{"id":"https://openalex.org/W199670788","doi":null,"title":"High expression of heterologous proteins by Saccharomyces cerevisiae grown on ethanol","display_name":"High expression of heterologous proteins by Saccharomyces cerevisiae grown on ethanol","publication_year":2006,"publication_date":"2006-09-18","ids":{"openalex":"https://openalex.org/W199670788","mag":"199670788"},"language":"en","primary_location":{"id":"pmh:oai:dspace.library.uu.nl:1874/12687","doi":null,"is_oa":true,"landing_page_url":"https://dspace.library.uu.nl/handle/1874/12687","pdf_url":null,"source":{"id":"https://openalex.org/S4306401649","display_name":"Utrecht University Repository (Utrecht University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I193662353","host_organization_name":"Utrecht University","host_organization_lineage":["https://openalex.org/I193662353"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://dspace.library.uu.nl/handle/1874/12687","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5069224844","display_name":"Antonius Martinus Johannes van de Laar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Antonius Martinus Johannes van de Laar","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5069224844"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.00419999985024333,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/recombinant-protein-secretion","display_name":"recombinant protein secretion","score":0.7919999957084656},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.9430000185966492},{"id":"https://openalex.org/keywords/glucose-derepression","display_name":"glucose derepression","score":0.7009999752044678},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.593999981880188},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.7940000295639038},{"id":"https://openalex.org/keywords/redox-stress","display_name":"redox stress","score":0.7540000081062317},{"id":"https://openalex.org/keywords/central-carbon-metabolism","display_name":"central carbon metabolism","score":0.7319999933242798},{"id":"https://openalex.org/keywords/post-golgi-transport","display_name":"post-Golgi transport","score":0.7870000004768372},{"id":"https://openalex.org/keywords/gene-expression-microarrays","display_name":"gene expression microarrays","score":0.6349999904632568},{"id":"https://openalex.org/keywords/pulse-chase","display_name":"pulse-chase","score":0.621999979019165},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.4659999907016754}],"concepts":[{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.7486931681632996},{"id":"https://openalex.org/C65183824","wikidata":"https://www.wikidata.org/wiki/Q194361","display_name":"Ethanol fuel","level":3,"score":0.7250196933746338},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.6671088933944702},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.6569207310676575},{"id":"https://openalex.org/C2780161600","wikidata":"https://www.wikidata.org/wiki/Q153","display_name":"Ethanol","level":2,"score":0.6503182649612427},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.6245269775390625},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5766539573669434},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5301616787910461},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.4023831784725189},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3222366273403168},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.32212167978286743},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23842966556549072},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08456966280937195}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:dspace.library.uu.nl:1874/12687","doi":null,"is_oa":true,"landing_page_url":"https://dspace.library.uu.nl/handle/1874/12687","pdf_url":null,"source":{"id":"https://openalex.org/S4306401649","display_name":"Utrecht University Repository (Utrecht University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I193662353","host_organization_name":"Utrecht