{"id":"https://openalex.org/W1995800866","doi":"https://doi.org/10.3791/50750","title":"Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.","display_name":"Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.","publication_year":2013,"publication_date":"2013-11-01","ids":{"openalex":"https://openalex.org/W1995800866","doi":"https://doi.org/10.3791/50750","mag":"1995800866","pmid":"https://pubmed.ncbi.nlm.nih.gov/24300796","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3969900"},"language":"en","primary_location":{"id":"doi:10.3791/50750","doi":"https://doi.org/10.3791/50750","is_oa":false,"landing_page_url":"https://doi.org/10.3791/50750","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3969900","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024740911","display_name":"Michelle T. Juarez","orcid":"https://orcid.org/0000-0002-6910-0881","observed_orcids":["https://orcid.org/0000-0002-6910-0881"]},"institutions":[{"id":"https://openalex.org/I125687163","display_name":"City College of New York","ror":"https://ror.org/00wmhkr98","country_code":"US","type":"education","lineage":["https://openalex.org/I125687163"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michelle T. Juarez","raw_affiliation_strings":["Sophie Davis School of Biomedical Education, The City College of New York"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sophie Davis School of Biomedical Education, The City College of New York","institution_ids":["https://openalex.org/I125687163"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080940430","display_name":"Rachel Patterson","orcid":"https://orcid.org/0000-0003-0131-4178","observed_orcids":["https://orcid.org/0000-0003-0131-4178"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rachel A. Patterson","raw_affiliation_strings":["Cell & Developmental Biology, University of California, San Diego"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell & Developmental Biology, University of California, San Diego","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101687896","display_name":"Wilson Li","orcid":"https://orcid.org/0000-0003-3954-1990","observed_orcids":["https://orcid.org/0000-0003-3954-1990"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wilson Li","raw_affiliation_strings":["Cell & Developmental Biology, University of California, San Diego"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell & Developmental Biology, University of California, San Diego","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5032347452","display_name":"William J. McGinnis","orcid":"https://orcid.org/0000-0001-8474-0388","observed_orcids":["https://orcid.org/0000-0001-8474-0388"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William McGinnis","raw_affiliation_strings":["Cell & Developmental Biology, University of California, San Diego"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell & Developmental Biology, University of California, San Diego","institution_ids":["https://openalex.org/I36258959"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.7049,"has_fulltext":true,"cited_by_count":11,"citation_normalized_percentile":{"value":0.73122375,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":null,"issue":"81","first_page":"e50750","last_page":"e50750"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10771","display_name":"Wound Healing and Treatments","score":0.8115000128746033,"subfield":{"id":"https://openalex.org/subfields/2742","display_name":"Rehabilitation"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10771","display_name":"Wound Healing and Treatments","score":0.8115000128746033,"subfield":{"id":"https://openalex.org/subfields/2742","display_name":"Rehabilitation"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10423","display_name":"Neurobiology and Insect Physiology Research","score":0.07859999686479568,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11403","display_name":"Invertebrate Immune Response Mechanisms","score":0.03319999948143959,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/drosophila-embryo","display_name":"Drosophila embryo","score":0.9039999842643738},{"id":"https://openalex.org/keywords/wound-healing","display_name":"wound healing","score":0.7200000286102295},{"id":"https://openalex.org/keywords/puncture-injury","display_name":"puncture injury","score":0.8410000205039978},{"id":"https://openalex.org/keywords/microinjection","display_name":"microinjection","score":0.6029999852180481},{"id":"https://openalex.org/keywords/embryonic-development","display_name":"embryonic development","score":0.5600000023841858},{"id":"https://openalex.org/keywords/innate-immunity","display_name":"innate immunity","score":0.8109999895095825},{"id":"https://openalex.org/keywords/epidermal-regeneration","display_name":"epidermal regeneration","score":0.8009999990463257}],"concepts":[{"id":"https://openalex.org/C2780269544","wikidata":"https://www.wikidata.org/wiki/Q1509074","display_name":"Wound healing","level":2,"score":0.7345137596130371},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6992028951644897},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6585671901702881},{"id":"https://openalex.org/C167290458","wikidata":"https://www.wikidata.org/wiki/Q2035755","display_name":"Drosophila embryogenesis","level":4,"score":0.5601158738136292},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.5540117025375366},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.45260658860206604},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3094961643218994},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.2624374032020569},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1971701979637146},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16375887393951416}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004330","descriptor_name":"Drosophila","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":true},{"descriptor_ui":"D004330","descriptor_name":"Drosophila","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004817","descriptor_name":"Epidermis","qualifier_ui":"Q000293","qualifier_name":"injuries","is_major_topic":true},{"descriptor_ui":"D004817","descriptor_name":"Epidermis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D012038","descriptor_name":"Regeneration","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D014945","descriptor_name":"Wound Healing","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014945","descriptor_name":"Wound