{"id":"https://openalex.org/W1997656140","doi":"https://doi.org/10.1038/srep00213","title":"A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs","display_name":"A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs","publication_year":2012,"publication_date":"2012-01-06","ids":{"openalex":"https://openalex.org/W1997656140","doi":"https://doi.org/10.1038/srep00213","mag":"1997656140","pmid":"https://pubmed.ncbi.nlm.nih.gov/22355727"},"language":"en","primary_location":{"id":"doi:10.1038/srep00213","doi":"https://doi.org/10.1038/srep00213","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep00213","pdf_url":"https://www.nature.com/articles/srep00213.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/srep00213.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070964738","display_name":"Bahram Bob Valamehr","orcid":"https://orcid.org/0000-0002-5917-3585","observed_orcids":["https://orcid.org/0000-0002-5917-3585"]},"institutions":[{"id":"https://openalex.org/I4210155835","display_name":"Fate Therapeutics (United States)","ror":"https://ror.org/04p6jwd33","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155835"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bahram Valamehr","raw_affiliation_strings":["Fate Therapeutics Inc. , San Diego, CA,  USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fate Therapeutics Inc. , San Diego, CA,  USA","institution_ids":["https://openalex.org/I4210155835"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057388168","display_name":"Ramzey Abujarour","orcid":"https://orcid.org/0000-0002-7849-9441","observed_orcids":["https://orcid.org/0000-0002-7849-9441"]},"institutions":[{"id":"https://openalex.org/I4210155835","display_name":"Fate Therapeutics (United States)","ror":"https://ror.org/04p6jwd33","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155835"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ramzey Abujarour","raw_affiliation_strings":["Fate Therapeutics Inc., San Diego, CA, USA","Fate Therapeutics Inc., San Diego, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, CA, USA","institution_ids":["https://openalex.org/I4210155835"]},{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, USA","institution_ids":["https://openalex.org/I4210155835"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101686349","display_name":"Megan Robinson","orcid":"https://orcid.org/0000-0001-7626-3606","observed_orcids":["https://orcid.org/0000-0001-7626-3606"]},"institutions":[{"id":"https://openalex.org/I4210155835","display_name":"Fate Therapeutics (United States)","ror":"https://ror.org/04p6jwd33","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155835"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Megan Robinson","raw_affiliation_strings":["Fate Therapeutics Inc., San Diego, CA, USA","Fate Therapeutics Inc., San Diego, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, CA, USA","institution_ids":["https://openalex.org/I4210155835"]},{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, USA","institution_ids":["https://openalex.org/I4210155835"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113554814","display_name":"Thuy My Le","orcid":"https://orcid.org/0009-0008-7827-9060","observed_orcids":["https://orcid.org/0009-0008-7827-9060"]},"institutions":[{"id":"https://openalex.org/I4210155835","display_name":"Fate Therapeutics (United States)","ror":"https://ror.org/04p6jwd33","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155835"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thuy Le","raw_affiliation_strings":["Fate Therapeutics Inc., San Diego, CA, USA","Fate Therapeutics Inc., San Diego, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, CA, USA","institution_ids":["https://openalex.org/I4210155835"]},{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, USA","institution_ids":["https://openalex.org/I4210155835"]}]},{"author_position":"middle","author":{"id":null,"display_name":"David Robbins","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210155835","display_name":"Fate Therapeutics (United States)","ror":"https://ror.org/04p6jwd33","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155835"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David Robbins","raw_affiliation_strings":["Fate Therapeutics Inc., San Diego, CA, USA","Fate Therapeutics Inc., San Diego, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, CA, USA","institution_ids":["https://openalex.org/I4210155835"]},{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, USA","institution_ids":["https://openalex.org/I4210155835"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109439004","display_name":"Daniel