{"id":"https://openalex.org/W1999517832","doi":"https://doi.org/10.1158/1538-7445.am10-4024","title":"Abstract 4024: Single-allele K-ras mutation mediates CXCR4 signaling in PanIN and pancreatic cancer cells","display_name":"Abstract 4024: Single-allele K-ras mutation mediates CXCR4 signaling in PanIN and pancreatic cancer cells","publication_year":2010,"publication_date":"2010-04-01","ids":{"openalex":"https://openalex.org/W1999517832","doi":"https://doi.org/10.1158/1538-7445.am10-4024","mag":"1999517832"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am10-4024","doi":"https://doi.org/10.1158/1538-7445.am10-4024","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am10-4024","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102873675","display_name":"Xiaoming Shen","orcid":"https://orcid.org/0000-0002-5610-8702","observed_orcids":["https://orcid.org/0000-0002-5610-8702"]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiaoming Shen","raw_affiliation_strings":["1City of Hope, Duarte, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1City of Hope, Duarte, CA","institution_ids":["https://openalex.org/I1301076528"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103481303","display_name":"Jianming Lu","orcid":"https://orcid.org/0009-0001-1692-7922","observed_orcids":["https://orcid.org/0009-0001-1692-7922"]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jianming Lu","raw_affiliation_strings":["1City of Hope, Duarte, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1City of Hope, Duarte, CA","institution_ids":["https://openalex.org/I1301076528"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030087758","display_name":"Stefanie Christopher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stefanie Christopher","raw_affiliation_strings":["1City of Hope, Duarte, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1City of Hope, Duarte, CA","institution_ids":["https://openalex.org/I1301076528"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089839809","display_name":"Andrew M. Lowy","orcid":"https://orcid.org/0000-0002-7106-0735","observed_orcids":["https://orcid.org/0000-0002-7106-0735"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]},{"id":"https://openalex.org/I4390039236","display_name":"Moores Cancer Center","ror":"https://ror.org/01qkmtm61","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2800935791","https://openalex.org/I36258959","https://openalex.org/I4390039236"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew Lowy","raw_affiliation_strings":["2Division of Surgical Oncology, Moores Comprehensive Cancer Center, UCSD, Duarte, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Division of Surgical Oncology, Moores Comprehensive Cancer Center, UCSD, Duarte, CA","institution_ids":["https://openalex.org/I36258959","https://openalex.org/I4390039236"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100650735","display_name":"Joseph Kim","orcid":"https://orcid.org/0000-0001-6313-1516","observed_orcids":["https://orcid.org/0000-0001-6313-1516"]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph Kim","raw_affiliation_strings":["1City of Hope, Duarte, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1City of Hope, Duarte, CA","institution_ids":["https://openalex.org/I1301076528"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"70","issue":"8_Supplement","first_page":"4024","last_page":"4024"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11532","display_name":"Chemokine receptors and signaling","score":0.6586999893188477,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11532","display_name":"Chemokine receptors and signaling","score":0.6586999893188477,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10231","display_name":"Pancreatic and Hepatic Oncology Research","score":0.20970000326633453,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.08470000326633453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/kras-mutation","display_name":"KRAS