{"id":"https://openalex.org/W1995235658","doi":"https://doi.org/10.1021/ja011853+","title":"Mechanism of Reaction of Hydrogen Peroxide with Horseradish Peroxidase: Identification of Intermediates in the Catalytic Cycle","display_name":"Mechanism of Reaction of Hydrogen Peroxide with Horseradish Peroxidase: Identification of Intermediates in the Catalytic Cycle","publication_year":2001,"publication_date":"2001-11-01","ids":{"openalex":"https://openalex.org/W1995235658","doi":"https://doi.org/10.1021/ja011853+","mag":"1995235658","pmid":"https://pubmed.ncbi.nlm.nih.gov/11724589"},"language":"en","primary_location":{"id":"doi:10.1021/ja011853+","doi":"https://doi.org/10.1021/ja011853+","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja011853+","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086418754","display_name":"Jos\u00e9 Neptuno Rodr\u00edguez\u2010L\u00f3pez","orcid":"https://orcid.org/0000-0001-6863-1173","observed_orcids":["https://orcid.org/0000-0001-6863-1173"]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I2799300731","display_name":"Norwich Research Park","ror":"https://ror.org/0062dz060","country_code":"GB","type":"archive","lineage":["https://openalex.org/I2799300731"]},{"id":"https://openalex.org/I80180929","display_name":"Universidad de Murcia","ror":"https://ror.org/03p3aeb86","country_code":"ES","type":"education","lineage":["https://openalex.org/I80180929"]}],"countries":["ES","GB"],"is_corresponding":false,"raw_author_name":"Jose Neptuno Rodr\u00edguez-L\u00f3pez","raw_affiliation_strings":["Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom","institution_ids":["https://openalex.org/I100288624","https://openalex.org/I2799300731","https://openalex.org/I80180929"]}]},{"author_position":"middle","author":{"id":null,"display_name":"David J. Lowe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I2799300731","display_name":"Norwich Research Park","ror":"https://ror.org/0062dz060","country_code":"GB","type":"archive","lineage":["https://openalex.org/I2799300731"]},{"id":"https://openalex.org/I80180929","display_name":"Universidad de Murcia","ror":"https://ror.org/03p3aeb86","country_code":"ES","type":"education","lineage":["https://openalex.org/I80180929"]}],"countries":["ES","GB"],"is_corresponding":false,"raw_author_name":"David J. Lowe","raw_affiliation_strings":["Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom","institution_ids":["https://openalex.org/I100288624","https://openalex.org/I2799300731","https://openalex.org/I80180929"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064899538","display_name":"Josefa Hern\u00e1ndez\u2010Ru\u00edz","orcid":"https://orcid.org/0000-0002-1847-8809","observed_orcids":["https://orcid.org/0000-0002-1847-8809"]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I2799300731","display_name":"Norwich Research Park","ror":"https://ror.org/0062dz060","country_code":"GB","type":"archive","lineage":["https://openalex.org/I2799300731"]},{"id":"https://openalex.org/I80180929","display_name":"Universidad de Murcia","ror":"https://ror.org/03p3aeb86","country_code":"ES","type":"education","lineage":["https://openalex.org/I80180929"]}],"countries":["ES","GB"],"is_corresponding":false,"raw_author_name":"Josefa Hern\u00e1ndez-Ruiz","raw_affiliation_strings":["Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom","institution_ids":["https://openalex.org/I100288624","https://openalex.org/I2799300731","https://openalex.org/I80180929"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013091514","display_name":"Alexander N. P. Hiner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I2799300731","display_name":"Norwich Research Park","ror":"https://ror.org/0062dz060","country_code":"GB","type":"archive","lineage":["https://openalex.org/I2799300731"]},{"id":"https://openalex.org/I80180929","display_name":"Universidad de Murcia","ror":"https://ror.org/03p3aeb86","country_code":"ES","type":"education","lineage":["https://openalex.org/I80180929"]}],"countries":["ES","GB"],"is_corresponding":false,"raw_author_name":"Alexander N. P. Hiner","raw_affiliation_strings":["Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom","institution_ids":["https://openalex.org/I100288624","https://openalex.org/I2799300731","https://openalex.org/I80180929"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035002495","display_name":"Francisco Garc\u0131\u0301a-C\u00e1novas","orcid":"https://orcid.org/0000-0002-8869-067X","observed_orcids":["https://orcid.org/0000-0002-8869-067X"]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I2799300731","display_name":"Norwich