University","host_organization_lineage":["https://openalex.org/I193662353"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},{"id":"pmh:uu:oai:dspace.library.uu.nl:1874/12687","doi":null,"is_oa":true,"landing_page_url":"https://dspace.library.uu.nl/handle/1874/12687","pdf_url":null,"source":{"id":"https://openalex.org/S4306401649","display_name":"Utrecht University Repository (Utrecht University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I193662353","host_organization_name":"Utrecht University","host_organization_lineage":["https://openalex.org/I193662353"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralthesis"},{"id":"mag:199670788","doi":null,"is_oa":false,"landing_page_url":"https://dspace.library.uu.nl/bitstream/1874/12687/12/index.htm","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:dspace.library.uu.nl:1874/12687","doi":null,"is_oa":true,"landing_page_url":"https://dspace.library.uu.nl/handle/1874/12687","pdf_url":null,"source":{"id":"https://openalex.org/S4306401649","display_name":"Utrecht University Repository (Utrecht University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I193662353","host_organization_name":"Utrecht University","host_organization_lineage":["https://openalex.org/I193662353"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":160,"referenced_works":["https://openalex.org/W49866477","https://openalex.org/W93457247","https://openalex.org/W180762096","https://openalex.org/W1507531440","https://openalex.org/W1516116810","https://openalex.org/W1534083934","https://openalex.org/W1555578917","https://openalex.org/W1565986670","https://openalex.org/W1570599752","https://openalex.org/W1572652548","https://openalex.org/W1574136604","https://openalex.org/W1590529516","https://openalex.org/W1604959358","https://openalex.org/W1755414476","https://openalex.org/W1789882194","https://openalex.org/W1790958488","https://openalex.org/W1821377433","https://openalex.org/W1846878385","https://openalex.org/W1879503128","https://openalex.org/W1903635354","https://openalex.org/W1964649535","https://openalex.org/W1967569948","https://openalex.org/W1968016929","https://openalex.org/W1969195165","https://openalex.org/W1970424709","https://openalex.org/W1972582931","https://openalex.org/W1972584860","https://openalex.org/W1975471112","https://openalex.org/W1977933239","https://openalex.org/W1978794333","https://openalex.org/W1979008608","https://openalex.org/W1979924833","https://openalex.org/W1981385746","https://openalex.org/W1981648996","https://openalex.org/W1983078722","https://openalex.org/W1987087859","https://openalex.org/W1993107991","https://openalex.org/W1994817218","https://openalex.org/W1996276569","https://openalex.org/W2002029926","https://openalex.org/W2002635370","https://openalex.org/W2006338742","https://openalex.org/W2009953715","https://openalex.org/W2010250072","https://openalex.org/W2012775161","https://openalex.org/W2014258732","https://openalex.org/W2015031589","https://openalex.org/W2017172632","https://openalex.org/W2018008917","https://openalex.org/W2018325461","https://openalex.org/W2018611506","https://openalex.org/W2020559016","https://openalex.org/W2020853874","https://openalex.org/W2023747754","https://openalex.org/W2024244058","https://openalex.org/W2025826330","https://openalex.org/W2029112137","https://openalex.org/W2029766795","https://openalex.org/W2030209236","https://openalex.org/W2031189086","https://openalex.org/W2034194918","https://openalex.org/W2034309871","https://openalex.org/W2034687123","https://openalex.org/W2038605297","https://openalex.org/W2038708830","https://openalex.org/W2046871655","https://openalex.org/W2047798770","https://openalex.org/W2047920199","https://openalex.org/W2051496342","https://openalex.org/W2052559147","https://openalex.org/W2052976072","https://openalex.org/W2054172648","https://openalex.org/W2056376534","https://openalex.org/W2057865094","https://openalex.org/W2058268622","https://openalex.org/W2061058657","https://openalex.org/W2061469583","https://openalex.org/W2063289321","https://