{"id":"https://openalex.org/W1994761682","doi":"https://doi.org/10.1046/j.1365-3024.1999.00191.x","title":"Biochemical characterization and localization of Fasciola hepatica 26\u201028 kDa diagnostic coproantigen","display_name":"Biochemical characterization and localization of Fasciola hepatica 26\u201028 kDa diagnostic coproantigen","publication_year":1999,"publication_date":"1999-06-01","ids":{"openalex":"https://openalex.org/W1994761682","doi":"https://doi.org/10.1046/j.1365-3024.1999.00191.x","mag":"1994761682","pmid":"https://pubmed.ncbi.nlm.nih.gov/10354350"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-3024.1999.00191.x","doi":"https://doi.org/10.1046/j.1365-3024.1999.00191.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-3024.1999.00191.x","pdf_url":null,"source":{"id":"https://openalex.org/S80886111","display_name":"Parasite Immunology","issn_l":"0141-9838","issn":["0141-9838","1365-3024"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Parasite Immunology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005789057","display_name":"Salma M. Abdel-Rahman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"SALMA ABDEL\u2010RAHMAN","raw_affiliation_strings":["Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103066252","display_name":"John B. Malone","orcid":"https://orcid.org/0000-0002-0140-5793","observed_orcids":["https://orcid.org/0000-0002-0140-5793"]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"JOHN B. MALONE","raw_affiliation_strings":["Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA","institution_ids":["https://openalex.org/I121820613"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113665712","display_name":"Kathy L. O\u2019Reilly","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121820613","display_name":"Louisiana State University","ror":"https://ror.org/05ect4e57","country_code":"US","type":"education","lineage":["https://openalex.org/I121820613"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"KATHY L. O'REILLY","raw_affiliation_strings":["Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA","institution_ids":["https://openalex.org/I121820613"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5113665712"],"corresponding_institution_ids":["https://openalex.org/I121820613"],"apc_list":{"value":4220,"currency":"USD","value_usd":4220},"apc_paid":null,"fwci":0.9557,"has_fulltext":false,"cited_by_count":21,"citation_normalized_percentile":{"value":0.70521504,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"21","issue":"6","first_page":"279","last_page":"286"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11074","display_name":"Helminth infection and control","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11074","display_name":"Helminth infection and control","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10695","display_name":"Parasites and Host Interactions","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12234","display_name":"Mollusks and Parasites Studies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fasciola-hepatica","display_name":"Fasciola hepatica","score":1.0},{"id":"https://openalex.org/keywords/diagnostic-antigen","display_name":"diagnostic antigen","score":0.6119999885559082},{"id":"https://openalex.org/keywords/glycoproteins","display_name":"glycoproteins","score":0.7070000171661377},{"id":"https://openalex.org/keywords/antigenic-characterization","display_name":"antigen characterization","score":0.3529999852180481},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.8889999985694885},{"id":"https://openalex.org/keywords/epitope-mapping","display_name":"epitope mapping","score":0.6620000004768372},{"id":"https://openalex.org/keywords/protein-core","display_name":"protein core","score":0.46399998664855957},{"id":"https://openalex.org/keywords/trypsin-cleavage","display_name":"trypsin cleavage","score":0.6240000128746033},{"id":"https://openalex.org/keywords/antigen-stability","display_name":"antigen stability","score":0.6639999747276306},{"id":"https://openalex.org/keywords/tegument","display_name":"tegument","score":0.7360000014305115}],"concepts":[{"id":"https://openalex.org/C2777856171","wikidata":"https://www.wikidata.org/wiki/Q334149","display_name":"Fasciola hepatica","level":3,"score":0.9253095388412476},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8333885669708252},{"id":"https://openalex.org/C2777228834","wikidata":"https://www.wikidata.org/wiki/Q157505","display_name":"Hepatica","level":4,"score":0.6093089580535889},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.45162808895111084},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.40858209133148193},{"id":"https://openalex.org/C165901193","wikidata":"https://www.wikidata.org/wiki/Q3129936","display_name":"Helminths","level":2,"score":0.16267427802085876}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000947","descriptor_name":"Antigens, Helminth","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000947","descriptor_name":"Antigens, Helminth","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D000947","descriptor_name":"Antigens, Helminth","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002418","descriptor_name":"Cattle