{"id":"https://openalex.org/W2000508531","doi":"https://doi.org/10.1021/bi00588a037","title":"Suicide inactivation of the flavoenzyme D-lactate dehydrogenase by .alpha.-hydroxybutynoate","display_name":"Suicide inactivation of the flavoenzyme D-lactate dehydrogenase by .alpha.-hydroxybutynoate","publication_year":1979,"publication_date":"1979-10-16","ids":{"openalex":"https://openalex.org/W2000508531","doi":"https://doi.org/10.1021/bi00588a037","mag":"2000508531","pmid":"https://pubmed.ncbi.nlm.nih.gov/497163"},"language":"en","primary_location":{"id":"doi:10.1021/bi00588a037","doi":"https://doi.org/10.1021/bi00588a037","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00588a037","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://kops.uni-konstanz.de/server/api/core/bitstreams/74fa994a-6c50-4700-873a-375bd973bfd8/content","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055990354","display_name":"Steven T. Olson","orcid":"https://orcid.org/0000-0002-6083-7510","observed_orcids":["https://orcid.org/0000-0002-6083-7510"]},"institutions":[{"id":"https://openalex.org/I189712700","display_name":"University of Konstanz","ror":"https://ror.org/0546hnb39","country_code":"DE","type":"education","lineage":["https://openalex.org/I189712700"]},{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["DE","US"],"is_corresponding":false,"raw_author_name":"Steven T. Olson","raw_affiliation_strings":["Fachbereich Biologie der Universitat Konstanz, D775 Konstanz, West Germany","From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fachbereich Biologie der Universitat Konstanz, D775 Konstanz, West Germany","institution_ids":["https://openalex.org/I189712700"]},{"raw_affiliation_string":"From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079221179","display_name":"Vincent Massey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I189712700","display_name":"University of Konstanz","ror":"https://ror.org/0546hnb39","country_code":"DE","type":"education","lineage":["https://openalex.org/I189712700"]},{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["DE","US"],"is_corresponding":false,"raw_author_name":"Vincent Massey","raw_affiliation_strings":["Fachbereich Biologie der Universitat Konstanz, D775 Konstanz, West Germany","From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fachbereich Biologie der Universitat Konstanz, D775 Konstanz, West Germany","institution_ids":["https://openalex.org/I189712700"]},{"raw_affiliation_string":"From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089547236","display_name":"Sandro Ghisla","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I189712700","display_name":"University of Konstanz","ror":"https://ror.org/0546hnb39","country_code":"DE","type":"education","lineage":["https://openalex.org/I189712700"]},{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["DE","US"],"is_corresponding":false,"raw_author_name":"Sandro Ghisla","raw_affiliation_strings":["Fachbereich Biologie der Universitat Konstanz, D775 Konstanz, West Germany","From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fachbereich Biologie der Universitat Konstanz, D775 Konstanz, West Germany","institution_ids":["https://openalex.org/I189712700"]},{"raw_affiliation_string":"From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110900928","display_name":"Carolyn D. Whitfield","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I189712700","display_name":"University of Konstanz","ror":"https://ror.org/0546hnb39","country_code":"DE","type":"education","lineage":["https://openalex.org/I189712700"]},{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["DE","US"],"is_corresponding":false,"raw_author_name":"Carolyn D. Whitfield","raw_affiliation_strings":["Fachbereich Biologie der Universitat Konstanz, D775 Konstanz, West Germany","From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fachbereich Biologie der Universitat Konstanz, D775 Konstanz, West Germany","institution_ids":["https://openalex.org/I189712700"]},{"raw_affiliation_string":"From the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":3.9791,"has_fulltext":true,"cited_by_count":23,"citation_normalized_percentile":{"value":0.9339274,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":93},"biblio":{"volume":"18","issue":"21","first_page":"4724","last_page":"4732"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.7559000253677368,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.7559000253677368,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.09730000048875809,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12915","display_name":"Biochemical