{"id":"https://openalex.org/W1995671776","doi":"https://doi.org/10.1117/12.424546","title":"Chronic imaging of amyloid plaques in the live mouse brain using multiphoton microscopy","display_name":"Chronic imaging of amyloid plaques in the live mouse brain using multiphoton microscopy","publication_year":2001,"publication_date":"2001-04-24","ids":{"openalex":"https://openalex.org/W1995671776","doi":"https://doi.org/10.1117/12.424546","mag":"1995671776"},"language":"en","primary_location":{"id":"doi:10.1117/12.424546","doi":"https://doi.org/10.1117/12.424546","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.424546","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059117241","display_name":"Brian J. Bacskai","orcid":"https://orcid.org/0000-0002-2584-6702","observed_orcids":["https://orcid.org/0000-0002-2584-6702"]},"institutions":[{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian J. Bacskai","raw_affiliation_strings":["Massachusetts General Hospital (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Massachusetts General Hospital (United States)","institution_ids":["https://openalex.org/I4210087915"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053048569","display_name":"Stephen T. Kajdasz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen T. Kajdasz","raw_affiliation_strings":["Massachusetts General Hospital (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Massachusetts General Hospital (United States)","institution_ids":["https://openalex.org/I4210087915"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058434888","display_name":"R. H. Christie","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. H. Christie","raw_affiliation_strings":["Massachusetts General Hospital (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Massachusetts General Hospital (United States)","institution_ids":["https://openalex.org/I4210087915"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066282020","display_name":"Warren R. Zipfel","orcid":"https://orcid.org/0000-0003-2640-329X","observed_orcids":["https://orcid.org/0000-0003-2640-329X"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Warren R. Zipfel","raw_affiliation_strings":["Cornell Univ. United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cornell Univ. United States","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008812067","display_name":"Rebecca M. Williams","orcid":"https://orcid.org/0000-0002-1391-5846","observed_orcids":["https://orcid.org/0000-0002-1391-5846"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rebecca M. Williams","raw_affiliation_strings":["Cornell Univ. United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cornell Univ. United States","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034498728","display_name":"Karl A. Kasischke","orcid":"https://orcid.org/0000-0003-0582-4319","observed_orcids":["https://orcid.org/0000-0003-0582-4319"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karl A. Kasischke","raw_affiliation_strings":["Cornell Univ. United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cornell Univ. United States","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111539887","display_name":"Watt W. Webb","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Watt W. Webb","raw_affiliation_strings":["Cornell Univ. United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cornell Univ. United States","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5068688487","display_name":"Bradley T. Hyman","orcid":"https://orcid.org/0000-0002-7959-9401","observed_orcids":["https://orcid.org/0000-0002-7959-9401"]},"institutions":[{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"B. T. Hyman","raw_affiliation_strings":["Massachusetts General Hospital (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Massachusetts General Hospital (United States)","institution_ids":["https://openalex.org/I4210087915"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":6.7045,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.93887946,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"4262","issue":null,"first_page":"125","last_page":"125"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.6606000065803528,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.6606000065803528,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.3253999948501587,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/amyloid-plaques","display_name":"amyloid plaques","score":0.9950000047683716},{"id":"https://openalex.org/keywords/amyloid-beta","display_name":"amyloid-beta","score":0.8140000104904175},{"id":"https://openalex.org/keywords/multiphoton-microscopy","display_name":"multiphoton microscopy","score":0.7979999780654907},{"id":"https://openalex.org/keywords/thioflavine-s","display_name":"thioflavine S","score":0.8460000157356262},{"id":"https://openalex.org/keywords/cortical-amyloid-deposition","display_name":"cortical amyloid deposition","score":0.6520000100135803},{"id":"https://openalex.org/keywords/multicolor-imaging","display_name":"multicolor imaging","score":0.7639999985694885},{"id":"https://openalex.org/keywords/microglia","display_name":"microglia","score":0.8410000205039978},{"id":"https://openalex.org/keywords/hoechst-dye","display_name":"Hoechst