{"id":"https://openalex.org/W1996482684","doi":"https://doi.org/10.1002/1522-2683(200206)23:11<1591::aid-elps1591>3.0.co;2-#","title":"Proteome analysis of camptothecin\u2010treated cortical neurons using isotope\u2010coded affinity tags","display_name":"Proteome analysis of camptothecin\u2010treated cortical neurons using isotope\u2010coded affinity tags","publication_year":2002,"publication_date":"2002-06-01","ids":{"openalex":"https://openalex.org/W1996482684","doi":"https://doi.org/10.1002/1522-2683(200206)23:11<1591::aid-elps1591>3.0.co;2-#","mag":"1996482684","pmid":"https://pubmed.ncbi.nlm.nih.gov/12179976"},"language":"en","primary_location":{"id":"doi:10.1002/1522-2683(200206)23:11<1591::aid-elps1591>3.0.co;2-#","doi":"https://doi.org/10.1002/1522-2683(200206)23:11<1591::aid-elps1591>3.0.co;2-#","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1522-2683(200206)23:11<1591::aid-elps1591>3.0.co;2-#","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088688923","display_name":"Li\u2010Rong Yu","orcid":"https://orcid.org/0000-0001-6084-991X","observed_orcids":["https://orcid.org/0000-0001-6084-991X"]},"institutions":[{"id":"https://openalex.org/I142606810","display_name":"Pacific Northwest National Laboratory","ror":"https://ror.org/05h992307","country_code":"US","type":"facility","lineage":["https://openalex.org/I1325736334","https://openalex.org/I1330989302","https://openalex.org/I142606810","https://openalex.org/I39565521"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Li\u2010Rong Yu","raw_affiliation_strings":["Pacific Northwest National Laboratory, Richland, WA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest National Laboratory, Richland, WA, USA","institution_ids":["https://openalex.org/I142606810"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101616645","display_name":"Mark D. Johnson","orcid":"https://orcid.org/0000-0002-4010-2605","observed_orcids":["https://orcid.org/0000-0002-4010-2605"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark D. Johnson","raw_affiliation_strings":["Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080595928","display_name":"Thomas P. Conrads","orcid":"https://orcid.org/0000-0003-4742-3281","observed_orcids":["https://orcid.org/0000-0003-4742-3281"]},"institutions":[{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas P. Conrads","raw_affiliation_strings":["SAIC\u2010Frederick, National Cancer Institute at Frederick, Frederick, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"SAIC\u2010Frederick, National Cancer Institute at Frederick, Frederick, MD, USA","institution_ids":["https://openalex.org/I4210140884"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023982706","display_name":"Richard D. Smith","orcid":"https://orcid.org/0000-0002-2381-2349","observed_orcids":["https://orcid.org/0000-0002-2381-2349"]},"institutions":[{"id":"https://openalex.org/I142606810","display_name":"Pacific Northwest National Laboratory","ror":"https://ror.org/05h992307","country_code":"US","type":"facility","lineage":["https://openalex.org/I1325736334","https://openalex.org/I1330989302","https://openalex.org/I142606810","https://openalex.org/I39565521"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard D. Smith","raw_affiliation_strings":["Pacific Northwest National Laboratory, Richland, WA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest National Laboratory, Richland, WA, USA","institution_ids":["https://openalex.org/I142606810"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080721533","display_name":"Richard S. Morrison","orcid":"https://orcid.org/0000-0002-5115-446X","observed_orcids":["https://orcid.org/0000-0002-5115-446X"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard S. Morrison","raw_affiliation_strings":["Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019010663","display_name":"Timothy D. Veenstra","orcid":"https://orcid.org/0000-0003-2941-0225","observed_orcids":["https://orcid.org/0000-0003-2941-0225"]},"institutions":[{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Timothy D. Veenstra","raw_affiliation_strings":["SAIC\u2010Frederick, National Cancer Institute at Frederick, Frederick, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"SAIC\u2010Frederick, National Cancer Institute at Frederick, Frederick, MD, USA","institution_ids":["https://openalex.org/I4210140884"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5019010663"],"corresponding_institution_ids":["https://openalex.org/I4210140884"],"apc_list":{"value":3940,"currency":"USD","value_usd":3940},"apc_paid":null,"fwci":2.5022,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.90240144,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"23","issue":"11","first_page":"1591","last_page":"1598"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/camptothecin","display_name":"camptothecin","score":0.9940000176429749},{"id":"https://openalex.org/keywords/cortical-neurons","display_name":"cortical neurons","score":0.9660000205039978},{"id":"https://openalex.org/keywords/icat","display_name":"ICAT","score":0.8040000200271606},{"id":"https://openalex.org/keywords/proteomics","display_name":"proteomics","score":0.6309999823570251},{"id":"https://openalex.org/keywords/protein-expression-profiling","display_name":"protein expression profiling","score":0.8119999766349792},{"id":"https://openalex.org/keywords/glycolytic-enzymes","display_name":"glycolytic enzymes","score":0.8500000238418579},{"id":"https://openalex.org/keywords/tricarboxylic-acid-cycle","display_name":"TCA cycle","score":0.9459999799728394},{"id":"https://openalex.org/keywords/mouse-brain","display_name":"mouse