Diseases","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D005210","descriptor_name":"Fasciola hepatica","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D005210","descriptor_name":"Fasciola hepatica","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D005211","descriptor_name":"Fascioliasis","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":false},{"descriptor_ui":"D005211","descriptor_name":"Fascioliasis","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D005211","descriptor_name":"Fascioliasis","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015801","descriptor_name":"Helminth Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D019084","descriptor_name":"Fluorescent Antibody Technique, Indirect","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-3024.1999.00191.x","doi":"https://doi.org/10.1046/j.1365-3024.1999.00191.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-3024.1999.00191.x","pdf_url":null,"source":{"id":"https://openalex.org/S80886111","display_name":"Parasite Immunology","issn_l":"0141-9838","issn":["0141-9838","1365-3024"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Parasite Immunology","raw_type":"journal-article"},{"id":"pmid:10354350","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10354350","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Parasite immunology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W573007399","https://openalex.org/W1553500967","https://openalex.org/W1669465679","https://openalex.org/W1912085804","https://openalex.org/W1976612483","https://openalex.org/W1977322706","https://openalex.org/W1985305423","https://openalex.org/W1989429554","https://openalex.org/W2011021229","https://openalex.org/W2017138031","https://openalex.org/W2033614267","https://openalex.org/W2035045655","https://openalex.org/W2045390893","https://openalex.org/W2071782315","https://openalex.org/W2071923015","https://openalex.org/W2075866917","https://openalex.org/W2083884411","https://openalex.org/W2088134365","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2139793378","https://openalex.org/W2296681330","https://openalex.org/W2296732095","https://openalex.org/W2396244349","https://openalex.org/W2414704451","https://openalex.org/W2434440685","https://openalex.org/W4391199943"],"related_works":["https://openalex.org/W2756947151","https://openalex.org/W2135302072","https://openalex.org/W4391199943","https://openalex.org/W2296218496","https://openalex.org/W2035045655","https://openalex.org/W2054610788","https://openalex.org/W1959141936","https://openalex.org/W2130752292","https://openalex.org/W4313011766","https://openalex.org/W269453016"],"abstract_inverted_index":{"We":[0],"have":[1],"previously":[2],"reported":[3],"the":[4,21,29,41,44,62,73,86,90,98,111,116,125,144,158,161],"usefulness":[5,167],"of":[6,11,16,31,43,48,72,139,160],"a":[7,65],"26-28":[8,22,50,99,162],"kDa":[9,23,51,80,100,163],"coproantigen":[10,24,52,63,75,103,126,145,164],"Fasciola":[12],"hepatica":[13,173],"for":[14,171],"diagnosis":[15],"infection.":[17],"In":[18],"this":[19],"study,":[20],"was":[25,64,95,119,127,146],"biochemically":[26],"characterized":[27],"with":[28,97],"aid":[30],"monoclonal":[32],"antibodies":[33],"(MoAb)":[34],"in":[35,77,148,168],"an":[36,78],"effort":[37],"to":[38,121],"better":[39],"understand":[40],"biology":[42],"antigen.":[45],"Differential":[46],"staining":[47],"chromatographically-purified":[49],"on":[53,136],"SDS-PAGE,":[54],"under":[55,129],"reducing":[56],"and":[57,151,165],"non-reducing":[58],"conditions,":[59],"indicated":[60,142],"that":[61,143],"monomeric,":[66],"highly":[67],"glycosylated":[68],"glycoprotein.":[69],"Alkaline":[70],"treatment":[71],"purified":[74],"resulted":[76],"8":[79],"protein":[81],"core":[82],"which":[83],"still":[84],"contained":[85],"epitope":[87,113],"recognized":[88,114],"by":[89,107,115],"MoAb.":[91],"No":[92],"protease":[93],"activity":[94],"associated":[96],"coproantigen.":[101],"The":[102],"could":[104],"be":[105],"cleaved":[106],"trypsin":[108],"without":[109],"altering":[110],"reactive":[112],"MoAb,":[117],"but":[118],"resistant":[120],"pepsin":[122],"digestion.":[123],"Further,":[124],"stable":[128],"several":[130],"different":[131],"storage":[132],"conditions.":[133],"Indirect":[134],"immunofluorescence":[135],"tissue":[137],"sections":[138],"adult":[140],"flukes":[141],"present":[147],"gut":[149],"cells":[150],"tegument.":[152],"Taken":[153],"together":[154],"these":[155],"results":[156],"confirm":[157],"stability":[159],"its":[166],"diagnostic":[169],"tests":[170],"F.":[172],"infections.":[174]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}