{"id":"https://openalex.org/W2000565662","doi":"https://doi.org/10.1034/j.1600-0560.2000.027005228.x","title":"Detection of TCR\u2010\u03b3 gene rearrangements in early mycosis fungoides by non\u2010radioactive PCR\u2010SSCP","display_name":"Detection of TCR\u2010\u03b3 gene rearrangements in early mycosis fungoides by non\u2010radioactive PCR\u2010SSCP","publication_year":2000,"publication_date":"2000-05-01","ids":{"openalex":"https://openalex.org/W2000565662","doi":"https://doi.org/10.1034/j.1600-0560.2000.027005228.x","mag":"2000565662","pmid":"https://pubmed.ncbi.nlm.nih.gov/10847547"},"language":"en","primary_location":{"id":"doi:10.1034/j.1600-0560.2000.027005228.x","doi":"https://doi.org/10.1034/j.1600-0560.2000.027005228.x","is_oa":false,"landing_page_url":"https://doi.org/10.1034/j.1600-0560.2000.027005228.x","pdf_url":null,"source":{"id":"https://openalex.org/S92442678","display_name":"Journal of Cutaneous Pathology","issn_l":"0303-6987","issn":["0303-6987","1600-0560"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cutaneous Pathology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101732121","display_name":"Michael J. Murphy","orcid":"https://orcid.org/0000-0002-4380-9323","observed_orcids":["https://orcid.org/0000-0002-4380-9323"]},"institutions":[{"id":"https://openalex.org/I77920804","display_name":"Albany Medical Center Hospital","ror":"https://ror.org/0307crw42","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4405259726","https://openalex.org/I77920804"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael Murphy","raw_affiliation_strings":["Department of Pathology and Laboratory Medicine, Albany Medical Center, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology and Laboratory Medicine, Albany Medical Center, New York, USA","institution_ids":["https://openalex.org/I77920804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046795494","display_name":"Sabina Signoretti","orcid":"https://orcid.org/0000-0002-5000-9105","observed_orcids":["https://orcid.org/0000-0002-5000-9105"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210117453","display_name":"Dana-Farber Cancer Institute","ror":"https://ror.org/02jzgtq86","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210117453"]},{"id":"https://openalex.org/I4210161994","display_name":"Dana-Farber Brigham Cancer Center","ror":"https://ror.org/05rgrbr06","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I4210117453","https://openalex.org/I4210161994","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sabina Signoretti","raw_affiliation_strings":["Department of Adult Oncology, Dana Farber Cancer Institute and Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Adult Oncology, Dana Farber Cancer Institute and Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1283280774","https://openalex.org/I136199984","https://openalex.org/I4210117453","https://openalex.org/I4210161994"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028359701","display_name":"Marshall E. Kadin","orcid":"https://orcid.org/0000-0003-1039-4384","observed_orcids":["https://orcid.org/0000-0003-1039-4384"]},"institutions":[{"id":"https://openalex.org/I1316535847","display_name":"Beth Israel Deaconess Medical Center","ror":"https://ror.org/04drvxt59","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316535847"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marshall E. Kadin","raw_affiliation_strings":["Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1316535847","https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5071763026","display_name":"Massimo F. Loda","orcid":"https://orcid.org/0000-0001-9674-8379","observed_orcids":["https://orcid.org/0000-0001-9674-8379"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210117453","display_name":"Dana-Farber Cancer Institute","ror":"https://ror.org/02jzgtq86","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210117453"]},{"id":"https://openalex.org/I4210161994","display_name":"Dana-Farber Brigham Cancer Center","ror":"https://ror.org/05rgrbr06","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I4210117453","https://openalex.org/I4210161994","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Massimo Loda","raw_affiliation_strings":["Department of Adult Oncology, Dana Farber Cancer Institute and Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Adult Oncology, Dana Farber Cancer Institute and Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1283280774","https://openalex.org/I136199984","https://openalex.org/I4210117453","https://openalex.org/I4210161994"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5071763026"],"corresponding_institution_ids":["https://openalex.org/I1283280774","https://openalex.org/I136199984","https://openalex.org/I4210117453","https://openalex.org/I4210161994"],"apc_list":{"value":3570,"currency":"USD","value_usd":3570},"apc_paid":null,"fwci":5.8201,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.9608834,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"27","issue":"5","first_page":"228","last_page":"234"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11811","display_name":"Cutaneous lymphoproliferative disorders research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2708","display_name":"Dermatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11811","display_name":"Cutaneous lymphoproliferative disorders research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2708","display_name":"Dermatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mycosis-fungoides","display_name":"mycosis fungoides","score":0.9869999885559082},{"id":"https://openalex.org/keywords/t-cell-receptor-gamma-gene-rearrangement","display_name":"T-cell receptor gamma gene rearrangement","score":0.8690000176429749},{"id":"https://openalex.org/keywords/t-cell-clonality","display_name":"T cell clonality","score":0.9390000104904175},{"id":"https://openalex.org/keywords/pcr-sscp","display_name":"PCR-SSCP","score":0.7730000019073486},{"id":"https://openalex.org/keywords/inflammatory-skin-diseases","display_name":"inflammatory skin diseases","score":0.8730000257492065},{"id":"https://openalex.org/keywords/differential-diagnosis","display_name":"differential diagnosis","score":0.609000027179718},{"id":"https://openalex.org/keywords/disease-recurrence-monitoring","display_name":"disease recurrence