{"id":"https://openalex.org/W1999546986","doi":"https://doi.org/10.1177/30.11.6183312","title":"Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies.","display_name":"Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies.","publication_year":1982,"publication_date":"1982-11-01","ids":{"openalex":"https://openalex.org/W1999546986","doi":"https://doi.org/10.1177/30.11.6183312","mag":"1999546986","pmid":"https://pubmed.ncbi.nlm.nih.gov/6183312"},"language":"en","primary_location":{"id":"doi:10.1177/30.11.6183312","doi":"https://doi.org/10.1177/30.11.6183312","is_oa":true,"landing_page_url":"https://doi.org/10.1177/30.11.6183312","pdf_url":"https://journals.sagepub.com/doi/pdf/10.1177/30.11.6183312","source":{"id":"https://openalex.org/S30841621","display_name":"Journal of Histochemistry & Cytochemistry","issn_l":"0022-1554","issn":["0022-1554","1551-5044"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Histochemistry & Cytochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://journals.sagepub.com/doi/pdf/10.1177/30.11.6183312","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109921927","display_name":"D Y Mason","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"D Y Mason","raw_affiliation_strings":["Department of i Ioleciilar lmnlunoph\u00a9ioloKy (GB.) . Pasteur Institute . Paris . France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of i Ioleciilar lmnlunoph\u00a9ioloKy (GB.) . Pasteur Institute . Paris . France","institution_ids":["https://openalex.org/I157536573"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113704290","display_name":"J L Cordell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"J L Cordell","raw_affiliation_strings":["Department of i Ioleciilar lmnlunoph\u00a9ioloKy (GB.) . Pasteur Institute . Paris . France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of i Ioleciilar lmnlunoph\u00a9ioloKy (GB.) . Pasteur Institute . Paris . France","institution_ids":["https://openalex.org/I157536573"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023629142","display_name":"Z. Abdulaziz","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Z Abdulaziz","raw_affiliation_strings":["University ofO"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University ofO","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032037036","display_name":"M Naiem","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"M Naiem","raw_affiliation_strings":["Department of i Ioleciilar lmnlunoph\u00a9ioloKy (GB.) . Pasteur Institute . Paris . France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of i Ioleciilar lmnlunoph\u00a9ioloKy (GB.) . Pasteur Institute . Paris . France","institution_ids":["https://openalex.org/I157536573"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039514855","display_name":"Guy Bordenave","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"G Bordenave","raw_affiliation_strings":["Department of i Ioleciilar lmnlunoph\u00a9ioloKy (GB.) . Pasteur Institute . Paris . France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of i Ioleciilar lmnlunoph\u00a9ioloKy (GB.) . Pasteur Institute . Paris . France","institution_ids":["https://openalex.org/I157536573"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4400,"currency":"USD","value_usd":4400},"apc_paid":null,"fwci":3.5562,"has_fulltext":true,"cited_by_count":104,"citation_normalized_percentile":{"value":0.93188983,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"30","issue":"11","first_page":"1114","last_page":"1122"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.8910999894142151,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.8910999894142151,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14134","display_name":"Classical Anatomy and Histology","score":0.024299999698996544,"subfield":{"id":"https://openalex.org/subfields/2702","display_name":"Anatomy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13921","display_name":"Biological Stains and Phytochemicals","score":0.01140000019222498,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.8560000061988831},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.5619999766349792},{"id":"https://openalex.org/keywords/storage-stability","display_name":"storage stability","score":0.4189999997615814}],"concepts":[{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.9436230659484863},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.9168031811714172},{"id":"https://openalex.org/C163864487","wikidata":"https://www.wikidata.org/wiki/Q6901461","display_name":"Monoclonal","level":4,"score":0.6448124051094055},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6262804269790649},{"id":"https://openalex.org/C162008176","wikidata":"https://www.wikidata.org/wiki/Q419305","display_name":"Peroxidase","level":3,"score":0.6187041401863098},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5630016326904297},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.5182616710662842},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5086065530776978},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.4961598813533783},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2911691963672638},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2800532579421997},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2686437964439392},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.17882171273231506},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000936","descriptor_name":"Antigen-Antibody