D. Shoemaker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210155835","display_name":"Fate Therapeutics (United States)","ror":"https://ror.org/04p6jwd33","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155835"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel Shoemaker","raw_affiliation_strings":["Fate Therapeutics Inc., San Diego, CA, USA","Fate Therapeutics Inc., San Diego, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, CA, USA","institution_ids":["https://openalex.org/I4210155835"]},{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, USA","institution_ids":["https://openalex.org/I4210155835"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077712761","display_name":"Peter Flynn","orcid":"https://orcid.org/0009-0008-7704-3718","observed_orcids":["https://orcid.org/0009-0008-7704-3718"]},"institutions":[{"id":"https://openalex.org/I4210155835","display_name":"Fate Therapeutics (United States)","ror":"https://ror.org/04p6jwd33","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155835"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter Flynn","raw_affiliation_strings":["Fate Therapeutics Inc., San Diego, CA, USA","Fate Therapeutics Inc., San Diego, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, CA, USA","institution_ids":["https://openalex.org/I4210155835"]},{"raw_affiliation_string":"Fate Therapeutics Inc., San Diego, USA","institution_ids":["https://openalex.org/I4210155835"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210155835"],"apc_list":{"value":2690,"currency":"USD","value_usd":2690},"apc_paid":{"value":2690,"currency":"USD","value_usd":2690},"fwci":2.6806,"has_fulltext":true,"cited_by_count":77,"citation_normalized_percentile":{"value":0.90725302,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"2","issue":"1","first_page":"213","last_page":"213"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-induced-pluripotent-stem-cells","display_name":"human induced pluripotent stem cells","score":0.9829999804496765},{"id":"https://openalex.org/keywords/feeder-free-culture","display_name":"feeder-free culture","score":0.9940000176429749},{"id":"https://openalex.org/keywords/genomic-stability","display_name":"genome stability","score":0.7459999918937683},{"id":"https://openalex.org/keywords/trilineage-differentiation","display_name":"trilineage differentiation","score":0.847000002861023},{"id":"https://openalex.org/keywords/karyotype","display_name":"karyotype","score":0.7549999952316284},{"id":"https://openalex.org/keywords/copy-number-variation","display_name":"copy number variation","score":0.8840000033378601}],"concepts":[{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.6750888824462891},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.6582098007202148},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.5823734402656555},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5562498569488525},{"id":"https://openalex.org/C111696304","wikidata":"https://www.wikidata.org/wiki/Q2303697","display_name":"Sorting","level":2,"score":0.5235975980758667},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5233718156814575},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.449716180562973},{"id":"https://openalex.org/C10854531","wikidata":"https://www.wikidata.org/wiki/Q1061415","display_name":"Regenerative medicine","level":3,"score":0.4335799813270569},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3764176070690155},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3014146685600281},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.2821206748485565},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.27047914266586304},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.21924328804016113},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.21141237020492554},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10664677619934082},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1038/srep00213","doi":"https://doi.org/10.1038/srep00213","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep00213","pdf_url":"https://www.nature.com/articles/srep00213.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmid:22355727","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22355727","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3252544","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3252544","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sci Rep","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1038/srep00213","doi":"https://doi.org/10.1038/srep00213","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep00213","pdf_url":"https://www.nature.com/articles/srep00213.