openalex.org/W2066060904","https://openalex.org/W2068176739","https://openalex.org/W2069522776","https://openalex.org/W2071046609","https://openalex.org/W2072106875","https://openalex.org/W2072402939","https://openalex.org/W2073414410","https://openalex.org/W2076687620","https://openalex.org/W2078174502","https://openalex.org/W2078383484","https://openalex.org/W2079280181","https://openalex.org/W2082573660","https://openalex.org/W2087754611","https://openalex.org/W2088933296","https://openalex.org/W2092205827","https://openalex.org/W2092846883","https://openalex.org/W2093160301","https://openalex.org/W2093728186","https://openalex.org/W2095818547","https://openalex.org/W2095830750","https://openalex.org/W2097202353","https://openalex.org/W2097957919","https://openalex.org/W2098189454","https://openalex.org/W2101420820","https://openalex.org/W2103081714","https://openalex.org/W2103857608","https://openalex.org/W2104775769","https://openalex.org/W2106161171","https://openalex.org/W2106928991","https://openalex.org/W2107451370","https://openalex.org/W2107601804","https://openalex.org/W2108940138","https://openalex.org/W2113310577","https://openalex.org/W2113827842","https://openalex.org/W2114721511","https://openalex.org/W2114874722","https://openalex.org/W2115124797","https://openalex.org/W2115687906","https://openalex.org/W2118120822","https://openalex.org/W2118260141","https://openalex.org/W2119475405","https://openalex.org/W2123380574","https://openalex.org/W2124144946","https://openalex.org/W2127917585","https://openalex.org/W2128220257","https://openalex.org/W2129887584","https://openalex.org/W2131989143","https://openalex.org/W2132990040","https://openalex.org/W2135335146","https://openalex.org/W2135752997","https://openalex.org/W2135998024","https://openalex.org/W2137076128","https://openalex.org/W2137662702","https://openalex.org/W2137683543","https://openalex.org/W2137755719","https://openalex.org/W2139178532","https://openalex.org/W2140670503","https://openalex.org/W2141588510","https://openalex.org/W2141590023","https://openalex.org/W2143062330","https://openalex.org/W2145855403","https://openalex.org/W2145944929","https://openalex.org/W2147333853","https://openalex.org/W2147799840","https://openalex.org/W2148361216","https://openalex.org/W2149503684","https://openalex.org/W2149797789","https://openalex.org/W2150637735","https://openalex.org/W2153283265","https://openalex.org/W2156398887","https://openalex.org/W2157163471","https://openalex.org/W2158735148","https://openalex.org/W2159294156","https://openalex.org/W2163334233","https://openalex.org/W2165011536","https://openalex.org/W2165710053","https://openalex.org/W2167450976","https://openalex.org/W2169142563","https://openalex.org/W2169597956","https://openalex.org/W2169875902","https://openalex.org/W2170707503","https://openalex.org/W2171463471"],"related_works":["https://openalex.org/W2127917585","https://openalex.org/W89301068","https://openalex.org/W2023941558","https://openalex.org/W2002290892","https://openalex.org/W2046194476","https://openalex.org/W7096670507","https://openalex.org/W1985333430","https://openalex.org/W2005003991","https://openalex.org/W4323256409","https://openalex.org/W2041935559"],"abstract_inverted_index":{"The":[0,399],"production":[1,64,170,245,463],"of":[2,6,22,150,162,194,212,223,240,254,263,395,401,421,458,485],"recombinant":[3],"proteins":[4,185,404],"is":[5,118,386,405,448],"great":[7],"importance":[8],"for":[9,68,178,305],"industrial":[10,19],"applications":[11],"in":[12,33,98,112,141,167,190,201,203,225,270,375,389,414,451,460,464,478],"fields":[13],"such":[14],"as":[15,43,86,326,328,392],"pharmaceutical":[16],"ingredients":[17],"and":[18,58,75,143,155,243,260,285,312,318,357,362,384,417,430,443,474,483],"enzymes.":[20],"One":[21],"these":[23,264],"products":[24],"are":[25,187,198,331],"camelid":[26],"antibody":[27],"fragments,":[28],"produced":[29,189],"by":[30,71,146,234,298,350],"Saccharomyces":[31,465],"cerevisiae":[32,466],"high":[34],"cell":[35,230,337,472],"