{"id":"https://openalex.org/W2000109035","doi":"https://doi.org/10.2202/1556-3758.1464","title":"Study on Immobilization Methods of Acetylcholinesterase","display_name":"Study on Immobilization Methods of Acetylcholinesterase","publication_year":2008,"publication_date":"2008-11-17","ids":{"openalex":"https://openalex.org/W2000109035","doi":"https://doi.org/10.2202/1556-3758.1464","mag":"2000109035"},"language":"en","primary_location":{"id":"doi:10.2202/1556-3758.1464","doi":"https://doi.org/10.2202/1556-3758.1464","is_oa":false,"landing_page_url":"https://doi.org/10.2202/1556-3758.1464","pdf_url":null,"source":{"id":"https://openalex.org/S1488397","display_name":"International Journal of Food Engineering","issn_l":"1556-3758","issn":["1556-3758","2194-5764"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310313990","host_organization_name":"De Gruyter","host_organization_lineage":["https://openalex.org/P4310313990"],"host_organization_lineage_names":["De Gruyter"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Food Engineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058875876","display_name":"Xia Sun","orcid":"https://orcid.org/0000-0001-6074-0615","observed_orcids":["https://orcid.org/0000-0001-6074-0615","https://orcid.org/0000-0002-1006-4505","https://orcid.org/0009-0002-5222-3575"]},"institutions":[{"id":"https://openalex.org/I52158045","display_name":"China Agricultural University","ror":"https://ror.org/04v3ywz14","country_code":"CN","type":"education","lineage":["https://openalex.org/I52158045"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xia Sun","raw_affiliation_strings":["China Agricultural University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"China Agricultural University","institution_ids":["https://openalex.org/I52158045"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026077679","display_name":"Xiangyou Wang","orcid":"https://orcid.org/0000-0002-0422-2642","observed_orcids":["https://orcid.org/0000-0002-0422-2642"]},"institutions":[{"id":"https://openalex.org/I119203015","display_name":"Shandong University of Technology","ror":"https://ror.org/02mr3ar13","country_code":"CN","type":"education","lineage":["https://openalex.org/I119203015"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiangyou Wang","raw_affiliation_strings":["Shandong University of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shandong University of Technology","institution_ids":["https://openalex.org/I119203015"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101465948","display_name":"Zhe Liu","orcid":"https://orcid.org/0000-0003-0799-5525","observed_orcids":["https://orcid.org/0000-0003-0799-5525","https://orcid.org/0000-0003-3076-1057"]},"institutions":[{"id":"https://openalex.org/I56590836","display_name":"Monash University","ror":"https://ror.org/02bfwt286","country_code":"AU","type":"education","lineage":["https://openalex.org/I56590836"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Zhe Liu","raw_affiliation_strings":["Monash University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Monash University","institution_ids":["https://openalex.org/I56590836"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":2400,"currency":"EUR","value_usd":2712},"apc_paid":null,"fwci":1.1492,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.72715573,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"4","issue":"8","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10212","display_name":"Electrochemical sensors and biosensors","score":0.7973999977111816,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10212","display_name":"Electrochemical sensors and biosensors","score":0.7973999977111816,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.19200000166893005,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11434","display_name":"Electrochemical Analysis and Applications","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1603","display_name":"Electrochemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acetylcholinesterase","display_name":"acetylcholinesterase","score":0.9869999885559082},{"id":"https://openalex.org/keywords/enzyme-immobilization","display_name":"enzyme immobilization","score":0.9980000257492065},{"id":"https://openalex.org/keywords/glassy-carbon-electrode","display_name":"glassy carbon electrode","score":0.8600000143051147},{"id":"https://openalex.org/keywords/amperometric-biosensor","display_name":"amperometric biosensor","score":0.8410000205039978},{"id":"https://openalex.org/keywords/organophosphorus-pesticide-detection","display_name":"organophosphorus pesticide detection","score":0.9359999895095825},{"id":"https://openalex.org/keywords/glutaraldehyde-crosslinking","display_name":"glutaraldehyde crosslinking","score":0.9340000152587891},{"id":"https://openalex.org/keywords/chitosan-membranes","display_name":"chitosan membranes","score":0.968999981880188},{"id":"https://openalex.org/keywords/acetylthiocholine-iodide","display_name":"acetylthiocholine