{"id":"https://openalex.org/W1996196679","doi":"https://doi.org/10.1021/bi9826052","title":"Characterization of the Heme and Pyridoxal Phosphate Cofactors of Human Cystathionine \u03b2-Synthase Reveals Nonequivalent Active Sites","display_name":"Characterization of the Heme and Pyridoxal Phosphate Cofactors of Human Cystathionine \u03b2-Synthase Reveals Nonequivalent Active Sites","publication_year":1999,"publication_date":"1999-02-05","ids":{"openalex":"https://openalex.org/W1996196679","doi":"https://doi.org/10.1021/bi9826052","mag":"1996196679","pmid":"https://pubmed.ncbi.nlm.nih.gov/10052944"},"language":"en","primary_location":{"id":"doi:10.1021/bi9826052","doi":"https://doi.org/10.1021/bi9826052","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi9826052","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109844732","display_name":"Shinichi Taoka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shinichi Taoka","raw_affiliation_strings":["Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086141649","display_name":"Matthew West","orcid":"https://orcid.org/0000-0003-3247-1774","observed_orcids":["https://orcid.org/0000-0003-3247-1774"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew West","raw_affiliation_strings":["Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013907641","display_name":"Ruma V. Banerjee","orcid":"https://orcid.org/0000-0001-8332-3275","observed_orcids":["https://orcid.org/0000-0001-8332-3275","https://orcid.org/0000-0002-5921-4906"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ruma Banerjee","raw_affiliation_strings":["Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664","institution_ids":["https://openalex.org/I114395901"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I114395901"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.796,"has_fulltext":false,"cited_by_count":106,"citation_normalized_percentile":{"value":0.89742775,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":99},"biblio":{"volume":"38","issue":"9","first_page":"2738","last_page":"2744"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10499","display_name":"Folate and B Vitamins Research","score":0.32199999690055847,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10499","display_name":"Folate and B Vitamins Research","score":0.32199999690055847,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.1720999926328659,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12441","display_name":"Porphyrin Metabolism and Disorders","score":0.11580000072717667,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cystathionine-beta-synthase","display_name":"cystathionine beta-synthase","score":0.9959999918937683},{"id":"https://openalex.org/keywords/heme-cofactor","display_name":"heme cofactor","score":0.9580000042915344},{"id":"https://openalex.org/keywords/pyridoxal-5-phosphate","display_name":"pyridoxal phosphate","score":0.9449999928474426},{"id":"https://openalex.org/keywords/transsulfuration","display_name":"transsulfuration","score":0.8149999976158142},{"id":"https://openalex.org/keywords/co-binding","display_name":"carbon monoxide binding","score":0.8040000200271606},{"id":"https://openalex.org/keywords/cyanide-binding","display_name":"cyanide binding","score":0.8450000286102295}],"concepts":[{"id":"https://openalex.org/C16525657","wikidata":"https://www.wikidata.org/wiki/Q5201183","display_name":"Cystathionine beta synthase","level":4,"score":0.8998340368270874},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8367531895637512},{"id":"https://openalex.org/C2780859938","wikidata":"https://www.wikidata.org/wiki/Q3065731","display_name":"Pyridoxal","level":3,"score":0.6519727110862732},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.6452380418777466},{"id":"https://openalex.org/C2776217839","wikidata":"https://www.wikidata.org/wiki/Q189621","display_name":"Heme","level":3,"score":0.5886059999465942},{"id":"https://openalex.org/C2776834122","wikidata":"https://www.wikidata.org/wiki/Q418957","display_name":"Pyridoxal phosphate","level":4,"score":0.5685545206069946},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4847102165222168},{"id":"https://openalex.org/C2777090595","wikidata":"https://www.wikidata.org/wiki/Q192466","display_name":"Homocysteine","level":2,"score":0.46873411536216736},{"id":"https://openalex.org/C187566844","wikidata":"https://www.wikidata.org/wiki/Q5201182","display_name":"Cystathionine gamma-lyase","level":5,"score":0.46707016229629517},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.41947031021118164},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.31957870721817017},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.306390643119812},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.1981944739818573}],"mesh":[{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002248","descriptor_name":"Carbon Monoxide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003486","descriptor_name":"Cyanides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D003541","descriptor_name":"Cystathionine beta-Synthase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D003541","descriptor_name":"Cystathionine