{"id":"https://openalex.org/W1994066779","doi":"https://doi.org/10.1271/bbb.69.2349","title":"Screening for New Hydroxynitrilases from Plants","display_name":"Screening for New Hydroxynitrilases from Plants","publication_year":2005,"publication_date":"2005-01-01","ids":{"openalex":"https://openalex.org/W1994066779","doi":"https://doi.org/10.1271/bbb.69.2349","mag":"1994066779","pmid":"https://pubmed.ncbi.nlm.nih.gov/16377893"},"language":"en","primary_location":{"id":"doi:10.1271/bbb.69.2349","doi":"https://doi.org/10.1271/bbb.69.2349","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.69.2349","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066929731","display_name":"Yasuhisa Asano","orcid":"https://orcid.org/0000-0003-3645-3952","observed_orcids":["https://orcid.org/0000-0003-3645-3952"]},"institutions":[{"id":"https://openalex.org/I42766147","display_name":"University of Toyama","ror":"https://ror.org/0445phv87","country_code":"JP","type":"education","lineage":["https://openalex.org/I42766147"]},{"id":"https://openalex.org/I63216439","display_name":"Toyama Prefectural University","ror":"https://ror.org/03xgh2v50","country_code":"JP","type":"education","lineage":["https://openalex.org/I63216439"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yasuhisa ASANO","raw_affiliation_strings":["Biotechnology Research Center, Toyama Prefectural University, Japan. asano@pu-toyama.ac.jp","Biotechnology Research Center, Toyama Prefectural University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Toyama Prefectural University, Japan. asano@pu-toyama.ac.jp","institution_ids":["https://openalex.org/I63216439"]},{"raw_affiliation_string":"Biotechnology Research Center, Toyama Prefectural University","institution_ids":["https://openalex.org/I42766147","https://openalex.org/I63216439"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051669115","display_name":"Ken\u2019ichirou Tamura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I42766147","display_name":"University of Toyama","ror":"https://ror.org/0445phv87","country_code":"JP","type":"education","lineage":["https://openalex.org/I42766147"]},{"id":"https://openalex.org/I63216439","display_name":"Toyama Prefectural University","ror":"https://ror.org/03xgh2v50","country_code":"JP","type":"education","lineage":["https://openalex.org/I63216439"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ken\u2019ichirou TAMURA","raw_affiliation_strings":["Biotechnology Research Center, Toyama Prefectural University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Toyama Prefectural University","institution_ids":["https://openalex.org/I42766147","https://openalex.org/I63216439"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072826060","display_name":"Nobutaka Doi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I42766147","display_name":"University of Toyama","ror":"https://ror.org/0445phv87","country_code":"JP","type":"education","lineage":["https://openalex.org/I42766147"]},{"id":"https://openalex.org/I63216439","display_name":"Toyama Prefectural University","ror":"https://ror.org/03xgh2v50","country_code":"JP","type":"education","lineage":["https://openalex.org/I63216439"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Nobutaka DOI","raw_affiliation_strings":["Biotechnology Research Center, Toyama Prefectural University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Toyama Prefectural University","institution_ids":["https://openalex.org/I42766147","https://openalex.org/I63216439"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032371530","display_name":"Techawaree Ueatrongchit","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I131868736","display_name":"Prince of Songkla University","ror":"https://ror.org/0575ycz84","country_code":"TH","type":"education","lineage":["https://openalex.org/I131868736"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Techawaree UEATRONGCHIT","raw_affiliation_strings":["Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University","institution_ids":["https://openalex.org/I131868736"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039918444","display_name":"Aran H\u2010Kittikun","orcid":"https://orcid.org/0000-0001-6383-7973","observed_orcids":["https://orcid.org/0000-0001-6383-7973"]},"institutions":[{"id":"https://openalex.org/I131868736","display_name":"Prince of Songkla University","ror":"https://ror.org/0575ycz84","country_code":"TH","type":"education","lineage":["https://openalex.org/I131868736"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Aran H-KITTIKUN","raw_affiliation_strings":["Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University","institution_ids":["https://openalex.org/I131868736"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5038841811","display_name":"Tohru Ohmiya","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405255561","display_name":"Botanic Gardens of Toyama","ror":"https://ror.org/03558c952","country_code":"JP","type":"archive","lineage":["https://openalex.org/I4405255561"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tohru OHMIYA","raw_affiliation_strings":["Botanic Gardens of Toyama"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botanic Gardens of Toyama","institution_ids":["https://openalex.org/I4405255561"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3272,"currency":"USD","value_usd":3272},"apc_paid":null,"fwci":1.0324,"has_fulltext":false,"cited_by_count":78,"citation_normalized_percentile":{"value":0.70611841,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"69","issue":"12","first_page":"2349","last_page":"2357"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9688000082969666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9688000082969666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12305","display_name":"Cassava research and cyanide","score":0.012000000104308128,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hydroxynitrile-lyase","display_name":"hydroxynitrile lyase","score":0.9459999799728394},{"id":"https://openalex.org/keywords/mandelonitrile","display_name":"mandelonitrile","score":0.7990000247955322},{"id":"https://openalex.org/keywords/cyanohydrin-synthesis","display_name":"cyanohydrin synthesis","score":0.6959999799728394},{"id":"https://openalex.org/keywords/enantiomeric-excess","display_name":"enantiomeric