{"id":"https://openalex.org/W1994552698","doi":"https://doi.org/10.1104/pp.98.3.942","title":"Urease-Null and Hydrogenase-Null Phenotypes of a Phylloplane Bacterium Reveal Altered Nickel Metabolism in Two Soybean Mutants","display_name":"Urease-Null and Hydrogenase-Null Phenotypes of a Phylloplane Bacterium Reveal Altered Nickel Metabolism in Two Soybean Mutants","publication_year":1992,"publication_date":"1992-03-01","ids":{"openalex":"https://openalex.org/W1994552698","doi":"https://doi.org/10.1104/pp.98.3.942","mag":"1994552698","pmid":"https://pubmed.ncbi.nlm.nih.gov/16668768","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1080291"},"language":"en","primary_location":{"id":"doi:10.1104/pp.98.3.942","doi":"https://doi.org/10.1104/pp.98.3.942","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.98.3.942","pdf_url":"https://academic.oup.com/plphys/article-pdf/98/3/942/35651980/plphys_v98_3_942.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/plphys/article-pdf/98/3/942/35651980/plphys_v98_3_942.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103466119","display_name":"Mark A. Holland","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark A. Holland","raw_affiliation_strings":["Department of Biochemistry, 111 Schweitzer Hall, University of Missouri, Columbia, Missouri 65211"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, 111 Schweitzer Hall, University of Missouri, Columbia, Missouri 65211","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111702499","display_name":"Joseph Carmine Polacco","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph C. Polacco","raw_affiliation_strings":["Department of Biochemistry and Interdisciplinary Plant Group, 111 Schweitzer Hall, University of Missouri, Columbia, Missouri 65211"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Interdisciplinary Plant Group, 111 Schweitzer Hall, University of Missouri, Columbia, Missouri 65211","institution_ids":["https://openalex.org/I76835614"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I76835614"],"apc_list":{"value":3851,"currency":"USD","value_usd":3851},"apc_paid":null,"fwci":1.1159,"has_fulltext":true,"cited_by_count":112,"citation_normalized_percentile":{"value":0.70402299,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"98","issue":"3","first_page":"942","last_page":"948"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.33399999141693115,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.33399999141693115,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.2786000072956085,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11470","display_name":"Legume Nitrogen Fixing Symbiosis","score":0.1459999978542328,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/urease","display_name":"urease","score":0.8629999756813049},{"id":"https://openalex.org/keywords/hydrogenase","display_name":"hydrogenase","score":0.9750000238418579},{"id":"https://openalex.org/keywords/nickel-metabolism","display_name":"nickel metabolism","score":0.9330000281333923},{"id":"https://openalex.org/keywords/soybean-mutants","display_name":"soybean mutants","score":0.777999997138977},{"id":"https://openalex.org/keywords/methylobacterium-mesophilicum","display_name":"Methylobacterium mesophilicum","score":0.9049999713897705},{"id":"https://openalex.org/keywords/phylloplane-bacteria","display_name":"phylloplane bacteria","score":0.9879999756813049},{"id":"https://openalex.org/keywords/nickel-uptake","display_name":"nickel uptake","score":0.8759999871253967},{"id":"https://openalex.org/keywords/nickel-transport","display_name":"nickel transport","score":0.7590000033378601},{"id":"https://openalex.org/keywords/rootstock-grafting","display_name":"rootstock grafting","score":0.5090000033378601},{"id":"https://openalex.org/keywords/plant-microbe-interactions","display_name":"plant-microbe interactions","score":0.6060000061988831},{"id":"https://openalex.org/keywords/leaf-associated-bacteria","display_name":"leaf-associated bacteria","score":0.30799999833106995}],"concepts":[{"id":"https://openalex.org/C2779521917","wikidata":"https://www.wikidata.org/wiki/Q22327086","display_name":"Urease","level":3,"score":0.8951227068901062},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.7915716171264648},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.6870269775390625},{"id":"https://openalex.org/C79115784","wikidata":"https://www.wikidata.org/wiki/Q424135","display_name":"Hydrogenase","level":3,"score":0.6323602199554443},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5174351334571838},{"id":"https://openalex.org/C119795356","wikidata":"https://www.wikidata.org/wiki/Q407801","display_name":"Isozyme","level":3,"score":0.4865368902683258},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.48652926087379456},{"id":"https://openalex.org/C205626393","wikidata":"https://www.wikidata.org/wiki/Q2089263","display_name":"Phyllosphere","level":3,"score":0.46732890605926514},{"id":"https://openalex.org/C207583985","wikidata":"https://www.wikidata.org/wiki/Q128723","display_name":"Wild type","level":4,"score":0.43294256925582886},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4195787310600281},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.41752636432647705},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3315575420856476},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.24260863661766052},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1858060657978058}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1104/pp.98.3.942","doi":"https://doi.org/10.1104/pp.98.3.942","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.98.3.942","pdf_url":"https://academic.oup.com/plphys/article-pdf/98/3/942/35651980/plphys_v98_3_942.