Research Park","ror":"https://ror.org/0062dz060","country_code":"GB","type":"archive","lineage":["https://openalex.org/I2799300731"]},{"id":"https://openalex.org/I80180929","display_name":"Universidad de Murcia","ror":"https://ror.org/03p3aeb86","country_code":"ES","type":"education","lineage":["https://openalex.org/I80180929"]}],"countries":["ES","GB"],"is_corresponding":false,"raw_author_name":"Francisco Garc\u00eda-C\u00e1novas","raw_affiliation_strings":["Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom","institution_ids":["https://openalex.org/I100288624","https://openalex.org/I2799300731","https://openalex.org/I80180929"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109054939","display_name":"Roger N. F. THORNELEY","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100288624","display_name":"John Innes Centre","ror":"https://ror.org/055zmrh94","country_code":"GB","type":"facility","lineage":["https://openalex.org/I100288624","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I2799300731","display_name":"Norwich Research Park","ror":"https://ror.org/0062dz060","country_code":"GB","type":"archive","lineage":["https://openalex.org/I2799300731"]},{"id":"https://openalex.org/I80180929","display_name":"Universidad de Murcia","ror":"https://ror.org/03p3aeb86","country_code":"ES","type":"education","lineage":["https://openalex.org/I80180929"]}],"countries":["ES","GB"],"is_corresponding":true,"raw_author_name":"Roger N. F. Thorneley","raw_affiliation_strings":["Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular A and Departamento de Biolog\u00eda Vegetal, Facultad de Biolog\u00eda, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain, and Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom","institution_ids":["https://openalex.org/I100288624","https://openalex.org/I2799300731","https://openalex.org/I80180929"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5109054939"],"corresponding_institution_ids":["https://openalex.org/I100288624","https://openalex.org/I2799300731","https://openalex.org/I80180929"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":6.7018,"has_fulltext":false,"cited_by_count":348,"citation_normalized_percentile":{"value":0.98337708,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"123","issue":"48","first_page":"11838","last_page":"11847"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Physical Organic Chemistry and Reaction Kinetics","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12094","display_name":"Hemoglobin structure and function","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/horseradish-peroxidase-c","display_name":"horseradish peroxidase C","score":0.902999997138977},{"id":"https://openalex.org/keywords/hydrogen-peroxide","display_name":"hydrogen peroxide","score":0.8949999809265137},{"id":"https://openalex.org/keywords/compound-i","display_name":"compound I","score":0.8889999985694885},{"id":"https://openalex.org/keywords/enzyme-mechanism","display_name":"enzyme mechanism","score":0.6340000033378601},{"id":"https://openalex.org/keywords/heterolytic-cleavage","display_name":"heterolytic cleavage","score":0.8109999895095825},{"id":"https://openalex.org/keywords/homolytic-cleavage","display_name":"homolytic cleavage","score":0.9459999799728394},{"id":"https://openalex.org/keywords/active-site-residues","display_name":"active site residues","score":0.7059999704360962},{"id":"https://openalex.org/keywords/site-directed-mutants","display_name":"site-directed mutants","score":0.6150000095367432},{"id":"https://openalex.org/keywords/ph-dependence","display_name":"pH dependence","score":0.48100000619888306},{"id":"https://openalex.org/keywords/pka","display_name":"pKa","score":0.4480000138282776},{"id":"https://openalex.org/keywords/protein-radicals","display_name":"protein radicals","score":0.5920000076293945},{"id":"https://openalex.org/keywords/abts","display_name":"ABTS","score":0.40299999713897705},{"id":"https://openalex.org/keywords/reaction-intermediates","display_name":"reaction intermediates","score":0.5550000071525574},{"id":"https://openalex.org/keywords/heme-peroxidase","display_name":"heme peroxidase","score":0.49799999594688416}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9717617034912109},{"id":"https://openalex.org/C2777676563","wikidata":"https://www.wikidata.org/wiki/Q413665","display_name":"Horseradish