excess","score":0.7179999947547913},{"id":"https://openalex.org/keywords/passiflora-edulis","display_name":"Passiflora edulis","score":0.9100000262260437},{"id":"https://openalex.org/keywords/eriobotrya-japonica","display_name":"Eriobotrya japonica","score":0.9959999918937683},{"id":"https://openalex.org/keywords/baliospermum-montanum","display_name":"Baliospermum montanum","score":0.9700000286102295},{"id":"https://openalex.org/keywords/prunus-mume","display_name":"Prunus mume","score":0.7170000076293945},{"id":"https://openalex.org/keywords/peach","display_name":"peach","score":0.9480000138282776},{"id":"https://openalex.org/keywords/sorbus-aucuparia","display_name":"rowan","score":0.9890000224113464},{"id":"https://openalex.org/keywords/benzaldehyde","display_name":"benzaldehyde","score":0.6759999990463257}],"concepts":[{"id":"https://openalex.org/C2779392372","wikidata":"https://www.wikidata.org/wiki/Q1550170","display_name":"Eriobotrya","level":3,"score":0.9364944696426392},{"id":"https://openalex.org/C2779510327","wikidata":"https://www.wikidata.org/wiki/Q161185","display_name":"Passiflora","level":2,"score":0.7870025634765625},{"id":"https://openalex.org/C2776301351","wikidata":"https://www.wikidata.org/wiki/Q157164","display_name":"Passifloraceae","level":3,"score":0.778839111328125},{"id":"https://openalex.org/C2779409272","wikidata":"https://www.wikidata.org/wiki/Q1757789","display_name":"Japonica","level":2,"score":0.702417254447937},{"id":"https://openalex.org/C2781274105","wikidata":"https://www.wikidata.org/wiki/Q410215","display_name":"Amygdalin","level":3,"score":0.6546800136566162},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5541771054267883},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.5347195267677307},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.32053256034851074},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3143906593322754},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000097","descriptor_name":"Acetonitriles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000619","descriptor_name":"Aminohydrolases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000619","descriptor_name":"Aminohydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001547","descriptor_name":"Benzaldehydes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D009570","descriptor_name":"Nitriles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010422","descriptor_name":"Pentanones","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010936","descriptor_name":"Plant Extracts","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D011190","descriptor_name":"Potassium Cyanide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012639","descriptor_name":"Seeds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013237","descriptor_name":"Stereoisomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D027861","descriptor_name":"Prunus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1271/bbb.69.2349","doi":"https://doi.org/10.1271/bbb.69.2349","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.69.2349","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},{"id":"pmid:16377893","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16377893","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, biotechnology, and biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W154196214","https://openalex.org/W1522210407","https://openalex.org/W1586281663","https://openalex.org/W1967094538","https://openalex.org/W1974575939","https://openalex.org/W1981116520","https://openalex.org/W1997471595","https://openalex.org/W2007677383","https://openalex.org/W2012913801","https://openalex.org/W2014386639","https://openalex.org/W2024221445","https://openalex.org/W2026401270","https://openalex.org/W2027897531","https://openalex.org/W2028014639","https://openalex.org/W2034353893","https://openalex.org/W2035578666","https://openalex.org/W2045382679","https://openalex.org/W2048305270","https://openalex.org/W2057699146","https://openalex.org/W2063648855","https://openalex.org/W2065428674","https://openalex.org/W2066569533","https://openalex.org/W2066776353","https://openalex.org/W2086721453","https://openalex.org/W2115045203","https://openalex.org/W2128635872","https://openalex.org/W2410040075","https://openalex.org/W2492719969","https://openalex.org/W2949100716","https://openalex.org/W2950531672","https://openalex.org/W3120713190"],"related_works":["https://openalex.org/W2064404757","https://openalex.org/W4400259440","https://openalex.org/W3006213014","https://openalex.org/W2012913801","https://openalex.org/W2849035367","https://openalex.org/W4409349319","https://openalex.org/W2004107044","https://openalex.org/W2059019629","https://openalex.org/W2808575763","https://openalex.org/W2052704730"],"abstract_inverted_index":{"We":[0,54],"established":[1],"a":[2],"simple":[3],"HPLC":[4],"method":[5],"to":[6,24],"determine":[7],"the":[8,13,52],"activity":[9,30],"and":[10,19,21,48,68,73,84,96,127],"stereochemistry":[11],"of":[12,31,36,39,58,60,112,122,129,136],"chiral":[14],"mandelonitrile":[15],"synthesized":[16,115,131],"from":[17,70,75,93,116,120,134],"benzaldehyde":[18,103],"cyanide,":[20],"applied":[22],"it":[23],"screen":[25],"for":[26,46],"hydroxynitrile":[27],"lyase":[28],"(HNL)":[29],"plant":[32],"origin.":[33],"A":[34],"total":[35],"163":[37],"species":[38],"plants":[40],"among":[41],"74":[42],"families":[43],"were":[44],"examined":[45],"(R)-":[47],"(S)-HNL":[49,64],"activities":[50],"using":[51,118],"method.":[53],"discovered":[55],"that":[56,128],"homogenate":[57,119,133],"leaves":[59,67,121],"Baliospermum":[61],"montanum":[62],"shows":[63],"activity,":[65],"while":[66],"seeds":[69,74,135],"Passiflora":[71,94,123],"edulis,":[72],"Eriobotrya":[76,97,137],"japonica,":[77],"Chaenomles":[78],"sinensis,":[79],"Sorbus":[80],"aucuparia,":[81],"Prunus":[82,85],"mume,":[83],"persica":[86],"show":[87],"(R)-HNL":[88],"activity.":[89],"Partially":[90],"purified":[91],"(R)-HNLs":[92],"edulis":[95,124],"japonica":[98,138],"acted":[99],"not":[100],"only":[101],"on":[102,106],"but":[104],"also":[105],"aliphatic":[107],"ketone.":[108],"The":[109],"enantiomeric":[110],"excess":[111],"(R)-methylpropylketone":[113],"cyanohydrin":[114],"2-pentanone":[117],"was":[125,139],"87.0%,":[126],"(R)-mandelonitrile":[130],"by":[132],"85.0%.":[140]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":5}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}