{"id":"https://openalex.org/W2000320799","doi":"https://doi.org/10.1016/0021-9150(96)05816-9","title":"Adenovirus-assisted lipofection: efficient in vitro gene transfer of luciferase and cytosine deaminase to human smooth muscle cells","display_name":"Adenovirus-assisted lipofection: efficient in vitro gene transfer of luciferase and cytosine deaminase to human smooth muscle cells","publication_year":1996,"publication_date":"1996-07-01","ids":{"openalex":"https://openalex.org/W2000320799","doi":"https://doi.org/10.1016/0021-9150(96)05816-9","mag":"2000320799","pmid":"https://pubmed.ncbi.nlm.nih.gov/8800493"},"language":"en","primary_location":{"id":"doi:10.1016/0021-9150(96)05816-9","doi":"https://doi.org/10.1016/0021-9150(96)05816-9","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0021-9150(96)05816-9","pdf_url":null,"source":{"id":"https://openalex.org/S206830620","display_name":"Atherosclerosis","issn_l":"0021-9150","issn":["0021-9150","1879-1484"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Atherosclerosis","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111493214","display_name":"J. Kreuzer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"J. Kreuzer","raw_affiliation_strings":["Innere Medizin III, Universit\u00e4t Heidelberg, Germany. JOERGKREUZER@KRZMAIL.KRZ.UNI-HEIDELBERG.DE","Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Innere Medizin III, Universit\u00e4t Heidelberg, Germany. JOERGKREUZER@KRZMAIL.KRZ.UNI-HEIDELBERG.DE","institution_ids":["https://openalex.org/I223822909"]},{"raw_affiliation_string":"Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111746389","display_name":"S. Denger","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"S. Denger","raw_affiliation_strings":["Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072648742","display_name":"Frank Reifers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"F. Reifers","raw_affiliation_strings":["Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053668445","display_name":"Christian Beisel","orcid":"https://orcid.org/0000-0001-5360-2193","observed_orcids":["https://orcid.org/0000-0001-5360-2193","https://orcid.org/0000-0001-6661-6957"]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"C. Beisel","raw_affiliation_strings":["Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083104890","display_name":"Karin Haack","orcid":"https://orcid.org/0000-0003-3067-3575","observed_orcids":["https://orcid.org/0000-0003-3067-3575"]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]},{"id":"https://openalex.org/I4210165970","display_name":"Chirurgische Universit\u00e4tsklinik Heidelberg","ror":"https://ror.org/05fe3fx56","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I2802164966","https://openalex.org/I4210165970"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"K. Haack","raw_affiliation_strings":["Sektion Molekulare Diagnostik und Therapie, Chirurgische Universit\u00e4tsklinik, Universit\u00e4t Heidelberg, Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sektion Molekulare Diagnostik und Therapie, Chirurgische Universit\u00e4tsklinik, Universit\u00e4t Heidelberg, Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909","https://openalex.org/I4210165970"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021806130","display_name":"Johannes F. Gebert","orcid":"https://orcid.org/0000-0002-0606-7818","observed_orcids":["https://orcid.org/0000-0002-0606-7818"]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]},{"id":"https://openalex.org/I4210165970","display_name":"Chirurgische Universit\u00e4tsklinik Heidelberg","ror":"https://ror.org/05fe3fx56","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I2802164966","https://openalex.org/I4210165970"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"J. Gebert","raw_affiliation_strings":["Sektion Molekulare Diagnostik und Therapie, Chirurgische Universit\u00e4tsklinik, Universit\u00e4t Heidelberg, Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sektion Molekulare Diagnostik und Therapie, Chirurgische Universit\u00e4tsklinik, Universit\u00e4t Heidelberg, Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909","https://openalex.org/I4210165970"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111762241","display_name":"W. K\u00fcbler","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"W. K\u00fcbler","raw_affiliation_strings":["Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Innere Medizin III, Universit\u00e4t Heidelberg, Bergheimer Str. 58, 69115 Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111493214"],"corresponding_institution_ids":["https://openalex.org/I223822909"],"apc_list":{"value":4020,"currency":"USD","value_usd":4020},"apc_paid":null,"fwci":1.4899,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.80207956,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"124","issue":"1","first_page":"49","last_page":"60"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9018999934196472,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9018999934196472,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.09480000287294388,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/smooth-muscle-cells","display_name":"smooth muscle cells","score":0.7689999938011169},{"id":"https://openalex.org/keywords/gene-transfer","display_name":"gene transfer","score":0.8410000205039978},{"id":"https://openalex.org/keywords/cytosine-deaminase","display_name":"cytosine deaminase","score":0.8399999737739563},{"id":"https://openalex.org/keywords/firefly-luciferase","display_name":"firefly luciferase","score":0.7710000276565552},{"id":"https://openalex.org/keywords/5-fluorocytosine","display_name":"5-fluorocytosine","score":0.8429999947547913},{"id":"https://openalex.org/keywords/5-fluorouracil","display_name":"5-fluorouracil","score":0.9490000009536743},{"id":"https://openalex.org/keywords/smooth-muscle-cell-proliferation","display_name":"smooth