{"id":"https://openalex.org/W1995609060","doi":"https://doi.org/10.1021/bi00431a001","title":"Human leukocyte and porcine pancreatic elastase: x-ray crystal structures, mechanism, substrate specificity, and mechanism-based inhibitors","display_name":"Human leukocyte and porcine pancreatic elastase: x-ray crystal structures, mechanism, substrate specificity, and mechanism-based inhibitors","publication_year":1989,"publication_date":"1989-03-07","ids":{"openalex":"https://openalex.org/W1995609060","doi":"https://doi.org/10.1021/bi00431a001","mag":"1995609060","pmid":"https://pubmed.ncbi.nlm.nih.gov/2655701"},"language":"en","primary_location":{"id":"doi:10.1021/bi00431a001","doi":"https://doi.org/10.1021/bi00431a001","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00431a001","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109009178","display_name":"Wolfram Bode","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210150093","display_name":"Max Planck Institute of Biochemistry","ror":"https://ror.org/04py35477","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210150093"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Wolfram Bode","raw_affiliation_strings":["Max-Planck-Institut f\u00fcr Biochemie, Martinsried, West Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max-Planck-Institut f\u00fcr Biochemie, Martinsried, West Germany","institution_ids":["https://openalex.org/I4210150093"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Edgar Meyer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Edgar Meyer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5015489808","display_name":"James C. Powers","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"James C. Powers","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":12.0884,"has_fulltext":false,"cited_by_count":400,"citation_normalized_percentile":{"value":0.9914045,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":100},"biblio":{"volume":"28","issue":"5","first_page":"1951","last_page":"1963"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.8476999998092651,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.8476999998092651,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.15049999952316284,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/elastase","display_name":"elastase","score":0.8230000138282776},{"id":"https://openalex.org/keywords/neutrophil-elastase","display_name":"neutrophil elastase","score":0.9020000100135803},{"id":"https://openalex.org/keywords/porcine-pancreatic-elastase","display_name":"porcine pancreatic elastase","score":0.9129999876022339},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.796999990940094},{"id":"https://openalex.org/keywords/enzyme-mechanism","display_name":"enzyme mechanism","score":0.7080000042915344},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.9990000128746033},{"id":"https://openalex.org/keywords/mechanism-based-inhibitors","display_name":"mechanism-based inhibitors","score":0.9700000286102295},{"id":"https://openalex.org/keywords/serine-protease","display_name":"serine protease","score":0.6740000247955322},{"id":"https://openalex.org/keywords/enzyme-catalysis","display_name":"enzyme catalysis","score":0.3330000042915344}],"concepts":[{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.7682462334632874},{"id":"https://openalex.org/C89611455","wikidata":"https://www.wikidata.org/wiki/Q6804646","display_name":"Mechanism (biology)","level":2,"score":0.5936322808265686},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5568634867668152},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.4385257065296173},{"id":"https://openalex.org/C2778447006","wikidata":"https://www.wikidata.org/wiki/Q1156474","display_name":"Icon","level":2,"score":0.4232657253742218},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.3435520529747009},{"id":"https://openalex.org/C136764020","wikidata":"https://www.wikidata.org/wiki/Q466","display_name":"World Wide Web","level":1,"score":0.2882764935493469},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.2180441915988922},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.15699061751365662},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007962","descriptor_name":"Leukocytes","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010179","descriptor_name":"Pancreas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010196","descriptor_name":"Pancreatic Elastase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D010196","descriptor_name":"Pancreatic Elastase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011480","descriptor_name":"Protease Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014961","descriptor_name":"X-Ray Diffraction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00431a001","doi":"https://doi.org/10.1021/bi00431a001","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00431a001","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:2655701","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2655701","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5349375883","display_name":"SYNTHETIC ELASTASE INHIBITORS","funder_award_id":"R01HL029307","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G6092953265","display_name":"SYNTHETIC ANTITHROMBOTIC AGENTS","funder_award_id":"R01HL034035","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320310010","display_name":"University of