{"id":"https://openalex.org/W1996646258","doi":"https://doi.org/10.1046/j.1440-169x.2002.00636.x","title":"A novel Dictyostelium Cdk8 is required for aggregation, but is dispensable for growth","display_name":"A novel Dictyostelium Cdk8 is required for aggregation, but is dispensable for growth","publication_year":2002,"publication_date":"2002-06-01","ids":{"openalex":"https://openalex.org/W1996646258","doi":"https://doi.org/10.1046/j.1440-169x.2002.00636.x","mag":"1996646258","pmid":"https://pubmed.ncbi.nlm.nih.gov/12060071"},"language":"en","primary_location":{"id":"doi:10.1046/j.1440-169x.2002.00636.x","doi":"https://doi.org/10.1046/j.1440-169x.2002.00636.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1440-169x.2002.00636.x","pdf_url":null,"source":{"id":"https://openalex.org/S103234284","display_name":"Development Growth & Differentiation","issn_l":"0012-1592","issn":["0012-1592","1440-169X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Development, Growth & Differentiation","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5047499058","display_name":"Kosuke Takeda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kosuke Takeda","raw_affiliation_strings":["Division of Biological Sciences, Graduate School of Science, Hokkaido University Sapporo, Japan","Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060\u20130810, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences, Graduate School of Science, Hokkaido University Sapporo, Japan","institution_ids":["https://openalex.org/I205349734"]},{"raw_affiliation_string":"Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060\u20130810, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078428660","display_name":"Tamao Saito","orcid":"https://orcid.org/0000-0003-0368-4244","observed_orcids":["https://orcid.org/0000-0003-0368-4244"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tamao Saito","raw_affiliation_strings":["Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060\u20130810, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060\u20130810, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047004566","display_name":"Hiroshi Ochiai","orcid":"https://orcid.org/0000-0002-2200-1325","observed_orcids":["https://orcid.org/0000-0002-2200-1325"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hiroshi Ochiai","raw_affiliation_strings":["Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060\u20130810, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060\u20130810, Japan","institution_ids":["https://openalex.org/I205349734"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5047004566"],"corresponding_institution_ids":["https://openalex.org/I205349734"],"apc_list":{"value":3990,"currency":"USD","value_usd":3990},"apc_paid":null,"fwci":1.3585,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.79161438,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"44","issue":"3","first_page":"213","last_page":"223"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13362","display_name":"Slime Mold and Myxomycetes Research","score":0.8651999831199646,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13362","display_name":"Slime Mold and Myxomycetes Research","score":0.8651999831199646,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.10740000009536743,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12789","display_name":"Planarian Biology and Electrostimulation","score":0.003700000001117587,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dictyostelium","display_name":"Dictyostelium","score":0.9769999980926514},{"id":"https://openalex.org/keywords/cdk8","display_name":"CDK8","score":0.9039999842643738},{"id":"https://openalex.org/keywords/cell-aggregation","display_name":"cell aggregation","score":0.8410000205039978},{"id":"https://openalex.org/keywords/starvation","display_name":"starvation","score":0.6650000214576721},{"id":"https://openalex.org/keywords/fruiting-body-development","display_name":"fruiting body formation","score":0.6859999895095825},{"id":"https://openalex.org/keywords/genetic-complementation","display_name":"gene complementation","score":0.6819999814033508},{"id":"https://openalex.org/keywords/epistasis","display_name":"epistasis","score":0.8709999918937683},{"id":"https://openalex.org/keywords/cdc2-related-kinase","display_name":"cdc2-related kinase","score":0.7400000095367432},{"id":"https://openalex.org/keywords/growth-rate","display_name":"growth rate","score":0.4189999997615814},{"id":"https://openalex.org/keywords/insertional-mutagenesis","display_name":"insertional mutagenesis","score":0.7390000224113464}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7747498750686646},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6286647915840149},{"id":"https://openalex.org/C2775889303","wikidata":"https://www.wikidata.org/wiki/Q17146891","display_name":"Dictyostelium","level":3,"score":0.593940019607544},{"id":"https://openalex.org/C115867849","wikidata":"https://www.wikidata.org/wiki/Q753175","display_name":"Protein-fragment complementation assay","level":5,"score":0.5374278426170349},{"id":"https://openalex.org/C120504264","wikidata":"https://www.wikidata.org/wiki/Q1147372","display_name":"Cyclin-dependent kinase 1","level":4,"score":0.5237200856208801},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5043653249740601},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.49144160747528076},{"id":"https://openalex.org/C107635520","wikidata":"https://www.wikidata.org/wiki/Q1967554","display_name":"Insertional mutagenesis","level":4,"score":0.4494771361351013},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4493674039840698},{"id":"https://openalex.org/C188082640","wikidata":"https://www.wikidata.org/wiki/Q1780899","display_name":"Complementation","level":4,"score":0.41435351967811584},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.17304351925849915}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004023","descriptor_name":"Dictyostelium","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D004023","descriptor_name":"Dictyostelium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004023","descriptor_name":"Dictyostelium","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018844","descriptor_name":"Cyclin-Dependent Kinases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018844","descriptor_name":"Cyclin-Dependent Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018844","descriptor_name":"Cyclin-Dependent Kinases","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1440-169x.2002.00636.x","doi":"https://doi.org/10.1046/j.1440-169x.2002.00636.