dye","score":0.7929999828338623},{"id":"https://openalex.org/keywords/tomato-lectin","display_name":"tomato lectin","score":0.8669999837875366}],"concepts":[{"id":"https://openalex.org/C2777633098","wikidata":"https://www.wikidata.org/wiki/Q2736051","display_name":"Amyloid (mycology)","level":2,"score":0.6290425062179565},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.6204201579093933},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.6047627925872803},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.6025444865226746},{"id":"https://openalex.org/C2779830541","wikidata":"https://www.wikidata.org/wiki/Q1622829","display_name":"Microglia","level":3,"score":0.574516236782074},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.4978492259979248},{"id":"https://openalex.org/C29331040","wikidata":"https://www.wikidata.org/wiki/Q7239554","display_name":"Preclinical imaging","level":3,"score":0.44480520486831665},{"id":"https://openalex.org/C47785574","wikidata":"https://www.wikidata.org/wiki/Q373859","display_name":"Two-photon excitation microscopy","level":3,"score":0.4433954060077667},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44117119908332825},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.42599067091941833},{"id":"https://openalex.org/C2777348757","wikidata":"https://www.wikidata.org/wiki/Q2346577","display_name":"Cortex (anatomy)","level":2,"score":0.4238031208515167},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4144085943698883},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.41251468658447266},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3246542811393738},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.3180822730064392},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.22815263271331787},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.22091412544250488},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14108943939208984},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.13772261142730713},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.13300856947898865},{"id":"https://openalex.org/C2776914184","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammation","level":2,"score":0.10403555631637573},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1117/12.424546","doi":"https://doi.org/10.1117/12.424546","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.424546","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8560000061988831,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320337337","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1974889710","https://openalex.org/W1252751620","https://openalex.org/W76631440","https://openalex.org/W2188718361","https://openalex.org/W2103558105","https://openalex.org/W2189276344","https://openalex.org/W25859199","https://openalex.org/W2072846848","https://openalex.org/W3003588588","https://openalex.org/W1969490969"],"abstract_inverted_index":{"Transgenic":[0],"mice":[1,178],"expressing":[2],"the":[3,21,43,74,87,183,189],"human":[4,22],"Amyloid":[5],"Precursor":[6],"Protein":[7],"(APP)":[8],"develop":[9],"amyloid":[10,38,59,66],"plaques":[11,16,67,91],"as":[12,54],"they":[13],"age.":[14],"These":[15,152],"resemble":[17],"those":[18],"found":[19],"in":[20,42,128,166,175,188],"disease.":[23],"Multiphoton":[24,61],"laser":[25],"scanning":[26],"microscopy":[27],"combined":[28],"with":[29,163],"a":[30,55,116,171],"novel":[31],"surgical":[32,77],"approach":[33],"was":[34,52],"used":[35,53],"to":[36,69,86,104,156],"measure":[37],"plaque":[39,109],"dynamics":[40,184],"chronically":[41],"cortex":[44],"of":[45,58,65,101,118,142,170,173,185],"living":[46],"transgenic":[47,177],"mice.":[48],"Thioflavine":[49],"S":[50],"(thioS)":[51],"fluorescent":[56],"marker":[57],"deposits.":[60],"excitation":[62],"allowed":[63],"visualization":[64],"up":[68],"200":[70],"micrometers":[71],"deep":[72],"into":[73,182],"brain.":[75,190],"The":[76],"site":[78],"could":[79,95],"be":[80,96],"imaged":[81],"repeatedly":[82],"without":[83],"overt":[84],"damage":[85],"tissue,":[88],"and":[89,134,148],"individual":[90],"within":[92],"this":[93],"volume":[94],"reliably":[97],"identified":[98],"over":[99,113],"periods":[100],"several":[102,105],"days":[103],"months.":[106],"On":[107],"average,":[108],"sizes":[110],"remained":[111],"constant":[112],"time,":[114],"supporting":[115],"model":[117],"rapid":[119],"deposition,":[120],"followed":[121],"by":[122],"relative":[123],"stability.":[124],"Alternative":[125],"reporters":[126,174],"for":[127],"vivo":[129],"histology":[130],"include":[131],"thiazine":[132],"red,":[133],"FITC-labeled":[135,149],"amyloid-(Beta)":[136,164,186],"peptide.":[137],"We":[138],"also":[139],"present":[140],"examples":[141],"multi-color":[143],"imaging":[144,169],"using":[145],"Hoechst":[146],"dyes":[147],"tomato":[150],"lectin.":[151],"approaches":[153],"allow":[154],"us":[155],"observe":[157],"cell":[158],"nuclei":[159],"or":[160],"microglia":[161],"simultaneously":[162],"deposits":[165],"vivo.":[167],"Chronic":[168],"variety":[172],"these":[176],"should":[179],"provide":[180],"insight":[181],"activity":[187]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}