{"id":"https://openalex.org/W1995449819","doi":"https://doi.org/10.3791/4282","title":"Bacterial Detection & Identification Using Electrochemical Sensors","display_name":"Bacterial Detection & Identification Using Electrochemical Sensors","publication_year":2013,"publication_date":"2013-04-23","ids":{"openalex":"https://openalex.org/W1995449819","doi":"https://doi.org/10.3791/4282","mag":"1995449819","pmid":"https://pubmed.ncbi.nlm.nih.gov/23644406","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3667549"},"language":"en","primary_location":{"id":"doi:10.3791/4282","doi":"https://doi.org/10.3791/4282","is_oa":true,"landing_page_url":"https://doi.org/10.3791/4282","pdf_url":"https://www.jove.com/pdf/4282/bacterial-detection-identification-using-electrochemical-sensors","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jove.com/pdf/4282/bacterial-detection-identification-using-electrochemical-sensors","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010948579","display_name":"Colin Halford","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]},{"id":"https://openalex.org/I4210156876","display_name":"VA Greater Los Angeles Healthcare System","ror":"https://ror.org/05xcarb80","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I4210138663","https://openalex.org/I4210156876"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Colin Halford","raw_affiliation_strings":["Research Service, Veterans Affairs Greater Los Angeles Healthcare System","Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Service, Veterans Affairs Greater Los Angeles Healthcare System","institution_ids":["https://openalex.org/I4210156876"]},{"raw_affiliation_string":"Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036953923","display_name":"Vincent Gau","orcid":"https://orcid.org/0000-0002-1948-0310","observed_orcids":["https://orcid.org/0000-0002-1948-0310"]},"institutions":[{"id":"https://openalex.org/I4210155608","display_name":"GeneFluidics (United States)","ror":"https://ror.org/04dv5rj21","country_code":"US","type":"company","lineage":["https://openalex.org/I4210155608"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vincent Gau","raw_affiliation_strings":["GeneFluidics"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"GeneFluidics","institution_ids":["https://openalex.org/I4210155608"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112171707","display_name":"Bernard M. Churchill","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bernard M. Churchill","raw_affiliation_strings":["Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5053911927","display_name":"David A. Haake","orcid":"https://orcid.org/0000-0002-8468-6526","observed_orcids":["https://orcid.org/0000-0002-8468-6526"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]},{"id":"https://openalex.org/I4210156876","display_name":"VA Greater Los Angeles Healthcare System","ror":"https://ror.org/05xcarb80","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I4210138663","https://openalex.org/I4210156876"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David A. Haake","raw_affiliation_strings":["Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles","Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles","Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles","institution_ids":["https://openalex.org/I161318765"]},{"raw_affiliation_string":"Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles","institution_ids":["https://openalex.org/I161318765"]},{"raw_affiliation_string":"Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System","institution_ids":["https://openalex.org/I4210156876"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1997,"has_fulltext":true,"cited_by_count":7,"citation_normalized_percentile":{"value":0.6101138,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":null,"issue":"74","first_page":"e4282","last_page":"e4282"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10212","display_name":"Electrochemical sensors and biosensors","score":0.9185000061988831,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10212","display_name":"Electrochemical sensors and biosensors","score":0.9185000061988831,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.07810000330209732,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/electrochemical-sensor","display_name":"electrochemical sensor","score":0.777999997138977},{"id":"https://openalex.org/keywords/bacterial-detection","display_name":"bacterial detection","score":0.9810000061988831},{"id":"https://openalex.org/keywords/bacterial-identification","display_name":"bacterial identification","score":0.9599999785423279},{"id":"https://openalex.org/keywords/dna-probe-hybridization","display_name":"DNA probe hybridization","score":0.593999981880188},{"id":"https://openalex.org/keywords/sandwich-hybridization","display_name":"sandwich hybridization","score":0.9330000281333923},{"id":"https://openalex.org/keywords/ribosomal-rna","display_name":"ribosomal RNA","score":0.9290000200271606},{"id":"https://openalex.org/keywords/horseradish-peroxidase","display_name":"horseradish peroxidase","score":0.9860000014305115},{"id":"https://openalex.org/keywords/tetramethylbenzidine","display_name":"tetramethylbenzidine","score":0.878000020980835},{"id":"https://openalex.org/keywords/amperometric-detection","display_name":"amperometric detection","score":0.7459999918937683}],"concepts":[{"id":"https://openalex.org/C17525397","wikidata":"https://www.wikidata.org/wiki/Q176140","display_name":"Electrode","level":2,"score":0.6082175374031067},{"id":"https://openalex.org/C160756335","wikidata":"https://www.wikidata.org/wiki/Q669391","display_name":"Biosensor","level":2,"score":0.5971248149871826},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.5737273693084717},{"id":"https://openalex.org/C139369640","wikidata":"https://www.wikidata.org/wiki/Q474536","display_name":"Amperometry","level":4,"score":0.5376983284950256},{"id":"https://openalex.org/C2777676563","wikidata":"https://www.wikidata.org/wiki/Q413665","display_name":"Horseradish peroxidase","level":3,"score":0.5338097214698792},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4710927903652191},{"id":"https://openalex.org/C111824103","wikidata":"https://www.wikidata.org/wiki/Q7086828","display_name":"Oligomer