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1440-169x.2002.00636.x","pdf_url":null,"source":{"id":"https://openalex.org/S103234284","display_name":"Development Growth & Differentiation","issn_l":"0012-1592","issn":["0012-1592","1440-169X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Development, Growth & Differentiation","raw_type":"journal-article"},{"id":"pmid:12060071","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12060071","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Development, growth & differentiation","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W19919172","https://openalex.org/W94487769","https://openalex.org/W305314246","https://openalex.org/W619953506","https://openalex.org/W1664256523","https://openalex.org/W1966222088","https://openalex.org/W1975435021","https://openalex.org/W1982307936","https://openalex.org/W2001369616","https://openalex.org/W2001406822","https://openalex.org/W2002806722","https://openalex.org/W2006126837","https://openalex.org/W2010604041","https://openalex.org/W2026715418","https://openalex.org/W2039942900","https://openalex.org/W2044713924","https://openalex.org/W2046235933","https://openalex.org/W2055621332","https://openalex.org/W2058851268","https://openalex.org/W2061317184","https://openalex.org/W2061597992","https://openalex.org/W2062145837","https://openalex.org/W2063056851","https://openalex.org/W2064033758","https://openalex.org/W2069959093","https://openalex.org/W2080176881","https://openalex.org/W2084607632","https://openalex.org/W2088903986","https://openalex.org/W2090690907","https://openalex.org/W2096699962","https://openalex.org/W2097706568","https://openalex.org/W2130821820","https://openalex.org/W2135563174","https://openalex.org/W2151513534","https://openalex.org/W2156480647","https://openalex.org/W2163914666","https://openalex.org/W2169295539","https://openalex.org/W2173713067","https://openalex.org/W2185975859","https://openalex.org/W2291822757","https://openalex.org/W2314225431","https://openalex.org/W4295216797"],"related_works":["https://openalex.org/W1983806781","https://openalex.org/W2007716206","https://openalex.org/W2066797208","https://openalex.org/W2115358164","https://openalex.org/W7095801955","https://openalex.org/W2036250566","https://openalex.org/W2001206945","https://openalex.org/W1983057915","https://openalex.org/W3020854855","https://openalex.org/W1161620077"],"abstract_inverted_index":{"When":[0],"Dictyostelium":[1],"cells":[2,86,97,124,144,185],"starve,":[3],"they":[4,154],"express":[5,188],"genes":[6],"necessary":[7],"for":[8,179,193,201],"aggregation.":[9,180],"Using":[10],"insertional":[11],"mutagenesis,":[12],"we":[13,78,195],"have":[14],"isolated":[15],"a":[16,42,151],"mutant":[17],"that":[18,25,57,172,197,205],"does":[19],"not":[20,88,136,187],"aggregate":[21,89,157],"upon":[22],"starvation":[23],"and":[24,90,140,164],"forms":[26],"small":[27],"plaques":[28],"on":[29,106,150],"bacterial":[30,107,152],"lawns,":[31],"thus":[32],"indicating":[33,204],"slow":[34],"growth.":[35],"Sequencing":[36],"of":[37,49,73,119,212],"the":[38,46,58,71,122,142,159,189,206],"mutated":[39],"locus":[40],"showed":[41],"strong":[43],"similarity":[44],"to":[45,65,70,148,156],"catalytic":[47],"domain":[48],"cdc2-related":[50],"kinase":[51,67],"genes.":[52,216],"Phylogenetic":[53],"analysis":[54],"further":[55],"indicated":[56],"amino":[59],"acid":[60],"sequence":[61,72],"was":[62,162],"more":[63,93],"close":[64],"cyclin-dependent":[66,75],"8":[68],"than":[69,95],"other":[74,215],"kinases.":[76],"Thus,":[77],"designated":[79],"this":[80,120],"gene":[81],"as":[82,158],"Ddcdk8.":[83],"The":[84,169],"Ddcdk8-null":[85,123,184],"do":[87,186],"grow":[91,149],"somewhat":[92],"slowly":[94],"parental":[96],"when":[98,141,182],"being":[99],"shaken":[100],"in":[101],"axenic":[102],"medium":[103],"or":[104],"laid":[105],"plates.":[108],"To":[109],"confirm":[110],"whether":[111],"these":[112],"defective":[113],"phenotypes":[114],"were":[115,125,145],"caused":[116],"by":[117],"disruption":[118],"gene,":[121],"complemented":[126,143],"with":[127],"DdCdk8":[128,175,207],"protein":[129],"expressed":[130],"from":[131],"an":[132,137],"endogenous":[133],"promoter,":[134,139],"but":[135],"actin":[138],"then":[146],"allowed":[147],"lawn,":[153],"began":[155],"food":[160],"supply":[161],"depleted":[163],"finally":[165],"became":[166],"fruiting":[167],"bodies.":[168],"results":[170],"suggest":[171],"properly":[173],"regulated":[174],"activity":[176],"is":[177,199],"essential":[178],"Because,":[181],"starved,":[183],"acaA":[190,202,213],"transcripts":[191],"required":[192],"aggregation,":[194],"deduce":[196],"Ddcdk8":[198],"epistatic":[200],"expression,":[203],"products":[208],"may":[209],"regulate":[210],"expression":[211],"and/or":[214]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2013,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}