{"id":"https://openalex.org/W1998225673","doi":"https://doi.org/10.1111/jam.12206","title":"Evaluation of DNA extraction methods for Bacillus anthracis spores isolated from spiked food samples","display_name":"Evaluation of DNA extraction methods for Bacillus anthracis spores isolated from spiked food samples","publication_year":2013,"publication_date":"2013-04-06","ids":{"openalex":"https://openalex.org/W1998225673","doi":"https://doi.org/10.1111/jam.12206","mag":"1998225673","pmid":"https://pubmed.ncbi.nlm.nih.gov/23560745"},"language":"en","primary_location":{"id":"doi:10.1111/jam.12206","doi":"https://doi.org/10.1111/jam.12206","is_oa":false,"landing_page_url":"https://doi.org/10.1111/jam.12206","pdf_url":null,"source":{"id":"https://openalex.org/S39794811","display_name":"Journal of Applied Microbiology","issn_l":"1364-5072","issn":["1364-5072","1365-2672"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://open-science.canada.ca/handle/123456789/4508","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"M.C. Thomas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1307613440","display_name":"Canadian Food Inspection Agency","ror":"https://ror.org/00qxr8t08","country_code":"CA","type":"government","lineage":["https://openalex.org/I1307613440"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"M.C. Thomas","raw_affiliation_strings":["Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada","institution_ids":["https://openalex.org/I1307613440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018144789","display_name":"Michael J. Shields","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1307613440","display_name":"Canadian Food Inspection Agency","ror":"https://ror.org/00qxr8t08","country_code":"CA","type":"government","lineage":["https://openalex.org/I1307613440"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"M.J. Shields","raw_affiliation_strings":["Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada","institution_ids":["https://openalex.org/I1307613440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045054959","display_name":"Kristen R. Hahn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1307613440","display_name":"Canadian Food Inspection Agency","ror":"https://ror.org/00qxr8t08","country_code":"CA","type":"government","lineage":["https://openalex.org/I1307613440"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"K.R. Hahn","raw_affiliation_strings":["Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada","institution_ids":["https://openalex.org/I1307613440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032571030","display_name":"Timothy W. Janzen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1307613440","display_name":"Canadian Food Inspection Agency","ror":"https://ror.org/00qxr8t08","country_code":"CA","type":"government","lineage":["https://openalex.org/I1307613440"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"T.W. Janzen","raw_affiliation_strings":["Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada","institution_ids":["https://openalex.org/I1307613440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008152462","display_name":"Noriko Goji","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1307613440","display_name":"Canadian Food Inspection Agency","ror":"https://ror.org/00qxr8t08","country_code":"CA","type":"government","lineage":["https://openalex.org/I1307613440"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"N. Goji","raw_affiliation_strings":["Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada","institution_ids":["https://openalex.org/I1307613440"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108917647","display_name":"Kingsley Kwabena Amoako","orcid":"https://orcid.org/0009-0004-7398-1732","observed_orcids":["https://orcid.org/0009-0004-7398-1732"]},"institutions":[{"id":"https://openalex.org/I1307613440","display_name":"Canadian Food Inspection Agency","ror":"https://ror.org/00qxr8t08","country_code":"CA","type":"government","lineage":["https://openalex.org/I1307613440"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"K.K. Amoako","raw_affiliation_strings":["Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Canadian Food Inspection Agency, National Centers for Animal Disease, Lethbridge