{"id":"https://openalex.org/W1997634470","doi":"https://doi.org/10.1002/elps.1150150173","title":"Phosphopeptide mapping and phosphoamino acid analysis by electrophoresis and chromatography on thin\u2010layer cellulose plates","display_name":"Phosphopeptide mapping and phosphoamino acid analysis by electrophoresis and chromatography on thin\u2010layer cellulose plates","publication_year":1994,"publication_date":"1994-01-01","ids":{"openalex":"https://openalex.org/W1997634470","doi":"https://doi.org/10.1002/elps.1150150173","mag":"1997634470","pmid":"https://pubmed.ncbi.nlm.nih.gov/8055882"},"language":"en","primary_location":{"id":"doi:10.1002/elps.1150150173","doi":"https://doi.org/10.1002/elps.1150150173","is_oa":false,"landing_page_url":"https://doi.org/10.1002/elps.1150150173","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050932509","display_name":"Peter van der Geer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1307098950","display_name":"Salk Institute for Biological Studies","ror":"https://ror.org/03xez1567","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1307098950"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter van der Geer","raw_affiliation_strings":["Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037","Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037","institution_ids":["https://openalex.org/I1307098950"]},{"raw_affiliation_string":"Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA","institution_ids":["https://openalex.org/I1307098950"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5010612497","display_name":"Tony R. Hunter","orcid":"https://orcid.org/0000-0002-7691-6993","observed_orcids":["https://orcid.org/0000-0002-7691-6993"]},"institutions":[{"id":"https://openalex.org/I1307098950","display_name":"Salk Institute for Biological Studies","ror":"https://ror.org/03xez1567","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1307098950"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Tony Hunter","raw_affiliation_strings":["Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA","institution_ids":["https://openalex.org/I1307098950"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5010612497"],"corresponding_institution_ids":["https://openalex.org/I1307098950"],"apc_list":{"value":3940,"currency":"USD","value_usd":3940},"apc_paid":null,"fwci":2.5465,"has_fulltext":false,"cited_by_count":136,"citation_normalized_percentile":{"value":0.88983394,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"15","issue":"1","first_page":"544","last_page":"554"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phosphopeptide-mapping","display_name":"phosphopeptide mapping","score":0.8870000243186951},{"id":"https://openalex.org/keywords/phosphorylation-sites","display_name":"phosphorylation sites","score":0.9229999780654907},{"id":"https://openalex.org/keywords/phosphoamino-acid-analysis","display_name":"phosphoamino acid analysis","score":0.9639999866485596},{"id":"https://openalex.org/keywords/partial-acid-hydrolysis","display_name":"partial acid hydrolysis","score":0.9010000228881836},{"id":"https://openalex.org/keywords/edman-degradation","display_name":"Edman degradation","score":0.8349999785423279},{"id":"https://openalex.org/keywords/immunoprecipitation","display_name":"immunoprecipitation","score":0.847000002861023},{"id":"https://openalex.org/keywords/phosphoproteins","display_name":"phosphoproteins","score":0.7789999842643738}],"concepts":[{"id":"https://openalex.org/C2779933727","wikidata":"https://www.wikidata.org/wiki/Q7187535","display_name":"Phosphopeptide","level":3,"score":0.9158868193626404},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7593135833740234},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5869446396827698},{"id":"https://openalex.org/C2779251873","wikidata":"https://www.wikidata.org/wiki/Q80294","display_name":"Cellulose","level":2,"score":0.541481077671051},{"id":"https://openalex.org/C190196608","wikidata":"https://www.wikidata.org/wiki/Q746133","display_name":"Thin-layer chromatography","level":2,"score":0.5032636523246765},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.4809819459915161},{"id":"https://openalex.org/C3019891514","wikidata":"https://www.wikidata.org/wiki/Q7784286","display_name":"Thin layer","level":3,"score":0.4260578155517578},{"id":"https://openalex.org/C2779227376","wikidata":"https://www.wikidata.org/wiki/Q6505497","display_name":"Layer (electronics)","level":2,"score":0.3828284740447998},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13533592224121094},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.07715362310409546},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.06152793765068054}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001345","descriptor_name":"Autoradiography","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002855","descriptor_name":"Chromatography, Thin Layer","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007202","descriptor_name":"Indicators and