{"id":"https://openalex.org/W2000454848","doi":"https://doi.org/10.1074/jbc.m506941200","title":"Several Acidic Amino Acids in the N-domain of Insulin-like Growth Factor-binding Protein-5 Are Important for Its Transactivation Activity","display_name":"Several Acidic Amino Acids in the N-domain of Insulin-like Growth Factor-binding Protein-5 Are Important for Its Transactivation Activity","publication_year":2006,"publication_date":"2006-03-17","ids":{"openalex":"https://openalex.org/W2000454848","doi":"https://doi.org/10.1074/jbc.m506941200","mag":"2000454848","pmid":"https://pubmed.ncbi.nlm.nih.gov/16543235"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m506941200","doi":"https://doi.org/10.1074/jbc.m506941200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m506941200","pdf_url":"http://www.jbc.org/article/S0021925820780622/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925820780622/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101639767","display_name":"Yang O. Zhao","orcid":"https://orcid.org/0009-0006-7781-3941","observed_orcids":["https://orcid.org/0009-0006-7781-3941"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yang Zhao","raw_affiliation_strings":["Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057077440","display_name":"Ping Yin","orcid":"https://orcid.org/0000-0001-8001-221X","observed_orcids":["https://orcid.org/0000-0001-8001-221X","https://orcid.org/0009-0009-6369-2140"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ping Yin","raw_affiliation_strings":["Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086610862","display_name":"Leon A. Bach","orcid":"https://orcid.org/0000-0002-9062-1518","observed_orcids":["https://orcid.org/0000-0002-9062-1518"]},"institutions":[{"id":"https://openalex.org/I2801993434","display_name":"The Alfred Hospital","ror":"https://ror.org/01wddqe20","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2801993434","https://openalex.org/I4210163178"]},{"id":"https://openalex.org/I56590836","display_name":"Monash University","ror":"https://ror.org/02bfwt286","country_code":"AU","type":"education","lineage":["https://openalex.org/I56590836"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Leon A. Bach","raw_affiliation_strings":["Department of Medicine and Department of Endocrinology and Diabetes, Monash University, Alfred Hospital, Melbourne, Victoria 3004, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine and Department of Endocrinology and Diabetes, Monash University, Alfred Hospital, Melbourne, Victoria 3004, Australia","institution_ids":["https://openalex.org/I2801993434","https://openalex.org/I56590836"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086749248","display_name":"Cunming Duan","orcid":"https://orcid.org/0000-0001-6794-2762","observed_orcids":["https://orcid.org/0000-0001-6794-2762"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Cunming Duan","raw_affiliation_strings":["Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5086749248"],"corresponding_institution_ids":["https://openalex.org/I27837315"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.7173,"has_fulltext":true,"cited_by_count":52,"citation_normalized_percentile":{"value":0.89908222,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"281","issue":"20","first_page":"14184","last_page":"14191"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/igfbp-5","display_name":"IGFBP-5","score":0.9649999737739563},{"id":"https://openalex.org/keywords/transactivation-activity","display_name":"transactivation activity","score":0.7329999804496765},{"id":"https://openalex.org/keywords/n-terminal-domain","display_name":"N-terminal domain","score":0.765999972820282},{"id":"https://openalex.org/keywords/acidic-amino-acids","display_name":"acidic amino acids","score":0.9620000123977661},{"id":"https://openalex.org/keywords/nuclear-localization","display_name":"nuclear localization","score":0.7229999899864197},{"id":"https://openalex.org/keywords/histone-dna-complex","display_name":"histone-DNA complex","score":0.8669999837875366},{"id":"https://openalex.org/keywords/negative-regulatory-element","display_name":"negative