{"id":"https://openalex.org/W1997957359","doi":"https://doi.org/10.1158/1535-7163.targ-09-b84","title":"Abstract B84: Withaferin-A is a novel HSF-1 inhibitor with potent antitumor effects in melanoma in vivo","display_name":"Abstract B84: Withaferin-A is a novel HSF-1 inhibitor with potent antitumor effects in melanoma in vivo","publication_year":2009,"publication_date":"2009-12-01","ids":{"openalex":"https://openalex.org/W1997957359","doi":"https://doi.org/10.1158/1535-7163.targ-09-b84","mag":"1997957359"},"language":"en","primary_location":{"id":"doi:10.1158/1535-7163.targ-09-b84","doi":"https://doi.org/10.1158/1535-7163.targ-09-b84","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1535-7163.targ-09-b84","pdf_url":null,"source":{"id":"https://openalex.org/S48437117","display_name":"Molecular Cancer Therapeutics","issn_l":"1535-7163","issn":["1535-7163","1538-8514"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Cancer Therapeutics","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111884806","display_name":"Abbas K. Samadi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Abbas K. Samadi","raw_affiliation_strings":["1 University of Kansas Medical Center, Kansas City, KS;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 University of Kansas Medical Center, Kansas City, KS;","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000215441","display_name":"Ridhwi Mukerji","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ridhwi Mukerji","raw_affiliation_strings":["1 University of Kansas Medical Center, Kansas City, KS;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 University of Kansas Medical Center, Kansas City, KS;","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102091332","display_name":"Anuj Shah","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anuj Shah","raw_affiliation_strings":["1 University of Kansas Medical Center, Kansas City, KS;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 University of Kansas Medical Center, Kansas City, KS;","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046504456","display_name":"Tyler Bron","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tyler Bron","raw_affiliation_strings":["1 University of Kansas Medical Center, Kansas City, KS;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 University of Kansas Medical Center, Kansas City, KS;","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111986568","display_name":"Barbara N. Timmermann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146416000","display_name":"University of Kansas","ror":"https://ror.org/001tmjg57","country_code":"US","type":"education","lineage":["https://openalex.org/I146416000"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Barbara N. Timmermann","raw_affiliation_strings":["2 University of Kansas, Lawrence, KS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2 University of Kansas, Lawrence, KS","institution_ids":["https://openalex.org/I146416000"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5057675169","display_name":"Mark Steven Cohen","orcid":"https://orcid.org/0000-0002-2517-0757","observed_orcids":["https://orcid.org/0000-0002-2517-0757"]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark S. Cohen","raw_affiliation_strings":["1 University of Kansas Medical Center, Kansas City, KS;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 University of Kansas Medical Center, Kansas City, KS;","institution_ids":["https://openalex.org/I4210128618"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"8","issue":"12_Supplement","first_page":"B84","last_page":"B84"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12298","display_name":"Phytochemicals and Medicinal Plants","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2707","display_name":"Complementary and alternative medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12298","display_name":"Phytochemicals and Medicinal Plants","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/2707","display_name":"Complementary and alternative medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11533","display_name":"Melanoma and MAPK Pathways","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/withaferin-a","display_name":"withaferin A","score":0.9419999718666077},{"id":"https://openalex.org/keywords/melanoma","display_name":"melanoma","score":0.9309999942779541},{"id":"https://openalex.org/keywords/heat-shock-proteins","display_name":"heat shock