beta-Synthase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003541","descriptor_name":"Cystathionine beta-Synthase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006418","descriptor_name":"Heme","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011732","descriptor_name":"Pyridoxal Phosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012694","descriptor_name":"Serine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1021/bi9826052","doi":"https://doi.org/10.1021/bi9826052","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi9826052","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:10052944","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10052944","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmid:10353853","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10353853","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.4043000042438507}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2820432670","display_name":null,"funder_award_id":"HL58984","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G5534758678","display_name":"Regulation of H2S Biogenesis","funder_award_id":"R01HL058984","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1549590649","https://openalex.org/W1563387341","https://openalex.org/W1785592597","https://openalex.org/W1982341626","https://openalex.org/W1998130783","https://openalex.org/W2036965742","https://openalex.org/W2070085194","https://openalex.org/W2077993780","https://openalex.org/W2090291630","https://openalex.org/W2108484297"],"related_works":["https://openalex.org/W2139680667","https://openalex.org/W2019834611","https://openalex.org/W1999026988","https://openalex.org/W7052863860","https://openalex.org/W1989649013","https://openalex.org/W2047756670","https://openalex.org/W1982341626","https://openalex.org/W1563387341","https://openalex.org/W1974102591","https://openalex.org/W2060483499"],"abstract_inverted_index":{"Cystathionine":[0],"beta-synthase":[1,116,152,184,269,324],"is":[2,41,67,109,122,137,185,214],"an":[3,287],"unusual":[4],"enzyme":[5,54],"that":[6,132,160,241,316],"requires":[7],"the":[8,17,48,59,62,65,75,81,84,87,90,98,105,129,133,139,161,172,194,203,208,220,224,230,246,256,260,293,305,314,317],"cofactors":[9],"heme":[10,66,85,318],"and":[11,21,108,147,158,175,188,196,205,278,312,319],"pyridoxal":[12,60],"phosphate":[13],"(PLP)":[14],"to":[15,23,83,104,126,149,170,182,219,245,249,263,303,308,313],"catalyze":[16],"condensation":[18],"of":[19,31,38,64,77,80,89,93,100,114,141,144,193,212,223,232,259,267,283,295],"homocysteine":[20,134],"serine":[22,213],"generate":[24],"cystathionine.":[25],"This":[26],"transsulfuration":[27],"reaction":[28,50],"represents":[29],"one":[30,79],"two":[32,155,273,296],"major":[33],"cellular":[34],"routes":[35],"for":[36,45,58,286],"detoxification":[37],"homocysteine,":[39,127],"which":[40],"a":[42,56,101,191,217,233,250],"risk":[43],"factor":[44],"atherosclerosis.":[46],"While":[47],"beta-replacement":[49],"catalyzed":[51],"by":[52,111,120,216],"this":[53,70],"suggests":[55],"role":[57,63],"phosphate,":[61],"uncertain.":[68],"In":[69],"study":[71],"we":[72],"have":[73,166],"examined":[74],"effect":[76],"changing":[78],"ligands":[82],"on":[86],"activity":[88],"enzyme.":[91],"Binding":[92,143,211],"carbon":[94],"monooxide":[95],"results":[96,279,291],"in":[97,138,154,280,292,322],"displacement":[99],"thiolate":[102],"ligand":[103],"ferrous":[106,150],"heme,":[107],"accompanied":[110,215],"complete":[112,281],"loss":[113,282],"cystathionine":[115,151,183,264,268,323],"activity.":[117,284],"Furthermore,":[118],"inhibition":[119],"CO":[121,146],"competitive":[123],"with":[124,178,200,270],"respect":[125],"providing":[128],"first":[130,257],"indication":[131],"binding":[135],"site":[136],"proximity":[140],"heme.":[142],"both":[145],"cyanide":[148],"occurs":[153],"distinct":[156],"isotherms":[157],"indicates":[159],"hemes":[162],"are":[163,325],"nonequivalent.":[164,326],"We":[165],"employed":[167],"fluorescence":[168],"spectroscopy":[169],"characterize":[171],"bound":[173,181,262],"PLP":[174,180,261,306,320],"its":[176],"interaction":[177],"serine.":[179],"weakly":[186],"fluorescent":[187,235],"exists":[189],"as":[190,227,229],"mixture":[192],"protonated":[195],"unprotonated":[197,221],"tautomers.":[198],"Reaction":[199],"hydroxylamine":[201,271],"releases":[202,272],"oxime":[204],"greatly":[206],"enhances":[207],"associated":[209],"fluorescence.":[210],"shift":[218],"tautomer":[222],"external":[225],"aldimine":[226],"well":[228],"appearance":[231],"new":[234],"species":[236],"at":[237],"approximately":[238],"400":[239],"nm":[240],"could":[242],"be":[243],"due":[244],"aminoacrylate":[247],"or":[248],"gemdiamine":[251],"intermediate.":[252],"These":[253,299],"data":[254,300],"provide":[255],"characterization":[258],"beta-synthase.":[265],"Treatment":[266],"PLPs":[274],"after":[275],"1":[276],"day":[277],"Incubation":[285],"additional":[288],"3-4":[289],"days":[290],"release":[294],"more":[297],"PLPs.":[298],"lead":[301],"us":[302],"revise":[304],"stoichiometry":[307],"4":[309],"per":[310],"tetramer,":[311],"conclusion":[315],"sites":[321]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":9}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}