mutation","score":0.8140000104904175},{"id":"https://openalex.org/keywords/cxcr4-signaling","display_name":"CXCR4 signaling","score":0.9319999814033508},{"id":"https://openalex.org/keywords/cxcl12","display_name":"CXCL12","score":0.9409999847412109},{"id":"https://openalex.org/keywords/pancreatic-intraepithelial-neoplasia","display_name":"pancreatic intraepithelial neoplasia","score":0.9739999771118164},{"id":"https://openalex.org/keywords/pancreatic-cancer","display_name":"pancreatic cancer","score":0.9879999756813049},{"id":"https://openalex.org/keywords/erk1-2-phosphorylation","display_name":"ERK1/2 phosphorylation","score":0.8809999823570251},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.7940000295639038},{"id":"https://openalex.org/keywords/mapk-signaling","display_name":"MAPK signaling","score":0.6970000267028809},{"id":"https://openalex.org/keywords/pi-3k-akt-pathway","display_name":"PI-3K/Akt pathway","score":0.9129999876022339},{"id":"https://openalex.org/keywords/chemokine-receptor-signaling","display_name":"chemokine receptor signaling","score":0.574999988079071}],"concepts":[{"id":"https://openalex.org/C2780210213","wikidata":"https://www.wikidata.org/wiki/Q212961","display_name":"Pancreatic cancer","level":3,"score":0.7077354192733765},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.5565862059593201},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5068774819374084},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.5019304752349854},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.49653297662734985},{"id":"https://openalex.org/C129470790","wikidata":"https://www.wikidata.org/wiki/Q415718","display_name":"CXCR4","level":4,"score":0.4873109757900238},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.46475544571876526},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4478793144226074},{"id":"https://openalex.org/C2909925829","wikidata":"https://www.wikidata.org/wiki/Q212961","display_name":"Pancreatic Intraepithelial Neoplasia","level":5,"score":0.440701961517334},{"id":"https://openalex.org/C57074206","wikidata":"https://www.wikidata.org/wiki/Q14197552","display_name":"MAPK/ERK pathway","level":3,"score":0.4339749217033386},{"id":"https://openalex.org/C75217442","wikidata":"https://www.wikidata.org/wiki/Q423650","display_name":"Protein kinase B","level":3,"score":0.41183996200561523},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.4018595218658447},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.3306775987148285},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2001938819885254},{"id":"https://openalex.org/C13373296","wikidata":"https://www.wikidata.org/wiki/Q421866","display_name":"Chemokine","level":3,"score":0.139664888381958},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07180854678153992},{"id":"https://openalex.org/C2992026798","wikidata":"https://www.wikidata.org/wiki/Q212961","display_name":"Pancreatic ductal adenocarcinoma","level":4,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am10-4024","doi":"https://doi.org/10.1158/1538-7445.am10-4024","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am10-4024","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9391000270843506}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2142761194","https://openalex.org/W2010816322","https://openalex.org/W2022979958","https://openalex.org/W2090950902","https://openalex.org/W3089435639","https://openalex.org/W2168207107","https://openalex.org/W2051473652","https://openalex.org/W2330173317","https://openalex.org/W1773289659","https://openalex.org/W3127622980"],"abstract_inverted_index":{"Abstract":[0],"Single-allele":[1],"K-ras":[2,89,102,108,115,131,182,205,248,268,287,289,337,370],"mutation":[3,206],"mediates":[4,371],"CXCR4":[5,49,78,91,136,161,348,365],"signaling":[6,317],"in":[7,56,90,140,153,208,245,283,291,374,382],"PanIN":[8,124,353],"and":[9,53,61,104,110,112,117,137,179,196,201,222,258,265,298,305,354,377],"pancreatic":[10,57,63,96,355],"cancer":[11,64,97,356],"cells":[12,219,224,234,249,296,326],"Xiaoming":[