{"id":"https://openalex.org/W1996541145","doi":"https://doi.org/10.1163/156854107781352034","title":"Identification of reference genes for qRT-PCR studies of gene expression in giant cells and syncytia induced in Arabidopsis thaliana by Meloidogyne incognita and Heterodera schachtii","display_name":"Identification of reference genes for qRT-PCR studies of gene expression in giant cells and syncytia induced in Arabidopsis thaliana by Meloidogyne incognita and Heterodera schachtii","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W1996541145","doi":"https://doi.org/10.1163/156854107781352034","mag":"1996541145"},"language":"en","primary_location":{"id":"doi:10.1163/156854107781352034","doi":"https://doi.org/10.1163/156854107781352034","is_oa":false,"landing_page_url":"https://doi.org/10.1163/156854107781352034","pdf_url":null,"source":{"id":"https://openalex.org/S2055470","display_name":"Nematology","issn_l":"1388-5545","issn":["1388-5545","1568-5411"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320561","host_organization_name":"Brill","host_organization_lineage":["https://openalex.org/P4310320561","https://openalex.org/P4310313990"],"host_organization_lineage_names":["Brill","De Gruyter"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nematology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110197357","display_name":"Julia P. Hofmann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I92869138","display_name":"BOKU University","ror":"https://ror.org/057ff4y42","country_code":"AT","type":"education","lineage":["https://openalex.org/I92869138"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Julia Hofmann","raw_affiliation_strings":["Department of Applied Plant Sciences and Plant Biotechnology, Institute of Plant Protection, University of Natural Resources and Applied Life Sciences, Vienna, Peter-Jordan Strasse 82, 1190 Vienna, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Applied Plant Sciences and Plant Biotechnology, Institute of Plant Protection, University of Natural Resources and Applied Life Sciences, Vienna, Peter-Jordan Strasse 82, 1190 Vienna, Austria","institution_ids":["https://openalex.org/I92869138"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088527081","display_name":"Florian M. W. Grundler","orcid":"https://orcid.org/0000-0001-8101-0558","observed_orcids":["https://orcid.org/0000-0001-8101-0558"]},"institutions":[{"id":"https://openalex.org/I92869138","display_name":"BOKU University","ror":"https://ror.org/057ff4y42","country_code":"AT","type":"education","lineage":["https://openalex.org/I92869138"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Florian M.W. Grundler","raw_affiliation_strings":["Department of Applied Plant Sciences and Plant Biotechnology, Institute of Plant Protection, University of Natural Resources and Applied Life Sciences, Vienna, Peter-Jordan Strasse 82, 1190 Vienna, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Applied Plant Sciences and Plant Biotechnology, Institute of Plant Protection, University of Natural Resources and Applied Life Sciences, Vienna, Peter-Jordan Strasse 82, 1190 Vienna, Austria","institution_ids":["https://openalex.org/I92869138"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I92869138"],"apc_list":null,"apc_paid":null,"fwci":3.2941,"has_fulltext":false,"cited_by_count":36,"citation_normalized_percentile":{"value":0.91074332,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"9","issue":"3","first_page":"317","last_page":"323"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11179","display_name":"Nematode management and characterization studies","score":0.9458000063896179,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11179","display_name":"Nematode management and characterization studies","score":0.9458000063896179,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.05130000039935112,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11074","display_name":"Helminth infection and control","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/reference-genes","display_name":"reference genes","score":0.9909999966621399},{"id":"https://openalex.org/keywords/rt-qpcr-normalization","display_name":"qRT-PCR normalization","score":0.8500000238418579},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.8389999866485596},{"id":"https://openalex.org/keywords/meloidogyne-incognita","display_name":"Meloidogyne incognita","score":0.9909999966621399},{"id":"https://openalex.org/keywords/heterodera-schachtii","display_name":"Heterodera schachtii","score":0.9929999709129333},{"id":"https://openalex.org/keywords/cyst-nematodes","display_name":"cyst nematodes","score":0.8190000057220459},{"id":"https://openalex.org/keywords/root-knot-nematode","display_name":"root-knot nematode","score":1.0},{"id":"https://openalex.org/keywords/plant-parasitic-nematodes","display_name":"plant-parasitic nematodes","score":0.8339999914169312},{"id":"https://openalex.org/keywords/giant-cells","display_name":"giant