muscle cell proliferation","score":0.699999988079071},{"id":"https://openalex.org/keywords/restenosis","display_name":"restenosis","score":0.7390000224113464},{"id":"https://openalex.org/keywords/coronary-artery-disease","display_name":"coronary artery disease","score":0.8009999990463257},{"id":"https://openalex.org/keywords/replication-deficient-adenovirus","display_name":"replication-defective adenovirus","score":0.8220000267028809},{"id":"https://openalex.org/keywords/vascular-gene-therapy","display_name":"vascular gene therapy","score":0.5720000267028809}],"concepts":[{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.8114781379699707},{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.7177583575248718},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6885405778884888},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.6324962973594666},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.625529944896698},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.5867996215820312},{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.5617769360542297},{"id":"https://openalex.org/C2777748644","wikidata":"https://www.wikidata.org/wiki/Q5201383","display_name":"Cytosine deaminase","level":4,"score":0.538374125957489},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.49375924468040466},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.48862892389297485},{"id":"https://openalex.org/C144251240","wikidata":"https://www.wikidata.org/wiki/Q7068229","display_name":"Nucleofection","level":4,"score":0.4777909219264984},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4076007306575775},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3316161036491394},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.328064888715744},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3068176507949829},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.09040337800979614},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000260","descriptor_name":"Adenoviruses, Human","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D001011","descriptor_name":"Aorta","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002130","descriptor_name":"Calcium Phosphates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002411","descriptor_name":"Cation Exchange Resins","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003673","descriptor_name":"Defective Viruses","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008055","descriptor_name":"Lipids","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":true},{"descriptor_ui":"D008081","descriptor_name":"Liposomes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009131","descriptor_name":"Muscle, Smooth, Vascular","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D009131","descriptor_name":"Muscle, Smooth, Vascular","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009700","descriptor_name":"Nucleoside Deaminases","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D009700","descriptor_name":"Nucleoside Deaminases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D043525","descriptor_name":"Cytosine Deaminase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0021-9150(96)05816-9","doi":"https://doi.org/10.1016/0021-9150(96)05816-9","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0021-9150(96)05816-9","pdf_url":null,"source":{"id":"https://openalex.org/S206830620","display_name":"Atherosclerosis","issn_l":"0021-9150","issn":["0021-9150","1879-1484"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Atherosclerosis","raw_type":"journal-article"},{"id":"pmid:8800493","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8800493","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Atherosclerosis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9476000070571899,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310693","display_name":"Deutsches Krebsforschungszentrum","ror":"https://ror.org/04cdgtt98"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":41,"referenced_works":["https://openalex.org/W140890592","https://openalex.org/W199840660","https://openalex.org/W976079700","https://openalex.org/W1525669528","https://openalex.org/W1590372415","https://openalex.org/W1606477282","https://openalex.org/W1775749144","https://openalex.org/W1965319333","https://openalex.org/W1966439732","https://openalex.org/W1968082320","https://openalex.org/W1971290197","https://openalex.org/W1974926996","https://openalex.org/W1976579475","https://openalex.org/W2005920135","https://openalex.org/W2008366682","https://openalex.org/W2008870095","https://openalex.org/W2017593380","https://openalex.org/W2018216570","https://openalex.org/W2025563293","https://openalex.org/W2030823294","https://openalex.org/W2036650398","https://openalex.org/W2044296969","https://openalex.org/W2048253459","https://openalex.org/W2063250637","https://openalex.org/W2079332181","https://openalex.org/W2081580391","https://openalex.org/W2090343238","https://openalex.org/W2092950196","https://openalex.org/W2097794242","https://openalex.org/W2100837269","https://openalex.org/W2118479681","https://openalex.org/W2131461932","https://openalex.org/W2137570167","https://openalex.org/W2144634347","https://openalex.org/W2147643233","https://openalex.org/W2411047874","https://openalex.org/W2417711872","https://openalex.org/W2474180753","https://openalex.org/W3147410449","https://openalex.org/W6654587008","https://openalex.org/W6673685826"],"related_works":["https://openalex.org/W2063250637","https://openalex.org/W2409195978","https://openalex.org/W2066478217","https://openalex.org/W2159110010","https://openalex.org/W3190534692","https://openalex.org/W2081738353","https://openalex.org/W2076829876","https://openalex.org/W2035128455","https://openalex.org/W2921931997","https://openalex.org/W2021503026"],"abstract_inverted_index":null,"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}