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.46810001134872437,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"score":0.5400000214576721,"display_name":"Industry, innovation and infrastructure","id":"https://metadata.un.org/sdg/9"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1997656140.pdf","grobid_xml":"https://content.openalex.org/works/W1997656140.grobid-xml"},"referenced_works_count":50,"referenced_works":["https://openalex.org/W1975716459","https://openalex.org/W1978173381","https://openalex.org/W1986371962","https://openalex.org/W1992663780","https://openalex.org/W2002163959","https://openalex.org/W2011177881","https://openalex.org/W2013205740","https://openalex.org/W2014416902","https://openalex.org/W2019229253","https://openalex.org/W2027833338","https://openalex.org/W2036632122","https://openalex.org/W2037338370","https://openalex.org/W2049888495","https://openalex.org/W2054916576","https://openalex.org/W2058421656","https://openalex.org/W2058521801","https://openalex.org/W2058981318","https://openalex.org/W2059378220","https://openalex.org/W2062049325","https://openalex.org/W2076194888","https://openalex.org/W2086663462","https://openalex.org/W2086851461","https://openalex.org/W2086943348","https://openalex.org/W2089426706","https://openalex.org/W2092215311","https://openalex.org/W2095668414","https://openalex.org/W2098597036","https://openalex.org/W2099914822","https://openalex.org/W2100417606","https://openalex.org/W2101736621","https://openalex.org/W2103540918","https://openalex.org/W2105361655","https://openalex.org/W2111888351","https://openalex.org/W2112485709","https://openalex.org/W2125691342","https://openalex.org/W2125987139","https://openalex.org/W2126432370","https://openalex.org/W2138033616","https://openalex.org/W2138977668","https://openalex.org/W2140201853","https://openalex.org/W2142046859","https://openalex.org/W2147547310","https://openalex.org/W2150197150","https://openalex.org/W2151220680","https://openalex.org/W2152426454","https://openalex.org/W2152483372","https://openalex.org/W2157713892","https://openalex.org/W2158011874","https://openalex.org/W2158979552","https://openalex.org/W2163404991"],"related_works":["https://openalex.org/W2126478593","https://openalex.org/W1480032740","https://openalex.org/W2117576492","https://openalex.org/W2189154201","https://openalex.org/W1996948977","https://openalex.org/W2071569010","https://openalex.org/W2008535483","https://openalex.org/W2343590058","https://openalex.org/W4229649984","https://openalex.org/W3039556430"],"abstract_inverted_index":{"Human":[0],"induced":[1],"pluripotent":[2],"stem":[3],"cells":[4],"(hiPSCs)":[5],"hold":[6],"enormous":[7],"potential,":[8],"however":[9],"several":[10],"obstacles":[11],"impede":[12],"their":[13,99],"translation":[14],"to":[15,25],"industrial":[16,134],"and":[17,29,33,57,76,82,101,110,126,131,135],"clinical":[18,136],"applications.":[19],"Here":[20],"we":[21,50],"describe":[22],"a":[23,41,70,118],"platform":[24,60,120],"efficiently":[26],"generate,":[27],"characterize":[28],"maintain":[30],"single":[31],"cell":[32],"feeder-free":[34],"(FF)":[35],"cultured":[36],"hiPSCs":[37],"by":[38,106],"means":[39],"of":[40,72,128,138],"small":[42],"molecule":[43],"cocktail":[44],"media":[45],"additive.":[46],"Using":[47],"this":[48],"strategy":[49],"have":[51],"developed":[52],"an":[53],"effective":[54],"multiplex":[55],"sorting":[56],"high-throughput":[58],"selection":[59],"where":[61],"individual":[62],"clonal":[63],"hiPSC":[64,86,129],"lines":[65],"are":[66],"readily":[67],"obtained":[68],"from":[69],"pool":[71],"candidate":[73],"clones,":[74],"expanded":[75,91],"thoroughly":[77],"characterized.":[78],"By":[79],"promoting":[80],"survival":[81],"self-renewal,":[83],"the":[84,133],"selected":[85],"clones":[87],"can":[88],"be":[89],"rapidly":[90],"over":[92],"multiple":[93],"FF,":[94],"single-cell":[95],"passages":[96],"while":[97],"maintaining":[98],"pluripotency":[100],"genomic":[102],"stability":[103],"as":[104],"demonstrated":[105],"trilineage":[107],"differentiation,":[108],"karyotype":[109],"copy":[111],"number":[112],"variation":[113],"analysis.":[114],"This":[115],"study":[116],"provides":[117],"robust":[119],"that":[121],"increases":[122],"efficiency,":[123],"throughput,":[124],"scale":[125],"quality":[127],"generation":[130],"facilitates":[132],"use":[137],"iPSC":[139],"technology.":[140]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":10},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2025-10-10T00:00:00"}