{"id":"https://openalex.org/W1997218780","doi":null,"title":"A modified procedure for quantitative analysis of mtDNA, detecting mtDNA depletion.","display_name":"A modified procedure for quantitative analysis of mtDNA, detecting mtDNA depletion.","publication_year":2005,"publication_date":"2005-01-01","ids":{"openalex":"https://openalex.org/W1997218780","mag":"1997218780","pmid":"https://pubmed.ncbi.nlm.nih.gov/16278519"},"language":"en","primary_location":{"id":"pmid:16278519","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16278519","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of applied genetics","raw_type":"Comparative Study"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088741041","display_name":"Anna Weglewska","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40413290","display_name":"University of Gda\u0144sk","ror":"https://ror.org/011dv8m48","country_code":"PL","type":"education","lineage":["https://openalex.org/I40413290"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Anna Weglewska","raw_affiliation_strings":["Department of Molecular Biology, University of Gda\u0144sk, Kladki 24, 80-822 Gda\u0144sk, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology, University of Gda\u0144sk, Kladki 24, 80-822 Gda\u0144sk, Poland","institution_ids":["https://openalex.org/I40413290"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063125955","display_name":"Joanna Jak\u00f3bkiewicz\u2010Banecka","orcid":"https://orcid.org/0000-0002-8846-5448","observed_orcids":["https://orcid.org/0000-0002-8846-5448"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joanna Jak\u00f3bkiewicz-Banecka","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5060642903","display_name":"Grzegorz W\u0119grzyn","orcid":"https://orcid.org/0000-0003-4042-7466","observed_orcids":["https://orcid.org/0000-0003-4042-7466","https://orcid.org/0009-0007-5525-9430"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Grzegorz Wegrzyn","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1034,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.36954346,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"46","issue":"4","first_page":"423","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11027","display_name":"Metabolism and Genetic Disorders","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mitochondrial-dna","display_name":"mitochondrial DNA","score":0.8489999771118164},{"id":"https://openalex.org/keywords/mtdna-depletion","display_name":"mtDNA depletion","score":0.8199999928474426},{"id":"https://openalex.org/keywords/nucleic-acid-hybridization","display_name":"nucleic acid hybridization","score":0.44200000166893005},{"id":"https://openalex.org/keywords/densitometric-analysis","display_name":"densitometric analysis","score":0.8840000033378601},{"id":"https://openalex.org/keywords/mitochondrial-disease","display_name":"mitochondrial disease","score":0.5630000233650208},{"id":"https://openalex.org/keywords/tissue-samples","display_name":"tissue samples","score":0.36500000953674316}],"concepts":[{"id":"https://openalex.org/C24586158","wikidata":"https://www.wikidata.org/wiki/Q27075","display_name":"Mitochondrial DNA","level":3,"score":0.9293907284736633},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8918766975402832},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6401441097259521},{"id":"https://openalex.org/C95986675","wikidata":"https://www.wikidata.org/wiki/Q185168","display_name":"Quantitative analysis (chemistry)","level":2,"score":0.5460706353187561},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4971058666706085},{"id":"https://openalex.org/C145169222","wikidata":"https://www.wikidata.org/wiki/Q9339485","display_name":"Molecular probe","level":3,"score":0.4920472800731659},{"id":"https://openalex.org/C130197536","wikidata":"https://www.wikidata.org/wiki/Q418436","display_name":"Hybridization probe","level":3,"score":0.4912204146385193},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.47824564576148987},{"id":"https://openalex.org/C19811304","wikidata":"https://www.wikidata.org/wiki/Q6930378","display_name":"mtDNA control