density":[36],"fed":[37,101,226],"batch":[38,102,142,227],"fermentation":[39,103],"processes,":[40],"using":[41,235,351],"glucose":[42,242,332,369],"sole":[44,87],"carbon":[45,88,276],"source.":[46],"To":[47,218,334],"improve":[48],"this":[49,116],"production,":[50,343],"process":[51,117,123,442],"optimisation":[52],"was":[53,66,79,96,124,173,232,267,316,347],"performed":[54],"on":[55,159,214,407,439,471],"both":[56],"biological":[57],"technical":[59],"aspects.":[60],"First":[61],"the":[62,93,113,129,148,160,163,204,221,229,241,252,274,302,336,344,352,376,381,396,426,440,444,456],"whole":[63],"system":[65],"analysed":[67,349],"possible":[69],"improvements":[70],"comparing":[72],"shake":[73],"flask":[74],"fed-batch":[76,144,272],"cultivation.":[77],"It":[78,105,182,266],"found":[80,268,317],"that":[81,107,184,186,269,330,367],"not":[82],"glucose,":[83],"but":[84],"ethanol":[85,100,130,156,205,213,224,244,271,390,452,459],"source":[89],"could":[90],"greatly":[91],"increase":[92,413],"productivity,":[94],"which":[95,172,476],"verified":[97],"an":[99,168,372,412],"protocol.":[104],"showed":[106,366],"especially":[108],"under":[109],"limiting":[110],"conditions":[111],"final":[114],"phase,":[115],"much":[119,293],"more":[120],"efficient.":[121],"This":[122,165,207,365],"further":[125,348],"developed":[126],"to":[127,132,176,250,280,290,301,339,411,434],"exploit":[128],"characteristics":[131,137,197],"its":[133],"fullest":[134],"potential.":[135],"These":[136],"were":[138,248,322],"first":[139],"determined":[140],"experiments":[145,361],"investigating":[147],"influence":[149,211,253],"growth":[151,258],"rate,":[152],"oxygen":[153],"concentration":[154],"accumulation":[157],"level":[158],"productivity":[161],"process.":[164,206,246],"resulted":[166],"improved":[169,200],"protocol":[171],"also":[174],"shown":[175],"work":[177],"other":[179],"heterologous":[180,341,377,461,486],"proteins.":[181,424,487],"appeared":[183],"normally":[188],"small":[191],"quantities":[192],"because":[193],"their":[195],"structural":[196],"significantly":[199],"secretion":[202,345,481],"suggested":[208],"a":[209,256,261,292,308,393,418,436,468,479],"strong":[210,310,469],"protein":[215,319,342,462],"folding":[216,415,441],"processes.":[217],"better":[219],"understand":[220],"impact":[222,438,470],"fermentations,":[228],"state":[231,338,473],"investigated":[233],"DNA":[236,353],"micro":[237,354],"array":[238],"analysis":[239],"Samples":[247],"taken":[249],"verify":[251],"ethanol,":[255,408],"decreasing":[257],"rate":[259],"combination":[262],"two.":[265],"fermentations":[273],"central":[275,303],"metabolism":[277,288,304,475],"behaves":[278],"according":[279],"earlier":[281],"descriptions.":[282],"But":[283],"nitrogen":[284],"fatty":[286],"acid":[287],"seem":[289,433],"play":[291],"bigger":[294],"role":[295,374],"than":[296],"expected":[297],"directing":[299],"intermediates":[300],"replenishment.":[306],"Also":[307],"very":[309],"redox":[311,428],"oxidative":[313,431],"stress":[314,432],"effect":[315],"catabolic":[320],"processes":[321],"highly":[323],"up":[324],"regulated":[325,388],"well":[327],"genes":[329],"repressed.":[333],"link":[335],"increased":[340],"pathway":[346],"arrays":[355],"data":[356],"performing":[358],"pulse":[359],"chase":[360],"electron":[363],"microscopy.":[364],"indeed":[368],"derepression":[370],"plays":[371],"important":[373],"gene":[378],"transcription.":[379],"However,":[380],"general":[382],"transcription":[383],"translation":[385],"down":[387],"cultivation":[391],"result":[394],"changed":[397,427],"environment.":[398],"flux":[400,484],"correctly":[402],"folded":[403,423],"higher":[406,419,480],"probably":[409],"due":[410],"capacity":[416,482],"turnover":[420],"incorrect":[422],"Furthermore,":[425],"potential":[429],"have":[435],"large":[437],"post":[445],"Golgi":[446],"transport":[447],"strongly":[449],"induced":[450],"cultivated":[453],"cells.":[454],"Therefore,":[455],"use":[457],"has":[467],"results":[477]},"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}