Complex","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006735","descriptor_name":"Horseradish Peroxidase","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006825","descriptor_name":"Hybridomas","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007124","descriptor_name":"Immunoenzyme Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007146","descriptor_name":"Immunoglobulin lambda-Chains","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010544","descriptor_name":"Peroxidases","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1177/30.11.6183312","doi":"https://doi.org/10.1177/30.11.6183312","is_oa":true,"landing_page_url":"https://doi.org/10.1177/30.11.6183312","pdf_url":"https://journals.sagepub.com/doi/pdf/10.1177/30.11.6183312","source":{"id":"https://openalex.org/S30841621","display_name":"Journal of Histochemistry & Cytochemistry","issn_l":"0022-1554","issn":["0022-1554","1551-5044"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Histochemistry & Cytochemistry","raw_type":"journal-article"},{"id":"pmid:6183312","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6183312","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society","raw_type":"Comparative Study"},{"id":"pmh:oai:mouseion.jax.org:ssbb1982-4702","doi":null,"is_oa":false,"landing_page_url":"https://mouseion.jax.org/ssbb1982/3703","pdf_url":null,"source":{"id":"https://openalex.org/S4377196421","display_name":"The Mouseion at the JAXlibrary (Jackson Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210115256","host_organization_name":"Jackson Laboratory","host_organization_lineage":["https://openalex.org/I4210115256"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Subject Strain Bibliography 1982","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1177/30.11.6183312","doi":"https://doi.org/10.1177/30.11.6183312","is_oa":true,"landing_page_url":"https://doi.org/10.1177/30.11.6183312","pdf_url":"https://journals.sagepub.com/doi/pdf/10.1177/30.11.6183312","source":{"id":"https://openalex.org/S30841621","display_name":"Journal of Histochemistry & Cytochemistry","issn_l":"0022-1554","issn":["0022-1554","1551-5044"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320017","host_organization_name":"SAGE Publishing","host_organization_lineage":["https://openalex.org/P4310320017"],"host_organization_lineage_names":["SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Histochemistry & Cytochemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1999546986.pdf","grobid_xml":"https://content.openalex.org/works/W1999546986.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2078622805","https://openalex.org/W2411158748","https://openalex.org/W1990681415","https://openalex.org/W2082498125","https://openalex.org/W2113340999","https://openalex.org/W2089640207","https://openalex.org/W2030814758","https://openalex.org/W92866839","https://openalex.org/W2124314287","https://openalex.org/W1973280299"],"abstract_inverted_index":{"The":[0],"production":[1,23],"of":[2,24,31,46,76,87,102],"mouse":[3,41,51],"peroxidase:antiperoxidase":[4],"(PAP)":[5],"complexes":[6,48,54,94],"suitable":[7],"for":[8],"immunohistological":[9,63],"use":[10],"in":[11],"conjunction":[12],"with":[13,39,72],"monoclonal":[14,40,50,78,84,103],"antibodies":[15],"is":[16,86],"described.":[17],"Three":[18],"approaches":[19],"were":[20],"explored:":[21],"1)":[22],"conventional":[25,92],"polyclonal":[26],"PAP":[27,33,47,53,85,93],"complexes;":[28],"2)":[29],"conversion":[30],"rabbit":[32],"to":[34],"\"pseudo-mouse":[35],"PAP\"":[36],"by":[37,56],"incubation":[38],"anti-rabbit":[42],"immunoglobulin;":[43],"3)":[44],"formation":[45],"from":[49],"antiperoxidase.":[52],"prepared":[55],"the":[57,61,100],"latter":[58],"technique":[59],"gave":[60],"best":[62],"labeling":[64],"reactions,":[65],"being":[66],"stable":[67],"on":[68,99],"storage":[69],"and":[70],"compatible":[71],"a":[73],"wide":[74],"range":[75],"human":[77],"antibodies.":[79],"Gel":[80],"filtration":[81],"revealed":[82],"that":[83],"lower":[88],"molecular":[89],"weight":[90],"than":[91],"(fulfilling":[95],"theoretical":[96],"predictions":[97],"based":[98],"monospecificity":[101],"antibodies).":[104]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}