{"id":"https://openalex.org/W1995823402","doi":"https://doi.org/10.1046/j.1365-2370.2003.00376.x","title":"Relative quantification of HLA\u2010DRA1 and \u2010DQA1 expression by real\u2010time reverse transcriptase\u2013polymerase chain reaction (RT\u2013PCR)","display_name":"Relative quantification of HLA\u2010DRA1 and \u2010DQA1 expression by real\u2010time reverse transcriptase\u2013polymerase chain reaction (RT\u2013PCR)","publication_year":2003,"publication_date":"2003-03-19","ids":{"openalex":"https://openalex.org/W1995823402","doi":"https://doi.org/10.1046/j.1365-2370.2003.00376.x","mag":"1995823402","pmid":"https://pubmed.ncbi.nlm.nih.gov/12648283"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-2370.2003.00376.x","doi":"https://doi.org/10.1046/j.1365-2370.2003.00376.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2370.2003.00376.x","pdf_url":null,"source":{"id":"https://openalex.org/S116380431","display_name":"European Journal of Immunogenetics","issn_l":"0960-7420","issn":["0960-7420","1365-2370"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Immunogenetics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"S. Fernandez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I181369854","display_name":"Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg","ror":"https://ror.org/00f7hpc57","country_code":"DE","type":"education","lineage":["https://openalex.org/I181369854"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"S. Fernandez","raw_affiliation_strings":["Institute for Clinical Immunology, Department of Medicine III, Friedrich Alexander University, Erlangen-Nuremberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Clinical Immunology, Department of Medicine III, Friedrich Alexander University, Erlangen-Nuremberg, Germany","institution_ids":["https://openalex.org/I181369854"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087250571","display_name":"Ralf Wa\u00dfmuth","orcid":"https://orcid.org/0000-0002-9481-3533","observed_orcids":["https://orcid.org/0000-0002-9481-3533"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R. Wassmuth","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006966089","display_name":"Ina Knerr","orcid":"https://orcid.org/0000-0002-4015-0658","observed_orcids":["https://orcid.org/0000-0002-4015-0658"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"I. Knerr","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113607986","display_name":"Camille H. Nicolas Frank","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C. Frank","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5037543707","display_name":"Johannes\u2010Peter Haas","orcid":"https://orcid.org/0000-0001-8117-1298","observed_orcids":["https://orcid.org/0000-0001-8117-1298"]},"institutions":[{"id":"https://openalex.org/I36522303","display_name":"Universit\u00e4t Greifswald","ror":"https://ror.org/00r1edq15","country_code":"DE","type":"education","lineage":["https://openalex.org/I36522303"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"J. P. Haas","raw_affiliation_strings":["Johannes-Peter Haas, Department of Paediatrics, Division of Neonatology and Critical Care Medicine, University of Greifswald, Soldtmannstr. 15, D17487 Greifswald, Germany. Tel: 49 3834 866409; Fax: 49 3834 867377; E-mail:"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Johannes-Peter Haas, Department of Paediatrics, Division of Neonatology and Critical Care Medicine, University of Greifswald, Soldtmannstr. 15, D17487 Greifswald, Germany. Tel: 49 3834 866409; Fax: 49 3834 867377; E-mail:","institution_ids":["https://openalex.org/I36522303"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5037543707"],"corresponding_institution_ids":["https://openalex.org/I36522303"],"apc_list":null,"apc_paid":null,"fwci":1.7615,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.83160124,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"30","issue":"2","first_page":"141","last_page":"148"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10261","display_name":"Genetic Associations and Epidemiology","score":0.5746999979019165,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10261","display_name":"Genetic Associations and Epidemiology","score":0.5746999979019165,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.3237000107765198,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10137","display_name":"Multiple Sclerosis Research Studies","score":0.012600000016391277,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hla-dqa1","display_name":"HLA-DQA1","score":0.9810000061988831},{"id":"https://openalex.org/keywords/mhc-class-ii","display_name":"MHC class II","score":0.7760000228881836},{"id":"https://openalex.org/keywords/promoter-polymorphism","display_name":"promoter polymorphism","score":0.7689999938011169},{"id":"https://openalex.org/keywords/allele-specific-expression","display_name":"allele-specific expression","score":0.8059999942779541},{"id":"https://openalex.org/keywords/mrna-quantification","display_name":"mRNA quantification","score":0.7609999775886536},{"id":"https://openalex.org/keywords/taqman-real-time-rt-pcr","display_name":"TaqMan real-time RT-PCR","score":0.8529999852180481},{"id":"https://openalex.org/keywords/peripheral-blood-mononuclear-cells","display_name":"peripheral blood mononuclear cells","score":0.8460000157356262}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8022786974906921},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7408613562583923},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.6770787835121155},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.6382733583450317},{"id":"https://openalex.org/C143660080","wikidata":"https://www.wikidata.org/wiki/Q7684883","display_name":"TaqMan","level":4,"score":0.6304316520690918},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.5967868566513062},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.520717442035675},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.506609320640564},{"id":"https://openalex.org/C4692481","wikidata":"https://www.wikidata.org/wiki/Q169526","display_name":"Reverse transcription