proteins","score":0.6370000243186951},{"id":"https://openalex.org/keywords/hsp72","display_name":"HSP72","score":0.9760000109672546},{"id":"https://openalex.org/keywords/mapk-signaling","display_name":"MAPK signaling","score":0.8100000023841858},{"id":"https://openalex.org/keywords/erk1-2","display_name":"ERK1/2","score":0.8429999947547913},{"id":"https://openalex.org/keywords/p38-mapk","display_name":"p38 MAPK","score":0.8849999904632568},{"id":"https://openalex.org/keywords/jnk","display_name":"JNK","score":0.8820000290870667},{"id":"https://openalex.org/keywords/akt","display_name":"Akt","score":0.5490000247955322},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.8379999995231628},{"id":"https://openalex.org/keywords/g2-m-cell-cycle-arrest","display_name":"G2/M cell cycle arrest","score":0.9330000281333923},{"id":"https://openalex.org/keywords/anticancer-activity","display_name":"anticancer activity","score":0.722000002861023},{"id":"https://openalex.org/keywords/metastatic-melanoma","display_name":"metastatic melanoma","score":0.8180000185966492},{"id":"https://openalex.org/keywords/orthotopic-murine-model","display_name":"orthotopic murine model","score":0.6759999990463257},{"id":"https://openalex.org/keywords/withanolides","display_name":"withanolides","score":0.75}],"concepts":[{"id":"https://openalex.org/C2776598214","wikidata":"https://www.wikidata.org/wiki/Q6606395","display_name":"Withaferin A","level":4,"score":0.9114507436752319},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.5835788249969482},{"id":"https://openalex.org/C2777658100","wikidata":"https://www.wikidata.org/wiki/Q180614","display_name":"Melanoma","level":2,"score":0.5645143985748291},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.5241339802742004},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.5215275883674622},{"id":"https://openalex.org/C88634738","wikidata":"https://www.wikidata.org/wiki/Q24769543","display_name":"Annexin","level":3,"score":0.5007519721984863},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4631803333759308},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.4624047875404358},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.4610063433647156},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.42384153604507446},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4204683005809784},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3781033754348755},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37573176622390747},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.33674097061157227},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3050886392593384},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2890227437019348},{"id":"https://openalex.org/C2780069950","wikidata":"https://www.wikidata.org/wiki/Q852660","display_name":"Withania somnifera","level":3,"score":0.12177535891532898},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11022523045539856},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.10674241185188293},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1535-7163.targ-09-b84","doi":"https://doi.org/10.1158/1535-7163.targ-09-b84","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1535-7163.targ-09-b84","pdf_url":null,"source":{"id":"https://openalex.org/S48437117","display_name":"Molecular Cancer Therapeutics","issn_l":"1535-7163","issn":["1535-7163","1538-8514"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Cancer Therapeutics","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.98580002784729}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2050114998","https://openalex.org/W3105597995","https://openalex.org/W2071912398","https://openalex.org/W2052966137","https://openalex.org/W1935845774","https://openalex.org/W2077080951","https://openalex.org/W2133897064","https://openalex.org/W1975217664","https://openalex.org/W3031952902","https://openalex.org/W3019271724"],"abstract_inverted_index":{"Abstract":[0],"Introduction:":[1],"Withaferin":[2,211,299],"A":[3,35,89,212,300,382,447,490],"(WA)":[4],"is":[5,448,475],"a":[6,45,239,294,346,449],"natural":[7],"product":[8],"withanolide":[9],"compound":[10],"with":[11,106,163,177,350,392,399],"historical":[12],"anti-inflammatory":[13],"activity":[14,18],"and":[15,40,60,76,98,109,115,132,147,173,186,193,204,218,266,284,296,312,338,372,417,457,467,471],"recent":[16],"anticancer":[17],"in":[19,36,44,69,194,221,249,277,288,293,314,328,335,345,369,394,427,455,458,484],"vitro.":[20],"The":[21],"purpose":[22],"of":[23,31,33,49,87,129,149,215,228,307,325,333,341,365,396,415,430,462,469,479,481,497],"this":[24],"study":[