Reagents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010710","descriptor_name":"Phosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010748","descriptor_name":"Phosphopeptides","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D010748","descriptor_name":"Phosphopeptides","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D010748","descriptor_name":"Phosphopeptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010761","descriptor_name":"Phosphorus Radioisotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015180","descriptor_name":"Electrophoresis, Gel, Two-Dimensional","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/elps.1150150173","doi":"https://doi.org/10.1002/elps.1150150173","is_oa":false,"landing_page_url":"https://doi.org/10.1002/elps.1150150173","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},{"id":"pmid:8055882","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8055882","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W986536428","https://openalex.org/W1000436420","https://openalex.org/W1014698510","https://openalex.org/W1030517862","https://openalex.org/W1041041130","https://openalex.org/W1150077517","https://openalex.org/W1491832097","https://openalex.org/W1505363336","https://openalex.org/W1512678537","https://openalex.org/W1528493806","https://openalex.org/W1541726653","https://openalex.org/W1572940336","https://openalex.org/W1576340647","https://openalex.org/W1605839892","https://openalex.org/W1608621302","https://openalex.org/W1679522460","https://openalex.org/W1864043375","https://openalex.org/W1908892780","https://openalex.org/W1968013329","https://openalex.org/W1992628345","https://openalex.org/W2004686233","https://openalex.org/W2005529016","https://openalex.org/W2011147958","https://openalex.org/W2012514869","https://openalex.org/W2016189963","https://openalex.org/W2030577752","https://openalex.org/W2032359283","https://openalex.org/W2033599095","https://openalex.org/W2034239247","https://openalex.org/W2037434894","https://openalex.org/W2048863622","https://openalex.org/W2048875557","https://openalex.org/W2053799953","https://openalex.org/W2057799558","https://openalex.org/W2067226707","https://openalex.org/W2073478078","https://openalex.org/W2084877581","https://openalex.org/W2091848990","https://openalex.org/W2139248684","https://openalex.org/W2160845560","https://openalex.org/W2182061241","https://openalex.org/W2185377255","https://openalex.org/W2275843281","https://openalex.org/W2398661146","https://openalex.org/W2412384539","https://openalex.org/W2471026972","https://openalex.org/W2471078498"],"related_works":["https://openalex.org/W2290182149","https://openalex.org/W2917699684","https://openalex.org/W1528493806","https://openalex.org/W2123485598","https://openalex.org/W2465378152","https://openalex.org/W380698346","https://openalex.org/W2496611847","https://openalex.org/W2059286336","https://openalex.org/W2421230118","https://openalex.org/W2013748646"],"abstract_inverted_index":{"Identification":[0],"of":[1,13,19,36,42,50,95,100,146,176,183,190],"protein":[2,22],"phosphorylation":[3,37],"sites":[4,15,38],"is":[5,72,115],"essential":[6],"in":[7,54,63,111,133,170],"order":[8],"to":[9,16,83,117,179],"evaluate":[10],"the":[11,17,34,51,64,93,96,113,122,144,173,177,181,187],"contribution":[12],"individual":[14,105],"regulation":[18],"a":[20,28,77,184,191],"particular":[21],"by":[23,68,131,149,155,160],"phosphorylation.":[24],"Here":[25],"we":[26,30,139],"review":[27],"method":[29,71],"have":[31],"developed":[32],"for":[33,92,142],"identification":[35],"based":[39],"on":[40,57,127],"digestion":[41,52,151],"32P-labeled":[43],"proteins":[44],"with":[45,152,172],"site-specific":[46,153],"proteases":[47],"and":[48,104,121,159,162],"separation":[49],"products":[53,124],"two":[55,136],"dimensions":[56],"thin-layer":[58,128],"cellulose":[59,129],"plates":[60,130],"using":[61],"electrophoresis":[62,132],"first":[65],"dimension":[66],"followed":[67],"chromatography.":[69],"This":[70],"very":[73],"sensitive,":[74],"requiring":[75],"only":[76],"few":[78],"hundred":[79],"32P-disintegrations":[80],"per":[81],"minute":[82],"obtain":[84],"reproducible":[85],"phosphopeptide":[86,185],"maps.":[87],"We":[88],"also":[89],"report":[90],"methods":[91,141],"analysis":[94],"phosphoamino":[97],"acid":[98,119],"content":[99],"both":[101],"intact":[102],"phosphoproteins":[103],"phosphopeptides":[106,148],"recovered":[107],"from":[108,186],"two-dimensional":[109,174],"separations,":[110],"which":[112],"material":[114],"subjected":[116],"partial":[118],"hydrolysis,":[120],"hydrolysis":[123],"are":[125],"separated":[126],"one":[134],"or":[135],"dimensions.":[137],"Finally,":[138],"describe":[140],"analyzing":[143],"structure":[145],"isolated":[147],"secondary":[150],"proteases,":[154],"manual":[156],"Edman":[157],"degradation,":[158],"immunoprecipitation,":[161],"indicate":[163],"how":[164],"this":[165],"information":[166],"can":[167],"be":[168],"used":[169],"conjunction":[171],"mobility":[175],"peptide":[178],"deduce":[180],"identity":[182],"known":[188],"sequence":[189],"protein.":[192]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}