Healing","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D023421","descriptor_name":"Models, Animal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.3791/50750","doi":"https://doi.org/10.3791/50750","is_oa":false,"landing_page_url":"https://doi.org/10.3791/50750","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2969388","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3969900","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3969900","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3969900","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Vis Exp","raw_type":"Text"},{"id":"pmid:24300796","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24300796","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"},{"id":"pmh:oai:academicworks.cuny.edu:cc_pubs-1107","doi":null,"is_oa":false,"landing_page_url":"https://academicworks.cuny.edu/cc_pubs/108","pdf_url":null,"source":{"id":"https://openalex.org/S4377196304","display_name":"CUNY Academic Works (City University of New York)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I174216632","host_organization_name":"City University of New York","host_organization_lineage":["https://openalex.org/I174216632"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Publications and Research","raw_type":"article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:2969388","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3969900","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1105823858","display_name":"Regulation of epidermal barrier wound repair","funder_award_id":"R01GM077197","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G1746537960","display_name":"Cellular/Molecular Basis of Development: Research Center","funder_award_id":"G12MD007603","funder_id":"https://openalex.org/F4320337534","funder_display_name":"National Institute on Minority Health and Health Disparities"},{"id":"https://openalex.org/G3099117808","display_name":null,"funder_award_id":"8G12MD7603-27","funder_id":"https://openalex.org/F4320337534","funder_display_name":"National Institute on Minority Health and Health Disparities"},{"id":"https://openalex.org/G7304647480","display_name":"Cellular/Molecular Basis of Development: Research Center","funder_award_id":"G12RR003060","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G8801402897","display_name":"Specificity in Homeotic Response Elements","funder_award_id":"R37HD028315","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G9000881089","display_name":"San Diego IRACDA Scholars Program","funder_award_id":"K12GM068524","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"},{"id":"https://openalex.org/F4320337534","display_name":"National Institute on Minority Health and Health Disparities","ror":"https://ror.org/0493hgw16"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1486725077","https://openalex.org/W1968050693","https://openalex.org/W1972504543","https://openalex.org/W1985207173","https://openalex.org/W1992995602","https://openalex.org/W2013727495","https://openalex.org/W2014378121","https://openalex.org/W2024816720","https://openalex.org/W2056240884","https://openalex.org/W2059511014","https://openalex.org/W2060756220","https://openalex.org/W2062269693","https://openalex.org/W2065546150","https://openalex.org/W2076301035","https://openalex.org/W2085293124","https://openalex.org/W2085399135","https://openalex.org/W2094760633","https://openalex.org/W2096060868","https://openalex.org/W2107638340","https://openalex.org/W2110643873","https://openalex.org/W2122277738","https://openalex.org/W2142227317","https://openalex.org/W4240786157","https://openalex.org/W4407723420"],"related_works":["https://openalex.org/W12591472","https://openalex.org/W2811079910","https://openalex.org/W3175868593","https://openalex.org/W2314452054","https://openalex.org/W2059511014","https://openalex.org/W1431155663","https://openalex.org/W1547271135","https://openalex.org/W2076301035","https://openalex.org/W1246174232","https://openalex.org/W2997207877"],"abstract_inverted_index":{"The":[0,125,153,193],"Drosophila":[1,133,154,202,207,242],"embryo":[2],"develops":[3],"a":[4,17,34,39,54,160,169,181,203],"robust":[5],"epidermal":[6,41,155,215,245],"layer":[7],"that":[8,44,70,116,174,189,225],"serves":[9],"both":[10],"to":[11,24,37,76,106,150,163,183,212,235,241],"protect":[12],"the":[13,72,77,132,139,147,165,214,219],"internal":[14],"cells":[15],"from":[16,122],"harsh":[18],"external":[19],"environment":[20],"as":[21,23,97,178,180],"well":[22,179],"maintain":[25],"cellular":[26],"homeostasis.":[27],"Puncture":[28,63],"injury":[29,66,86],"with":[30,218],"glass":[31],"needles":[32],"provides":[33,68,159,231],"direct":[35],"method":[36],"trigger":[38],"rapid":[40],"wound":[42,46,78,126,156,176,191,209],"response":[43,157],"activates":[45],"transcriptional":[47],"reporters,":[48],"which":[49,230],"can":[50],"be":[51],"visualized":[52],"by":[53],"localized":[55],"reporter":[56],"signal":[57],"in":[58,87,112,223],"living":[59],"embryos":[60,90,104,109,140,149],"or":[61,64],"larvae.":[62],"laser":[65],"also":[67],"signals":[69],"promote":[71,175,190],"recruitment":[73],"of":[74,101,120,131,138,146,168,171],"hemocytes":[75],"site.":[79],"Surprisingly,":[80],"severe":[81],"(through":[82],"and":[83,144,197,243],"through)":[84],"puncture":[85,123],"late":[88],"stage":[89],"only":[91],"rarely":[92],"disrupts":[93],"normal":[94],"embryonic":[95],"development,":[96],"greater":[98],"than":[99],"90%":[100],"such":[102],"wounded":[103],"survive":[105],"adulthood":[107],"when":[108],"are":[110,226],"injected":[111],"an":[113,232],"oil":[114],"medium":[115],"minimizes":[117],"immediate":[118],"leakage":[119],"hemolymph":[121],"sites.":[124],"procedure":[127],"does":[128],"require":[129],"micromanipulation":[130],"embryos,":[134],"including":[135],"manual":[136],"alignment":[137],"on":[141],"agar":[142],"plates":[143],"transfer":[145],"aligned":[148],"microscope":[151],"slides.":[152],"assay":[158],"quick":[161],"system":[162,234],"test":[164],"genetic":[166],"requirements":[167],"variety":[170],"biological":[172],"functions":[173],"healing,":[177],"way":[182],"screen":[184],"for":[185],"potential":[186],"chemical":[187],"compounds":[188],"healing.":[192],"short":[194],"life":[195],"cycle":[196],"easy":[198],"culturing":[199],"routine":[200],"make":[201],"powerful":[204],"model":[205],"organism.":[206],"clean":[208],"healing":[210],"appears":[211],"coordinate":[213],"regenerative":[216],"response,":[217,222],"innate":[220],"immune":[221],"ways":[224],"still":[227],"under":[228],"investigation,":[229],"excellent":[233],"find":[236],"conserved":[237],"regulatory":[238],"mechanisms":[239],"common":[240],"mammalian":[244],"wounding.":[246]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}