peroxidase","level":3,"score":0.7848583459854126},{"id":"https://openalex.org/C44578547","wikidata":"https://www.wikidata.org/wiki/Q907567","display_name":"Heterolysis","level":3,"score":0.7035055160522461},{"id":"https://openalex.org/C533411734","wikidata":"https://www.wikidata.org/wiki/Q171877","display_name":"Hydrogen peroxide","level":2,"score":0.6829178333282471},{"id":"https://openalex.org/C175689099","wikidata":"https://www.wikidata.org/wiki/Q908109","display_name":"Homolysis","level":3,"score":0.587354302406311},{"id":"https://openalex.org/C187961010","wikidata":"https://www.wikidata.org/wiki/Q260463","display_name":"Electron paramagnetic resonance","level":2,"score":0.5780011415481567},{"id":"https://openalex.org/C162008176","wikidata":"https://www.wikidata.org/wiki/Q419305","display_name":"Peroxidase","level":3,"score":0.5422760248184204},{"id":"https://openalex.org/C75473681","wikidata":"https://www.wikidata.org/wiki/Q188651","display_name":"Photochemistry","level":1,"score":0.5344642400741577},{"id":"https://openalex.org/C105768512","wikidata":"https://www.wikidata.org/wiki/Q7458208","display_name":"Reaction intermediate","level":3,"score":0.5322052836418152},{"id":"https://openalex.org/C63338738","wikidata":"https://www.wikidata.org/wiki/Q287862","display_name":"Catalytic cycle","level":3,"score":0.5039872527122498},{"id":"https://openalex.org/C155647269","wikidata":"https://www.wikidata.org/wiki/Q243455","display_name":"Medicinal chemistry","level":1,"score":0.48629483580589294},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.4805596172809601},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.45964810252189636},{"id":"https://openalex.org/C2781048764","wikidata":"https://www.wikidata.org/wiki/Q107429","display_name":"Peroxide","level":2,"score":0.450699120759964},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.45040470361709595},{"id":"https://openalex.org/C3044715","wikidata":"https://www.wikidata.org/wiki/Q3952437","display_name":"Bond cleavage","level":3,"score":0.4400976896286011},{"id":"https://openalex.org/C2776217839","wikidata":"https://www.wikidata.org/wiki/Q189621","display_name":"Heme","level":3,"score":0.4156700074672699},{"id":"https://openalex.org/C65024703","wikidata":"https://www.wikidata.org/wiki/Q373406","display_name":"Reaction mechanism","level":3,"score":0.41376304626464844},{"id":"https://openalex.org/C139066938","wikidata":"https://www.wikidata.org/wiki/Q185056","display_name":"Radical","level":2,"score":0.40609192848205566},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.24171271920204163},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.21031039953231812},{"id":"https://openalex.org/C46141821","wikidata":"https://www.wikidata.org/wiki/Q209402","display_name":"Nuclear magnetic resonance","level":1,"score":0.07836663722991943},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004578","descriptor_name":"Electron Spin Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006735","descriptor_name":"Horseradish Peroxidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006735","descriptor_name":"Horseradish Peroxidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006735","descriptor_name":"Horseradish Peroxidase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006861","descriptor_name":"Hydrogen Peroxide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006861","descriptor_name":"Hydrogen Peroxide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/ja011853+","doi":"https://doi.org/10.1021/ja011853+","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja011853+","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"pmid:11724589","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11724589","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W349592250","https://openalex.org/W1565691495","https://openalex.org/W1571455001","https://openalex.org/W1968130661","https://openalex.org/W1970034308","https://openalex.org/W1972364431","https://openalex.org/W1972943879","https://openalex.org/W1981743307","https://openalex.org/W1982706580","https://openalex.org/W2002978594","https://openalex.org/W2005777794","https://openalex.org/W2009840004","https://openalex.org/W2010454869","https://openalex.org/W2015523027","https://openalex.org/W2016573311","https://openalex.org/W2023042894","https://openalex.org/W2040883347","https://openalex.org/W2048459982","https://openalex.org/W2053196170","https://openalex.org/W2057136700","https://openalex.org/W2073234132","https://openalex.org/W2075766195","https://