{"id":"https://openalex.org/W1993067142","doi":"https://doi.org/10.1016/0165-022x(91)90071-4","title":"Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli","display_name":"Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli","publication_year":1991,"publication_date":"1991-04-01","ids":{"openalex":"https://openalex.org/W1993067142","doi":"https://doi.org/10.1016/0165-022x(91)90071-4","mag":"1993067142","pmid":"https://pubmed.ncbi.nlm.nih.gov/1865054"},"language":"en","primary_location":{"id":"doi:10.1016/0165-022x(91)90071-4","doi":"https://doi.org/10.1016/0165-022x(91)90071-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0165-022x(91)90071-4","pdf_url":null,"source":{"id":"https://openalex.org/S3689565","display_name":"Journal of Biochemical and Biophysical Methods","issn_l":"0165-022X","issn":["0165-022X","1872-857X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biochemical and Biophysical Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5047995082","display_name":"Paul F. Lindholm","orcid":"https://orcid.org/0000-0003-2498-6372","observed_orcids":["https://orcid.org/0000-0003-2498-6372"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Paul F. Lindholm","raw_affiliation_strings":["Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892","National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017090665","display_name":"Susan J. Marriott","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan J. Marriott","raw_affiliation_strings":["Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A","National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001144056","display_name":"Scott D. Gitlin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott D. Gitlin","raw_affiliation_strings":["Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A","National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109269727","display_name":"John N Brady","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John N. Brady","raw_affiliation_strings":["Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A","National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"raw_affiliation_string":"National Institutes of Health","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5047995082"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210140884"],"apc_list":null,"apc_paid":null,"fwci":0.7928,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.68869662,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"22","issue":"3","first_page":"233","last_page":"241"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.8866999745368958,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.8866999745368958,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12159","display_name":"T-cell and Retrovirus Studies","score":0.10080000013113022,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.7820000052452087},{"id":"https://openalex.org/keywords/ammonium-sulfate-precipitation","display_name":"ammonium sulfate precipitation","score":0.9829999804496765},{"id":"https://openalex.org/keywords/recombinant-protein-expression-in-escherichia-coli","display_name":"recombinant expression in Escherichia coli","score":0.8889999985694885},{"id":"https://openalex.org/keywords/dna-binding-activity","display_name":"DNA-binding activity","score":0.7770000100135803},{"id":"https://openalex.org/keywords/biologically-active-proteins","display_name":"biologically active proteins","score":0.6050000190734863}],"concepts":[{"id":"https://openalex.org/C535196362","wikidata":"https://www.wikidata.org/wiki/Q758","display_name":"Zinc","level":2,"score":0.7162690162658691},{"id":"https://openalex.org/C2776428949","wikidata":"https://www.wikidata.org/wiki/Q18814659","display_name":"Ammonium sulfate precipitation","level":4,"score":0.6728143095970154},{"id":"https://openalex.org/C197404232","wikidata":"https://www.wikidata.org/wiki/Q319827","display_name":"Chelation","level":2,"score":0.6655294299125671},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6598072648048401},{"id":"https://openalex.org/C116073593","wikidata":"https://www.wikidata.org/wiki/Q864491","display_name":"Biological activity","level":3,"score":0.6111603379249573},{"id":"https://openalex.org/C107054158","wikidata":"https://www.wikidata.org/wiki/Q25257","display_name":"Precipitation","level":2,"score":0.601203978061676},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.5789464712142944},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5759362578392029},{"id":"https://openalex.org/C2780925461","wikidata":"https://www.wikidata.org/wiki/Q191831","display_name":"Ammonium sulfate","level":2,"score":0.5297478437423706},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4806731641292572},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4253408908843994},{"id":"https://openalex.org/C51724486","wikidata":"https://www.wikidata.org/wiki/Q908955","display_name":"Column chromatography","level":2,"score":0.42390722036361694},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4150102138519287},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.3810463547706604},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.14343217015266418},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000645","descriptor_name":"Ammonium Sulfate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002712","descriptor_name":"Chlorides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011232","descriptor_name":"Chemical Precipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015032","descriptor_name":"Zinc","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015368","descriptor_name":"Human T-lymphotropic virus 1","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016328","descriptor_name":"NF-kappa B","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D016328","descriptor_name":"NF-kappa B","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016356","descriptor_name":"Gene Products, tax","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016356","descriptor_name":"Gene Products, tax","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D017967","descriptor_name":"Zinc Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/0165-022x(91)90071-4","doi":"https://doi.org/10.1016/0165-022x(91)90071-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0165-022x(91)90071-4","pdf_url":null,"source":{"id":"https://openalex.org/S3689565","display_name":"Journal of Biochemical and Biophysical Methods","issn_l":"0165-022X","issn":["0165-022X","1872-857X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biochemical and Biophysical Methods","raw_type":"journal-article"},{"id":"pmid:1865054","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1865054","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biochemical and biophysical methods","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W113625048","https://openalex.org/W1538445776","https://openalex.org/W1593631074","https://openalex.org/W1892064464","https://openalex.org/W1965168699","https://openalex.org/W1975104035","https://openalex.org/W1991941671","https://openalex.org/W2002601205","https://openalex.org/W2006244184","https://openalex.org/W2019698681","https://openalex.org/W2026266426","https://openalex.org/W2029291655","https://openalex.org/W2031089158","https://openalex.org/W2032496145","https://openalex.org/W2038586333","https://openalex.org/W2043161252","https://openalex.org/W2060641378","https://openalex.org/W2069667541","https://openalex.org/W2088261928","https://openalex.org/W2093665411","https://openalex.org/W2095516251","https://openalex.org/W2106283298","https://openalex.org/W2131606860","https://openalex.org/W2470687740","https://openalex.org/W6658728845"],"related_works":["https://openalex.org/W2604584627","https://openalex.org/W2108563249","https://openalex.org/W2087745262","https://openalex.org/W2272229712","https://openalex.org/W1963770739","https://openalex.org/W2610725861","https://openalex.org/W1975895675","https://openalex.org/W2413866361","https://openalex.org/W2082584047","https://openalex.org/W94919756"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}