The Frythe, Welwyn, AL6 9AR, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, The Frythe, Welwyn, AL6 9AR, UK","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.4616,"has_fulltext":false,"cited_by_count":26,"citation_normalized_percentile":{"value":0.79648104,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"25","issue":"9","first_page":"917","last_page":"927"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11264","display_name":"Coffee research and impacts","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11465","display_name":"Drug-Induced Hepatotoxicity and Protection","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cyp1a2","display_name":"CYP1A2","score":0.9430000185966492},{"id":"https://openalex.org/keywords/caffeine-metabolism","display_name":"caffeine metabolism","score":0.7760000228881836},{"id":"https://openalex.org/keywords/human-liver-microsomes","display_name":"human liver microsomes","score":0.890999972820282},{"id":"https://openalex.org/keywords/solid-phase-extraction","display_name":"solid-phase extraction","score":0.7549999952316284},{"id":"https://openalex.org/keywords/enzyme-activity-assay","display_name":"enzyme activity assay","score":0.5809999704360962}],"concepts":[{"id":"https://openalex.org/C2777379063","wikidata":"https://www.wikidata.org/wiki/Q161233","display_name":"Formic acid","level":2,"score":0.7983897924423218},{"id":"https://openalex.org/C2775930285","wikidata":"https://www.wikidata.org/wiki/Q161210","display_name":"Formaldehyde","level":2,"score":0.7970589995384216},{"id":"https://openalex.org/C202199734","wikidata":"https://www.wikidata.org/wiki/Q900386","display_name":"Demethylation","level":5,"score":0.7952666282653809},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7842552661895752},{"id":"https://openalex.org/C87644729","wikidata":"https://www.wikidata.org/wiki/Q547502","display_name":"Microsome","level":3,"score":0.7583853006362915},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7195427417755127},{"id":"https://openalex.org/C2778500429","wikidata":"https://www.wikidata.org/wiki/Q60235","display_name":"Caffeine","level":2,"score":0.6848379373550415},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.40316247940063477},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39933642745018005},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.09372201561927795},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.05077454447746277},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.0},{"id":"https://openalex.org/C190727270","wikidata":"https://www.wikidata.org/wiki/Q874745","display_name":"DNA methylation","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D002110","descriptor_name":"Caffeine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D002250","descriptor_name":"Carbon Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003577","descriptor_name":"Cytochrome P-450 Enzyme System","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D005557","descriptor_name":"Formaldehyde","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005561","descriptor_name":"Formates","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008862","descriptor_name":"Microsomes, Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D011869","descriptor_name":"Radioligand Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019388","descriptor_name":"Cytochrome P-450 CYP1A2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.3109/00498259509046663","doi":"https://doi.org/10.3109/00498259509046663","is_oa":false,"landing_page_url":"https://doi.org/10.3109/00498259509046663","pdf_url":null,"source":{"id":"https://openalex.org/S48760969","display_name":"Xenobiotica","issn_l":"0049-8254","issn":["0049-8254","1366-5928"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Xenobiotica","raw_type":"journal-article"},{"id":"pmid:8553685","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8553685","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Xenobiotica; the fate of foreign compounds in biological systems","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1544140936","https://openalex.org/W1577017476","https://openalex.org/W1969652902","https://openalex.org/W1979610724","https://openalex.org/W1996968257","https://openalex.org/W1997567581","https://openalex.org/W2014884679","https://openalex.org/W2017729229","https://openalex.org/W2025014579","https://openalex.org/W2063968553","https://openalex.org/W2068662808","https://openalex.org/W2068847386","https://openalex.org/W2086104648","https://openalex.org/W2088342692","https://openalex.org/W2093529618","https://openalex.org/W2105876917","https://openalex.org/W2150946709","https://openalex.org/W2232261217","https://openalex.org/W2304131176","https://openalex.org/W2318537062","https://openalex.org/W2400809234","https://openalex.org/W4232534952","https://openalex.org/W4238918924","https://openalex.org/W4244098017"],"related_works":["https://openalex.org/W2030595001","https://openalex.org/W2093529618","https://openalex.org/W2338498280","https://openalex.org/W2012863453","https://openalex.org/W7101012425","https://openalex.org/W2089626723","https://openalex.org/W2090946743","https://openalex.org/W2308033060","https://openalex.org/W2011269087","https://openalex.org/W2135536512"],"abstract_inverted_index":{"1.":[0],"Caffeine":[1],"N3-demethylation,":[2],"the":[3,18,49,85],"major":[4],"pathway":[5],"of":[6,17,55,67,87,117,157],"caffeine":[7,88,138],"metabolism":[8],"in":[9,29,37,112,160,168,169],"man,":[10],"is":[11,24,164],"mediated":[12],"by":[13,91,134,142],"P4501A2.":[14],"The":[15,108],"carbon":[16,27],"methyl":[19],"group":[20],"lost":[21],"during":[22],"N3-demethylation":[23,86,109,139],"eliminated":[25],"as":[26,32,174],"dioxide":[28],"vivo,":[30],"or":[31],"formaldehyde":[33],"and":[34,42,75,82,100,129],"formic":[35],"acid":[36,51,69,159],"vitro.":[38],"2.":[39],"A":[40],"simple":[41],"sensitive":[43],"assay":[44,93],"was":[45,130,140],"developed":[46],"to":[47,94],"quantify":[48],"[14C]formaldehyde/[14C]formic":[50,68,158],"produced":[52],"following":[53],"incubation":[54],"human":[56,118,149,161],"microsomes":[57,113,143],"with":[58,70,122,148],"[3-14C-methyl]caffeine.":[59],"This":[60,153],"assay,":[61,154],"using":[62],"solid-phase":[63],"extraction,":[64],"enables":[65],"quantitation":[66,156],"acceptable":[71],"precision":[72],"(within":[73,77],"5%)":[74],"accuracy":[76],"10%).":[78],"3.":[79],"Typical":[80],"Km":[81],"Vmax":[83],"for":[84,155,166,177],"were":[89],"determined":[90,111],"this":[92],"be":[95],"500":[96],"(range":[97,102],"220-1200)":[98],"microM,":[99],"250":[101],"115-450)":[103],"pmol.mg":[104],"protein-1.min-1":[105],"respectively.":[106],"4.":[107],"activity":[110],"from":[114,144],"a":[115,175],"range":[116],"livers":[119],"correlated":[120],"significantly":[121],"other":[123],"P4501A2":[124,150,178],"activities":[125],"(p":[126],"<":[127],"0.001)":[128],"inhibited":[131],"(>":[132],"95%)":[133],"furafylline.":[135],"In":[136],"addition,":[137],"catalysed":[141],"cell":[145],"lines":[146],"transfected":[147],"cDNA.":[151],"5.":[152],"liver":[162],"microsomes,":[163],"suitable":[165],"use":[167],"vitro":[170],"drug":[171],"interaction":[172],"studies":[173],"probe":[176],"activity.":[179]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