25],"was":[26,80,123,156,176,188,206,367,375,412,438],"to":[27,82,138,171,246,377,404,502],"evaluate":[28],"the":[29,61,84,140,370,378,428,436,476],"mechanism":[30],"action":[32],"withaferin":[34,88,362,381,446,489],"melanoma":[37,54,91,167,223,251,278,316,453,506],"cancer":[38,485],"cells":[39,55,252,454,486],"test":[41],"its":[42],"efficacy":[43],"murine":[46,62],"orthotopic":[47],"model":[48],"metastatic":[50,419],"melanoma.":[51],"Methods:":[52],"Human":[53],"lines":[56,225,253,291],"SKMEL28,":[57],"NPA,":[58],"DRO":[59],"B16F10":[63,166],"cell":[64,73,77,95,216,219,224,240,290,317,330,463],"line":[65],"were":[66,100,136,197],"used":[67,137],"for":[68,181,208,500,505],"vitro":[70,192,456],"analysis.":[71,113,127],"MTS":[72],"viability":[74,217],"assay":[75],"proliferation":[78,220],"analysis":[79,155,321,359],"performed":[81,157],"determine":[83],"concentration-dependent":[85],"effect":[86],"on":[90,94,158,259,269],"cells.":[92,279],"Effects":[93],"cycle":[96,241,464],"arrest":[97,245,248],"apoptosis":[99,276],"evaluated":[101,124,199],"by":[102,125,421,488],"PI/Annexin":[103],"V":[104,256],"staining":[105,258],"flow":[107],"cytometry":[108],"confirmed":[110],"through":[111,460],"Western":[112,126,270],"MAPkinase":[114,144,311,470],"heat":[116,150],"shock":[117,151],"protein":[118,145],"expression":[119,146,320,327,364,483],"after":[120,423],"WA":[121,179,237,274,280,402],"treatment":[122,426,437],"Inhibitors":[128],"MEK1/2,":[130],"JNK":[131],"p38":[133,310],"MAP":[134,302],"kinase":[135,303],"examine":[139],"relationship":[141],"between":[142],"WA-mediated":[143],"modulation":[148,468],"proteins.":[152],"In":[153,191,384],"vivo":[154,195,385,459],"BalbC":[159],"mice":[160],"injected":[161],"s.c.":[162],"5":[164,400],"million":[165],"cells,":[168,337],"allowing":[169],"tumors":[170],"grow":[172],"metastasize.":[174],"Treatment":[175],"5mg/kg/d":[178],"i.p.":[180],"3":[182,424],"weeks.":[183],"Tumor":[184],"growth":[185],"survival":[187],"followed":[189],"prospectively.":[190],"variables":[196],"statistically":[198],"using":[200],"SPSS":[201],"17.0":[202],"software":[203],"significance":[205],"defined":[207],"p<0.05.":[209],"Results:":[210],"induces":[213,275],"reduction":[214,332,340,353],"all":[222,250,289,315,329],"tested":[226,292],"[Range":[227],"IC50(B16F10":[229],"=209.7\u00b116nM);":[230],"(SKMEL-28=650.1":[231],"\u00b125nM)].":[232],"Mechanistic":[233],"studies":[234,271,386,499],"demonstrate":[235,387],"that":[236,273,361,373,445],"induced":[238,487],"shift":[242],"from":[243],"G0/G1":[244],"G2/M":[247],"tested.":[254],"Annexin":[255],"/PI":[257],"FC":[260],"as":[261,263],"well":[262],"caspase":[264],"activation":[265,306],"PARP":[267],"cleavage":[268],"confirm":[272],"reduced":[281],"p-Akt":[282],"levels":[283,287,344],"total":[285],"Akt":[286],"concentration":[295],"time-dependent":[297],"manner.":[298],"modulates":[301],"proteins,":[304],"inducing":[305],"p-ERK1/2":[308],",":[309],"SAPK/JNK":[313],"lines.":[318],"HSP":[319,472],"yielded":[322],"strong":[323,339],"induction":[324,461],"HSP72":[326,366,374],"lines,":[331],"HSP90":[334],"SKMEL28":[336],"HSF1":[342,482],"cellular":[343],"dose":[347],"dependant":[348],"manner":[349],"almost":[351,388],"complete":[352,389,413],"at":[354,408],"IC50":[355],"concentrations.":[356],"Confocal":[357],"microscopy":[358],"showed":[360],"A-induced":[363],"increased":[368],"cytoplasm":[371],"transported":[376],"nucleus":[379],"upon":[380],"stimulation.":[383],"tumor":[390],"regression":[391],"CR":[393],"80%":[395,429],"animals":[397,431],"treated":[398],"mg/kg/day":[401],"compared":[403],"controls":[405],"(p<0.01).":[406],"Additionally":[407],"5/mg/kg":[409],"dosing,":[410],"there":[411],"reversal":[414],"organ":[416],"lymphatic":[418],"disease":[420],"inspection":[422],"weeks":[425],"responding":[432],"without":[433],"progression":[434],"once":[435],"discontinued.":[439],"Conclusion:":[440],"Our":[441],"present":[442],"results":[443,493],"show":[444],"potent":[450],"therapy":[451],"against":[452],"arrest,":[465],"apoptosis,":[466],"expression.":[473],"This":[474],"first":[477],"report":[478],"inhibition":[480],"treatment.":[491],"These":[492],"support":[494],"future":[495],"proof":[496],"concept":[498],"translation":[501],"potential":[503],"therapies":[504],"patients.":[507],"Citation":[508],"Information:":[509],"Mol":[510],"Cancer":[511],"Ther":[512],"2009;8(12":[513],"Suppl):B84.":[514]},"counts_by_year":[{"year":2023,"cited_by_count":1}],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2016-06-24T00:00:00"}