{"id":"https://openalex.org/W1991995635","doi":"https://doi.org/10.4141/cjas86-017","title":"A RAPID PROCEDURE FOR PURINE MEASUREMENT AND ITS USE FOR ESTIMATING NET RUMINAL PROTEIN SYNTHESIS","display_name":"A RAPID PROCEDURE FOR PURINE MEASUREMENT AND ITS USE FOR ESTIMATING NET RUMINAL PROTEIN SYNTHESIS","publication_year":1986,"publication_date":"1986-03-01","ids":{"openalex":"https://openalex.org/W1991995635","doi":"https://doi.org/10.4141/cjas86-017","mag":"1991995635"},"language":"en","primary_location":{"id":"doi:10.4141/cjas86-017","doi":"https://doi.org/10.4141/cjas86-017","is_oa":false,"landing_page_url":"https://doi.org/10.4141/cjas86-017","pdf_url":null,"source":{"id":"https://openalex.org/S54036783","display_name":"Canadian Journal of Animal Science","issn_l":"0008-3984","issn":["0008-3984","1918-1825"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian Journal of Animal Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078607665","display_name":"Richard Avery Zinn","orcid":"https://orcid.org/0000-0003-3014-3814","observed_orcids":["https://orcid.org/0000-0003-3014-3814"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R. A. ZINN","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5026300567","display_name":"Fredric N. Owens","orcid":"https://orcid.org/0009-0000-4329-9629","observed_orcids":["https://orcid.org/0009-0000-4329-9629"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"F. N. OWENS","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":18.4078,"has_fulltext":false,"cited_by_count":815,"citation_normalized_percentile":{"value":0.99760431,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":98,"max":100},"biblio":{"volume":"66","issue":"1","first_page":"157","last_page":"166"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10098","display_name":"Ruminant Nutrition and Digestive Physiology","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10098","display_name":"Ruminant Nutrition and Digestive Physiology","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10152","display_name":"Animal Nutrition and Physiology","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14127","display_name":"Food and Agricultural Sciences","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/single-cell-protein","display_name":"microbial protein","score":0.7409999966621399},{"id":"https://openalex.org/keywords/ruminal-digesta","display_name":"ruminal digesta","score":0.8240000009536743},{"id":"https://openalex.org/keywords/intestinal-digesta","display_name":"intestinal digesta","score":0.8069999814033508},{"id":"https://openalex.org/keywords/silver-nitrate-precipitation","display_name":"silver nitrate precipitation","score":0.746999979019165},{"id":"https://openalex.org/keywords/perchloric-acid-hydrolysis","display_name":"perchloric acid hydrolysis","score":0.8669999837875366},{"id":"https://openalex.org/keywords/steers","display_name":"steers","score":0.7020000219345093},{"id":"https://openalex.org/keywords/ruminant-nutrition","display_name":"ruminant nutrition","score":0.5370000004768372}],"concepts":[{"id":"https://openalex.org/C169586020","wikidata":"https://www.wikidata.org/wiki/Q3780159","display_name":"Purine metabolism","level":3,"score":0.9077193737030029},{"id":"https://openalex.org/C2776476023","wikidata":"https://www.wikidata.org/wiki/Q188261","display_name":"Purine","level":3,"score":0.6917912364006042},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5603346228599548},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5367727279663086},{"id":"https://openalex.org/C70262065","wikidata":"https://www.wikidata.org/wiki/Q427652","display_name":"Rumen","level":3,"score":0.5181133151054382},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.4703531265258789},{"id":"https://openalex.org/C2779446143","wikidata":"https://www.wikidata.org/wiki/Q416538","display_name":"Tungstic acid","level":3,"score":0.46458035707473755},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.45381447672843933},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4282945990562439},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3443644046783447},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11277076601982117},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.10735759139060974},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.4141/cjas86-017","doi":"https://doi.org/10.4141/cjas86-017","is_oa":false,"landing_page_url":"https://doi.org/10.4141/cjas86-017","pdf_url":null,"source":{"id":"https://openalex.org/S54036783","display_name":"Canadian Journal of Animal Science","issn_l":"0008-3984","issn":["0008-3984","1918-1825"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310312929","host_organization_name":"Canadian Science Publishing","host_organization_lineage":["https://openalex.org/P4310312929"],"host_organization_lineage_names":["Canadian Science Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian Journal of Animal Science","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.40450000762939453,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":2,"referenced_works":["https://openalex.org/W1659737639","https://openalex.org/W2018280568"],"related_works":["https://openalex.org/W2385271035","https://openalex.org/W2107747422","https://openalex.org/W2371465765","https://openalex.org/W4255995608","https://openalex.org/W1980154822","https://openalex.org/W1986992666","https://openalex.org/W2383620246","https://openalex.org/W246405097","https://openalex.org/W3211419155","https://openalex.org/W3152875040"],"abstract_inverted_index":{"A":[0],"rapid":[1],"method":[2],"for":[3,16,31,95,132],"separation":[4],"and":[5,13,83,99],"quantitation":[6],"of":[7,33,41,70,74,88,117],"purines":[8,43,50,56,71,125],"was":[9,65,105],"applied":[10],"to":[11,47,72],"ruminal":[12],"intestinal":[14],"digesta":[15],"estimating":[17],"net":[18],"microbial":[19,89,134,141],"protein":[20,64,104,142],"synthesis":[21],"in":[22],"the":[23,49,68],"rumen.":[24],"The":[25,77],"procedure":[26,78,94],"combines":[27],"standard":[28],"literature":[29],"methods":[30],"hydrolysis":[32],"nucleotides":[34],"by":[35,39,67,92],"perchloric":[36],"acid":[37,119],"followed":[38],"precipitation":[40],"free":[42],"with":[44,114],"silver":[45],"nitrate":[46],"separate":[48],"from":[51],"interfering":[52],"compounds.":[53],"Acid":[54],"resolubilized":[55],"were":[57],"quantitated":[58],"spectrophotometrically":[59],"at":[60],"260":[61],"nm.":[62],"Microbial":[63],"estimated":[66,91],"ratio":[69],"N":[73,90],"isolated":[75],"bacteria.":[76],"is":[79],"rapid,":[80],"simple,":[81],"precise":[82],"not":[84],"costly.":[85],"Duodenal":[86],"passage":[87,116],"this":[93],"steers":[96],"fed":[97],"semipurified":[98],"purified":[100],"diets":[101],"containing":[102],"no":[103],"highly":[106],"correlated":[107],"(R2":[108],"=":[109],"0.98;":[110],"P":[111],"<":[112],"0.01)":[113],"duodenal":[115],"tungstic":[118],"precipitable":[120],"N.":[121],"Results":[122],"indicate":[123],"that":[124],"may":[126],"be":[127],"useful":[128],"as":[129],"a":[130],"marker":[131],"quantitating":[133],"protein.":[135],"Key":[136],"words:":[137],"Purine,":[138],"RNA,":[139],"DNA,":[140]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":15},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":16},{"year":2022,"cited_by_count":17},{"year":2021,"cited_by_count":16},{"year":2020,"cited_by_count":10},{"year":2019,"cited_by_count":21},{"year":2018,"cited_by_count":15},{"year":2017,"cited_by_count":19},{"year":2016,"cited_by_count":17},{"year":2015,"cited_by_count":12},{"year":2014,"cited_by_count":27},{"year":2013,"cited_by_count":27},{"year":2012,"cited_by_count":24}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}