{"id":"https://openalex.org/W1996479753","doi":"https://doi.org/10.1089/thy.1995.5.55","title":"A Novel, Nonradioactive in Vivo Bioassay of Thyrotropin (TSH)","display_name":"A Novel, Nonradioactive in Vivo Bioassay of Thyrotropin (TSH)","publication_year":1995,"publication_date":"1995-02-01","ids":{"openalex":"https://openalex.org/W1996479753","doi":"https://doi.org/10.1089/thy.1995.5.55","mag":"1996479753","pmid":"https://pubmed.ncbi.nlm.nih.gov/7787435"},"language":"en","primary_location":{"id":"doi:10.1089/thy.1995.5.55","doi":"https://doi.org/10.1089/thy.1995.5.55","is_oa":false,"landing_page_url":"https://doi.org/10.1089/thy.1995.5.55","pdf_url":null,"source":{"id":"https://openalex.org/S27569427","display_name":"Thyroid","issn_l":"1050-7256","issn":["1050-7256","1557-9077"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thyroid","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032197760","display_name":"Joyce East-Palmer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"JOYCE EAST-PALMER","raw_affiliation_strings":["Molecular and Cellular Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular and Cellular Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031319152","display_name":"Mariusz W. Szkudlinski","orcid":"https://orcid.org/0000-0002-5656-2104","observed_orcids":["https://orcid.org/0000-0002-5656-2104"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"MARIUSZ W. SZKUDLINSKI","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"JOHN LEE","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"JOHN LEE","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032350044","display_name":"NAGESWARA RAO THOTAKURA","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"N. RAO THOTAKURA","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112067957","display_name":"Bruce D. Weintraub","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"BRUCE D. WEINTRAUB","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.3672,"has_fulltext":false,"cited_by_count":48,"citation_normalized_percentile":{"value":0.87867718,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"5","issue":"1","first_page":"55","last_page":"59"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10293","display_name":"Thyroid Disorders and Treatments","score":0.9771000146865845,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10293","display_name":"Thyroid Disorders and Treatments","score":0.9771000146865845,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.013500000350177288,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10832","display_name":"Pituitary Gland Disorders and Treatments","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thyroid-stimulating-hormone","display_name":"thyroid-stimulating hormone","score":0.9470000267028809},{"id":"https://openalex.org/keywords/non-radioactive-assay","display_name":"non-radioactive assay","score":0.7350000143051147},{"id":"https://openalex.org/keywords/recombinant-human-tsh","display_name":"recombinant human TSH","score":0.8679999709129333},{"id":"https://openalex.org/keywords/total-thyroxine","display_name":"total thyroxine","score":0.6520000100135803}],"concepts":[{"id":"https://openalex.org/C104488531","wikidata":"https://www.wikidata.org/wiki/Q864212","display_name":"Bioassay","level":2,"score":0.9621688723564148},{"id":"https://openalex.org/C4134927","wikidata":"https://www.wikidata.org/wiki/Q7671336","display_name":"TRH stimulation test","level":4,"score":0.701850950717926},{"id":"https://openalex.org/C176685330","wikidata":"https://www.wikidata.org/wiki/Q857638","display_name":"Radioimmunoassay","level":2,"score":0.690162181854248},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.6736017465591431},{"id":"https://openalex.org/C51296783","wikidata":"https://www.wikidata.org/wiki/Q1645498","display_name":"Microgram","level":3,"score":0.6696131825447083},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.6431493759155273},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.6086044907569885},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5973606109619141},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.49499839544296265},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4124455749988556},{"id":"https://openalex.org/C71315377","wikidata":"https://www.wikidata.org/wiki/Q11364","display_name":"Hormone","level":2,"score":0.4120391011238098},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.34529823064804077},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2601924538612366},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.2036433219909668},{"id":"https://openalex.org/C2776216936","wikidata":"https://www.wikidata.org/wiki/Q24726011","display_name":"Thyrotropin-releasing