Georgia","ror":"https://ror.org/00te3t702"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":81,"referenced_works":["https://openalex.org/W56641540","https://openalex.org/W153791503","https://openalex.org/W173669577","https://openalex.org/W1483877097","https://openalex.org/W1494805566","https://openalex.org/W1496675280","https://openalex.org/W1498321805","https://openalex.org/W1535477876","https://openalex.org/W1539490926","https://openalex.org/W1539493560","https://openalex.org/W1584449567","https://openalex.org/W1587501715","https://openalex.org/W1595615359","https://openalex.org/W1597669312","https://openalex.org/W1754330606","https://openalex.org/W1823385938","https://openalex.org/W1964543463","https://openalex.org/W1965451226","https://openalex.org/W1969629201","https://openalex.org/W1974341619","https://openalex.org/W1979133192","https://openalex.org/W1981973514","https://openalex.org/W1983498279","https://openalex.org/W1985469117","https://openalex.org/W1986828098","https://openalex.org/W1987470484","https://openalex.org/W1988052822","https://openalex.org/W1988290362","https://openalex.org/W1988476878","https://openalex.org/W1989413245","https://openalex.org/W1992080559","https://openalex.org/W1996697803","https://openalex.org/W1997438880","https://openalex.org/W2007145119","https://openalex.org/W2010986740","https://openalex.org/W2012384963","https://openalex.org/W2015470620","https://openalex.org/W2019024904","https://openalex.org/W2019584004","https://openalex.org/W2020786020","https://openalex.org/W2022416858","https://openalex.org/W2024882709","https://openalex.org/W2025130580","https://openalex.org/W2030461289","https://openalex.org/W2037082246","https://openalex.org/W2037294611","https://openalex.org/W2040180992","https://openalex.org/W2040777172","https://openalex.org/W2042324648","https://openalex.org/W2043799766","https://openalex.org/W2045618499","https://openalex.org/W2045734210","https://openalex.org/W2046015446","https://openalex.org/W2047314425","https://openalex.org/W2049357506","https://openalex.org/W2051462955","https://openalex.org/W2057307013","https://openalex.org/W2058798515","https://openalex.org/W2067120533","https://openalex.org/W2069718808","https://openalex.org/W2071030556","https://openalex.org/W2072860427","https://openalex.org/W2082230373","https://openalex.org/W2084122119","https://openalex.org/W2087883017","https://openalex.org/W2089824534","https://openalex.org/W2106593228","https://openalex.org/W2107873069","https://openalex.org/W2112636022","https://openalex.org/W2113257020","https://openalex.org/W2114621223","https://openalex.org/W2119128997","https://openalex.org/W2140422547","https://openalex.org/W2144221768","https://openalex.org/W2149134776","https://openalex.org/W2156324634","https://openalex.org/W2161189887","https://openalex.org/W2198846731","https://openalex.org/W2319443743","https://openalex.org/W2342945340","https://openalex.org/W3004581983"],"related_works":["https://openalex.org/W2010974527","https://openalex.org/W2046015446","https://openalex.org/W1627442108","https://openalex.org/W1967719524","https://openalex.org/W7098306579","https://openalex.org/W2611151791","https://openalex.org/W2978604862","https://openalex.org/W2077161860","https://openalex.org/W1531186053","https://openalex.org/W2057146216"],"abstract_inverted_index":{"ADVERTISEMENT":[0],"RETURN":[1],"TO":[2],"ISSUEPREVArticleNEXTHuman":[3],"leukocyte":[4],"and":[5,15,22,67,72,101,145,162,178,180],"porcine":[6],"pancreatic":[7],"elastase:":[8],"x-ray":[9],"crystal":[10],"structures,":[11],"mechanism,":[12],"substrate":[13],"specificity,":[14],"mechanism-based":[16],"inhibitorsWolfram":[17],"Bode,":[18],"Edgar":[19],"Meyer,":[20],"Jr.,":[21],"James":[23],"C.":[24],"PowersCite":[25],"this:":[26],"Biochemistry":[27],"1989,":[28],"28,":[29],"5,":[30],"1951\u20131963Publication":[31],"Date":[32],"(Print):March":[33],"7,":[34],"1989Publication":[35],"History":[36],"Published":[37],"online1":[38],"May":[39],"2002Published":[40],"inissue":[41],"7":[42],"March":[43],"1989https://pubs.acs.org/doi/10.1021/bi00431a001https://doi.org/10.1021/bi00431a001research-articleACS":[44],"PublicationsRequest":[45],"reuse":[46],"permissionsArticle":[47],"Views1200Altmetric-Citations302LEARN":[48],"ABOUT":[49],"THESE":[50],"METRICSArticle":[51],"Views":[52],"are":[53,76,89],"the":[54,85,90,119,130,143,146,151,158,164],"COUNTER-compliant":[55],"sum":[56],"of":[57,92,118],"full":[58],"text":[59],"article":[60,124],"downloads":[61],"since":[62],"November":[63],"2008":[64],"(both":[65],"PDF":[66],"HTML)":[68],"across":[69],"all":[70],"institutions":[71],"individuals.":[73],"These":[74],"metrics":[75],"regularly":[77],"updated":[78,102],"to":[79,84],"reflect":[80],"usage":[81],"leading":[82],"up":[83],"last":[86],"few":[87],"days.Citations":[88],"number":[91],"other":[93],"articles":[94],"citing":[95],"this":[96],"article,":[97],"calculated":[98],"by":[99],"Crossref":[100,108],"daily.":[103],"Find":[104,154],"more":[105,155],"information":[106,156],"about":[107,142],"citation":[109],"counts.The":[110],"Altmetric":[111,159],"Attention":[112,160],"Score":[113,161],"is":[114,166],"a":[115,122,135],"quantitative":[116],"measure":[117],"attention":[120],"that":[121],"research":[123],"has":[125],"received":[126],"online.":[127],"Clicking":[128],"on":[129,157],"donut":[131],"icon":[132],"will":[133],"load":[134],"page":[136],"at":[137],"altmetric.com":[138],"with":[139,174],"additional":[140],"details":[141],"score":[144,165],"social":[147],"media":[148],"presence":[149],"for":[150],"given":[152],"article.":[153],"how":[163],"calculated.":[167],"Share":[168,183],"Add":[169],"toView":[170],"InAdd":[171],"Full":[172],"Text":[173],"ReferenceAdd":[175],"Description":[176],"ExportRISCitationCitation":[177],"abstractCitation":[179],"referencesMore":[181],"Options":[182],"onFacebookTwitterWechatLinked":[184],"InRedditEmail":[185],"Other":[186],"access":[187],"optionsGet":[188],"e-Alertsclose":[189],"Get":[190],"e-Alerts":[191]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":11},{"year":2014,"cited_by_count":8},{"year":2013,"cited_by_count":8},{"year":2012,"cited_by_count":14}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}