polymerase chain reaction","level":4,"score":0.5002861022949219},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.49514880776405334},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.49015745520591736},{"id":"https://openalex.org/C188280979","wikidata":"https://www.wikidata.org/wiki/Q911125","display_name":"Human leukocyte antigen","level":3,"score":0.4614374339580536},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.28923147916793823},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.22462618350982666},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.10179111361503601},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D006683","descriptor_name":"HLA-DQ Antigens","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006683","descriptor_name":"HLA-DQ Antigens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006684","descriptor_name":"HLA-DR Antigens","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006684","descriptor_name":"HLA-DR Antigens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D059805","descriptor_name":"HLA-DR alpha-Chains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059848","descriptor_name":"HLA-DQ alpha-Chains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-2370.2003.00376.x","doi":"https://doi.org/10.1046/j.1365-2370.2003.00376.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2370.2003.00376.x","pdf_url":null,"source":{"id":"https://openalex.org/S116380431","display_name":"European Journal of Immunogenetics","issn_l":"0960-7420","issn":["0960-7420","1365-2370"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Immunogenetics","raw_type":"journal-article"},{"id":"pmid:12648283","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12648283","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.428600013256073,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W173128576","https://openalex.org/W229119023","https://openalex.org/W1590407189","https://openalex.org/W1973086287","https://openalex.org/W1989877989","https://openalex.org/W1990535894","https://openalex.org/W2006124126","https://openalex.org/W2014108306","https://openalex.org/W2032915456","https://openalex.org/W2041089253","https://openalex.org/W2050150716","https://openalex.org/W2051005480","https://openalex.org/W2053983761","https://openalex.org/W2063476877","https://openalex.org/W2082191940","https://openalex.org/W2094200627","https://openalex.org/W2103081500","https://openalex.org/W2114173932","https://openalex.org/W2133874072","https://openalex.org/W2145028661","https://openalex.org/W2145684092","https://openalex.org/W2160788240","https://openalex.org/W2164578725","https://openalex.org/W2425462129","https://openalex.org/W2917553822"],"related_works":["https://openalex.org/W2017396422","https://openalex.org/W2053983761","https://openalex.org/W2360852084","https://openalex.org/W1833798192","https://openalex.org/W1972112995","https://openalex.org/W2037525473","https://openalex.org/W2770570123","https://openalex.org/W2752507294","https://openalex.org/W6885038505","https://openalex.org/W4234086293"],"abstract_inverted_index":{"Polymorphism":[0],"in":[1,30,37,39,58,263],"the":[2,8,31,44,63,101,136,158,210,215,231,237,255],"upstream":[3],"regulatory":[4],"region":[5,34],"(URR)":[6],"of":[7,65,88,95,103,135,146,161,191,209,218,233,236,243,257,260],"MHC":[9],"class":[10],"II":[11],"DQA1":[12,89,212,219,238,244,261],"gene":[13,41,60,245],"defines":[14],"10":[15],"different":[16],"alleles":[17,235],"named":[18],"QAP":[19],"(DQA1":[20],"promoter).":[21],"In":[22,43,156],"vitro":[23],"studies":[24],"have":[25],"suggested":[26],"that":[27,224],"allelic":[28],"polymorphism":[29],"HLA-DQA":[32],"promoter":[33],"may":[35],"result":[36],"differences":[38,57],"HLA-DQA1":[40,59,165],"expression.":[42,61],"present":[45],"study,":[46],"we":[47],"used":[48],"real-time":[49,129,251],"reverse":[50,68,85],"transcriptase-polymerase":[51],"chain":[52],"reaction":[53],"(RT-PCR)":[54],"to":[55],"quantify":[56],"After":[62],"isolation":[64],"total":[66,159,216],"mRNA,":[67],"transcription":[69],"into":[70],"cDNA":[71,147],"was":[72,111,149,169,176,200,227],"carried":[73,112],"out":[74,113],"using":[75,92,178],"random":[76],"hexamer":[77],"priming":[78],"and":[79,109,131,166,180,184,220,265],"moloney":[80],"murine":[81],"leukaemia":[82],"virus":[83],"(MMLV)":[84],"transcriptase.":[86],"Quantification":[87],"mRNA":[90,162,222],"species":[91],"a":[93],"set":[94],"six":[96],"group-specific":[97],"primer":[98],"pairs":[99],"for":[100,171,254],"detection":[102,130],"HLA-DQA1*01,":[104],"*02,":[105],"*03,":[106],"*04,":[107],"*05":[108],"*06":[110],"on":[114],"an":[115],"ABI":[116],"PRISM":[117],"GeneAmp":[118],"7700":[119],"Sequence":[120],"Detection":[121],"System":[122],"(Perkin":[123,140],"Elmer,":[124,141],"Foster":[125,142],"City,":[126,143],"CA)":[127],"with":[128,186,202,214,230],"quantification":[132,242],"taking":[133],"advantage":[134],"fluorescence":[137],"TaqMan":[138],"technology":[139],"CA).":[144],"Normalization":[145],"templates":[148],"achieved":[150,249],"by":[151,164,250],"glyceraldehyde-3-phosphate":[152],"dehydrogenase":[153],"(GAPDH)":[154],"quantification.":[155],"addition,":[157],"amount":[160],"produced":[163],"HLA-DRA1":[167],"expression":[168,213,217,226,232,259],"quantified":[170],"comparison.":[172],"Subsequently,":[173],"this":[174],"approach":[175],"validated":[177],"Raji":[179],"HUT-78":[181],"cell":[182],"lines":[183],"tested":[185],"peripheral":[187],"mononuclear":[188],"cells":[189],"(PBMC)":[190],"45":[192],"samples":[193],"taken":[194],"from":[195],"healthy":[196],"volunteers.":[197],"The":[198],"sensitivity":[199],"determined":[201],">":[203],"or":[204],"=":[205],"10(2)":[206],"copies.":[207],"Comparison":[208],"allele-specific":[211,241],"DRA1":[221],"indicated":[223],"DQA1*04":[225],"increased":[228],"compared":[229],"other":[234],"gene.":[239],"Thus,":[240],"products":[246],"could":[247],"be":[248],"RT-PCR":[252],"suitable":[253],"analysis":[256],"differential":[258],"mRNAs":[262],"homozygote":[264],"heterozygote":[266],"combinations.":[267]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