Laboratory, Lethbridge, AB, Canada","institution_ids":["https://openalex.org/I1307613440"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5108917647"],"corresponding_institution_ids":["https://openalex.org/I1307613440"],"apc_list":{"value":2864,"currency":"GBP","value_usd":3777},"apc_paid":null,"fwci":1.3107,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.78181184,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"115","issue":"1","first_page":"156","last_page":"162"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11515","display_name":"Bacillus and Francisella bacterial research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11515","display_name":"Bacillus and Francisella bacterial research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacillus-anthracis","display_name":"Bacillus anthracis","score":0.9369999766349792},{"id":"https://openalex.org/keywords/bacterial-spores","display_name":"bacterial spores","score":0.796999990940094},{"id":"https://openalex.org/keywords/dna-extraction-kits","display_name":"DNA extraction kits","score":0.7639999985694885},{"id":"https://openalex.org/keywords/food-contamination","display_name":"food contamination","score":0.5559999942779541},{"id":"https://openalex.org/keywords/immunomagnetic-separation","display_name":"immunomagnetic separation","score":0.9760000109672546},{"id":"https://openalex.org/keywords/pcr-inhibitors","display_name":"PCR inhibitors","score":0.6169999837875366},{"id":"https://openalex.org/keywords/foodborne-pathogens","display_name":"foodborne pathogens","score":0.47999998927116394},{"id":"https://openalex.org/keywords/apple-juice","display_name":"apple juice","score":0.6539999842643738},{"id":"https://openalex.org/keywords/ham","display_name":"ham","score":0.9909999966621399},{"id":"https://openalex.org/keywords/whole-milk","display_name":"whole milk","score":0.9610000252723694}],"concepts":[{"id":"https://openalex.org/C2780239966","wikidata":"https://www.wikidata.org/wiki/Q614156","display_name":"Bacillus anthracis","level":3,"score":0.9113191962242126},{"id":"https://openalex.org/C174618031","wikidata":"https://www.wikidata.org/wiki/Q177332","display_name":"Spore","level":2,"score":0.8515685796737671},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.6835207939147949},{"id":"https://openalex.org/C2781053203","wikidata":"https://www.wikidata.org/wiki/Q1660182","display_name":"Immunomagnetic separation","level":2,"score":0.5615705251693726},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5539400577545166},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5494146943092346},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.4789622128009796},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.4643087387084961},{"id":"https://openalex.org/C184112429","wikidata":"https://www.wikidata.org/wiki/Q476174","display_name":"Serial dilution","level":3,"score":0.4125211536884308},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4064880609512329},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.354009211063385},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.3054259717464447},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.30325138568878174},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0845896303653717},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.06101897358894348},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001408","descriptor_name":"Bacillus anthracis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D005516","descriptor_name":"Food Microbiology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013171","descriptor_name":"Spores, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018189","descriptor_name":"Immunomagnetic Separation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1111/jam.12206","doi":"https://doi.org/10.1111/jam.12206","is_oa":false,"landing_page_url":"https://doi.org/10.1111/jam.12206","pdf_url":null,"source":{"id":"https://openalex.org/S39794811","display_name":"Journal of Applied Microbiology","issn_l":"1364-5072","issn":["1364-5072","1365-2672"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Microbiology","raw_type":"journal-article"},{"id":"pmid:23560745","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23560745","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of applied microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:open-science.canada.ca:123456789/4508","doi":null,"is_oa":true,"landing_page_url":"https://open-science.canada.ca/handle/123456789/4508","pdf_url":null,"source":{"id":"https://openalex.org/S7407055455","display_name":"Federal