{"id":"https://openalex.org/W1999101059","doi":"https://doi.org/10.1111/j.1399-3054.2006.00811.x","title":"Biochemical and molecular characterizations of nicotine demethylase in tobacco","display_name":"Biochemical and molecular characterizations of nicotine demethylase in tobacco","publication_year":2006,"publication_date":"2006-12-21","ids":{"openalex":"https://openalex.org/W1999101059","doi":"https://doi.org/10.1111/j.1399-3054.2006.00811.x","mag":"1999101059"},"language":"en","primary_location":{"id":"doi:10.1111/j.1399-3054.2006.00811.x","doi":"https://doi.org/10.1111/j.1399-3054.2006.00811.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1399-3054.2006.00811.x","pdf_url":null,"source":{"id":"https://openalex.org/S185046733","display_name":"Physiologia Plantarum","issn_l":"0031-9317","issn":["0031-9317","1399-3054"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Physiologia Plantarum","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000168447","display_name":"Dongmei Xu","orcid":"https://orcid.org/0000-0001-6925-0727","observed_orcids":["https://orcid.org/0000-0001-6925-0727"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Dongmei Xu","raw_affiliation_strings":["GenApps Inc., 4262 Colby Road, Winchester, KY 40391, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GenApps Inc., 4262 Colby Road, Winchester, KY 40391, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039581923","display_name":"Yanxin Shen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yanxin Shen","raw_affiliation_strings":["GenApps Inc., 4262 Colby Road, Winchester, KY 40391, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GenApps Inc., 4262 Colby Road, Winchester, KY 40391, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054984493","display_name":"Joe Chappell","orcid":"https://orcid.org/0000-0001-8469-8976","observed_orcids":["https://orcid.org/0000-0001-8469-8976"]},"institutions":[{"id":"https://openalex.org/I143302722","display_name":"University of Kentucky","ror":"https://ror.org/02k3smh20","country_code":"US","type":"education","lineage":["https://openalex.org/I143302722"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joe Chappell","raw_affiliation_strings":["Plant Biology Program, University of Kentucky, Lexington, KY 40546-0312, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Biology Program, University of Kentucky, Lexington, KY 40546-0312, USA","institution_ids":["https://openalex.org/I143302722"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078362249","display_name":"Mingwu Cui","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mingwu Cui","raw_affiliation_strings":["GenApps Inc., 4262 Colby Road, Winchester, KY 40391, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GenApps Inc., 4262 Colby Road, Winchester, KY 40391, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102188670","display_name":"Mark T. Nielsen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mark Nielsen","raw_affiliation_strings":["GenApps Inc., 4262 Colby Road, Winchester, KY 40391, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GenApps Inc., 4262 Colby Road, Winchester, KY 40391, USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5000168447"],"corresponding_institution_ids":[],"apc_list":{"value":4150,"currency":"USD","value_usd":4150},"apc_paid":null,"fwci":0.8105,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.67140425,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"129","issue":"2","first_page":"307","last_page":"319"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9150000214576721,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9150000214576721,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.03849999979138374,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11237","display_name":"Plant Gene Expression Analysis","score":0.016499999910593033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cytochrome-p450","display_name":"cytochrome P450","score":0.9010000228881836},{"id":"https://openalex.org/keywords/nicotiana-tabacum","display_name":"tobacco","score":0.9190000295639038},{"id":"https://openalex.org/keywords/nornicotine","display_name":"nornicotine","score":0.9430000185966492},{"id":"https://openalex.org/keywords/nnn","display_name":"NNN","score":0.5400000214576721},{"id":"https://openalex.org/keywords/nitrosamines","display_name":"nitrosamines","score":0.7089999914169312},{"id":"https://openalex.org/keywords/ethylene-induction","display_name":"ethylene