regulatory element","score":0.7329999804496765},{"id":"https://openalex.org/keywords/igfbp-2","display_name":"IGFBP-2","score":0.8339999914169312},{"id":"https://openalex.org/keywords/igfbp-3","display_name":"IGFBP-3","score":0.9990000128746033},{"id":"https://openalex.org/keywords/conserved-residues","display_name":"conserved residues","score":0.6930000185966492},{"id":"https://openalex.org/keywords/transcriptional-regulation","display_name":"transcriptional regulation","score":0.5339999794960022}],"concepts":[{"id":"https://openalex.org/C1292079","wikidata":"https://www.wikidata.org/wiki/Q3817769","display_name":"Transactivation","level":4,"score":0.9857286214828491},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6605424284934998},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5336208343505859},{"id":"https://openalex.org/C134320426","wikidata":"https://www.wikidata.org/wiki/Q901026","display_name":"Chromatin immunoprecipitation","level":5,"score":0.5110765695571899},{"id":"https://openalex.org/C71829478","wikidata":"https://www.wikidata.org/wiki/Q901305","display_name":"Immunoprecipitation","level":3,"score":0.47848695516586304},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.44603046774864197},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4356098175048828},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3911474645137787},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.3779582977294922},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3517206311225891},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13772791624069214},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.0976824164390564},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006657","descriptor_name":"Histones","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015533","descriptor_name":"Transcriptional Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018971","descriptor_name":"Insulin-Like Growth Factor Binding Protein 2","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018972","descriptor_name":"Insulin-Like Growth Factor Binding Protein 3","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018975","descriptor_name":"Insulin-Like Growth Factor Binding Protein 5","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018975","descriptor_name":"Insulin-Like Growth Factor Binding Protein 5","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m506941200","doi":"https://doi.org/10.1074/jbc.m506941200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m506941200","pdf_url":"http://www.jbc.org/article/S0021925820780622/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:16543235","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16543235","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m506941200","doi":"https://doi.org/10.1074/jbc.m506941200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m506941200","pdf_url":"http://www.jbc.org/article/S0021925820780622/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5492296399","display_name":"IGF-I and its binding proteins in vascular smooth muscle cells","funder_award_id":"R01HL060679","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G5872199422","display_name":"Ligand-dependent and -independent actions of insulin-like growth factor binding proteins in fish","funder_award_id":"0543018","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332600","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2000454848.pdf","grobid_xml":"https://content.openalex.org/works/W2000454848.grobid-xml"},"referenced_works_count":49,"referenced_works":["https://openalex.org/W1569741533","https://openalex.org/W1584406385","https://openalex.org/W1936612717","https://openalex.org/W1966516812","https://openalex.org/W1972273837","https://openalex.org/W1975213791","https://openalex.org/W1976299678","https://openalex.org/W1977236952","https://openalex.org/W1985987710","https://openalex.org/W1991095866","https://openalex.org/W1993635973","https://openalex.org/W2000394884","https://openalex.org/W2001641438","https://openalex.org/W2006345660","https://openalex.org/W2012505775","https://openalex.org/W2020308645","https://openalex.org/W2023211332","https://openalex.org/W2024642928","https://openalex.org/W2024707576","https://openalex.org/W2027454234","https://openalex.org/W2028246431","https://openalex.org/W2029098538","https://openalex.org/W2034967926","https://openalex.org/W2038163496","https://openalex.org/W2039756216","https://openalex.org/W2041327254","https://openalex.org/W2041807810","https://openalex.org/W2045015715","https://openalex.org/W2053099348","https://openalex.org/W2053296976","https://openalex.org/W2058196624","https://openalex.org/W2059784252","https://openalex.org/W2071482027","https://openalex.org/W2081062649","https://openalex.org/W2083106037","https://openalex.org/W2086867296","https://openalex.org/W2095385109","https://openalex.org/W2105014300","https://openalex.org/W2105803506","https://openalex.org/W2112981515","https://openalex.org/W2120622189","https://openalex.org/W2123782952","https://openalex.org/W2123930465","https://openalex.org/W2132629607","https://openalex.org/W2133386615","https://openalex.org/W2133647910","https://openalex.org/W2136140283","https://openalex.org/W2171971275","https://openalex.org/W2232689465"],"related_works":["https://openalex.org/W3149744123","https://openalex.org/W2029744067","https://openalex.org/W2619393461","https://openalex.org/W2171971275","https://openalex.org/W7100350205","https://openalex.org/W2747707927","https://openalex.org/W2024642928","https://openalex.org/W2034612795","https://openalex.org/W2112981515","https://openalex.org/W1978246731"],"abstract_inverted_index":{"Insulin-like":[0],"growth":[1],"factor-binding":[2],"protein":[3,8],"(IGFBP)-5":[4],"is":[5,18,80],"a":[6,58,172,175],"secreted":[7],"that":[9,49,132,156,209,220,226],"binds":[10],"to":[11,70,148,160,174],"IGFs":[12],"and":[13,27,90,100,111,169,188,225,228],"modulates":[14],"IGF":[15],"actions.":[16],"IGFBP-5":[17,39,50,67,79,130,217],"also":[19,102,230],"found":[20],"in":[21,40,87,128,136,142,215,235],"the":[22,34,53,63,75,88,93,96,129,149,216],"nuclei":[23],"of":[24,37,60,66,78,98,109,140],"cultured":[25],"cells":[26],"has":[28],"transactivation":[29,64,76,105,182,202,224,233],"activity.":[30,115,121,183,195,203],"Here":[31],"we":[32],"report":[33],"nuclear":[35,54],"localization":[36],"endogenous":[38],"mouse":[41],"embryonic":[42],"skeletal":[43],"cells.":[44],"Chromatin":[45],"immunoprecipitation":[46],"experiments":[47],"indicated":[48],"interacts":[51],"with":[52],"histone-DNA":[55],"complex.":[56],"Using":[57],"series":[59],"deletion":[61],"mutants,":[62],"domain":[65],"was":[68],"mapped":[69],"its":[71],"N-terminal":[72,218],"region.":[73],"Intriguingly,":[74],"activity":[77,234],"masked":[81],"by":[82],"negative":[83],"regulatory":[84],"elements":[85],"located":[86],"L-":[89],"C-domains.":[91],"Among":[92],"other":[94],"IGFBPs,":[95],"N-domains":[97],"IGFBP-2":[99,227],"-3":[101,229],"had":[103,113,119,199],"strong":[104,232],"activity,":[106],"whereas":[107],"those":[108],"IGFBP-1":[110,151],"-6":[112],"no":[114,201],"The":[116,138,184,196],"IGFBP-4":[117],"N-domain":[118,131],"modest":[120],"Sequence":[122],"analysis":[123],"revealed":[124],"several":[125,212],"amino":[126],"acids":[127],"are":[133,211,221],"not":[134],"present":[135],"IGFBP-1.":[137],"activities":[139],"mutants":[141,191,198],"which":[143],"these":[144],"residues":[145,159,162,214],"were":[146,153],"changed":[147,157],"corresponding":[150],"sequence":[152],"determined.":[154],"Mutations":[155],"acidic":[158],"neutral":[161],"(e.g.":[163,178],"E8A,":[164],"D11S,":[165],"E12A,":[166],"E30S/P31A,":[167],"E43L,":[168],"E52A)":[170],"or":[171],"polar":[173],"basic":[176],"residue":[177],"Q56R)":[179],"significantly":[180],"reduced":[181,194],"E8A/D11S/E12A":[185],"triple":[186],"mutant":[187],"E52A/Q56R":[189],"double":[190],"showed":[192],"further":[193],"combinatory":[197],"essentially":[200],"Taken":[204],"together,":[205],"our":[206],"results":[207],"indicate":[208],"there":[210],"conserved":[213],"region":[219],"critical":[222],"for":[223],"have":[231],"their":[236],"N-domains.":[237]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":9},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}