monitoring","score":0.4699999988079071}],"concepts":[{"id":"https://openalex.org/C2777756187","wikidata":"https://www.wikidata.org/wiki/Q1891209","display_name":"Mycosis fungoides","level":3,"score":0.9170911312103271},{"id":"https://openalex.org/C129219603","wikidata":"https://www.wikidata.org/wiki/Q1788683","display_name":"Single-strand conformation polymorphism","level":4,"score":0.8834587335586548},{"id":"https://openalex.org/C19317047","wikidata":"https://www.wikidata.org/wiki/Q412037","display_name":"T-cell receptor","level":4,"score":0.6787356734275818},{"id":"https://openalex.org/C2992511266","wikidata":"https://www.wikidata.org/wiki/Q1093098","display_name":"Gene rearrangement","level":3,"score":0.6651471853256226},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.6563754677772522},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.5513560175895691},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.514239490032196},{"id":"https://openalex.org/C2777891184","wikidata":"https://www.wikidata.org/wiki/Q7168693","display_name":"Peripheral T-cell lymphoma","level":4,"score":0.45925185084342957},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.38840731978416443},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.38420072197914124},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.3262938857078552},{"id":"https://openalex.org/C2779338263","wikidata":"https://www.wikidata.org/wiki/Q208414","display_name":"Lymphoma","level":2,"score":0.31225162744522095},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.30295529961586},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1752181053161621},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09052583575248718},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001706","descriptor_name":"Biopsy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009182","descriptor_name":"Mycosis Fungoides","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009182","descriptor_name":"Mycosis Fungoides","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D012008","descriptor_name":"Recurrence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012878","descriptor_name":"Skin Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012878","descriptor_name":"Skin Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D015334","descriptor_name":"Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016612","descriptor_name":"Paraffin Embedding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016692","descriptor_name":"Receptors, Antigen, T-Cell, gamma-delta","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018807","descriptor_name":"Polymorphism, Single-Stranded Conformational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1034/j.1600-0560.2000.027005228.x","doi":"https://doi.org/10.1034/j.1600-0560.2000.027005228.x","is_oa":false,"landing_page_url":"https://doi.org/10.1034/j.1600-0560.2000.027005228.x","pdf_url":null,"source":{"id":"https://openalex.org/S92442678","display_name":"Journal of Cutaneous Pathology","issn_l":"0303-6987","issn":["0303-6987","1600-0560"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cutaneous Pathology","raw_type":"journal-article"},{"id":"pmid:10847547","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10847547","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of cutaneous pathology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.98580002784729,"display_name":"Good health and well-being"}],"study_designs":[{"id":"https://openalex.org/study-designs/observational-study","display_name":"Observational Study"}],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2045475836","https://openalex.org/W7220348065","https://openalex.org/W1989169351","https://openalex.org/W2403443241","https://openalex.org/W2047563096","https://openalex.org/W2001729554","https://openalex.org/W2360994006","https://openalex.org/W2012704095","https://openalex.org/W2087415452","https://openalex.org/W2314948639"],"abstract_inverted_index":{"Early":[0],"mycosis":[1],"fungoides":[2],"(MF)":[3],"can":[4],"mimic":[5],"numerous":[6],"benign":[7],"inflammatory":[8,79,157],"dermatoses":[9,158],"on":[10],"routine":[11],"histological":[12],"examination.":[13],"In":[14,134],"this":[15],"study,":[16],"a":[17,49,164],"recently":[18],"developed":[19],"non-radioactive":[20,109,161],"polymerase":[21],"chain":[22],"reaction-single":[23],"strand":[24],"conformational":[25],"polymorphism":[26],"(PCR-SSCP)":[27],"technique":[28],"was":[29,60],"used":[30,96,197],"to":[31,198],"assess":[32],"T-cell":[33,98,151,172],"clonality":[34,173],"in":[35,117,141,155,174,202],"paraffin-embedded":[36,175],"skin":[37,64,76,143],"biopsies":[38,65,77,144],"clinically":[39],"and":[40,53,74,89,194],"pathologically":[41],"suspicious":[42,177],"for":[43,97,169,178,189],"early":[44,179],"MF.":[45,180],"Non-radioactive":[46],"PCR-SSCP":[47,162],"is":[48,163,187],"simple,":[50],"sensitive,":[51],"reproducible":[52],"rapid":[54],"procedure":[55],"requiring":[56],"minimal":[57],"instrumentation.":[58],"DNA":[59],"extracted":[61],"from":[62,145],"22":[63,120],"of":[66,78,119,171,184],"20":[67],"patients":[68],"with":[69],"suspected":[70,122],"patch":[71],"stage":[72],"MF":[73,123],"15":[75],"dermatoses.":[80],"V":[81,83,85,87],"gamma1-8,":[82],"gamma9,":[84],"gamma10,":[86],"gamma11":[88],"J":[90],"gamma1/J":[91],"gamma2":[92],"consensus":[93],"primers":[94],"were":[95,106,115,129,139,153],"receptor":[99],"(TCR)-gamma":[100],"gene":[101,113,192],"rearrangement":[102],"amplification.":[103],"PCR":[104,185],"products":[105,186],"analyzed":[107],"by":[108],"SSCP.":[110],"Clonal":[111,125],"TCR-gamma":[112,191],"rearrangements":[114],"detected":[116],"17":[118],"(77%)":[121],"specimens.":[124],"SSCP":[126,182],"banding":[127],"patterns":[128,138],"different":[130],"among":[131],"individual":[132,203],"patients.":[133,204],"addition,":[135],"identical":[136],"banded":[137],"demonstrated":[140],"serial":[142],"the":[146,156],"same":[147],"patient.":[148],"No":[149],"dominant":[150],"clones":[152],"found":[154],"studied.":[159],"Therefore,":[160],"useful":[165],"molecular":[166],"diagnostic":[167],"tool":[168],"assessment":[170],"specimens":[176],"The":[181],"imprint":[183],"specific":[188],"each":[190],"rearrangement,":[193],"may":[195],"be":[196],"evaluate":[199],"concurrent/recurrent":[200],"disease":[201]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}