induction","score":0.8040000200271606},{"id":"https://openalex.org/keywords/microsomal-enzyme-activity","display_name":"microsomal enzyme activity","score":0.7699999809265137},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.5630000233650208},{"id":"https://openalex.org/keywords/cdna-expression","display_name":"cDNA expression","score":0.5299999713897705},{"id":"https://openalex.org/keywords/yeast-overexpression","display_name":"yeast overexpression","score":0.6859999895095825},{"id":"https://openalex.org/keywords/active-site-residues","display_name":"active site residues","score":0.8220000267028809},{"id":"https://openalex.org/keywords/molecular-modeling","display_name":"molecular modeling","score":0.8140000104904175},{"id":"https://openalex.org/keywords/tobacco-alkaloids","display_name":"tobacco alkaloids","score":0.5830000042915344}],"concepts":[{"id":"https://openalex.org/C2776166849","wikidata":"https://www.wikidata.org/wiki/Q907939","display_name":"Nornicotine","level":3,"score":0.815567135810852},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.6609962582588196},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.651364803314209},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5701801180839539},{"id":"https://openalex.org/C2781205006","wikidata":"https://www.wikidata.org/wiki/Q181095","display_name":"Nicotiana tabacum","level":3,"score":0.47998496890068054},{"id":"https://openalex.org/C2781364455","wikidata":"https://www.wikidata.org/wiki/Q2531584","display_name":"Demethylase","level":4,"score":0.47119686007499695},{"id":"https://openalex.org/C526171541","wikidata":"https://www.wikidata.org/wiki/Q407693","display_name":"Cytochrome P450","level":3,"score":0.4398869276046753},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4272100329399109},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.40699249505996704},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3309869170188904},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3284537196159363},{"id":"https://openalex.org/C2779547902","wikidata":"https://www.wikidata.org/wiki/Q28086552","display_name":"Nicotine","level":2,"score":0.3202522099018097},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.0},{"id":"https://openalex.org/C41091548","wikidata":"https://www.wikidata.org/wiki/Q26939","display_name":"Epigenetics","level":3,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1111/j.1399-3054.2006.00811.x","doi":"https://doi.org/10.1111/j.1399-3054.2006.00811.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1399-3054.2006.00811.x","pdf_url":null,"source":{"id":"https://openalex.org/S185046733","display_name":"Physiologia Plantarum","issn_l":"0031-9317","issn":["0031-9317","1399-3054"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Physiologia Plantarum","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Good health and well-being","score":0.8399999737739563,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320308590","display_name":"University of California","ror":"https://ror.org/00pjdza24"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":55,"referenced_works":["https://openalex.org/W121749493","https://openalex.org/W264166021","https://openalex.org/W307598957","https://openalex.org/W1498652161","https://openalex.org/W1511455152","https://openalex.org/W1515404266","https://openalex.org/W1520292993","https://openalex.org/W1553682840","https://openalex.org/W1792722883","https://openalex.org/W1833106351","https://openalex.org/W1951469402","https://openalex.org/W1969433120","https://openalex.org/W1970868368","https://openalex.org/W1992187482","https://openalex.org/W1999494471","https://openalex.org/W2000676037","https://openalex.org/W2002010793","https://openalex.org/W2006639378","https://openalex.org/W2008180436","https://openalex.org/W2011826075","https://openalex.org/W2014685913","https://openalex.org/W2014919794","https://openalex.org/W2026361884","https://openalex.org/W2031804135","https://openalex.org/W2033048136","https://openalex.org/W2038973920","https://openalex.org/W2051937797","https://openalex.org/W2052704239","https://openalex.org/W2065024025","https://openalex.org/W2069885590","https://openalex.org/W2076780060","https://openalex.org/W2079038249","https://openalex.org/W2087358933","https://openalex.org/W2090294719","https://openalex.org/W2096443964","https://