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1080291","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1080291","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC1080291/pdf/plntphys00702-0152.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiol","raw_type":"Text"},{"id":"pmid:16668768","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16668768","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant physiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1104/pp.98.3.942","doi":"https://doi.org/10.1104/pp.98.3.942","is_oa":true,"landing_page_url":"https://doi.org/10.1104/pp.98.3.942","pdf_url":"https://academic.oup.com/plphys/article-pdf/98/3/942/35651980/plphys_v98_3_942.pdf","source":{"id":"https://openalex.org/S42294715","display_name":"PLANT PHYSIOLOGY","issn_l":"0032-0889","issn":["0032-0889","1532-2548"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Physiology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.9208999872207642}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4345897713","display_name":"Genetic Regulation of Tissue-Specific Urease Isozymes","funder_award_id":"8718314","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G940395364","display_name":"Biochemical Genetics of Nitrogen Assimilation in Soybean","funder_award_id":"8804778","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320309568","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06"},{"id":"https://openalex.org/F4320311090","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1994552698.pdf","grobid_xml":"https://content.openalex.org/works/W1994552698.grobid-xml"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W655013588","https://openalex.org/W1581921573","https://openalex.org/W1858748698","https://openalex.org/W1969613688","https://openalex.org/W1973693753","https://openalex.org/W1981604045","https://openalex.org/W1984505392","https://openalex.org/W1987489753","https://openalex.org/W2002298568","https://openalex.org/W2004213764","https://openalex.org/W2019937916","https://openalex.org/W2023970230","https://openalex.org/W2024556021","https://openalex.org/W2040153694","https://openalex.org/W2041466995","https://openalex.org/W2061058399","https://openalex.org/W2085017175","https://openalex.org/W2087887310","https://openalex.org/W2093904883","https://openalex.org/W2158353626","https://openalex.org/W2159325907","https://openalex.org/W2795856504","https://openalex.org/W3213505650"],"related_works":["https://openalex.org/W2153628243","https://openalex.org/W2019937916","https://openalex.org/W2492342795","https://openalex.org/W3129206061","https://openalex.org/W2132261541","https://openalex.org/W3006058370","https://openalex.org/W2288203477","https://openalex.org/W186642468","https://openalex.org/W2023970230","https://openalex.org/W1982207642"],"abstract_inverted_index":{"Mutation":[0],"at":[1,137],"either":[2],"of":[3,21,24,32,42,59,76,162,180],"two":[4],"genetic":[5],"loci":[6],"(Eu2":[7],"or":[8,44,88,93,114,124,139,152],"Eu3)":[9],"in":[10,17,97,142,144,149],"soybean":[11,61],"(Glycine":[12],"max":[13],"[L.]":[14],"Merr.)":[15],"results":[16,105],"a":[18,33],"pleiotropic":[19],"elimination":[20],"the":[22,30,40,74,108,112],"activity":[23],"both":[25],"major":[26],"urease":[27,90,100,123],"isozymes.":[28],"Surprisingly,":[29],"phenotype":[31],"phylloplane":[34],"bacterium,":[35],"Methylobacterium":[36],"mesophilicum,":[37],"living":[38,110],"on":[39,85,89,111,168],"leaves":[41,58],"eu2/eu2":[43,113,155],"eu3-e1/eu3-e1":[45,115,157],"mutants":[46,62,91,158],"is":[47,117,128],"also":[48],"affected":[49],"by":[50,73],"these":[51,60],"plant":[52],"mutations.":[53],"The":[54,81],"bacteria":[55],"isolated":[56],"from":[57],"have":[63],"transient":[64],"urease-":[65],"and":[66,156,185],"hydrogenase-deficient":[67],"phenotypes":[68],"that":[69,107,135],"can":[70],"be":[71],"corrected":[72],"addition":[75],"nickel":[77,145,181],"to":[78,119],"free-living":[79],"cultures.":[80],"same":[82],"bacterium":[83,109],"growing":[84],"wild-type":[86,165],"soybeans":[87],"eu1-sun/eu1-sun":[92],"eu4/eu4,":[94],"each":[95],"deficient":[96],"only":[98],"one":[99],"isozyme,":[101],"are":[102],"urease-positive.":[103],"These":[104],"suggest":[106],"mutant":[116,169,183],"unable":[118],"produce":[120,171],"an":[121],"active":[122,175],"hydrogenase":[125],"because":[126,154],"it":[127],"effectively":[129],"starved":[130],"for":[131],"nickel.":[132],"We":[133],"infer":[134],"mutations":[136],"Eu2":[138],"Eu3":[140],"result":[141],"defects":[143],"metabolism":[146],"but":[147],"not":[148],"Ni(2+)":[150],"uptake":[151,161],"transport,":[153],"exhibit":[159],"normal":[160],"(63)NiCl(2).":[163],"Moreover,":[164],"plants":[166],"grafted":[167],"rootstocks":[170],"seeds":[172],"with":[173],"fully":[174],"urease,":[176],"indicating":[177],"unimpeded":[178],"transport":[179],"through":[182],"roots":[184],"stems.":[186]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}