{"id":"https://openalex.org/W1995160660","doi":"https://doi.org/10.1074/jbc.273.39.25244","title":"Human \u03b11,3/4-Fucosyltransferases","display_name":"Human \u03b11,3/4-Fucosyltransferases","publication_year":1998,"publication_date":"1998-09-01","ids":{"openalex":"https://openalex.org/W1995160660","doi":"https://doi.org/10.1074/jbc.273.39.25244","mag":"1995160660","pmid":"https://pubmed.ncbi.nlm.nih.gov/9737988"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.273.39.25244","doi":"https://doi.org/10.1074/jbc.273.39.25244","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.39.25244","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.273.39.25244","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030093070","display_name":"Anton Nguyen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71838634","display_name":"San Francisco State University","ror":"https://ror.org/05ykr0121","country_code":"US","type":"education","lineage":["https://openalex.org/I71838634"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anton T. Nguyen","raw_affiliation_strings":["Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132, USA","institution_ids":["https://openalex.org/I71838634"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111754017","display_name":"Eric H. Holmes","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71838634","display_name":"San Francisco State University","ror":"https://ror.org/05ykr0121","country_code":"US","type":"education","lineage":["https://openalex.org/I71838634"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Eric H. Holmes","raw_affiliation_strings":["Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125","From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125","institution_ids":[]},{"raw_affiliation_string":"From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#","institution_ids":["https://openalex.org/I71838634"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110531658","display_name":"Jeffrey M. Whitaker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71838634","display_name":"San Francisco State University","ror":"https://ror.org/05ykr0121","country_code":"US","type":"education","lineage":["https://openalex.org/I71838634"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffrey M. Whitaker","raw_affiliation_strings":["Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132","From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132","institution_ids":["https://openalex.org/I71838634"]},{"raw_affiliation_string":"From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#","institution_ids":["https://openalex.org/I71838634"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071689619","display_name":"Stephanie Ho","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71838634","display_name":"San Francisco State University","ror":"https://ror.org/05ykr0121","country_code":"US","type":"education","lineage":["https://openalex.org/I71838634"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephanie Ho","raw_affiliation_strings":["Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132","From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132","institution_ids":["https://openalex.org/I71838634"]},{"raw_affiliation_string":"From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#","institution_ids":["https://openalex.org/I71838634"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Susan Shetterly","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71838634","display_name":"San Francisco State University","ror":"https://ror.org/05ykr0121","country_code":"US","type":"education","lineage":["https://openalex.org/I71838634"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan Shetterly","raw_affiliation_strings":["Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132","From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132","institution_ids":["https://openalex.org/I71838634"]},{"raw_affiliation_string":"From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#","institution_ids":["https://openalex.org/I71838634"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110076366","display_name":"Bruce A. Macher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I71838634","display_name":"San Francisco State University","ror":"https://ror.org/05ykr0121","country_code":"US","type":"education","lineage":["https://openalex.org/I71838634"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bruce A. Macher","raw_affiliation_strings":["Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132","From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132","institution_ids":["https://openalex.org/I71838634"]},{"raw_affiliation_string":"From the #N#\u2021Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, California 94132 and#N##N#\u00a7Northwest Hospital, Pacific Northwest Cancer Foundation, Seattle, Washington 98125#N#","institution_ids":["https://openalex.org/I71838634"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5111754017"],"corresponding_institution_ids":["https://openalex.org/I71838634"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.3131,"has_fulltext":false,"cited_by_count":50,"citation_normalized_percentile":{"value":0.88751377,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"273","issue":"39","first_page":"25244","last_page":"25249"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11660","display_name":"Blood groups and transfusion","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acceptor-substrate-specificity","display_name":"acceptor substrate specificity","score":0.8220000267028809},{"id":"https://openalex.org/keywords/domain-swapping","display_name":"domain swapping","score":0.6349999904632568},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.9200000166893005},{"id":"https://openalex.org/keywords/amino-acid-residues","display_name":"amino acid residues","score":0.44200000166893005},{"id":"https://openalex.org/keywords/catalytic-domain","display_name":"catalytic