{"id":"https://openalex.org/W1993973987","doi":"https://doi.org/10.1021/ja061036f","title":"Site-Specific Cleavage of RNA by a Metal-Free Artificial Nuclease Attached to Antisense Oligonucleotides","display_name":"Site-Specific Cleavage of RNA by a Metal-Free Artificial Nuclease Attached to Antisense Oligonucleotides","publication_year":2006,"publication_date":"2006-05-25","ids":{"openalex":"https://openalex.org/W1993973987","doi":"https://doi.org/10.1021/ja061036f","mag":"1993973987","pmid":"https://pubmed.ncbi.nlm.nih.gov/16771522"},"language":"en","primary_location":{"id":"doi:10.1021/ja061036f","doi":"https://doi.org/10.1021/ja061036f","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja061036f","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044247374","display_name":"Claudio Gnaccarini","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Claudio Gnaccarini","raw_affiliation_strings":["Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053456384","display_name":"Sascha Peter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Sascha Peter","raw_affiliation_strings":["Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058865699","display_name":"Ute Scheffer","orcid":"https://orcid.org/0000-0002-5921-640X","observed_orcids":["https://orcid.org/0000-0002-5921-640X"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ute Scheffer","raw_affiliation_strings":["Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067409531","display_name":"Stefan Vonhoff","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Stefan Vonhoff","raw_affiliation_strings":["Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115588097","display_name":"Sven Klu\u00dfmann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Sven Klussmann","raw_affiliation_strings":["Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5042442059","display_name":"Michael G\u00f6bel","orcid":"https://orcid.org/0000-0002-5694-4823","observed_orcids":["https://orcid.org/0000-0002-5694-4823"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Michael W. G\u00f6bel","raw_affiliation_strings":["Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Contribution from the Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Marie-Curie-Str. 11, D-60439 Frankfurt am Main, Germany, and NOXXON Pharma AG, Max-Dohrn-Str. 8-10, D-10589 Berlin, Germany","institution_ids":["https://openalex.org/I114090438"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I114090438"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.2456,"has_fulltext":false,"cited_by_count":87,"citation_normalized_percentile":{"value":0.87117315,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"128","issue":"24","first_page":"8063","last_page":"8067"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.7567999958992004,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.7567999958992004,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Physical Organic Chemistry and Reaction Kinetics","score":0.0723000019788742,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.03590000048279762,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/site-specific-rna-cleavage","display_name":"site-specific RNA cleavage","score":0.8539999723434448},{"id":"https://openalex.org/keywords/artificial-nucleases","display_name":"artificial nucleases","score":0.8569999933242798},{"id":"https://openalex.org/keywords/organocatalysis","display_name":"organocatalysis","score":0.7680000066757202},{"id":"https://openalex.org/keywords/metal-free-catalysis","display_name":"metal-free catalysis","score":0.7860000133514404},{"id":"https://openalex.org/keywords/rna-degradation","display_name":"RNA degradation","score":0.6330000162124634},{"id":"https://openalex.org/keywords/ph-dependent-catalysis","display_name":"pH-dependent catalysis","score":0.7760000228881836},{"id":"https://openalex.org/keywords/saturation-kinetics","display_name":"saturation kinetics","score":0.6949999928474426},{"id":"https://openalex.org/keywords/rna-half-life","display_name":"RNA half-life","score":0.531000018119812}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8994982838630676},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.7685291767120361},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.7620728611946106},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.648809552192688},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.5712389945983887},{"id":"https://openalex.org/C84211826","wikidata":"https://www.wikidata.org/wiki/Q5260078","display_name":"Deoxyribozyme","level":3,"score":0.5539844036102295},{"id":"https://openalex.org/C197336794","wikidata":"https://www.wikidata.org/wiki/Q5161150","display_name":"Conjugate","level":2,"score":0.48474428057670593},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.48133811354637146},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.4800897240638733},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4796465039253235},{"id":"https://openalex.org/C2778976237","wikidata":"https://www.wikidata.org/wiki/Q422523","display_name":"Ribonuclease","level":4,"score":0.4585634469985962},{"id":"https://openalex.org/C99611785","wikidata":"https://www.wikidata.org/wiki/Q6453233","display_name":"Duplex (building)","level":3,"score":0.4358447790145874},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.42504674196243286},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22228720784187317},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0},{"id":"https://openalex.org/C187320778","wikidata":"https://www.wikidata.org/wiki/Q1349130","display_name":"Geotechnical engineering","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000577","descriptor_name":"Amides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000577","descriptor_name":"Amides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001562","descriptor_name":"Benzimidazoles","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004220","descriptor_name":"Disulfides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004220","descriptor_name":"Disulfides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008670","descriptor_name":"Metals","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016376","descriptor_name":"Oligonucleotides, Antisense","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016376","descriptor_name":"Oligonucleotides, Antisense","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/ja061036f","doi":"https://doi.org/10.1021/ja061036f","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja061036f","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"pmid:16771522","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16771522","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W1964164090","https://openalex.org/W1977642913","https://openalex.org/W2011041591","https://openalex.org/W2024814678","https://openalex.org/W2037714379","https://openalex.org/W2072779644","https://openalex.org/W2158694685"],"related_works":["https://openalex.org/W2022234776","https://openalex.org/W2916599067","https://openalex.org/W4409225142","https://openalex.org/W1977642913","https://openalex.org/W3016980471","https://openalex.org/W350250454","https://openalex.org/W7096319863","https://openalex.org/W2142060638","https://openalex.org/W4384563180","https://openalex.org/W3138950165"],"abstract_inverted_index":{"RNA":[0,49,68],"cleaving":[1],"tris(2-aminobenzimidazoles)":[2,80],"have":[3],"been":[4],"attached":[5],"to":[6],"DNA":[7],"oligonucleotides":[8],"via":[9],"disulfide":[10],"or":[11],"amide":[12],"bonds.":[13],"The":[14,31,51],"resulting":[15],"conjugates":[16,33,78],"are":[17,70],"effective":[18],"organocatalytic":[19],"nucleases":[20],"showing":[21],"substrate":[22],"and":[23],"site":[24],"selectivity":[25],"as":[26,28,44],"well":[27],"saturation":[29],"kinetics.":[30],"benzimidazole":[32],"also":[34],"degrade":[35],"enantiomeric":[36],"RNA.":[37],"This":[38],"observation":[39],"rules":[40],"out":[41],"contamination":[42],"effects":[43],"an":[45],"alternative":[46],"explanation":[47],"of":[48,67,74,79,86],"degradation.":[50],"pH":[52],"dependency":[53],"shows":[54],"that":[55],"the":[56,62,72,84],"catalyst":[57],"is":[58],"most":[59],"active":[60],"in":[61,71],"deprotonated":[63],"state.":[64],"Typical":[65],"half-lifes":[66],"substrates":[69],"range":[73],"12-17":[75],"h.":[76],"Thus,":[77],"can":[81],"compete":[82],"with":[83],"majority":[85],"metal-dependent":[87],"artificial":[88],"nucleases.":[89]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":10},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}