{"id":"https://openalex.org/W1997650053","doi":"https://doi.org/10.1016/s0021-9673(00)94134-7","title":"High-performance liquid chromatography of proteins on n-methylpyridinium polymer columns","display_name":"High-performance liquid chromatography of proteins on n-methylpyridinium polymer columns","publication_year":1989,"publication_date":"1989-01-01","ids":{"openalex":"https://openalex.org/W1997650053","doi":"https://doi.org/10.1016/s0021-9673(00)94134-7","mag":"1997650053","pmid":"https://pubmed.ncbi.nlm.nih.gov/2777945"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9673(00)94134-7","doi":"https://doi.org/10.1016/s0021-9673(00)94134-7","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0021-9673(00)94134-7","pdf_url":null,"source":{"id":"https://openalex.org/S176136234","display_name":"Journal of Chromatography A","issn_l":"0021-9673","issn":["0021-9673","1873-3778"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chromatography A","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000828303","display_name":"Atsushi Sugii","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I96036126","display_name":"Kumamoto University","ror":"https://ror.org/02cgss904","country_code":"JP","type":"education","lineage":["https://openalex.org/I96036126"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Atsushi Sugii","raw_affiliation_strings":["Faculty of Pharmaceutical Sciences, Kumamoto University, Japan","Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Pharmaceutical Sciences, Kumamoto University, Japan","institution_ids":["https://openalex.org/I96036126"]},{"raw_affiliation_string":"Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan","institution_ids":["https://openalex.org/I96036126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110144753","display_name":"Kumiko Harada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I96036126","display_name":"Kumamoto University","ror":"https://ror.org/02cgss904","country_code":"JP","type":"education","lineage":["https://openalex.org/I96036126"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kumiko Harada","raw_affiliation_strings":["Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan","institution_ids":["https://openalex.org/I96036126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102735085","display_name":"Koichi Nishimura","orcid":"https://orcid.org/0000-0003-4995-1096","observed_orcids":["https://orcid.org/0000-0003-4995-1096","https://orcid.org/0000-0003-0651-7484"]},"institutions":[{"id":"https://openalex.org/I96036126","display_name":"Kumamoto University","ror":"https://ror.org/02cgss904","country_code":"JP","type":"education","lineage":["https://openalex.org/I96036126"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Koichi Nishimura","raw_affiliation_strings":["Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan","institution_ids":["https://openalex.org/I96036126"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Ryosuke Hanaoka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I96036126","display_name":"Kumamoto University","ror":"https://ror.org/02cgss904","country_code":"JP","type":"education","lineage":["https://openalex.org/I96036126"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ryosuke Hanaoka","raw_affiliation_strings":["Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan","institution_ids":["https://openalex.org/I96036126"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110620099","display_name":"Seiichi Masuda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I96036126","display_name":"Kumamoto University","ror":"https://ror.org/02cgss904","country_code":"JP","type":"education","lineage":["https://openalex.org/I96036126"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Seiichi Masuda","raw_affiliation_strings":["Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Pharmaceutical Sciences, Kumamoto University, 5\u20101 Oe\u2010honmachi, Kumamoto, 862, Japan","institution_ids":["https://openalex.org/I96036126"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I96036126"],"apc_list":{"value":4040,"currency":"USD","value_usd":4040},"apc_paid":null,"fwci":1.1104,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.70843759,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"472","issue":"2","first_page":"357","last_page":"364"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0478999987244606,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.00570000009611249,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-separation","display_name":"protein separation","score":0.8320000171661377},{"id":"https://openalex.org/keywords/ion-exchange-chromatography","display_name":"ion-exchange chromatography","score":0.7039999961853027},{"id":"https://openalex.org/keywords/divinylbenzene","display_name":"divinylbenzene","score":0.8560000061988831},{"id":"https://openalex.org/keywords/ethylene-glycol-dimethacrylate","display_name":"ethylene glycol dimethacrylate","score":0.9990000128746033},{"id":"https://openalex.org/keywords/polymer-networks","display_name":"polymer networks","score":0.7850000262260437},{"id":"https://openalex.org/keywords/protein-recovery","display_name":"protein recovery","score":0.5210000276565552}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9330760836601257},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.811896800994873},{"id":"https://openalex.org/C2777682263","wikidata":"https://www.wikidata.org/wiki/Q413085","display_name":"Divinylbenzene","level":5,"score":0.7377370595932007},{"id":"https://openalex.org/C81145429","wikidata":"https://www.wikidata.org/wiki/Q7783245","display_name":"Thermoresponsive polymers in chromatography","level":4,"score":0.6835795640945435},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.6004288196563721},{"id":"https://openalex.org/C2777516009","wikidata":"https://www.wikidata.org/wiki/Q194207","display_name":"Ethylene glycol","level":2,"score":0.5218685865402222},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.514917254447937},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.5148845911026001},{"id":"https://openalex.org/C206967267","wikidata":"https://www.wikidata.org/wiki/Q905749","display_name":"Ion chromatography","level":2,"score":0.4987156391143799},{"id":"https://openalex.org/C116817701","wikidata":"https://www.wikidata.org/wiki/Q5955221","display_name":"Hydrophilic interaction chromatography","level":3,"score":0.48701196908950806},{"id":"https://openalex.org/C117760992","wikidata":"https://www.wikidata.org/wiki/Q389137","display_name":"Ion exchange","level":3,"score":0.487004816532135},{"id":"https://openalex.org/C51724486","wikidata":"https://www.wikidata.org/wiki/Q908955","display_name":"Column chromatography","level":2,"score":0.42092013359069824},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.17690297961235046},{"id":"https://openalex.org/C145148216","wikidata":"https://www.wikidata.org/wiki/Q36496","display_name":"Ion","level":2,"score":0.17211124300956726},{"id":"https://openalex.org/C15920480","wikidata":"https://www.wikidata.org/wiki/Q421281","display_name":"Copolymer","level":3,"score":0.12364527583122253},{"id":"https://openalex.org/C2777566558","wikidata":"https://www.wikidata.org/wiki/Q28917","display_name":"Styrene","level":4,"score":0.108725905418396},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D011726","descriptor_name":"Pyridinium Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013056","descriptor_name":"Spectrophotometry, Ultraviolet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9673(00)94134-7","doi":"https://doi.org/10.1016/s0021-9673(00)94134-7","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0021-9673(00)94134-7","pdf_url":null,"source":{"id":"https://openalex.org/S176136234","display_name":"Journal of Chromatography A","issn_l":"0021-9673","issn":["0021-9673","1873-3778"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chromatography A","raw_type":"journal-article"},{"id":"pmid:2777945","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2777945","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of chromatography","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W1964289386","https://openalex.org/W1965604856","https://openalex.org/W1970775870","https://openalex.org/W1983357075","https://openalex.org/W1986934284","https://openalex.org/W2002072246","https://openalex.org/W2002526239","https://openalex.org/W2012631889","https://openalex.org/W2030469267","https://openalex.org/W2040812785","https://openalex.org/W2043401648","https://openalex.org/W2046628637","https://openalex.org/W2064843998","https://openalex.org/W2065327235","https://openalex.org/W2072657800","https://openalex.org/W2081699927"],"related_works":["https://openalex.org/W2074968488","https://openalex.org/W2413569163","https://openalex.org/W2081699927","https://openalex.org/W1972403483","https://openalex.org/W2045832949","https://openalex.org/W2060921475","https://openalex.org/W2094077256","https://openalex.org/W2033137804","https://openalex.org/W2922194823","https://openalex.org/W2538526604"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}