Acid Research Studies","score":0.020600000396370888,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/d-lactate-dehydrogenase","display_name":"D-lactate dehydrogenase","score":0.9929999709129333},{"id":"https://openalex.org/keywords/flavoenzyme","display_name":"flavoenzyme","score":0.7020000219345093},{"id":"https://openalex.org/keywords/irreversible-enzyme-inactivation","display_name":"irreversible enzyme inactivation","score":0.8100000023841858},{"id":"https://openalex.org/keywords/fad","display_name":"FAD","score":0.6909999847412109},{"id":"https://openalex.org/keywords/megasphaera-elsdenii","display_name":"Megasphaera elsdenii","score":0.8939999938011169},{"id":"https://openalex.org/keywords/substrate-inhibition","display_name":"substrate inhibition","score":0.3370000123977661},{"id":"https://openalex.org/keywords/keto-acids","display_name":"keto acids","score":0.39100000262260437}],"concepts":[{"id":"https://openalex.org/C2777318727","wikidata":"https://www.wikidata.org/wiki/Q409464","display_name":"Lactate dehydrogenase","level":3,"score":0.6840189695358276},{"id":"https://openalex.org/C64943373","wikidata":"https://www.wikidata.org/wiki/Q2651003","display_name":"Alpha (finance)","level":4,"score":0.6691258549690247},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5476478934288025},{"id":"https://openalex.org/C2909544887","wikidata":"https://www.wikidata.org/wiki/Q409464","display_name":"L-Lactate dehydrogenase","level":4,"score":0.485405296087265},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.45521965622901917},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.4178204834461212},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3150085210800171},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.20627224445343018},{"id":"https://openalex.org/C70410870","wikidata":"https://www.wikidata.org/wiki/Q199906","display_name":"Clinical psychology","level":1,"score":0.0750795304775238},{"id":"https://openalex.org/C49453240","wikidata":"https://www.wikidata.org/wiki/Q1592163","display_name":"Construct validity","level":3,"score":0.0},{"id":"https://openalex.org/C171606756","wikidata":"https://www.wikidata.org/wiki/Q506132","display_name":"Psychometrics","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000480","descriptor_name":"Alkynes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000693","descriptor_name":"Anaerobiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004229","descriptor_name":"Dithiothreitol","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005182","descriptor_name":"Flavin-Adenine Dinucleotide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005486","descriptor_name":"Flavin Mononucleotide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006885","descriptor_name":"Hydroxybutyrates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013053","descriptor_name":"Spectrophotometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045854","descriptor_name":"Veillonellaceae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1021/bi00588a037","doi":"https://doi.org/10.1021/bi00588a037","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00588a037","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:497163","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/497163","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:kops.uni-konstanz.de:123456789/7545","doi":null,"is_oa":true,"landing_page_url":"http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-57487","pdf_url":"https://kops.uni-konstanz.de/server/api/core/bitstreams/74fa994a-6c50-4700-873a-375bd973bfd8/content","source":{"id":"https://openalex.org/S4306401487","display_name":"KOPS (University of Konstanz)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I189712700","host_organization_name":"University of Konstanz","host_organization_lineage":["https://openalex.org/I189712700"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biochemistry. 