cells","score":0.7179999947547913},{"id":"https://openalex.org/keywords/syncytia","display_name":"syncytia","score":0.9860000014305115},{"id":"https://openalex.org/keywords/housekeeping-genes","display_name":"housekeeping genes","score":0.6909999847412109},{"id":"https://openalex.org/keywords/18s-rrna","display_name":"18S rRNA","score":0.996999979019165}],"concepts":[{"id":"https://openalex.org/C2780079514","wikidata":"https://www.wikidata.org/wiki/Q2203128","display_name":"Heterodera schachtii","level":3,"score":0.9332648515701294},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9150968790054321},{"id":"https://openalex.org/C33922130","wikidata":"https://www.wikidata.org/wiki/Q419232","display_name":"Housekeeping gene","level":4,"score":0.830451250076294},{"id":"https://openalex.org/C2780617777","wikidata":"https://www.wikidata.org/wiki/Q628493","display_name":"Meloidogyne incognita","level":3,"score":0.6972625851631165},{"id":"https://openalex.org/C2775988993","wikidata":"https://www.wikidata.org/wiki/Q158695","display_name":"Arabidopsis thaliana","level":4,"score":0.65108323097229},{"id":"https://openalex.org/C2779148672","wikidata":"https://www.wikidata.org/wiki/Q25326537","display_name":"Reference genes","level":4,"score":0.6370288133621216},{"id":"https://openalex.org/C2778830712","wikidata":"https://www.wikidata.org/wiki/Q5185","display_name":"Nematode","level":2,"score":0.5892841219902039},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.585429847240448},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5651827454566956},{"id":"https://openalex.org/C2781269127","wikidata":"https://www.wikidata.org/wiki/Q2072680","display_name":"Nematode infection","level":3,"score":0.5288613438606262},{"id":"https://openalex.org/C2776207046","wikidata":"https://www.wikidata.org/wiki/Q2215256","display_name":"Root-knot nematode","level":3,"score":0.4876631498336792},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4235798120498657},{"id":"https://openalex.org/C2778505058","wikidata":"https://www.wikidata.org/wiki/Q149049","display_name":"Pratylenchus penetrans","level":3,"score":0.42263540625572205},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.3853940963745117},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.15349379181861877},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1163/156854107781352034","doi":"https://doi.org/10.1163/156854107781352034","is_oa":false,"landing_page_url":"https://doi.org/10.1163/156854107781352034","pdf_url":null,"source":{"id":"https://openalex.org/S2055470","display_name":"Nematology","issn_l":"1388-5545","issn":["1388-5545","1568-5411"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320561","host_organization_name":"Brill","host_organization_lineage":["https://openalex.org/P4310320561","https://openalex.org/P4310313990"],"host_organization_lineage_names":["Brill","De Gruyter"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nematology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8409000039100647,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321181","display_name":"Austrian Science Fund","ror":"https://ror.org/013tf3c58"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2032392216","https://openalex.org/W4206891732","https://openalex.org/W4311510835","https://openalex.org/W7116393660","https://openalex.org/W3005396977","https://openalex.org/W2911823286","https://openalex.org/W2153171245","https://openalex.org/W2949848598","https://openalex.org/W2977728133","https://openalex.org/W2051445778"],"abstract_inverted_index":{"Abstract":[0],"Sedentary":[1],"plant-parasitic":[2],"nematodes,":[3,9],"such":[4],"as":[5,43,93],"cyst":[6,132],"and":[7,35,63,90,118,133],"root-knot":[8,134],"induce":[10],"feeding":[11],"structures":[12],"in":[13,21,80,103,107],"the":[14,22,53,81,113],"host":[15,60],"root":[16],"that":[17],"undergo":[18],"extensive":[19],"changes":[20],"gene":[23,32,101],"expression.":[24],"This":[25],"phenomenon":[26],"has":[27],"previously":[28],"been":[29],"studied":[30],"by":[31],"chip":[33],"analysis":[34],"qRT-PCR.":[36],"Housekeeping":[37],"genes":[38,68,129],"are":[39,126],"often":[40],"used":[41],"routinely":[42],"internal":[44],"references":[45],"for":[46,96,130],"relative":[47,97],"qRT-PCR":[48,77,98],"analyses.":[49,78],"However,":[50],"due":[51],"to":[52],"strong":[54],"influence":[55],"of":[56,66,76,100,109],"nematode":[57,104,135],"infection":[58,105],"on":[59],"cell":[61],"metabolism":[62],"physiology,":[64],"expression":[65,102],"housekeeping":[67],"may":[69],"be":[70],"altered":[71],"considerably,":[72],"thus":[73],"limiting":[74],"reliability":[75],"Therefore,":[79],"present":[82],"work":[83],"we":[84],"tested":[85,114],"UBQ10,":[86],"ACT2,":[87],"EF1a,":[88],"UBP22":[89,117],"18S":[91,119],"rRNA":[92,120],"potential":[94],"candidates":[95,115],"studies":[99],"sites":[106],"roots":[108],"Arabidopsis":[110],"thaliana.":[111],"Among":[112],"only":[116],"were":[121],"stably":[122],"expressed":[123],"and,":[124],"therefore,":[125],"reliable":[127],"reference":[128],"studying":[131],"infections.":[136]},"counts_by_year":[{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":5}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}