iodide","score":0.8009999990463257}],"concepts":[{"id":"https://openalex.org/C2777529624","wikidata":"https://www.wikidata.org/wiki/Q416475","display_name":"Glutaraldehyde","level":2,"score":0.8708099722862244},{"id":"https://openalex.org/C2778816929","wikidata":"https://www.wikidata.org/wiki/Q22676679","display_name":"Acetylcholinesterase","level":3,"score":0.7845979928970337},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.721488356590271},{"id":"https://openalex.org/C160756335","wikidata":"https://www.wikidata.org/wiki/Q669391","display_name":"Biosensor","level":2,"score":0.6882947087287903},{"id":"https://openalex.org/C139369640","wikidata":"https://www.wikidata.org/wiki/Q474536","display_name":"Amperometry","level":4,"score":0.653496265411377},{"id":"https://openalex.org/C33633552","wikidata":"https://www.wikidata.org/wiki/Q904145","display_name":"Immobilized enzyme","level":3,"score":0.5999132394790649},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5667649507522583},{"id":"https://openalex.org/C86807702","wikidata":"https://www.wikidata.org/wiki/Q2620381","display_name":"Bovine serum albumin","level":2,"score":0.5518572330474854},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.48127835988998413},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.47253966331481934},{"id":"https://openalex.org/C2909509320","wikidata":"https://www.wikidata.org/wiki/Q27458037","display_name":"Acetylthiocholine","level":5,"score":0.47217363119125366},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.431831955909729},{"id":"https://openalex.org/C2779059548","wikidata":"https://www.wikidata.org/wiki/Q383701","display_name":"Ach\u00e9","level":4,"score":0.32037991285324097},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.20068520307540894},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1856841742992401},{"id":"https://openalex.org/C17525397","wikidata":"https://www.wikidata.org/wiki/Q176140","display_name":"Electrode","level":2,"score":0.18134188652038574},{"id":"https://openalex.org/C52859227","wikidata":"https://www.wikidata.org/wiki/Q7877","display_name":"Electrochemistry","level":3,"score":0.08883816003799438},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.0},{"id":"https://openalex.org/C111368507","wikidata":"https://www.wikidata.org/wiki/Q43518","display_name":"Oceanography","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.2202/1556-3758.1464","doi":"https://doi.org/10.2202/1556-3758.1464","is_oa":false,"landing_page_url":"https://doi.org/10.2202/1556-3758.1464","pdf_url":null,"source":{"id":"https://openalex.org/S1488397","display_name":"International Journal of Food Engineering","issn_l":"1556-3758","issn":["1556-3758","2194-5764"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310313990","host_organization_name":"De Gruyter","host_organization_lineage":["https://openalex.org/P4310313990"],"host_organization_lineage_names":["De Gruyter"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Food Engineering","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1460277358","https://openalex.org/W2067435831","https://openalex.org/W1972203718","https://openalex.org/W2093897946","https://openalex.org/W2357220461","https://openalex.org/W2146753290","https://openalex.org/W2371118502","https://openalex.org/W1444888435","https://openalex.org/W2115092158","https://openalex.org/W83785065"],"abstract_inverted_index":{"In":[0],"this":[1],"paper,":[2],"two":[3,63],"acetylcholinesterase":[4],"(AChE)":[5],"immobilization":[6,30,64,93,141,147],"methods":[7,31,142],"on":[8,58,72],"the":[9,27,67,73,84,90,96,106,111,115,126,139,144],"surface":[10,74],"of":[11,75,86,92,99,105,117],"a":[12,33,39,47],"glassy":[13],"carbon":[14],"electrode":[15],"(GCE)":[16],"were":[17,135],"compared.":[18],"AChE-employed":[19,118],"homogenate":[20],"from":[21],"chicken":[22],"brain":[23],"was":[24,56,70,123,149],"home-made":[25],"in":[26],"laboratory.":[28],"The":[29],"employed":[32],"cross-linking":[34,40,127],"method":[35,51,120,128],"with":[36,52,121,129],"glutaraldehyde":[37],"as":[38,46],"agent,":[41],"bovine":[42],"serum":[43],"albumin":[44],"(BSA)":[45],"protectant,":[48],"and":[49,133,143],"sol-gel":[50,119],"tetraethoxysilane":[53],"(TEOS).":[54],"AChE":[55,94],"immobilized":[57],"chitosan":[59],"membranes":[60],"by":[61,95,103],"these":[62],"methods.":[65],"Then":[66],"enzyme":[68],"membrane":[69],"fixed":[71],"GCE":[76],"to":[77],"prepare":[78],"an":[79],"AChE-GCE":[80],"amperometric":[81],"biosensor":[82],"for":[83,138],"detection":[85],"organophosphorus":[87],"pesticide.":[88],"Measuring":[89],"activity":[91,116],"oxidation":[97],"current":[98],"thiocholine":[100],"(TCh),":[101],"produced":[102],"hydrolysis":[104],"acetylthiocholine":[107],"iodide":[108],"(ATChI)":[109],"substrate,":[110],"results":[112],"showed":[113],"that":[114],"TEOS":[122],"higher":[124],"than":[125],"glutaraldehyde.":[130],"Biosensor":[131],"sensitivity":[132],"reproducibility":[134],"statistically":[136],"evaluated":[137],"different":[140],"most":[145],"suitable":[146],"procedure":[148],"selected.":[150]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}