hormone","level":3,"score":0.16860336065292358},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12807074189186096},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001681","descriptor_name":"Biological Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008815","descriptor_name":"Mice, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010902","descriptor_name":"Pituitary Gland","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011863","descriptor_name":"Radioimmunoassay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013972","descriptor_name":"Thyrotropin","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D013974","descriptor_name":"Thyroxine","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D014284","descriptor_name":"Triiodothyronine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1089/thy.1995.5.55","doi":"https://doi.org/10.1089/thy.1995.5.55","is_oa":false,"landing_page_url":"https://doi.org/10.1089/thy.1995.5.55","pdf_url":null,"source":{"id":"https://openalex.org/S27569427","display_name":"Thyroid","issn_l":"1050-7256","issn":["1050-7256","1557-9077"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thyroid","raw_type":"journal-article"},{"id":"pmid:7787435","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7787435","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thyroid : official journal of the American Thyroid Association","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6351000070571899,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W1963598103","https://openalex.org/W1965798702","https://openalex.org/W1986185641","https://openalex.org/W2002975921","https://openalex.org/W2019472675","https://openalex.org/W2026779934","https://openalex.org/W2034774830","https://openalex.org/W2054462813","https://openalex.org/W2054611941","https://openalex.org/W2090950011","https://openalex.org/W2090983251","https://openalex.org/W2092640053","https://openalex.org/W2105328860","https://openalex.org/W2113537773","https://openalex.org/W2116161383","https://openalex.org/W2129232972","https://openalex.org/W2135586484","https://openalex.org/W2159791077","https://openalex.org/W2275396877"],"related_works":["https://openalex.org/W2133350242","https://openalex.org/W2103994273","https://openalex.org/W2164788717","https://openalex.org/W2069381254","https://openalex.org/W2328170023","https://openalex.org/W6991049330","https://openalex.org/W2400297094","https://openalex.org/W1996866919","https://openalex.org/W2055521733","https://openalex.org/W2001206239"],"abstract_inverted_index":{"A":[0],"new":[1],"and":[2,13,67,74,121,174],"simple":[3],"in":[4,27,144,176],"vivo":[5],"bioassay":[6,170],"suitable":[7],"for":[8,41],"routine":[9],"testing":[10],"of":[11,44,57,64,101,105,134,167,179],"pituitary":[12,66,106,180],"recombinant":[14,68,112],"TSH":[15,69,108,114,119,125,137,160,181],"preparations":[16,70],"was":[17,139],"developed.":[18],"Male":[19],"Albino":[20],"Swiss":[21],"CF-1":[22],"mice":[23,40],"were":[24,71,77],"given":[25,38],"T3":[26],"their":[28],"drinking":[29],"water":[30],"to":[31,39,53,127,141,154],"suppress":[32],"endogenous":[33],"TSH.":[34],"T3,":[35],"3.0":[36,103],"micrograms/mL,":[37],"a":[42],"period":[43],"4":[45],"days":[46],"decreased":[47],"plasma":[48],"total":[49],"T4":[50],"(TT4)":[51],"level":[52,133],"less":[54],"than":[55],"10%":[56],"the":[58,80,94,165],"nonsuppressed":[59,146],"(control)":[60],"level.":[61],"Various":[62],"doses":[63],"exogenous":[65],"injected":[72],"intraperitoneally":[73],"blood":[75],"samples":[76],"obtained":[78],"from":[79,182],"orbital":[81],"sinus":[82],"6":[83],"h":[84],"later.":[85],"The":[86,97,131,148],"TT4":[87,135],"level,":[88],"measured":[89],"by":[90],"radioimmunoassay,":[91],"served":[92],"as":[93,185,187],"assay":[95,98],"end-point.":[96],"required":[99],"injection":[100],"approximately":[102],"micrograms":[104],"human":[107,113],"(phTSH),":[109],"1.0":[110],"microgram":[111,117,123],"(rhTSH),":[115],"0.2":[116],"bovine":[118],"(bTSH),":[120],"0.1":[122],"rat":[124],"(rTSH)":[126],"attain":[128],"half-maximal":[129],"response.":[130],"maximal":[132],"after":[136],"stimulation":[138],"similar":[140],"that":[142],"observed":[143],"normal,":[145],"mice.":[147],"procedure":[149],"developed":[150],"is":[151],"relatively":[152],"easy":[153],"perform,":[155],"economical,":[156],"and,":[157],"unlike":[158],"earlier":[159],"bioassays,":[161],"does":[162],"not":[163],"require":[164],"use":[166],"radionuclides.":[168],"This":[169],"showed":[171],"acceptable":[172],"sensitivity":[173],"reliability":[175],"structure-function":[177],"studies":[178],"different":[183],"species":[184],"well":[186],"rhTSH.":[188]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}