{"id":"https://openalex.org/W2000373128","doi":"https://doi.org/10.1177/24.3.57192","title":"Ultrastructural localization of viral antigens using the unlabeled antibody-enzyme method.","display_name":"Ultrastructural localization of viral antigens using the unlabeled antibody-enzyme method.","publication_year":1976,"publication_date":"1976-03-01","ids":{"openalex":"https://openalex.org/W2000373128","doi":"https://doi.org/10.1177/24.3.57192","mag":"2000373128","pmid":"https://pubmed.ncbi.nlm.nih.gov/57192"},"language":"en","primary_location":{"id":"doi:10.1177/24.3.57192","doi":"https://doi.org/10.1177/24.3.57192","is_oa":false,"landing_page_url":"https://doi.org/10.1177/24.3.57192","pdf_url":null,"source":{"id":"https://openalex.org/S30841621","display_name":"Journal of Histochemistry & Cytochemistry","issn_l":"0022-1554","issn":["0022-1554","1551-5044"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Histochemistry & Cytochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5048626472","display_name":"Gwen Wendelschafer\u2010Crabb","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G Wendelschafer-Crabb","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110121109","display_name":"STANLEY L. ERLANDSEN","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S L Erlandsen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5035223547","display_name":"Donald H. Walker","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D H Walker","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4400,"currency":"USD","value_usd":4400},"apc_paid":null,"fwci":1.0298,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.71275456,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"24","issue":"3","first_page":"517","last_page":"526"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.006599999964237213,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14134","display_name":"Classical Anatomy and Histology","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/2702","display_name":"Anatomy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/viral-antigen-localization","display_name":"viral antigen localization","score":0.8069999814033508},{"id":"https://openalex.org/keywords/bacteriophage-p1","display_name":"bacteriophage P1","score":0.9660000205039978},{"id":"https://openalex.org/keywords/shigella-dysenteriae","display_name":"Shigella dysenteriae","score":0.996999979019165}],"concepts":[{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.8544018268585205},{"id":"https://openalex.org/C4224716","wikidata":"https://www.wikidata.org/wiki/Q10859258","display_name":"Immunostaining","level":3,"score":0.6809378862380981},{"id":"https://openalex.org/C2780610775","wikidata":"https://www.wikidata.org/wiki/Q265094","display_name":"Shigella dysenteriae","level":4,"score":0.5466757416725159},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.522036075592041},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.486066609621048},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.4848105013370514},{"id":"https://openalex.org/C87555872","wikidata":"https://www.wikidata.org/wiki/Q3141316","display_name":"Ultrastructure","level":2,"score":0.48315876722335815},{"id":"https://openalex.org/C166029249","wikidata":"https://www.wikidata.org/wiki/Q3772564","display_name":"Negative stain","level":3,"score":0.4413837790489197},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3650399148464203},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3564700782299042},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.33149266242980957},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.3190731406211853},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.27943891286849976},{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.22923767566680908},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14422297477722168},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1408122181892395},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1290687620639801},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.11693504452705383},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.08601304888725281},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000937","descriptor_name":"Antigen-Antibody Reactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000956","descriptor_name":"Antigens, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D001435","descriptor_name":"Bacteriophages","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001435","descriptor_name":"Bacteriophages","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D005455","descriptor_name":"Fluorescent Antibody Technique","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D012760","descriptor_name":"Shigella","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D012760","descriptor_name":"Shigella","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1177/24.3.57192","doi":"https://doi.org/10.1177/24.3.57192","is_oa":false,"landing_page_url":"https://doi.org/10.1177/24.3.57192","pdf_url":null,"source":{"id":"https://openalex.org/S30841621","display_name":"Journal of Histochemistry & Cytochemistry","issn_l":"0022-1554","issn":["0022-1554","1551-5044"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Histochemistry & Cytochemistry","raw_type":"journal-article"},{"id":"pmid:57192","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/57192","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1021421451","https://openalex.org/W2051539744","https://openalex.org/W2363881654","https://openalex.org/W2037861084","https://openalex.org/W67951489","https://openalex.org/W3036816562","https://openalex.org/W2000039998","https://openalex.org/W4405328789","https://openalex.org/W1989370774","https://openalex.org/W1969129898"],"abstract_inverted_index":{"Employing":[0],"the":[1,7,21,31,46,58,63,68,73,84,95,109,124,129,137],"unlabeled":[2],"antibody":[3],"enzyme":[4],"method":[5,66,104],"at":[6],"ultrastructural":[8],"level,":[9],"a":[10,39,78],"comparison":[11],"was":[12,36,134,146],"made":[13],"between":[14],"preembedding":[15,64,110],"staining":[16,19,43,65,82,103,111],"and":[17,83,139],"postembedding":[18,102],"for":[20],"detection":[22],"of":[23,33,49,62,70,80,88,97,132,143],"viral":[24,59,121],"antigens.":[25],"The":[26,101],"bacteriophage":[27],"P1":[28],"absorbed":[29],"to":[30],"surface":[32],"Shigella":[34],"dysenteriae":[35],"used":[37],"as":[38,53],"model":[40],"system.":[41],"Preembedding":[42],"resulted":[44],"in":[45,112],"specific":[47,81,130],"deposition":[48],"peroxidase-antiperoxidase":[50],"(PAP)":[51],"complexes":[52,115],"an":[54],"electron-dense":[55],"coating":[56],"around":[57],"heads.":[60],"Disadvantages":[61],"included":[67],"agglutination":[69],"cells":[71],"by":[72],"primary":[74],"antiserum":[75],"which":[76],"produced":[77],"gradient":[79],"\"bleeding\"":[85],"or":[86],"migration":[87,142],"electron":[89],"dense":[90],"reaction":[91,144],"product":[92,145],"away":[93],"from":[94],"sites":[96],"attached":[98],"PAP":[99,114],"complexes.":[100],"had":[105],"distinct":[106],"advantages":[107],"over":[108,119],"that":[113],"were":[116],"deposited":[117],"directly":[118],"exposed":[120],"heads":[122],"within":[123],"thin":[125],"section.":[126],"In":[127],"addition,":[128],"immunostaining":[131],"viruses":[133],"uniform":[135],"through":[136],"section":[138],"no":[140],"artifactual":[141],"observed.":[147]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}