openalex.org/W2098127960","https://openalex.org/W2107091285","https://openalex.org/W2111329465","https://openalex.org/W2119271777","https://openalex.org/W2123964275","https://openalex.org/W2133986791","https://openalex.org/W2141358713","https://openalex.org/W2148032599","https://openalex.org/W2155471967","https://openalex.org/W2156191061","https://openalex.org/W2156985978","https://openalex.org/W2159686532","https://openalex.org/W2166460120","https://openalex.org/W2493442865","https://openalex.org/W2554486018","https://openalex.org/W2804007336","https://openalex.org/W3112302982","https://openalex.org/W4240181774","https://openalex.org/W4285719527","https://openalex.org/W6787786268"],"related_works":["https://openalex.org/W2516820399","https://openalex.org/W2065024025","https://openalex.org/W4401560685","https://openalex.org/W2007407648","https://openalex.org/W2014120943","https://openalex.org/W2006899454","https://openalex.org/W2075690206","https://openalex.org/W2079994720","https://openalex.org/W2150920296","https://openalex.org/W2119271777"],"abstract_inverted_index":{"Plant":[0],"cytochrome":[1],"P450":[2,142,155],"(CYP)":[3],"enzymes":[4],"are":[5],"involved":[6],"in":[7,32,77,194],"the":[8,44,65,81,174,204],"biosynthesis":[9],"of":[10,48,91,112,126,178],"many":[11],"primary":[12],"and":[13,23,46,88,100,106,144,160,184,243],"secondary":[14],"metabolites":[15],"including":[16],"phenylpropanoids,":[17],"alkaloids,":[18],"terpenoids,":[19],"lipids,":[20],"cyanogenic":[21],"glycosides,":[22],"glucosinolates.":[24],"However,":[25],"while":[26],"hundreds":[27],"ofCYPgenes":[28],"have":[29,37],"been":[30,38],"identified":[31],"plant":[33,198],"genomes,":[34],"relatively":[35],"few":[36],"functionally":[39],"characterized.":[40],"We":[41],"report":[42],"here":[43],"cloning":[45],"characterization":[47],"a":[49,62,107,130],"CYP":[50],"enzyme":[51],"from":[52,146,201],"tobacco":[53],"(Nicotiana":[54],"tabacumL.)":[55],"that":[56,215,230],"demethylates":[57],"nicotine":[58,105],"to":[59,64,74,140,190,229,237,246],"form":[60,90],"nornicotine,":[61],"precursor":[63],"nitrosamineN\u2032\u2010nitrosonornicotine":[66],"(NNN).":[67],"Microsomal":[68],"demethylase":[69,249],"activity":[70,119,227],"was":[71,120,235],"first":[72],"shown":[73],"be":[75,191],"induced":[76],"leaves":[78],"treated":[79],"with":[80,136,209],"growth":[82],"regulator":[83],"ethylene,":[84],"required":[85],"molecular":[86],"oxygen":[87],"reduced":[89,121],"nicotinamide":[92],"adenine":[93],"dinucleotide":[94],"phosphate":[95],"(NADPH)":[96],"for":[97,225,244],"full":[98],"activity,":[99],"exhibited":[101],"aKmof":[102],"3.9":[103],"\u03bcMfor":[104],"maximum":[108],"turnover":[109],"rate":[110],"(Vmax)":[111],"8.9":[113],"pkat":[114],"mg\u22121protein.":[115],"Equally":[116],"important,":[117],"microsomal":[118],"greatly":[122],"by":[123],"several":[124],"inhibitors":[125],"CYP\u2010mediated":[127],"reactions.":[128],"Using":[129],"polymerase":[131],"chain":[132],"reaction":[133],"based":[134],"strategy":[135],"degenerate":[137],"primers":[138],"designed":[139],"conserve":[141],"motifs":[143],"mRNA":[145,176],"ethylene\u2010treated":[147,195],"leaves,":[148],"putative":[149,239,248],"gene":[150,156],"fragments":[151],"representing":[152],"32":[153],"different":[154],"families":[157],"were":[158,164,188],"isolated":[159],"numerous":[161],"full\u2010length":[162,182,206],"genes":[163],"cloned.":[165],"Employing":[166],"GeneChip\u00ae(Affymetrix":[167],"Inc.,":[168],"Santa":[169],"Clara,":[170],"CA)":[171],"microarray":[172],"hybridizations,":[173],"steady\u2010state":[175],"level":[177],"two":[179,205,210],"highly":[180,192,212],"related":[181],"cDNAs":[183,207],"one":[185,217],"cDNA":[186],"fragment":[187],"demonstrated":[189,214],"expressed":[193],"vs.":[196],"control":[197],"material.":[199],"Microsomes":[200],"yeast":[202],"over\u2010expressing":[203],"along":[208],"other":[211,247],"homologousP450genes":[213],"only":[216],"cDNA,":[218],"D121\u2010AA8":[219],"(GenBank":[220],"accession":[221],"no.":[222],"DQ205656)":[223],"encoded":[224],"demethylation":[226],"comparable":[228],"foundin":[231],"planta.":[232],"Molecular":[233],"modeling":[234],"used":[236],"identify":[238],"active":[240],"site":[241],"residues":[242],"comparisons":[245],"cDNAs.":[250]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":5}],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2016-06-24T00:00:00"}