domain","score":0.8159999847412109},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5490000247955322},{"id":"https://openalex.org/keywords/fucosyltransferase","display_name":"fucosyltransferase","score":0.7319999933242798}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3869853615760803}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005647","descriptor_name":"Fucosyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005647","descriptor_name":"Fucosyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005647","descriptor_name":"Fucosyltransferases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.273.39.25244","doi":"https://doi.org/10.1074/jbc.273.39.25244","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.39.25244","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:9737988","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9737988","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.273.39.25244","doi":"https://doi.org/10.1074/jbc.273.39.25244","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.39.25244","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1491228191","display_name":"HUMAN FUCOSYLTRANSFERASE STRUCTURE/FUNCTION ANALYSIS","funder_award_id":"R01CA070740","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G1535832791","display_name":null,"funder_award_id":"CA70740","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G5072346692","display_name":"BIOMEDICAL RESEARCH AT SAN FRANCISCO STATE UNIVERSITY","funder_award_id":"P20RR011805","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G5238536496","display_name":"MBRS SCORE at San Francisco State University","funder_award_id":"S06GM052588","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G807401478","display_name":null,"funder_award_id":"GM 52588","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W176657936","https://openalex.org/W1522067055","https://openalex.org/W1536901694","https://openalex.org/W1553410372","https://openalex.org/W1558984579","https://openalex.org/W1968104539","https://openalex.org/W1970239328","https://openalex.org/W1978364683","https://openalex.org/W1987606627","https://openalex.org/W1994065019","https://openalex.org/W1995579116","https://openalex.org/W2015617142","https://openalex.org/W2016573683","https://openalex.org/W2017798820","https://openalex.org/W2026380980","https://openalex.org/W2040043387","https://openalex.org/W2040666939","https://openalex.org/W2041310039","https://openalex.org/W2047394974","https://openalex.org/W2047916715","https://openalex.org/W2048211449","https://openalex.org/W2050233944","https://openalex.org/W2061361468","https://openalex.org/W2064405917","https://openalex.org/W2071299534","https://openalex.org/W2076603801","https://openalex.org/W2078179498","https://openalex.org/W2079657455","https://openalex.org/W2083135897","https://openalex.org/W2099675490","https://openalex.org/W2136911663","https://openalex.org/W2137469126","https://openalex.org/W2401833872","https://openalex.org/W2989345970"],"related_works":["https://openalex.org/W2015617142","https://openalex.org/W2014098715","https://openalex.org/W2103102072","https://openalex.org/W2138498271","https://openalex.org/W4293857927","https://openalex.org/W2040666939","https://openalex.org/W2071299534","https://openalex.org/W2110572874","https://openalex.org/W1979267682","https://openalex.org/W2145812699"],"abstract_inverted_index":{"In":[0,65],"a":[1,18,24,38,163],"previous":[2],"study":[3],"(Xu,":[4],"Z.,":[5],"Vo,":[6],"L.,":[7],"and":[8,104,122],"Macher,":[9],"B.":[10],"A.":[11],"(1996)":[12],"J.":[13],"Biol.":[14],"Chem.":[15],"271,":[16],"8818-8823),":[17],"domain":[19,223],"swapping":[20],"approach":[21],"demonstrated":[22,162],"that":[23,97,152,184,191,212],"region":[25],"of":[26,76,126,153,158,220,224],"amino":[27,79,129,143,193,213,218],"acids":[28,130,194],"found":[29,185],"in":[30,41,195],"human":[31],"alpha1,":[32,47],"3/4-fucosyltransferase":[33],"III":[34,135,197,226],"(FucT":[35,50],"III)":[36],"conferred":[37,109],"significant":[39],"increase":[40],"alpha1,4-FucT":[42,61,83,99,148],"acceptor":[43,62,84,170,230],"substrate":[44,63,85,231],"specificity":[45],"into":[46,136],"3-fucosyltransferase":[48],"V":[49,112,139,160],"V),":[51],"which,":[52],"under":[53],"the":[54,66,77,127,137,217,221],"same":[55],"assay":[56],"conditions,":[57],"has":[58],"extremely":[59],"low":[60],"specificity.":[64,232],"current":[67],"study,":[68],"site-directed":[69],"mutagenesis":[70],"was":[71,179],"utilized":[72],"to":[73,120,124,151,200,228],"identify":[74],"which":[75],"eight":[78,128],"acids,":[80],"associated":[81],"with":[82,101,173],"specificity,":[86],"is/are":[87],"responsible":[88],"for":[89,166,186,205],"conferring":[90],"this":[91,178],"new":[92],"property.":[93],"The":[94],"results":[95,210],"demonstrate":[96,211],"increased":[98,147],"activity":[100,149],"both":[102],"disaccharide":[103],"glycolipid":[105],"acceptors":[106],"can":[107],"be":[108],"on":[110],"FucT":[111,134,138,154,159,175,188,196,225],"by":[113],"modifying":[114],"as":[115,117],"few":[116],"two":[118],"(Asn86":[119],"His":[121],"Thr87":[123],"Ile)":[125],"originally":[131],"swapped":[132],"from":[133],"sequence.":[140],"Neither":[141],"single":[142],"acid":[144,214],"mutant":[145],"had":[146],"relative":[150],"V.":[155,176],"Kinetic":[156],"analyses":[157],"mutants":[161],"reduced":[164],"Km":[165],"Galbeta1,3GlcNAc":[167],"(type":[168],"1)":[169],"substrates":[171],"compared":[172],"native":[174,187],"However,":[177],"about":[180],"20-fold":[181],"higher":[182],"than":[183],"III,":[189],"suggesting":[190],"other":[192],"must":[198],"contribute":[199,227],"its":[201,229],"overall":[202],"binding":[203],"site":[204],"type":[206],"1":[207],"substrates.":[208],"These":[209],"residues":[215],"near":[216],"terminus":[219],"catalytic":[222]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}