restriction","level":4,"score":0.467040479183197},{"id":"https://openalex.org/C130197536","wikidata":"https://www.wikidata.org/wiki/Q418436","display_name":"Hybridization probe","level":3,"score":0.44992977380752563},{"id":"https://openalex.org/C69118416","wikidata":"https://www.wikidata.org/wiki/Q477099","display_name":"Working electrode","level":4,"score":0.42502179741859436},{"id":"https://openalex.org/C2777197210","wikidata":"https://www.wikidata.org/wiki/Q419321","display_name":"Glucose oxidase","level":3,"score":0.4238632023334503},{"id":"https://openalex.org/C188095982","wikidata":"https://www.wikidata.org/wiki/Q5357970","display_name":"Electrochemical gas sensor","level":4,"score":0.4118649363517761},{"id":"https://openalex.org/C52859227","wikidata":"https://www.wikidata.org/wiki/Q7877","display_name":"Electrochemistry","level":3,"score":0.3953911066055298},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.33390718698501587},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2917754054069519},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.17096492648124695},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.14467483758926392},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12827879190444946},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":true},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D001431","descriptor_name":"Bacteriological Techniques","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":true},{"descriptor_ui":"D001431","descriptor_name":"Bacteriological Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D012335","descriptor_name":"RNA, Ribosomal","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012335","descriptor_name":"RNA, Ribosomal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D055664","descriptor_name":"Electrochemical Techniques","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":true},{"descriptor_ui":"D055664","descriptor_name":"Electrochemical Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.3791/4282","doi":"https://doi.org/10.3791/4282","is_oa":true,"landing_page_url":"https://doi.org/10.3791/4282","pdf_url":"https://www.jove.com/pdf/4282/bacterial-detection-identification-using-electrochemical-sensors","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3667549","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3667549","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Vis Exp","raw_type":"Text"},{"id":"pmid:23644406","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23644406","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.3791/4282","doi":"https://doi.org/10.3791/4282","is_oa":true,"landing_page_url":"https://doi.org/10.3791/4282","pdf_url":"https://www.jove.com/pdf/4282/bacterial-detection-identification-using-electrochemical-sensors","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5110902367","display_name":null,"funder_award_id":"AI075565","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G8361022650","display_name":"Sensor Technology for Rapid Microbial Identification and Susceptibility Testing","funder_award_id":"U01AI075565","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1995449819.pdf","grobid_xml":"https://content.openalex.org/works/W1995449819.grobid-xml"},"referenced_works_count":12,"referenced_works":["https://openalex.org/W1964731949","https://openalex.org/W1986660497","https://openalex.org/W2043487612","https://openalex.org/W2048283640","https://openalex.org/W2060857484","https://openalex.org/W2075223225","https://openalex.org/W2101099832","https://openalex.org/W2125434152","https://openalex.org/W2131630815","https://openalex.org/W2136569648","https://openalex.org/W2149394003","https://openalex.org/W2326917604"],"related_works":["https://openalex.org/W2043487612","https://openalex.org/W2506428668","https://openalex.org/W2952820816","https://openalex.org/W4384518902","https://openalex.org/W4295807918","https://openalex.org/W3212568891","https://openalex.org/W2038654759","https://openalex.org/W1971279231","https://openalex.org/W2495528714","https://openalex.org/W2160535599"],"abstract_inverted_index":{"Electrochemical":[0,25],"sensors":[1,26],"are":[2,107],"widely":[3],"used":[4],"for":[5,17],"rapid":[6],"and":[7,13,81,97,119,171],"accurate":[8],"measurement":[9],"of":[10,19,23,46,61,69,76,117,183],"blood":[11],"glucose":[12,64,71],"can":[14],"be":[15],"adapted":[16],"detection":[18],"a":[20,30,35,47,70,74,147],"wide":[21],"variety":[22],"analytes.":[24],"operate":[27],"by":[28,42,169,173,185],"transducing":[29],"biological":[31],"recognition":[32],"event":[33],"into":[34],"useful":[36],"electrical":[37],"signal.":[38],"Signal":[39],"transduction":[40],"occurs":[41],"coupling":[43],"the":[44,62,67,79,103,133,137,165,174,186,189,197],"activity":[45],"redox":[48,178],"enzyme":[49,80],"to":[50,94,132,142,155,162,191],"an":[51,58,82,89,156],"amperometric":[52],"electrode.":[53,176,198],"Sensor":[54],"specificity":[55],"is":[56,112,130,140,153,167],"either":[57],"inherent":[59],"characteristic":[60],"enzyme,":[63],"oxidase":[65],"in":[66,181,196],"case":[68],"sensor,":[72],"or":[73,84],"product":[75],"linkage":[77],"between":[78],"antibody":[83],"probe.":[85],"Here,":[86],"we":[87],"describe":[88],"electrochemical":[90],"sensor":[91,134],"assay":[92],"method":[93,111],"directly":[95],"detect":[96],"identify":[98],"bacteria.":[99],"In":[100],"every":[101],"case,":[102],"probes":[104,121],"described":[105],"here":[106],"DNA":[108],"oligonucleotides.":[109],"This":[110,177],"based":[113],"on":[114],"sandwich":[115],"hybridization":[116],"capture":[118,128],"detector":[120,138],"with":[122,158],"target":[123],"ribosomal":[124],"RNA":[125],"(rRNA).":[126],"The":[127],"probe":[129,139],"anchored":[131],"surface,":[135,164],"while":[136],"linked":[141],"horseradish":[143],"peroxidase":[144],"(HRP).":[145],"When":[146],"substrate":[148,166,187],"such":[149],"as":[150],"3,3',5,5'-tetramethylbenzidine":[151],"(TMB)":[152],"added":[154],"electrode":[157,190],"capture-target-detector":[159],"complexes":[160],"bound":[161],"its":[163],"oxidized":[168],"HRP":[170],"reduced":[172],"working":[175],"cycle":[179],"results":[180],"shuttling":[182],"electrons":[184],"from":[188],"HRP,":[192],"producing":[193],"current":[194],"flow":[195]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}