{"id":"https://openalex.org/W2000504830","doi":"https://doi.org/10.1016/j.pep.2011.08.018","title":"WITHDRAWN: Reprint of: A new tagged-TEV protease: Construction, optimisation of production, purification and test activity","display_name":"WITHDRAWN: Reprint of: A new tagged-TEV protease: Construction, optimisation of production, purification and test activity","publication_year":2011,"publication_date":"2011-09-01","ids":{"openalex":"https://openalex.org/W2000504830","doi":"https://doi.org/10.1016/j.pep.2011.08.018","mag":"2000504830","pmid":"https://pubmed.ncbi.nlm.nih.gov/21889987"},"language":"en","primary_location":{"id":"doi:10.1016/j.pep.2011.08.018","doi":"https://doi.org/10.1016/j.pep.2011.08.018","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.pep.2011.08.018","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027773111","display_name":"Baligh Miladi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199388852","display_name":"\u00c9cole de Biologie Industrielle","ror":"https://ror.org/029rzwj76","country_code":"FR","type":"education","lineage":["https://openalex.org/I199388852"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Baligh Miladi","raw_affiliation_strings":["Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France","institution_ids":["https://openalex.org/I199388852"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001222058","display_name":"Hassib Bouallagui","orcid":"https://orcid.org/0000-0003-0994-9696","observed_orcids":["https://orcid.org/0000-0003-0994-9696"]},"institutions":[{"id":"https://openalex.org/I42731748","display_name":"National Institute of Applied Science and Technology","ror":"https://ror.org/00qn2rd91","country_code":"TN","type":"facility","lineage":["https://openalex.org/I42731748"]}],"countries":["TN"],"is_corresponding":false,"raw_author_name":"Hassib Bouallagui","raw_affiliation_strings":["Laboratory of Microbial Ecology and Technology, National Institute of Applied Science and Technology, BP 676, Tunis 1080, Tunisia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Microbial Ecology and Technology, National Institute of Applied Science and Technology, BP 676, Tunis 1080, Tunisia","institution_ids":["https://openalex.org/I42731748"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050410226","display_name":"Cyrine Dridi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199388852","display_name":"\u00c9cole de Biologie Industrielle","ror":"https://ror.org/029rzwj76","country_code":"FR","type":"education","lineage":["https://openalex.org/I199388852"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Cyrine Dridi","raw_affiliation_strings":["Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France","institution_ids":["https://openalex.org/I199388852"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028220087","display_name":"Ahmed El Marjou","orcid":"https://orcid.org/0000-0002-0149-428X","observed_orcids":["https://orcid.org/0000-0002-0149-428X"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210161403","display_name":"Biologie cellulaire et Cancer","ror":"https://ror.org/04w11tv37","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I2746051580","https://openalex.org/I39804081","https://openalex.org/I4210096427","https://openalex.org/I4210161403","https://openalex.org/I80043"]},{"id":"https://openalex.org/I80043","display_name":"Institut Curie","ror":"https://ror.org/04t0gwh46","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I80043"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Ahmed El Marjou","raw_affiliation_strings":["Laboratory of Recombinant Proteins, Institute of Curie/CNRS UMR144, Paris, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Recombinant Proteins, Institute of Curie/CNRS UMR144, Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210161403","https://openalex.org/I80043"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020177855","display_name":"Guilhem Boeuf","orcid":"https://orcid.org/0000-0003-2680-6750","observed_orcids":["https://orcid.org/0000-0003-2680-6750"]},"institutions":[{"id":"https://openalex.org/I199388852","display_name":"\u00c9cole de Biologie Industrielle","ror":"https://ror.org/029rzwj76","country_code":"FR","type":"education","lineage":["https://openalex.org/I199388852"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Guilhem Boeuf","raw_affiliation_strings":["Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France","institution_ids":["https://openalex.org/I199388852"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073581274","display_name":"Patrick Di Martino","orcid":"https://orcid.org/0000-0001-5450-2733","observed_orcids":["https://orcid.org/0000-0001-5450-2733"]},"institutions":[{"id":"https://openalex.org/I4387155829","display_name":"Universit\u00e9 de Cergy-Pontoise","ror":"https://ror.org/03vs46t66","country_code":"FR","type":"education","lineage":["https://openalex.org/I4210142324","https://openalex.org/I4387155829"]},{"id":"https://openalex.org/I4405252942","display_name":"\u00c9quipe de Recherche sur les Relations Matrice Extracellulaire-Cellules","ror":"https://ror.org/004yvak38","country_code":"FR","type":"facility","lineage":["https://openalex.org/I4210142324","https://openalex.org/I4405252942","https://openalex.org/I4405256634"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Patrick Di Martino","raw_affiliation_strings":["ERRMECe Laboratory (EA1391), University of Cergy-Pontoise, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ERRMECe Laboratory (EA1391), University of Cergy-Pontoise, France","institution_ids":["https://openalex.org/I4387155829","https://openalex.org/I4405252942"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017010632","display_name":"Florence Dufour","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199388852","display_name":"\u00c9cole de Biologie Industrielle","ror":"https://ror.org/029rzwj76","country_code":"FR","type":"education","lineage":["https://openalex.org/I199388852"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Florence Dufour","raw_affiliation_strings":["Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France","institution_ids":["https://openalex.org/I199388852"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109509805","display_name":"Abdellatif Elm\u2019selmi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199388852","display_name":"\u00c9cole de Biologie Industrielle","ror":"https://ror.org/029rzwj76","country_code":"FR","type":"education","lineage":["https://openalex.org/I199388852"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Abdellatif Elm\u2019Selmi","raw_affiliation_strings":["Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Biology, Ecole de Biologie Industrielle, 32 Bd du Port, Cergy 95094, France","institution_ids":["https://openalex.org/I199388852"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5109509805"],"corresponding_institution_ids":["https://openalex.org/I199388852"],"apc_list":{"value":3680,"currency":"USD","value_usd":3680},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.08531057,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"","last_page":""},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9585000276565552,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9585000276565552,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.01590000092983246,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.014499999582767487,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tobacco-etch-virus-protease","display_name":"tobacco etch virus protease","score":0.8920000195503235},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7979999780654907},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7910000085830688},{"id":"https://openalex.org/keywords/induction-temperature","display_name":"induction temperature","score":0.7390000224113464},{"id":"https://openalex.org/keywords/protein-solubility","display_name":"protein solubility","score":0.8769999742507935},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.7710000276565552}],"concepts":[{"id":"https://openalex.org/C2778489119","wikidata":"https://www.wikidata.org/wiki/Q1962297","display_name":"Reprint","level":2,"score":0.8369758129119873},{"id":"https://openalex.org/C126965237","wikidata":"https://www.wikidata.org/wiki/Q816335","display_name":"Accidental","level":2,"score":0.5416342616081238},{"id":"https://openalex.org/C2778348673","wikidata":"https://www.wikidata.org/wiki/Q739302","display_name":"Production (economics)","level":2,"score":0.528967559337616},{"id":"https://openalex.org/C2777267654","wikidata":"https://www.wikidata.org/wiki/Q3519023","display_name":"Test (biology)","level":2,"score":0.47346821427345276},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.340214341878891},{"id":"https://openalex.org/C42475967","wikidata":"https://www.wikidata.org/wiki/Q194292","display_name":"Operations research","level":1,"score":0.3372979760169983},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.29087013006210327},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.27842846512794495},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.16599395871162415},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.10248702764511108},{"id":"https://openalex.org/C1276947","wikidata":"https://www.wikidata.org/wiki/Q333","display_name":"Astronomy","level":1,"score":0.0},{"id":"https://openalex.org/C139719470","wikidata":"https://www.wikidata.org/wiki/Q39680","display_name":"Macroeconomics","level":1,"score":0.0},{"id":"https://openalex.org/C24890656","wikidata":"https://www.wikidata.org/wiki/Q82811","display_name":"Acoustics","level":1,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1016/j.pep.2011.08.018","doi":"https://doi.org/10.1016/j.pep.2011.08.018","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.pep.2011.08.018","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},{"id":"pmid:21889987","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21889987","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein expression and purification","raw_type":"Journal Article"},{"id":"pmh:oai:HAL:hal-01951087v1","doi":null,"is_oa":false,"landing_page_url":"https://hal.science/hal-01951087","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Protein Expression and Purification, 2011","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2050966688","https://openalex.org/W2087098118","https://openalex.org/W3211990777","https://openalex.org/W2001777806","https://openalex.org/W7099968006","https://openalex.org/W196616756","https://openalex.org/W4210354537","https://openalex.org/W1967205134","https://openalex.org/W2286188951","https://openalex.org/W1984040042"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-10-25T09:29:13.331239","created_date":"2016-06-24T00:00:00"}