brain","score":0.4860000014305115}],"concepts":[{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.8029322624206543},{"id":"https://openalex.org/C2779682216","wikidata":"https://www.wikidata.org/wiki/Q419964","display_name":"Camptothecin","level":2,"score":0.6834031343460083},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5137385129928589},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4698079228401184},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.35350319743156433},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.34604090452194214},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3142659068107605},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29358306527137756}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000972","descriptor_name":"Antineoplastic Agents, Phytogenic","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002166","descriptor_name":"Camptothecin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002540","descriptor_name":"Cerebral Cortex","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008810","descriptor_name":"Mice, Inbred C57BL","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009474","descriptor_name":"Neurons","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D009474","descriptor_name":"Neurons","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/1522-2683(200206)23:11<1591::aid-elps1591>3.0.co;2-#","doi":"https://doi.org/10.1002/1522-2683(200206)23:11<1591::aid-elps1591>3.0.co;2-#","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1522-2683(200206)23:11<1591::aid-elps1591>3.0.co;2-#","pdf_url":null,"source":{"id":"https://openalex.org/S2849398","display_name":"Electrophoresis","issn_l":"0173-0835","issn":["0173-0835","1522-2683"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ELECTROPHORESIS","raw_type":"journal-article"},{"id":"pmid:12179976","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12179976","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Electrophoresis","raw_type":"Journal Article"},{"id":"pmh:oai:digitalcommons.cedarville.edu:pharmaceutical_sciences_publications-1505","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/496","pdf_url":null,"source":{"id":"https://openalex.org/S4377196552","display_name":"DigitalCommons-Cedarville (Cedarville University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I169138485","host_organization_name":"Cedarville University","host_organization_lineage":["https://openalex.org/I169138485"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Pharmaceutical Sciences Faculty Publications","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1356092923","display_name":null,"funder_award_id":"N01CO12400","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G174812244","display_name":"DCTD Computer Support","funder_award_id":"n01co12400-79-0-22","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1549338235","https://openalex.org/W1597678601","https://openalex.org/W1931234589","https://openalex.org/W1968198326","https://openalex.org/W1975970168","https://openalex.org/W1982535455","https://openalex.org/W1985692894","https://openalex.org/W1989518640","https://openalex.org/W2003925684","https://openalex.org/W2007805022","https://openalex.org/W2011358056","https://openalex.org/W2014458197","https://openalex.org/W2029987169","https://openalex.org/W2038575808","https://openalex.org/W2040941311","https://openalex.org/W2047007872","https://openalex.org/W2090399837","https://openalex.org/W2097326528","https://openalex.org/W2097463865","https://openalex.org/W2111208057","https://openalex.org/W2148101811","https://openalex.org/W2150566147","https://openalex.org/W2151982496","https://openalex.org/W2170471837","https://openalex.org/W2170949754"],"related_works":["https://openalex.org/W2032210210","https://openalex.org/W2033561045","https://openalex.org/W2418884099","https://openalex.org/W2489274754","https://openalex.org/W2148810272","https://openalex.org/W7098391420","https://openalex.org/W2156294202","https://openalex.org/W1554970564","https://openalex.org/W2587369432","https://openalex.org/W2109581992"],"abstract_inverted_index":{"Isotope-coded":[0],"affinity":[1],"tags":[2],"(ICATs)":[3],"were":[4,32,48,65,98,160,177],"employed":[5],"to":[6,73,105],"identify":[7],"and":[8,16,27,37,52,121,136,147,172],"quantitate":[9,106],"changes":[10],"in":[11,170],"protein":[12],"expression":[13,139],"between":[14],"control":[15,24,146],"camptothecin-treated":[17,148],"mouse":[18,149],"cortical":[19,25,150],"neurons.":[20,151],"Proteins":[21],"extracted":[22],"from":[23,145,154],"neurons":[26],"those":[28],"treated":[29],"with":[30,34],"camptothecin":[31],"labeled":[33],"the":[35,42,53,95,107,111,138,167,173],"light":[36],"heavy":[38],"isotopic":[39],"versions":[40],"of":[41,94,110,116,128,140,158,166],"ICAT":[43],"reagents,":[44],"respectively.":[45],"ICAT-labeled":[46,112],"samples":[47],"combined,":[49],"proteolytically":[50],"digested,":[51],"derivatized":[54],"peptides":[55,62,113],"isolated":[56,64],"using":[57,100,125],"immobilized":[58],"avidin":[59],"chromatography.":[60],"The":[61],"thus":[63,133],"analyzed":[66],"by":[67],"reversed-phase":[68],"liquid":[69],"chromatography":[70],"coupled":[71],"directly":[72],"either":[74],"a":[75,82,92,162],"conventional":[76],"ion-trap":[77],"mass":[78,88],"spectrometer":[79,89],"(IT-MS)":[80],"or":[81],"Fourier":[83],"transform":[84],"ion":[85],"cyclotron":[86],"resonance":[87],"(FTICR).":[90],"While":[91,152],"majority":[93],"peptide":[96],"identifications":[97],"accomplished":[99],"IT-MS,":[101],"FTICR":[102],"was":[103],"used":[104],"relative":[108],"abundances":[109],"taking":[114],"advantage":[115],"its":[117],"high":[118],"resolution,":[119],"sensitivity,":[120],"duty":[122],"cycle.":[123],"By":[124],"this":[126],"combination":[127],"MS":[129],"technologies":[130],"we":[131],"have":[132],"far":[134],"identified":[135],"quantified":[137],"greater":[141],"than":[142],"125":[143],"proteins":[144,153,159],"most":[155],"functional":[156],"classes":[157],"identified,":[161],"particularly":[163],"large":[164],"percentage":[165],"enzymes":[168],"involved":[169],"glycolysis":[171],"tricarboxylic":[174],"acid":[175],"cycle":[176],"observed.":[178]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}