1979, 18(21), S. 4724-4732. ISSN 0006-2960. eISSN 1520-4995. Verf\u00fcgbar unter: doi: 10.1021/bi00588a037","raw_type":"doc-type:article"}],"best_oa_location":{"id":"pmh:oai:kops.uni-konstanz.de:123456789/7545","doi":null,"is_oa":true,"landing_page_url":"http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-57487","pdf_url":"https://kops.uni-konstanz.de/server/api/core/bitstreams/74fa994a-6c50-4700-873a-375bd973bfd8/content","source":{"id":"https://openalex.org/S4306401487","display_name":"KOPS (University of Konstanz)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I189712700","host_organization_name":"University of Konstanz","host_organization_lineage":["https://openalex.org/I189712700"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biochemistry. 1979, 18(21), S. 4724-4732. ISSN 0006-2960. eISSN 1520-4995. Verf\u00fcgbar unter: doi: 10.1021/bi00588a037","raw_type":"doc-type:article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2000508531.pdf","grobid_xml":"https://content.openalex.org/works/W2000508531.grobid-xml"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W1262878414","https://openalex.org/W1482480636","https://openalex.org/W1497254911","https://openalex.org/W1526638050","https://openalex.org/W1529670189","https://openalex.org/W1606239426","https://openalex.org/W1608702119","https://openalex.org/W1623580133","https://openalex.org/W1692273962","https://openalex.org/W1964016170","https://openalex.org/W1973415326","https://openalex.org/W1979437065","https://openalex.org/W1979873722","https://openalex.org/W1993065512","https://openalex.org/W1997387075","https://openalex.org/W2000254872","https://openalex.org/W2039825515","https://openalex.org/W2043979050","https://openalex.org/W2052282085","https://openalex.org/W2090138701","https://openalex.org/W2092217437","https://openalex.org/W2131342998","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W1964500962","https://openalex.org/W2052282085","https://openalex.org/W1526638050","https://openalex.org/W1979437065","https://openalex.org/W2058040791","https://openalex.org/W2027467824","https://openalex.org/W2131342998","https://openalex.org/W1972413719","https://openalex.org/W2048054979","https://openalex.org/W2000883930"],"abstract_inverted_index":{"The":[0,77,172],"acetylenic":[1],"alpha-hydroxy":[2,191],"acid":[3,104,163,184,192],"2-hydroxy-3-butynoate":[4],"(alpha":[5],"HB)":[6],"is":[7,45,79,193,202,217],"a":[8,54,92,151],"substrate":[9,174],"and":[10,64,67,81,88,160],"an":[11,71,112,133,155,205],"irreversible":[12],"inactivator":[13],"of":[14,50,142,189,211,214],"the":[15,24,26,48,51,86,101,120,139,182,187,212,218,225],"FAD-containing":[16],"flavoenzyme":[17],"D-lactate":[18],"dehydrogenase":[19],"from":[20,85,186],"Megasphaera":[21],"elsdenii.":[22],"On":[23],"average,":[25],"enzyme":[27,87],"undergoes":[28],"five":[29],"catalytic":[30],"turnovers":[31],"with":[32,57,70,115],"alpha":[33,134,143,215],"HB":[34,135,216],"in":[35,149],"air":[36],"at":[37,61,74],"pH":[38],"7.0":[39],"before":[40],"being":[41],"inactivated.":[42],"Irreversible":[43],"inactivation":[44,129],"due":[46],"to":[47,53,95,100,111,119,126,195,224],"conversion":[49],"flavin":[52,179],"pink":[55],"adduct":[56,78],"visible":[58],"absorption":[59],"peaks":[60],"522,":[62],"382,":[63],"330":[65],"nm":[66],"weak":[68],"fluorescence":[69],"emission":[72],"maximum":[73],"635":[75],"nm.":[76],"stable":[80],"can":[82,107],"be":[83,108,196],"released":[84],"purified.":[89],"It":[90,106],"retains":[91],"structure":[93],"analogous":[94],"FAD":[96],"since":[97],"it":[98,201],"binds":[99,118],"FAD-specific":[102],"apo-D-amino":[103],"oxidase.":[105],"further":[109],"converted":[110],"FMN":[113],"analogue":[114],"phosphodiesterase":[116],"which":[117,221],"FMN-specific":[121],"apoflavodoxin.":[122],"Experiments":[123],"were":[124],"conducted":[125],"test":[127],"whether":[128],"was":[130],"initiated":[131],"by":[132,209],"allene":[136,206],"carbanion":[137,158,207],"or":[138],"dehydrogenation":[140],"product":[141],"HB.":[144],"Kinetic":[145],"studies":[146],"proved":[147],"inconclusive":[148],"that":[150,204],"rapid":[152],"equilibrium":[153],"between":[154],"oxidized":[156],"enzyme--allene":[157],"pair":[159,164],"reduced":[161],"enzyme--keto":[162],"would":[165],"make":[166],"these":[167],"two":[168],"species":[169,220],"kinetically":[170],"equivalent.":[171],"olefinic":[173],"2-hydroxy-3-butenoate,":[175],"however,":[176],"produced":[177,208],"no":[178],"adduct.":[180],"Since":[181],"keto":[183],"derived":[185],"oxidation":[188],"this":[190],"expected":[194],"as":[197,199],"reactive":[198,219],"2-keto-3-butynoate,":[200],"concluded":[203],"abstraction":[210],"alpha-hydrogen":[213],"covalently":[222],"adds":[223],"flavin.":[226]},"counts_by_year":[{"year":2022,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