Open Science Repository of Canada / Le D\u00e9p\u00f4t f\u00e9d\u00e9ral de science ouverte du Canada","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"}],"best_oa_location":{"id":"pmh:oai:open-science.canada.ca:123456789/4508","doi":null,"is_oa":true,"landing_page_url":"https://open-science.canada.ca/handle/123456789/4508","pdf_url":null,"source":{"id":"https://openalex.org/S7407055455","display_name":"Federal Open Science Repository of Canada / Le D\u00e9p\u00f4t f\u00e9d\u00e9ral de science ouverte du Canada","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.5666999816894531},{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4000000059604645}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W167946898","https://openalex.org/W1558884242","https://openalex.org/W1571616236","https://openalex.org/W1964105560","https://openalex.org/W2017666284","https://openalex.org/W2034764628","https://openalex.org/W2042947048","https://openalex.org/W2050550651","https://openalex.org/W2080072512","https://openalex.org/W2080309005","https://openalex.org/W2081790467","https://openalex.org/W2083067586","https://openalex.org/W2097492854","https://openalex.org/W2101385400","https://openalex.org/W2103144398","https://openalex.org/W2105697947","https://openalex.org/W2109599865","https://openalex.org/W2118537438","https://openalex.org/W2154586372","https://openalex.org/W2163651757","https://openalex.org/W2327487288","https://openalex.org/W4251880970"],"related_works":["https://openalex.org/W2003872518","https://openalex.org/W3046579361","https://openalex.org/W2081790467","https://openalex.org/W2489550915","https://openalex.org/W2592436970","https://openalex.org/W2017666284","https://openalex.org/W1571616236","https://openalex.org/W3026576446","https://openalex.org/W1719533312","https://openalex.org/W2462850794"],"abstract_inverted_index":{"AIMS:":[0],"Nine":[1],"commercial":[2,180],"DNA":[3,12,56,112,181],"extraction":[4,182,203],"kits":[5,28],"were":[6,48,158],"evaluated":[7],"for":[8,51,212],"the":[9,34,191],"isolation":[10],"of":[11,17,60,79,116,163,168,218,221],"from":[13,57,113,121,127,136,173,225],"10-fold":[14],"serial":[15],"dilutions":[16],"Bacillus":[18],"anthracis":[19,62,118,170,214,223],"spores":[20,63,119,171,224],"using":[21],"quantitative":[22],"real-time":[23],"PCR":[24,164],"(qPCR).":[25],"The":[26,105,202],"three":[27],"determined":[29],"by":[30,175],"qPCR":[31],"to":[32,54,160],"yield":[33],"most":[35],"sensitive":[36,185],"and":[37,68,93,96,109,133,149,155,215],"consistent":[38],"detection":[39,217],"(Epicenter":[40],"MasterPure":[41,106,192],"Gram":[42,193],"Positive;":[43],"MoBio":[44],"PowerFood;":[45],"ABI":[46],"PrepSeq)":[47],"subsequently":[49],"tested":[50],"their":[52],"ability":[53],"isolate":[55],"trace":[58],"amounts":[59,115],"B.":[61,117,169,213,222],"(approx.":[64],"6\u00b75":[65],"\u00d7":[66,70],"10(1)":[67],"1\u00b73":[69],"10(2)":[71],"CFU":[72],"in":[73],"25":[74],"ml":[75],"or":[76],"50":[77],"g":[78],"food":[80,85,122,174,227],"sample)":[81],"spiked":[82],"into":[83],"complex":[84],"samples":[86,157],"including":[87],"apple":[88,128],"juice,":[89,129],"ham,":[90,130],"whole":[91,131],"milk":[92,132],"bagged":[94,134],"salad":[95,135],"recovered":[97],"with":[98,190,208],"immunomagnetic":[99],"separation":[100],"(IMS).":[101],"METHODS":[102],"AND":[103,197],"RESULTS:":[104],"kit":[107],"effectively":[108],"consistently":[110],"isolated":[111,172],"low":[114,219],"captured":[120],"samples.":[123,228],"Detection":[124,167],"was":[125],"achieved":[126],"as":[137,139],"few":[138],"65":[140],"\u00b1":[141,144,147,151],"14,":[142],"68":[143],"8,":[145],"66":[146],"4":[148],"52":[150],"16":[152],"CFU,":[153],"respectively,":[154],"IMS":[156,176,209],"demonstrated":[159],"be":[161,188],"free":[162],"inhibitors.":[165],"CONCLUSIONS:":[166],"differs":[177],"substantially":[178],"between":[179],"kits;":[183],"however,":[184],"results":[186],"can":[187],"obtained":[189],"Positive":[194],"kit.":[195],"SIGNIFICANCE":[196],"IMPACT":[198],"OF":[199],"THE":[200],"STUDY:":[201],"protocol":[204],"identified":[205],"herein":[206],"combined":[207],"is":[210],"novel":[211],"allows":[216],"levels":[220],"contaminated":[226]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}