region","level":5,"score":0.46315306425094604},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.4443667531013489},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.44157400727272034},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.4228418469429016},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3741312623023987},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1771608293056488},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.09408581256866455},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.08435377478599548},{"id":"https://openalex.org/C197754878","wikidata":"https://www.wikidata.org/wiki/Q80711","display_name":"Haplotype","level":4,"score":0.054282426834106445},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D004272","descriptor_name":"DNA, Mitochondrial","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D004272","descriptor_name":"DNA, Mitochondrial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004587","descriptor_name":"Electrophoresis, Agar Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D015336","descriptor_name":"Molecular Probe Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:16278519","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16278519","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of applied genetics","raw_type":"Comparative Study"},{"id":"mag:1997218780","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16278519","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W170813420","https://openalex.org/W1576093526","https://openalex.org/W1967683528","https://openalex.org/W1989697190","https://openalex.org/W1993259508","https://openalex.org/W1994623311","https://openalex.org/W1994993378","https://openalex.org/W2018590086","https://openalex.org/W2020210226","https://openalex.org/W2021407576","https://openalex.org/W2028997150","https://openalex.org/W2033170821","https://openalex.org/W2038697027","https://openalex.org/W2038948948","https://openalex.org/W2062681082","https://openalex.org/W2068663070","https://openalex.org/W2132584299","https://openalex.org/W2140354171","https://openalex.org/W2145370285","https://openalex.org/W2157133565"],"related_works":["https://openalex.org/W2326890519","https://openalex.org/W1852699976","https://openalex.org/W7097355662","https://openalex.org/W2061843271","https://openalex.org/W2060283226","https://openalex.org/W2110486743","https://openalex.org/W2136408833","https://openalex.org/W2090590443","https://openalex.org/W2407392452","https://openalex.org/W177678514"],"abstract_inverted_index":{"Quantitative":[0],"analysis":[1,153],"of":[2,11,27,79,85,124,137,140,156,177,201],"mitochondrial":[3],"DNA":[4,65,103,161,179],"(mtDNA)":[5],"is":[6,29,58,109,147,171],"crucial":[7],"for":[8,51,111,163],"proper":[9],"diagnosis":[10],"diseases":[12,197],"that":[13,168],"are":[14,121],"caused":[15,198],"by":[16,199],"or":[17,105],"associated":[18],"with":[19,118,127],"mtDNA":[20,28,54,69,98,187],"depletion.":[21],"However,":[22],"such":[23],"a":[24,31,48,174],"quantitative":[25,52,172],"characterization":[26],"not":[30],"simple":[32],"procedure":[33,50,57,170],"and":[34,72,134],"requires":[35],"several":[36],"laboratory":[37],"steps":[38],"at":[39],"which":[40,146],"potential":[41],"errors":[42],"can":[43,88],"accumulate.":[44],"Here,":[45],"we":[46,165,182],"describe":[47],"modified":[49],"human":[53],"analysis.":[55],"The":[56],"based":[59],"on":[60],"using":[61],"two":[62],"PCR-amplified,":[63],"fluorescein-labeled":[64],"probes,":[66,145],"complementary":[67],"to":[68,93,185],"(detection":[70],"probe)":[71,120],"chromosomal":[73],"18S":[74,106],"rDNA":[75,107],"(reference":[76],"probe),":[77],"both":[78,128,144,157],"similar":[80],"length.":[81],"Thus,":[82],"equal":[83],"amounts":[84,162],"these":[86],"probes":[87],"be":[89],"used":[90],"and,":[91],"contrary":[92],"previously":[94],"published":[95],"procedures,":[96],"no":[97],"purification":[99],"(apart":[100],"from":[101,143,193,196],"total":[102],"isolation)":[104],"cloning":[108],"necessary":[110],"probe":[112],"preparation.":[113],"Two":[114],"separate":[115],"hybridizations":[116],"(each":[117],"one":[119,125],"suggested":[122],"instead":[123],"hybridization":[126],"probes;":[129],"this":[130],"decreases":[131],"background":[132],"signals":[133,142],"enables":[135],"adjustment":[136],"the":[138,150,169],"strength":[139],"specific":[141],"useful":[148],"in":[149,173,190],"subsequent":[151],"densitometric":[152],"after":[154],"superimposing":[155],"pictures.":[158],"Using":[159],"different":[160],"reactions,":[164],"have":[166],"proved":[167],"broad":[175],"range":[176],"sample":[178],"concentrations.":[180],"Moreover,":[181],"were":[183],"able":[184],"detect":[186],"depletion":[188],"unambiguously":[189],"tissue":[191],"samples":[192],"patients":[194],"suffering":[195],"dysfunction":[200],"mtDNA.":[202]},"counts_by_year":[{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}