openalex.org/W2079390178","https://openalex.org/W2085266874","https://openalex.org/W2088359937","https://openalex.org/W2090810684","https://openalex.org/W2092643557","https://openalex.org/W2095873332","https://openalex.org/W2115350007","https://openalex.org/W2505584743","https://openalex.org/W2949521180","https://openalex.org/W2951403792"],"related_works":["https://openalex.org/W2079390178","https://openalex.org/W1969104821","https://openalex.org/W2017540300","https://openalex.org/W1583051569","https://openalex.org/W2284208289","https://openalex.org/W2048416341","https://openalex.org/W2092655611","https://openalex.org/W2100600786","https://openalex.org/W2035352601","https://openalex.org/W2072590806"],"abstract_inverted_index":{"The":[0,42,81,135,177],"mechanism":[1],"of":[2,5,44,56,85,92,121,137,140,157,180,206,234,269,283],"the":[3,16,45,57,90,112,119,132,138,141,197,204,218,262,281,291],"reaction":[4,83,139,143],"horseradish":[6],"peroxidase":[7],"isoenzyme":[8],"C":[9],"(HRPC)":[10],"with":[11,64,87,210],"hydrogen":[12],"peroxide":[13],"to":[14,106,159,193,223],"form":[15],"reactive":[17],"enzyme":[18],"intermediate":[19,94],"compound":[20,114,181],"I":[21,182],"has":[22],"been":[23,62],"studied":[24],"using":[25],"electronic":[26],"absorbance,":[27],"rapid-scan":[28],"stopped-flow,":[29],"and":[30,39,50,76,111,145,152,216,250,260,266,285],"electron":[31],"paramagnetic":[32],"resonance":[33],"(EPR)":[34],"spectroscopies":[35],"at":[36,97,200,257,273],"both":[37],"acid":[38,98,201,274],"basic":[40],"pH.":[41,275],"roles":[43],"active":[46,292],"site":[47],"residues":[48],"His42":[49,68,241,284],"Arg38":[51,72,77,276],"in":[52,175,191,279,287,290,302],"controlling":[53],"heterolytic":[54,270],"cleavage":[55,174,233,271],"H(2)O(2)":[58,88,171,235,289],"oxygen-oxygen":[59],"bond":[60],"have":[61],"probed":[63],"site-directed":[65],"mutant":[66],"enzymes":[67],"-->":[69,73,78],"Leu":[70,74],"(H42L),":[71],"(R38L),":[75],"Gly":[79],"(R38G).":[80],"biphasic":[82],"kinetics":[84],"H42L":[86],"suggested":[89],"presence":[91],"an":[93,207],"species":[95],"and,":[96],"pH,":[99,202],"a":[100,107,122,155,245,253,299],"reversible":[101],"second":[102],"step,":[103],"probably":[104],"due":[105],"neutral":[108,258],"enzyme-H(2)O(2)":[109],"complex":[110],"ferric-peroxoanion-containing":[113],"0.":[115],"EPR":[116],"also":[117],"indicated":[118],"formation":[120,183],"protein":[123],"radical":[124],"situated":[125],"more":[126],"than":[127],"approximately":[128,212,224],"10":[129],"A":[130],"from":[131,154],"heme":[133],"iron.":[134],"stoichiometry":[136],"H42L/H(2)O(2)":[142],"product":[144],"2,2'-azinobis(3-ethylbenzothiazolinesulfonic":[146],"acid)":[147],"(ABTS)":[148],"was":[149,188,221],"concentration":[150],"dependent":[151],"fell":[153],"value":[156],"2":[158],"1":[160],"above":[161],"0.7":[162],"mM":[163],"ABTS.":[164],"These":[165,237],"data":[166,238],"can":[167],"be":[168],"explained":[169],"if":[170],"undergoes":[172],"homolytic":[173,232],"H42L.":[176],"apparent":[178,219],"rate":[179,198,265],"by":[184],"H42L,":[185],"while":[186],"low,":[187],"pH":[189,259],"independent":[190],"contrast":[192],"wild-type":[194],"HRPC":[195],"where":[196],"falls":[199],"indicating":[203],"involvement":[205],"ionizable":[208],"group":[209],"pK(a)":[211,220,282],"4.":[213],"In":[214],"R38L":[215],"R38G,":[217],"shifted":[222],"8":[225],"but":[226,294],"there":[227],"is":[228,277],"no":[229],"evidence":[230],"that":[231,240],"occurs.":[236],"suggest":[239],"acts":[242],"initially":[243],"as":[244,252],"proton":[246,303],"acceptor":[247],"(base":[248],"catalyst)":[249,256],"then":[251],"donor":[254],"(acid":[255],"predict":[261],"observed":[263,272],"slower":[264],"lower":[267],"efficiency":[268],"influential":[278],"lowering":[280],"additionally":[286],"aligning":[288],"site,":[293],"it":[295],"does":[296],"not":[297],"play":[298],"direct":[300],"role":[301],"transfer.":[304]},"counts_by_year":[{"year":2026,"cited_by_count":17},{"year":2025,"cited_by_count":20},{"year":2024,"cited_by_count":16},{"year":2023,"cited_by_count":13},{"year":2022,"cited_by_count":22},{"year":2021,"cited_by_count":21},{"year":2020,"cited_by_count":35},{"year":2019,"cited_by_count":18},{"year":2018,"cited_by_count":18},{"year":2017,"cited_by_count":13},{"year":2016,"cited_by_count":11},{"year":2015,"cited_by_count":15},{"year":2014,"cited_by_count":11},{"year":2013,"cited_by_count":13},{"year":2012,"cited_by_count":13}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}