{"id":"https://openalex.org/W1998505969","doi":"https://doi.org/10.1016/0003-2697(88)90289-8","title":"High-yield and high-degree purification of human \u03b1-fetoprotein produced by adaptation of the human hepatoma cell line Hep G2 in a serum-free medium","display_name":"High-yield and high-degree purification of human \u03b1-fetoprotein produced by adaptation of the human hepatoma cell line Hep G2 in a serum-free medium","publication_year":1988,"publication_date":"1988-03-01","ids":{"openalex":"https://openalex.org/W1998505969","doi":"https://doi.org/10.1016/0003-2697(88)90289-8","mag":"1998505969","pmid":"https://pubmed.ncbi.nlm.nih.gov/2454595"},"language":"en","primary_location":{"id":"doi:10.1016/0003-2697(88)90289-8","doi":"https://doi.org/10.1016/0003-2697(88)90289-8","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(88)90289-8","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015222078","display_name":"Mario Felice Tecce","orcid":"https://orcid.org/0000-0003-2355-6047","observed_orcids":["https://orcid.org/0000-0003-2355-6047"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mario F. Tecce","raw_affiliation_strings":["Laboratory of Cell Biology, Sclavo Research Center, Siena, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Cell Biology, Sclavo Research Center, Siena, Italy","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037191738","display_name":"B Terrana","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102064193","display_name":"University of Siena","ror":"https://ror.org/01tevnk56","country_code":"IT","type":"education","lineage":["https://openalex.org/I102064193"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Benedetto Terrana","raw_affiliation_strings":["Laboratory of Cell Biology, Sclavo Research Center, Via Fiorentina 1, 53100 Siena, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Cell Biology, Sclavo Research Center, Via Fiorentina 1, 53100 Siena, Italy","institution_ids":["https://openalex.org/I102064193"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":1.0034,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.74189662,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"169","issue":"2","first_page":"306","last_page":"311"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.4560999870300293,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.4560999870300293,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.4180000126361847,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10073","display_name":"Hepatocellular Carcinoma Treatment and Prognosis","score":0.04129999876022339,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-fetoprotein","display_name":"alpha-fetoprotein","score":0.9480000138282776},{"id":"https://openalex.org/keywords/hepg2-cells","display_name":"HepG2 cells","score":0.9020000100135803},{"id":"https://openalex.org/keywords/hepatoma-cell-lines","display_name":"hepatoma cell lines","score":0.7149999737739563},{"id":"https://openalex.org/keywords/serum-free-medium","display_name":"serum-free medium","score":0.8349999785423279},{"id":"https://openalex.org/keywords/albumin","display_name":"albumin","score":0.6039999723434448},{"id":"https://openalex.org/keywords/protein-secretion","display_name":"protein secretion","score":0.628000020980835},{"id":"https://openalex.org/keywords/preparative-electrophoresis","display_name":"preparative electrophoresis","score":0.8989999890327454},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.8859999775886536},{"id":"https://openalex.org/keywords/immunoassay-standardization","display_name":"immunoassay standardization","score":0.7080000042915344},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7129999995231628}],"concepts":[{"id":"https://openalex.org/C2780624289","wikidata":"https://www.wikidata.org/wiki/Q306076","display_name":"Alpha-fetoprotein","level":3,"score":0.7532957792282104},{"id":"https://openalex.org/C2776125364","wikidata":"https://www.wikidata.org/wiki/Q169219","display_name":"Albumin","level":2,"score":0.6337664723396301},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5103394389152527},{"id":"https://openalex.org/C9893847","wikidata":"https://www.wikidata.org/wiki/Q1425625","display_name":"Reproducibility","level":2,"score":0.44929543137550354},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4288058280944824},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42301642894744873},{"id":"https://openalex.org/C86807702","wikidata":"https://www.wikidata.org/wiki/Q2620381","display_name":"Bovine serum albumin","level":2,"score":0.41492271423339844},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.41385072469711304},{"id":"https://openalex.org/C64943373","wikidata":"https://www.wikidata.org/wiki/Q2651003","display_name":"Alpha (finance)","level":4,"score":0.4129564166069031},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3785371780395508},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3700519800186157},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.09373852610588074},{"id":"https://openalex.org/C49453240","wikidata":"https://www.wikidata.org/wiki/Q1592163","display_name":"Construct validity","level":3,"score":0.0},{"id":"https://openalex.org/C2778019345","wikidata":"https://www.wikidata.org/wiki/Q1148337","display_name":"Hepatocellular carcinoma","level":2,"score":0.0},{"id":"https://openalex.org/C2775944032","wikidata":"https://www.wikidata.org/wiki/Q22907659","display_name":"Patient satisfaction","level":2,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.0},{"id":"https://openalex.org/C159110408","wikidata":"https://www.wikidata.org/wiki/Q121176","display_name":"Nursing","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000220","descriptor_name":"Adaptation, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000509","descriptor_name":"alpha-Fetoproteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000509","descriptor_name":"alpha-Fetoproteins","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006528","descriptor_name":"Carcinoma, Hepatocellular","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008113","descriptor_name":"Liver Neoplasms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0003-2697(88)90289-8","doi":"https://doi.org/10.1016/0003-2697(88)90289-8","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(88)90289-8","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:2454595","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2454595","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8560000061988831,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1507890231","https://openalex.org/W1545934301","https://openalex.org/W1968834637","https://openalex.org/W1969699638","https://openalex.org/W1970126718","https://openalex.org/W1978818954","https://openalex.org/W1981815989","https://openalex.org/W2001774406","https://openalex.org/W2011864574","https://openalex.org/W2039892671","https://openalex.org/W2052425036","https://openalex.org/W2072167444","https://openalex.org/W2072585523","https://openalex.org/W2087062084","https://openalex.org/W2100837269","https://openalex.org/W2396847640","https://openalex.org/W4313313416"],"related_works":["https://openalex.org/W2082597539","https://openalex.org/W2413616916","https://openalex.org/W2396847640","https://openalex.org/W2412362663","https://openalex.org/W2043464257","https://openalex.org/W2324506336","https://openalex.org/W1599866342","https://openalex.org/W2076949661","https://openalex.org/W7067856921","https://openalex.org/W2156658561"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}