13],"Shen,":[14],"Jianming":[15],"Lu,":[16],"Stefanie":[17],"Christopher,":[18],"Andrew":[19],"M.":[20],"Lowy,":[21],"Joseph":[22],"Kim":[23],"Department":[24],"of":[25,29,32,87,194,254,303,310,314,334,352,393,398],"Oncologic":[26],"Surgery,":[27],"City":[28],"Hope":[30],"Division":[31],"Surgical":[33],"Oncology,":[34],"Moores":[35],"Comprehensive":[36],"Cancer":[37,403,413],"Center,":[38],"UCSD":[39],"Introduction:":[40],"We":[41,358],"previously":[42],"demonstrated":[43],"that":[44,217,312,343,361],"the":[45,85,142,159,175,210,252,271,284,292,344,350,378,394,399],"activated":[46,345],"chemokine":[47,346],"receptor":[48,347],"signals":[50],"through":[51],"MAPK":[52],"PI-3K/AKT":[54],"pathways":[55],"intraepithelial":[58],"neoplasia":[59],"(PanIN)":[60],"invasive":[62],"to":[65,83,187,237,327],"enhance":[66],"cell":[67,98,125,143,197,211,307,383],"proliferation.":[68,198,308,384],"It":[69],"remains":[70],"unclear":[71],"whether":[72],"mutant-derived":[73],"constitutive":[74],"K-Ras":[75,138,154,178,221,243,273,316,363,375],"activity":[76,155,244,274,376],"regulates":[77],"signaling.":[79],"Our":[80,340],"aim":[81],"was":[82,132,185,280],"characterize":[84,174],"role":[86],"mutant":[88,107,114,130,230,247,267,286,295,369],"signal":[92,321],"transduction.":[93],"Methods:":[94],"Human":[95],"lines":[99,144],"with":[100,127,276],"wild-type":[101,264],"(BxPC-3":[103],"Hs766T),":[105],"single-allele":[106,246,285,368],"(PANC-1":[109,297],"FG),":[111],"double-allele":[113,266],"(AsPC-1":[116],"MIAPaCa-2)":[118],"were":[119,145,163,235],"evaluated.":[120],"An":[121],"established":[122],"murine":[123],"line":[126],"single":[128,293],"allele":[129,294],"also":[133,324,359],"assessed.":[134],"First,":[135],"expression":[139],"all":[141,209,218,223],"measured":[146,164],"by":[147,157,165],"Western":[148,213],"blot":[149,214],"assay.":[150,171],"Then,":[151],"changes":[152],"induced":[156,332],"CXCL12,":[158,238],"selective":[160],"ligand,":[162],"a":[166,181],"Raf-Ras":[167],"binding":[168],"domain":[169],"ELISA":[170],"To":[172],"further":[173],"interaction":[176],"between":[177],"CXCR4,":[180,226],"knockdown":[183,290,313],"assay":[184,215],"used":[186],"examine":[188],"its":[189],"effects":[190],"on":[191],"CXCL12-mediated":[192],"activation":[193],"ERK1/2":[195,304,335],"Results:":[199],"PCR":[200],"DNA":[202],"sequencing":[203],"verified":[204],"status":[207],"lines.":[212],"showed":[216],"expressed":[220,225],"except":[227],"MIAPaCa-2":[228],"(double-allele":[229],"K-ras).":[231],"When":[232],"CXCR4-expressing":[233],"exposed":[236,325],"we":[239,323],"observed":[240,282],"significantly":[241],"increased":[242,272],"(what":[250],"is":[251],"magnitude":[253],"change?111%":[255],"for":[256,260,402],"panIN":[257],"18.6%":[259],"panc1),":[261],"but":[262],"not":[263],"cells.":[269,288,357],"Furthermore,":[270],"corresponded":[275],"CXCL12-induced":[277,301],"proliferation,":[278],"which":[279,331],"only":[281,367],"PanIN)":[299],"blocked":[300],"phosphorylation":[302,333],"abrogated":[306],"Because":[309],"concern":[311],"dominant":[315],"may":[318],"block":[319],"receptor-mediated":[320],"transduction,":[322],"epidermal":[328],"growth":[329],"factor,":[330],"despite":[336],"knockdown.":[338],"Conclusion:":[339],"results":[341],"verify":[342],"promotes":[349],"proliferation":[351],"show":[360],"while":[362],"conducts":[364],"signals,":[366],"CXCR4-induced":[372],"increases":[373],"subsequent":[379],"downstream":[380],"enhancement":[381],"Citation":[385],"Format:":[386],"{Authors}.":[387],"{Abstract":[388],"title}":[389],"[abstract].":[390],"In:":[391],"Proceedings":[392],"101st":[395],"Annual":[396],"Meeting":[397],"American":[400],"Association":[401],"Research;":[404],"2010":[405],"Apr":[406],"17-21;":[407],"Washington,":[408],"DC.":[409],"Philadelphia":[410],"(PA):":[411],"AACR;":[412],"Res":[414],"2010;70(8":[415],"Suppl):Abstract":[416],"nr":[417],"4024.":[418]},"counts_by_year":[],"updated_date":"2026-10-27T15:51:10.671819","created_date":"2016-06-24T00:00:00"}